GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02432 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)ovary without detectable defects - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC015218 RA
MFWILVGLA LVALYWALS RPHSYWKRK GVVQGSPVF LLGDSWPML FRKYSPPEM TELFYKASP NTRYFGIYE FLTPGLLLR DPDLIKQIT VKDFDHFVD RRPFVPEKA DPLFGKSLF SLKGPKWRA MRSTLSPVF TSSKMKYMF QLMSQTGER FINHFLNQK EEVVTVEMK EITTKFAND VIGNIVFGY ECDSVKDPK NEFYVMGAD LTNFRSLRF LLVFSANMI VPKLLALFK IPLFPKKVS DFFVRVVND NIESREKHG IVRPDMIHL LLKMKSNNL KHEEVETVP DAGFASVEE SELGKNAKT VTKITNEDI AAQALLFFF AGFDSVSSL ISYMAYELA VNPDVQTKL LQEVDETRQ KSDGKITYE ALMSMKYMD MVVSEALRK WPNAIATER VCTKPYTIE PKLPDEKPL RLEIGDVVA IPMYAIQRD PKYFPEPER FIPERFSDE NKSKVQPYT FMSFGTGPR SCIGSRFAL LETKLLFYY FLTNFEFVP VEKTQIPIK FNRKALNMS PENGFWLGL KRRV
- >TC015218 RA
atgttttgg atactggtt gggttagca ctagttgcc ctttactgg gctctctcc cgcccccac agctactgg aaaagaaag ggggtggtc cagggaagt ccggttttt cttcttgga gacagctgg cccatgctt ttccgaaaa tactcaccg ccggaaatg acagaactt ttttataaa gccagtcca aatacaaga tacttcgga atttacgag tttctaacc cctggttta ctactaaga gaccccgac ctgatcaag caaatcaca gtcaaagat ttcgaccat tttgtcgat cgacgaccg tttgtgcca gaaaaggct gatccccta tttggcaaa agcttattt tctctaaaa gggccaaaa tggcgtgcg atgcgctcc acacttagc ccagttttc acaagcagc aaaatgaaa tatatgttc caactgatg tcccaaacc ggtgaaaga ttcatcaat cacttccta aaccaaaaa gaagaagtt gtgaccgtt gaaatgaag gaaatcacc acgaaattc gccaatgac gtaatcgga aacatcgtt ttcggctac gaatgcgac tctgtcaaa gacccgaag aatgaattt tacgtaatg ggggccgat ctaactaat ttccgttct ttgcgattt ctgctagtc ttcagcgca aacatgatt gtaccaaaa ctattagct ttgtttaaa atcccattg ttcccgaaa aaagtctcc gacttcttc gtaagagtg gtgaatgat aatatagaa agtcgcgaa aaacacgga attgtgaga cctgacatg atccaccta ctgctaaaa atgaagagc aataatttg aaacacgag gaagtggag actgttccc gacgctggt tttgcctca gtcgaagaa tccgaactt ggcaaaaac gccaaaaca gttaccaaa ataacaaac gaggacatt gctgcccaa gccctcctg ttcttcttt gccggcttc gacagcgtt tcttcgctc atttcttac atggcatat gaactagca gtgaaccca gacgtgcaa actaaactg ctacaagaa gttgacgaa acgaggcaa aaaagtgat ggaaaaatc acctacgag gctttaatg tccatgaaa tacatggac atggtcgtg tcagaagcg ttgcgaaaa tggcccaac gccatcgcc accgaacgc gtctgcact aaaccatac acaattgag ccgaaattg cccgatgag aagcctctc cggttggaa atcggagat gtggtggcg atcccgatg tacgccatc cagagggac cctaaatat tttccagaa cccgagcgg tttattcca gaacggttt agtgacgag aataaatca aaggttcaa ccgtacacg tttatgtcg tttgggacg ggaccaagg agctgcatc ggctccaga tttgcgctc cttgaaact aaactcctg ttttattat tttctgacg aattttgag ttcgttccc gttgagaaa acgcaaata ccgatcaaa ttcaaccgg aaggcgctg aatatgtcg cccgaaaat ggcttttgg ttgggcctc aaacggcgt gtgtaa
- >TC015218 RA
caagctggt gcaaatact aacacaatt cgttatagg ctgcccaat ttatagcaa aattttatc tagtggttt tatcttgag tgcagaaga gcgatttta caccttagt ggcttctaa acctgagtg aagcactta gcgaatact tggattatc ttttccacg atcaaaaca attgattag accagggta taaatagaa cgcaaaccg tcgcagctt tcatacagt gctcgtgac acgagtagc tactgaagg taaaaatcc ttcagttta aaaaaatcc aagaaagtg ttgtttttc cgtttagtc aacaaaatg ttttggata ctggttggg ttagcacta gttgccctt tactgggct ctctcccgc ccccacagc tactggaaa agaaagggg gtggtccag ggaagtccg gtttttctt cttggagac agctggccc atgcttttc cgaaaatac tcaccgccg gaaatgaca gaacttttt tataaagcc agtccaaat acaagatac ttcggaatt tacgagttt ctaacccct ggtttacta ctaagagac cccgacctg atcaagcaa atcacagtc aaagatttc gaccatttt gtcgatcga cgaccgttt gtgccagaa aaggctgat cccctattt ggcaaaagc ttattttct ctaaaaggg ccaaaatgg cgtgcgatg cgctccaca cttagccca gttttcaca agcagcaaa atgaaatat atgttccaa ctgatgtcc caaaccggt gaaagattc atcaatcac ttcctaaac caaaaagaa gaagttgtg accgttgaa atgaaggaa atcaccacg aaattcgcc aatgacgta atcggaaac atcgttttc ggctacgaa tgcgactct gtcaaagac ccgaagaat gaattttac gtaatgggg gccgatcta actaatttc cgttctttg cgatttctg ctagtcttc agcgcaaac atgattgta ccaaaacta ttagctttg tttaaaatc ccattgttc ccgaaaaaa gtctccgac ttcttcgta agagtggtg aatgataat atagaaagt cgcgaaaaa cacggaatt gtgagacct gacatgatc cacctactg ctaaaaatg aagagcaat aatttgaaa cacgaggaa gtggagact gttcccgac gctggtttt gcctcagtc gaagaatcc gaacttggc aaaaacgcc aaaacagtt accaaaata acaaacgag gacattgct gcccaagcc ctcctgttc ttctttgcc ggcttcgac agcgtttct tcgctcatt tcttacatg gcatatgaa ctagcagtg aacccagac gtgcaaact aaactgcta caagaagtt gacgaaacg aggcaaaaa agtgatgga aaaatcacc tacgaggct ttaatgtcc atgaaatac atggacatg gtcgtgtca gaagcgttg cgaaaatgg cccaacgcc atcgccacc gaacgcgtc tgcactaaa ccatacaca attgagccg aaattgccc gatgagaag cctctccgg ttggaaatc ggagatgtg gtggcgatc ccgatgtac gccatccag agggaccct aaatatttt ccagaaccc gagcggttt attccagaa cggtttagt gacgagaat aaatcaaag gttcaaccg tacacgttt atgtcgttt gggacggga ccaaggagc tgcatcggc tccagattt gcgctcctt gaaactaaa ctcctgttt tattatttt ctgacgaat tttgagttc gttcccgtt gagaaaacg caaataccg atcaaattc aaccggaag gcgctgaat atgtcgccc gaaaatggc ttttggttg ggcctcaaa cggcgtgtg taatataat catcttagg tattagtga taaggataa tgtatcgat atttttgta cgaaaataa ataaatcaa ataaatatc caaccaata t
