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Gene information
General information
TC015217 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1291886..1295522
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015217
    iBeetle screen iB_02431

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015217 RA
      MANAILNTF LRKLGPLSA PKLVLNNGA ALLHTSGNK 
      KAKWYPDPE YMAQFDGPV MYPDEVTSK WVLPPWNAQ 
      KAPVERSVK NLTLNFGPQ HPAAHGVLR LVIELDGEM 
      VRRADPHIG LLHRGTEKL IEYKTYTQA LPYFDRLDY 
      VSMMCNEQC YSLAVEKLL NIDVPLRAK YIRTLFAEI 
      TRILNHIMA VGTHALDVG ALTPFFWLF EEREKMMEF 
      YERVSGARM HAAYIRPGG VSLDMPLGL MDDIYEFAS 
      KFGERLDEV EDVLTTNRI WVQRTQDIG VVSAEDALN 
      LGFSGVMLR GSGIKWDLR KVQPYDAYD LVDFDVPIG 
      VKGDCYDRY LCRVEEMRQ SLRIIDQCL NNMPPGEIK 
      TDDAKLTPP SRAEMKTSM EALIHHFKL FTQGYQVPP 
      GATYTAIEA PKGEFGVYL VSDGGSKPY RCKIKAPGF 
      AHLAALEKV GKNHMLADI VAIIGTLDV VFGEIDR
    CDS sequence
    • >TC015217 RA
      atggcaaac gcaatttta aacacgttt ttacgcaaa 
      ctggggccc ctttcagcc cccaaactg gtcctaaac 
      aacggcgct gcgctactg cacaccagt ggcaataaa 
      aaagcgaaa tggtacccc gaccccgaa tacatggcg 
      caattcgac ggcccggtc atgtacccc gacgaggtc 
      accagcaaa tgggtgcta cccccatgg aacgcccag 
      aaagccccc gtcgaacgg tcggtcaaa aacctcact 
      ttgaacttt gggccccag catccggcc gcccacggg 
      gtcctgcgg ctggtgatc gagttggac ggggagatg 
      gtgcggcgg gcggacccc cacataggg ctcctccac 
      cgcgggacg gagaaacta atcgagtac aagacctac 
      acgcaggct ttgccctac tttgaccgc cttgactac 
      gtctcgatg atgtgcaac gagcagtgt tacagtctc 
      gctgttgaa aaactccta aatattgat gtgcccctt 
      agggccaaa tacataaga acgctgttt gcggaaatt 
      acgcgcatt ctgaaccat attatggcc gtggggacg 
      cacgctctg gacgtgggg gccctgact ccgttcttt 
      tggctgttt gaggagcgt gagaaaatg atggagttt 
      tacgagagg gtgtcgggg gcccgcatg cacgcggcg 
      tatatcagg ccagggggc gtttctctg gatatgccg 
      ctaggtctc atggacgat atttacgaa tttgcgtcg 
      aaatttggc gaaagactt gacgaagtt gaggatgtt 
      ctcactacg aataggatt tgggtgcaa aggacgcaa 
      gatattggg gttgtttcg gccgaagat gccctcaat 
      cttgggttc agtggggtc atgttacgg ggctccgga 
      ataaagtgg gatttgcgc aaagttcag ccctacgac 
      gcgtacgat ttggtcgat ttcgacgtc ccaattggg 
      gtaaaaggc gattgttac gatagatac ctgtgtcgc 
      gttgaagaa atgagacag tcgctccga atcatcgac 
      caatgtctt aacaacatg ccaccgggt gaaataaag 
      acagacgac gccaaactc acacctccc agccgagct 
      gaaatgaag acgtcaatg gaagccctc atccaccac 
      ttcaagctg ttcacgcag ggctaccag gtgcccccc 
      ggcgccacc tacaccgca atcgaggcc ccgaagggc 
      gagttcggg gtttactta gtctcagac gggggctcc 
      aagccctac cgctgcaaa atcaaggcg cccggcttc 
      gcccatttg gcggccctg gaaaaagtg gggaaaaac 
      cacatgctg gccgacatt gtggccatt atcgggacc 
      ctcgatgtg gtcttcggc gaaatcgat cgataa
    mRNA sequence
    • >TC015217 RA
      tggccgtta ctttggcca cttccagtc cgggggctg 
      tccgctgtc aagtgtcaa aatggcaaa cgcaatttt 
      aaacacgtt tttacgcaa actggggcc cctttcagc 
      ccccaaact ggtcctaaa caacggcgc tgcgctact 
      gcacaccag tggcaataa aaaagcgaa atggtaccc 
      cgaccccga atacatggc gcaattcga cggcccggt 
      catgtaccc cgacgaggt caccagcaa atgggtgct 
      acccccatg gaacgccca gaaagcccc cgtcgaacg 
      gtcggtcaa aaacctcac tttgaactt tgggcccca 
      gcatccggc cgcccacgg ggtcctgcg gctggtgat 
      cgagttgga cggggagat ggtgcggcg ggcggaccc 
      ccacatagg gctcctcca ccgcgggac ggagaaact 
      aatcgagta caagaccta cacgcaggc tttgcccta 
      ctttgaccg ccttgacta cgtctcgat gatgtgcaa 
      cgagcagtg ttacagtct cgctgttga aaaactcct 
      aaatattga tgtgcccct tagggccaa atacataag 
      aacgctgtt tgcggaaat tacgcgcat tctgaacca 
      tattatggc cgtggggac gcacgctct ggacgtggg 
      ggccctgac tccgttctt ttggctgtt tgaggagcg 
      tgagaaaat gatggagtt ttacgagag ggtgtcggg 
      ggcccgcat gcacgcggc gtatatcag gccaggggg 
      cgtttctct ggatatgcc gctaggtct catggacga 
      tatttacga atttgcgtc gaaatttgg cgaaagact 
      tgacgaagt tgaggatgt tctcactac gaataggat 
      ttgggtgca aaggacgca agatattgg ggttgtttc 
      ggccgaaga tgccctcaa tcttgggtt cagtggggt 
      catgttacg gggctccgg aataaagtg ggatttgcg 
      caaagttca gccctacga cgcgtacga tttggtcga 
      tttcgacgt cccaattgg ggtaaaagg cgattgtta 
      cgatagata cctgtgtcg cgttgaaga aatgagaca 
      gtcgctccg aatcatcga ccaatgtct taacaacat 
      gccaccggg tgaaataaa gacagacga cgccaaact 
      cacacctcc cagccgagc tgaaatgaa gacgtcaat 
      ggaagccct catccacca cttcaagct gttcacgca 
      gggctacca ggtgccccc cggcgccac ctacaccgc 
      aatcgaggc cccgaaggg cgagttcgg ggtttactt 
      agtctcaga cgggggctc caagcccta ccgctgcaa 
      aatcaaggc gcccggctt cgcccattt ggcggccct 
      ggaaaaagt ggggaaaaa ccacatgct ggccgacat 
      tgtggccat tatcgggac cctcgatgt ggtcttcgg 
      cgaaatcga tcgataatt ttaagtgta aataaataa 
      tgaatcaat aaataggtt taaataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY