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Gene information
General information
TC015229 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9336775..9338711
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015229
    iBeetle screen iB_02436

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015229 RA
      MELSIEQGR LRGCVGTDI KGSNFLKFK GIPYARPPL 
      GDLRFKAPQ PPEKWAGVL DASKEGDAS YHCDVVREN 
      IMVGSENCL VLNVYTRKL GDGKNLRPV MVWIHGGGF 
      VWGSGSEEL YGPDFLMTE DIVYVTINY RLGMLGFLN 
      FEDPSLGVP GNAGFKDMI MGLKWVQRN ISAFGGDPN 
      NVTIFGESA GGASVHLLT LSPLAKGLF HKAIVQSAC 
      ALNPGARGT KGARHIAKA LNLEGADDK TVLEHLKTK 
      TFAEIFEIQ KKIVDPLVA NEIRPFGPV VESGDKKAA 
      FLTQEPYEI ILSGNFNQV PVMIGFTSR EGMLAQMFK 
      DCRKDFSVQ DHIPWTFGY SKEEAKAVT TTVKKFYFG 
      NEEPSADNV VKAFDLASD VFFLYGIHK AVGAQYSRS 
      KAPIYVYEM TIETELNFF KNLLKITEP GVCHADDIG 
      YLFNHFVTP KITPGSAAD IGRSRFVKL WTNFAKFGN 
      PTPDQNDKL LKTVWKPVT KPEIDVLDI GTDLKMVSN 
      PYRERIQFW EKLYSESPA GRNK
    CDS sequence
    • >TC015229 RA
      atggagtta tcaatagaa caggggagg cttcgaggg 
      tgtgtcggg actgacatc aaagggagc aattttctc 
      aaattcaag ggaatccca tatgcgagg cccccgctg 
      ggtgacctc cgattcaag gcgccccaa cctccggaa 
      aaatgggct ggtgtttta gacgcgtcc aaagaaggg 
      gatgcttcc taccactgc gacgtagtc agagagaat 
      atcatggtt gggagcgaa aactgtctg gtcttgaac 
      gtgtacacg cgaaaactg ggtgatggt aaaaatttg 
      cgcccagtc atggtttgg atccacggg ggtggtttt 
      gtttggggc tccgggtct gaagaacta tacggccct 
      gatttccta atgaccgaa gatatcgtc tacgttacc 
      ataaattat cggttggga atgttgggt tttttgaac 
      tttgaggac ccgtccttg ggggtgcct ggcaatgcc 
      gggttcaag gacatgata atgggttta aaatgggtc 
      cagaggaat atttcggct ttcgggggt gaccccaac 
      aatgttaca attttcggg gagagtgcc gggggtgcg 
      tctgtgcat ttgctaaca ctgtcacct ctggcaaaa 
      ggtcttttt cacaaagcc attgttcag agtgcctgt 
      gcattgaac ccgggggct agaggcacg aaaggggcg 
      cgtcatatt gccaaggct ttgaacctt gaaggggcc 
      gatgataag acagttttg gagcatttg aagactaaa 
      actttcgcc gagattttt gaaattcag aaaaaaatt 
      gttgatcct ctggtggct aatgagatt cgaccgttt 
      gggcctgtg gttgaatct ggtgacaaa aaagcggct 
      tttttgaca caagaaccg tacgaaatt atattatca 
      gggaatttc aatcaagtt cccgttatg attgggttt 
      acgtcgcgt gaaggaatg ttggcccaa atgtttaaa 
      gattgtcgt aaggacttt tctgttcag gatcacatt 
      ccttggacg tttgggtat agtaaggag gaagcgaaa 
      gctgttacc actacagtc aaaaaattc tactttggt 
      aatgaggag ccatcagcg gataatgtt gtaaaagca 
      tttgatttg gcaagcgac gttttcttt ctttatggt 
      attcacaaa gctgttggg gctcaatac agtcggtct 
      aaagcacca atctacgta tacgaaatg acaatcgaa 
      acagaactg aactttttc aagaaccta ctaaaaatt 
      acagaacct ggtgtttgt cacgctgac gacattggt 
      tatctcttc aatcatttt gtaactccg aaaataact 
      cccggaagc gccgcagat atcggtcgt tcaaggttc 
      gtcaagttg tggaccaac ttcgcaaaa tttggaaac 
      ccaactccc gaccaaaac gacaagttg ttgaaaact 
      gtctggaaa ccagttaca aaacctgaa atcgatgtt 
      cttgacatt gggaccgac ctgaaaatg gtctcgaat 
      ccataccgg gagagaata caattttgg gaaaagctt 
      tattccgaa agcccggcc ggcagaaat aagtaa
    mRNA sequence
    • >TC015229 RA
      agaatgttt atttaatgg attgttatg taagcacta 
      tttacagtg tgatcagca ctttagcta cagtaggtt 
      gtttggtgt aacaatgga gttatcaat agaacaggg 
      gaggcttcg agggtgtgt cgggactga catcaaagg 
      gagcaattt tctcaaatt caagggaat cccatatgc 
      gaggccccc gctgggtga cctccgatt caaggcgcc 
      ccaacctcc ggaaaaatg ggctggtgt tttagacgc 
      gtccaaaga aggggatgc ttcctacca ctgcgacgt 
      agtcagaga gaatatcat ggttgggag cgaaaactg 
      tctggtctt gaacgtgta cacgcgaaa actgggtga 
      tggtaaaaa tttgcgccc agtcatggt ttggatcca 
      cgggggtgg ttttgtttg gggctccgg gtctgaaga 
      actatacgg ccctgattt cctaatgac cgaagatat 
      cgtctacgt taccataaa ttatcggtt gggaatgtt 
      gggtttttt gaactttga ggacccgtc cttgggggt 
      gcctggcaa tgccgggtt caaggacat gataatggg 
      tttaaaatg ggtccagag gaatatttc ggctttcgg 
      gggtgaccc caacaatgt tacaatttt cggggagag 
      tgccggggg tgcgtctgt gcatttgct aacactgtc 
      acctctggc aaaaggtct ttttcacaa agccattgt 
      tcagagtgc ctgtgcatt gaacccggg ggctagagg 
      cacgaaagg ggcgcgtca tattgccaa ggctttgaa 
      ccttgaagg ggccgatga taagacagt tttggagca 
      tttgaagac taaaacttt cgccgagat ttttgaaat 
      tcagaaaaa aattgttga tcctctggt ggctaatga 
      gattcgacc gtttgggcc tgtggttga atctggtga 
      caaaaaagc ggctttttt gacacaaga accgtacga 
      aattatatt atcagggaa tttcaatca agttcccgt 
      tatgattgg gtttacgtc gcgtgaagg aatgttggc 
      ccaaatgtt taaagattg tcgtaagga cttttctgt 
      tcaggatca cattccttg gacgtttgg gtatagtaa 
      ggaggaagc gaaagctgt taccactac agtcaaaaa 
      attctactt tggtaatga ggagccatc agcggataa 
      tgttgtaaa agcatttga tttggcaag cgacgtttt 
      ctttcttta tggtattca caaagctgt tggggctca 
      atacagtcg gtctaaagc accaatcta cgtatacga 
      aatgacaat cgaaacaga actgaactt tttcaagaa 
      cctactaaa aattacaga acctggtgt ttgtcacgc 
      tgacgacat tggttatct cttcaatca ttttgtaac 
      tccgaaaat aactcccgg aagcgccgc agatatcgg 
      tcgttcaag gttcgtcaa gttgtggac caacttcgc 
      aaaatttgg aaacccaac tcccgacca aaacgacaa 
      gttgttgaa aactgtctg gaaaccagt tacaaaacc 
      tgaaatcga tgttcttga cattgggac cgacctgaa 
      aatggtctc gaatccata ccgggagag aatacaatt 
      ttgggaaaa gctttattc cgaaagccc ggccggcag 
      aaataagta aacagattt tttgttaat aaaaattgt 
      gatacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY