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Gene information
General information
TC015224 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7024848..7026176
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015224
    iBeetle screen iB_02435

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015224 RA
      MKLAAVLSL VILTQGEEF SQANYLFTS DVYKECVKN 
      EGGNLLVSP LSVEIVLAL AQCGARDET AREIQTALH 
      LASSDQKTA FKSVISGLK GGEHYSLHT ANKIYLHDN 
      FSIRDDFKT IATEMFQSE VENIDFSKT EAAGIINKW 
      VEDQTNQKI KNLVRPESL QNSPSVLVN ALYFKARWA 
      SKNFLKYPY LTKIFSKTA TDQIESEFM IDIGGSYQL 
      HEDKELGAR FLKAPFKGQ DAHMVFVLP DAVDGLGRL 
      EEQADKIFR EHSFQPVII NVELPRFKI ESTVNFKSI 
      LQKLGVRKA FMNGEADFS GIAGKKGEL VVGQVTQKS 
      YIDVNKDGV EAAAATNLV FTLSGPPRT TKEFKANHP 
      FLFYIQAKG VVLFAGRVV DPKY
    CDS sequence
    • >TC015224 RA
      atgaagctc gctgcggtt ctctcactc gtgattttg 
      acccaagga gaagagttt tcgcaagct aattatttg 
      ttcacttct gatgtttac aaagagtgt gttaaaaat 
      gagggcggt aatttacta gtgagtccc ctttccgtg 
      gaaatagtc ctagcattg gcccagtgt ggtgccaga 
      gatgagaca gcacgagaa atccaaacc gctttacat 
      ttggccagt tctgaccaa aaaactgct ttcaaaagt 
      gttatttcg ggccttaag gggggcgaa cactactcg 
      cttcacaca gccaacaag atttatctt cacgataat 
      ttctcgatt agggacgat tttaagaca attgctaca 
      gaaatgttc caatcagag gttgaaaat atagatttt 
      tcgaaaact gaggcagct gggattata aataagtgg 
      gtcgaggac caaacgaac caaaaaata aaaaacttg 
      gtgcgtccc gaaagtctc caaaacagc ccttctgtc 
      ctggtcaat gcgctttat ttcaaggcg cgttgggcc 
      tctaaaaac ttccttaaa tacccctac ttaactaag 
      attttttcc aaaacggcc actgaccag attgaaagt 
      gaattcatg atagatata ggcggttct tatcaactg 
      cacgaagat aaagaactg ggcgctcgg ttcctgaaa 
      gctccgttt aaaggacaa gacgcccat atggttttc 
      gtgctacca gacgctgtg gacgggctt ggaagactt 
      gaggaacaa gcggataaa attttcagg gaacattcg 
      ttccagccg gttattata aacgtggag ttaccacgg 
      ttcaaaatt gagagtacc gttaatttc aagtcgata 
      ttgcaaaag ttaggtgtt cggaaagct tttatgaac 
      ggcgaggcc gattttagc ggaattgct ggaaaaaag 
      ggggagctt gtcgtgggg caggttaca caaaagagt 
      tacattgat gttaacaag gatggggtg gaagcagca 
      gctgccacg aatttagtg tttacgctg tctgggccg 
      ccacgaact acgaaagaa tttaaagca aatcatcca 
      tttttgttt tacattcaa gccaaaggc gtggtgctg 
      tttgcagga agagttgtt gatccgaaa tattaa
    mRNA sequence
    • >TC015224 RA
      caaagttac aatgaagct cgctgcggt tctctcact 
      cgtgatttt gacccaagg agaagagtt ttcgcaagc 
      taattattt gttcacttc tgatgttta caaagagtg 
      tgttaaaaa tgagggcgg taatttact agtgagtcc 
      cctttccgt ggaaatagt cctagcatt ggcccagtg 
      tggtgccag agatgagac agcacgaga aatccaaac 
      cgctttaca tttggccag ttctgacca aaaaactgc 
      tttcaaaag tgttatttc gggccttaa ggggggcga 
      acactactc gcttcacac agccaacaa gatttatct 
      tcacgataa tttctcgat tagggacga ttttaagac 
      aattgctac agaaatgtt ccaatcaga ggttgaaaa 
      tatagattt ttcgaaaac tgaggcagc tgggattat 
      aaataagtg ggtcgagga ccaaacgaa ccaaaaaat 
      aaaaaactt ggtgcgtcc cgaaagtct ccaaaacag 
      cccttctgt cctggtcaa tgcgcttta tttcaaggc 
      gcgttgggc ctctaaaaa cttccttaa ataccccta 
      cttaactaa gattttttc caaaacggc cactgacca 
      gattgaaag tgaattcat gatagatat aggcggttc 
      ttatcaact gcacgaaga taaagaact gggcgctcg 
      gttcctgaa agctccgtt taaaggaca agacgccca 
      tatggtttt cgtgctacc agacgctgt ggacgggct 
      tggaagact tgaggaaca agcggataa aattttcag 
      ggaacattc gttccagcc ggttattat aaacgtgga 
      gttaccacg gttcaaaat tgagagtac cgttaattt 
      caagtcgat attgcaaaa gttaggtgt tcggaaagc 
      ttttatgaa cggcgaggc cgattttag cggaattgc 
      tggaaaaaa gggggagct tgtcgtggg gcaggttac 
      acaaaagag ttacattga tgttaacaa ggatggggt 
      ggaagcagc agctgccac gaatttagt gtttacgct 
      gtctgggcc gccacgaac tacgaaaga atttaaagc 
      aaatcatcc atttttgtt ttacattca agccaaagg 
      cgtggtgct gtttgcagg aagagttgt tgatccgaa 
      atattaaac actgtagtt tggtttcaa taaaagatt 
      gaacaggaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY