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Gene information
General information
TC015220 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid55: 55081..58898
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015220
    iBeetle screen iB_02433
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015220 RA
      MAYNTRRSG GGGEPINTV GARRNPPLT NNFIPGGQL 
      APQNRPPQP RMQPPPNQN NANFRPQQR MPVPPQGQK 
      QMPPNDPPQ KMPQQIPGL DIPLKVEGA MQSDAANET 
      GKQKPLWLK TAANKITRK ERQRRRNIR LSRMIQPKN 
      AVMILNELV KSATYTVTD VPQPPEMNQ YTATVLVDG 
      VTHIGHGRS KMEAKSMAA ENALKYIVK NRQFSALSK 
      DVEGDDKME MGEDGTPTL PWQHVASFA LYKLLNAWG 
      EDPNQTRGD AGPGQIPQN RPSRKMPEN AAHMNPLML 
      LNQMMPHAQ FEELGRTGN PPNVIFTFK CTVGGQSFN 
      GTGPNKKSA KKMAAFAVC HKMLGIQYP QEVYTPSN
    CDS sequence
    • >TC015220 RA
      atggcttac aacacccga agaagtggc ggcggcggc 
      gagcccata aatacagtt ggtgcacgt cggaatcct 
      cccctcacc aacaatttc atacctggc ggccagcta 
      gcgccccag aacaggcct ccgcagccc agaatgcag 
      ccgccaccc aatcagaat aacgccaat ttcaggccg 
      caacagagg atgccagtg ccccctcaa ggacaaaag 
      caaatgcct cccaatgac ccgccacag aagatgccg 
      cagcaaata ccggggtta gatatacca ctgaaagtg 
      gaaggagca atgcagtct gatgctgcc aatgaaacg 
      ggaaagcag aaacctctg tggctaaag acagcggcc 
      aataaaatt acgagaaaa gagcgccag cggcgccga 
      aacatccgt ctcagtcga atgattcag ccgaaaaat 
      gccgtcatg atcttgaat gaactcgtc aaaagtgca 
      acatatact gtaacagac gtgcctcaa ccccctgag 
      atgaaccag tacacggca acagttctg gtcgatgga 
      gtaacccat atcggtcat ggccgtagc aaaatggaa 
      gccaaaagc atggcggct gaaaacgcc cttaaatac 
      attgtgaag aatcgccag ttctccgct ttatcaaaa 
      gacgtagag ggggacgat aaaatggaa atgggtgag 
      gatggaacg ccgactctt ccatggcaa cacgtcgca 
      tcctttgct ttgtataag ttgctcaat gcgtggggc 
      gaagatccc aatcagacg agaggggac gcgggaccg 
      ggacagatt ccgcaaaat agaccttcg cgaaaaatg 
      cccgaaaat gcagcgcat atgaacccg ctaatgctt 
      ctgaatcag atgatgccg catgcgcag tttgaggaa 
      ctggggagg accgggaat cccccgaat gtcattttc 
      acgttcaaa tgtacggtc ggtggacag tcgttcaat 
      ggaacaggt cccaataag aagtctgcg aagaaaatg 
      gctgctttc gcagtgtgt cataagatg ttaggtatc 
      caatacccg caagaagta tacactcct tcaaattaa
    mRNA sequence
    • >TC015220 RA
      actgtttcc ggtcgccat cacaccttc cattttatc 
      gtgacgcct tcatgcttc attttaact tttcgaaaa 
      aaagtttat ttcttttga aaattatcc ggttttttg 
      tgaatcttt cgaaatatt gaacgcaat tataaaaaa 
      ccagctata tctccgata ctttaatta aattaacga 
      tggcttaca acacccgaa gaagtggcg gcggcggcg 
      agcccataa atacagttg gtgcacgtc ggaatcctc 
      ccctcacca acaatttca tacctggcg gccagctag 
      cgccccaga acaggcctc cgcagccca gaatgcagc 
      cgccaccca atcagaata acgccaatt tcaggccgc 
      aacagagga tgccagtgc cccctcaag gacaaaagc 
      aaatgcctc ccaatgacc cgccacaga agatgccgc 
      agcaaatac cggggttag atataccac tgaaagtgg 
      aaggagcaa tgcagtctg atgctgcca atgaaacgg 
      gaaagcaga aacctctgt ggctaaaga cagcggcca 
      ataaaatta cgagaaaag agcgccagc ggcgccgaa 
      acatccgtc tcagtcgaa tgattcagc cgaaaaatg 
      ccgtcatga tcttgaatg aactcgtca aaagtgcaa 
      catatactg taacagacg tgcctcaac cccctgaga 
      tgaaccagt acacggcaa cagttctgg tcgatggag 
      taacccata tcggtcatg gccgtagca aaatggaag 
      ccaaaagca tggcggctg aaaacgccc ttaaataca 
      ttgtgaaga atcgccagt tctccgctt tatcaaaag 
      acgtagagg gggacgata aaatggaaa tgggtgagg 
      atggaacgc cgactcttc catggcaac acgtcgcat 
      cctttgctt tgtataagt tgctcaatg cgtggggcg 
      aagatccca atcagacga gaggggacg cgggaccgg 
      gacagattc cgcaaaata gaccttcgc gaaaaatgc 
      ccgaaaatg cagcgcata tgaacccgc taatgcttc 
      tgaatcaga tgatgccgc atgcgcagt ttgaggaac 
      tggggagga ccgggaatc ccccgaatg tcattttca 
      cgttcaaat gtacggtcg gtggacagt cgttcaatg 
      gaacaggtc ccaataaga agtctgcga agaaaatgg 
      ctgctttcg cagtgtgtc ataagatgt taggtatcc 
      aatacccgc aagaagtat acactcctt caaattaat 
      taaattgaa gaatttttc tcaaatttt tgcattctg 
      aagtgtaac ctgatgtat tcattgtat tgcttctag 
      atttggcta agtgcaaat aaagtgcgt ttcttgag