Status
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Gene information
General information
TC034722 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1369561..1380898
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034722
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02430

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034722 RA
      MEEFRNEIV SRIRSCLIS TKGQVTLRQ LEDDYRTLL 
      GERIPYAKL GHKTLESFI ISIPTIITS RSPSGEILV 
      DAQVSEKTA HISSMVRKQ KSVPKKHVR IAPKLNRAM 
      AQPPPNAAK WRPKQKPLM RKTYGNTPK LAAVSHNQF 
      VNNYSGYGK KTVPSKVVV VERKEEVKR NNSVEVQRN 
      NNGLEEVKN NSRLEKDEE NSMFSSTLK RITQVMKKV 
      NVETDSGTS SPTTEYAAG YKLSSDFLK TGDPISDLR 
      NFVAYHKLG KVDVKFTET KLKKSKVPQ CHCKVTVGQ 
      HKYSSYPED FYDRDAAER HASQKALDD LMQKYSRRR 
      SLLLSSNDD IIERIPPML EKHNNAVWM WQIEADYRD 
      RFNEQLPPD WLQVIDNSP FVSIEKCHG GCVLKHCNP 
      DDKGKKLDI SLNVGDVSV PCNTVDFGD SNRLYAVVT 
      VAHSVNEIW CQHCGTPEY EKFVEMTQN MESYYESYK 
      TELKAKMIN AGSCYVIQN EGLWIRVRV LKTPENGYV 
      DCFLIDYGE ELSISIDNV YLLKRQFAT EQAQAFVCR 
      LDGLEDFYE ASIDSEILA GLVGKEYVL EIVTDDISD 
      TGDVTIPVV MYDVTSGAS INEELISSL TIESAIPVL 
      EKDSITEVY VSNIEPNGD VYVQVRTVG YFSLMEDLK 
      NLTDNITSK NPSQLSTTT PTKDNSTGK IYLVMCKMT 
      QQWLRATIV DWSPKGDLA QVYFIDQGN AQVVNVTNE 
      KMYELDKLD SVLSQYPGQ AIKVRFMIE KIPSDFVQK 
      AEKLLPKDR PVLLKIISY DNENVAWVE FFKRTTDGV 
      LVFINKSIS VEAELQQNV DVSNNNETN RKRLLNLVQ 
      FNEASKNVP SGGSLRKPD LPKMGDYFN VNIPFAVNP 
      WNFFVQPLD SFARLKALM NEMQEHYKD THFSPMPLE 
      EIIPGKIYA SKHEDGQWY RTNVLKVIH EGSISVFYC 
      DFGYYTNLT LDQLVPLDA KYMGLPYQA LKAKISGIK 
      PIKNKWTME DCESFKDLI LKKQFVGVI TNIDRDEFH 
      KSDLILEVL LIDTSSEED VNIKEVLIQ KGIATSV
    • >TC034722 RB
      MEEFRNEIV SRIRSCLIS TKGQVTLRQ LEDDYRTLL 
      GERIPYAKL GHKTLESFI ISIPTIITS RSPSGEILV 
      DAQVSEKTA HISSMVRKQ KSVPKKHVR IAPKLNRAM 
      AQPPPNAAK WRPKQKPLM RKTYGNTPK LAAVSHNQF 
      VNNYSGYGK KTVPSKVVV VERKEEVKR NNSVEVQRN 
      NNGLEEVKN NSRLEKDEE NSMFSSTLK RITQVMKKV 
      NVETDSGTS SPTTEYAAG YKLSSDFLK TGDPISDLR 
      NFVAYHKLG KVDVKFTET KLKKSKVPQ CHCKVTVGQ 
      HKYSSYPED FYDRDAAER HASQKALDD LMQKYSRRR 
      SLLLSSNDD IIERIPPML EKHNNAVWM WQIEADYRD 
      RFNEQLPPD WLQVIDNSP FVSIEKCHG GCVLKHCNP 
      DDVLQKGKK LDISLNVGD VSVPCNTVD FGDSNRLYA 
      VVTVAHSVN EIWCQHCGT PEYEKFVEM TQNMESYYE 
      SYKTELKAK MINAGSCYV IQNEGLWIR VRVLKTPEN 
      GYVDCFLID YGEELSISI DNVYLLKRQ FATEQAQAF 
      VCRLDGLED FYEASIDSE ILAGLVGKE YVLEIVTDD 
      ISDTGDVTI PVVMYDVTS GASINEELI SSLTIESAI 
      PVLEKDSIT EVYVSNIEP NGDVYVQVR TVGYFSLME 
      DLKNLTDNI TSKNPSQLS TTTPTKDNS TGKIYLVMC 
      KMTQQWLRA TIVDWSPKG DLAQVYFID QGNAQVVNV 
      TNEKMYELD KLDSVLSQY PGQAIKVRF MIEKIPSDF 
      VQKAEKLLP KDRPVLLKI ISYDNENVA WVEFFKRTT 
      DGVLVFINK SISVEAELQ QNVDVSNNN ETNRKRLLN 
      LVQFNEASK NVPSGGSLR KPDLPKMGD YFNVNIPFA 
      VNPWNFFVQ PLDSFARLK ALMNEMQEH YKDTHFSPM 
      PLEEIIPGK IYASKHEDG QWYRTNVLK VIHEGSISV 
      FYCDFGYYT NLTLDQLVP LDAKYMGLP YQALKAKIS 
      GIKPIKNKW TMEDCESFK DLILKKQFV GVITNIDRD 
      EFHKSDLIL EVLLIDTSS EEDVNIKEV LIQKGIATS 
      V
    CDS sequence
    • >TC034722 RA
      atggaggag ttcaggaac gagattgtc tccaggata 
      cgctcctgt cttatctcc accaagggg caggtgacc 
      ctccggcag ctggaagat gactaccgc acccttctt 
      ggcgagcga atcccttac gcaaaactg ggccacaaa 
      acgcttgaa agtttcata atatcaatc ccgaccatc 
      attacttca cgtagtccg tcaggtgaa attttagtc 
      gatgctcaa gtttcggaa aaaaccgct cacatttcg 
      agcatggta cgtaaacaa aaatccgta ccgaaaaaa 
      cacgtccgt attgccccg aaactgaat cgagcgatg 
      gcacagccg cctccaaac gccgccaaa tggcgcccc 
      aaacaaaaa ccactaatg cgaaagact tacgggaat 
      acgcctaag ttggcggcg gtttcacac aatcagttt 
      gtcaataat tatagtggg tatggtaag aaaacagtg 
      ccgtcgaag gtggtggtt gtggagagg aaggaagaa 
      gtgaagagg aataattcg gtggaggtg cagaggaat 
      aataacggt ttggaagag gtgaagaat aacagtagg 
      ttggaaaag gatgaggag aattcgatg ttttcgagc 
      actttgaaa cggattacc caggttatg aagaaggtt 
      aatgtggag acggatagt gggactagc agtccgacg 
      accgaatat gcagcaggg tataagtta tcgtccgat 
      tttttgaag actggtgat cccatctct gaccttcgt 
      aattttgtc gcttatcat aaattaggc aaagtcgat 
      gtgaaattc actgaaacg aaattgaaa aagtcgaaa 
      gtgccacaa tgtcactgc aaagtcacg gtgggtcaa 
      cataaatat tcaagttat ccggaagat ttctacgat 
      cgagacgca gccgaacga catgcctct cagaaagca 
      cttgacgat cttatgcag aaatatagt cgtagaaga 
      tcgttgttg ctttcgagt aatgatgat attattgaa 
      cgaattccc ccaatgttg gagaaacat aataatgca 
      gtgtggatg tggcaaatc gaagccgat tatcgagat 
      cggttcaac gagcaattg ccacctgat tggctacaa 
      gtcatcgat aatagccct tttgtttcg attgaaaaa 
      tgtcacggt ggctgcgtt ctcaagcat tgcaatcct 
      gatgataaa ggcaaaaaa ctcgacata agtttaaac 
      gttggcgat gtttccgtc ccttgtaac actgtcgat 
      tttggtgac agtaatcgt ttgtatgcc gttgttacg 
      gttgcacat tccgtcaac gaaatctgg tgtcagcac 
      tgcggcacg cctgaatat gaaaaattt gtcgaaatg 
      acccaaaat atggagagt tattacgaa agttacaaa 
      accgaactc aaagcaaaa atgatcaat gcgggaagt 
      tgttacgtt attcaaaac gaaggtttg tggataaga 
      gttcgagtg ttaaaaacg ccagaaaat ggttacgtt 
      gattgtttt ttgatcgat tatggtgaa gaacttagc 
      atctcaatc gataatgtt tatttattg aaacgacag 
      tttgctacc gaacaagca caagctttt gtctgtcga 
      ttagacggc ttggaggac ttttacgag gcatccatc 
      gattccgaa attttggca ggtttggta ggaaaggaa 
      tacgttcta gagatcgtc accgatgat ataagtgac 
      accggtgac gtcacaatt cccgttgtc atgtatgac 
      gtcacatcc ggtgcatca atcaacgaa gagttgatt 
      tcatcactg acgatcgaa tcggcgatt ccggtcctg 
      gaaaaagac tcaataacc gaagtttac gtttcaaat 
      attgaacca aacggtgac gtgtatgtc caggtccgt 
      actgtcggt tatttcagt ttgatggaa gatttgaag 
      aatttaacc gataatata acgtctaaa aacccctcc 
      cagctttct actaccaca ccaaccaaa gacaacagc 
      accggtaaa atataccta gtgatgtgt aaaatgacg 
      caacaatgg ctcagagca acgattgtt gattggtca 
      ccgaaaggt gacttagca caagtgtat ttcattgat 
      caaggaaat gcccaagtt gtgaatgtg accaatgaa 
      aaaatgtac gaactggat aagttagac agtgtctta 
      agtcagtat ccaggtcag gcgattaaa gttcgcttc 
      atgattgag aaaatccca agtgatttt gtgcaaaaa 
      gccgagaaa ttgctaccg aaagatcgg ccagttttg 
      ctcaaaatt atcagttat gataatgag aatgtggcc 
      tgggttgag tttttcaaa cggacgaca gatggcgta 
      cttgttttc atcaataaa tctatctcg gtcgaagcc 
      gagttgcaa caaaacgtt gacgttagc aataataac 
      gagaccaac cgcaaaaga ctgctcaat ttggtgcaa 
      ttcaacgaa gccagcaaa aatgtccca tctgggggc 
      tctctccga aagcccgat ttgcccaag atgggcgac 
      tatttcaac gtcaatata ccatttgct gtgaatccc 
      tggaatttc tttgtgcaa ccgctggat tcgttcgca 
      cgtctcaag gcgttaatg aacgaaatg caggagcat 
      tacaaggac acccatttt agtccaatg ccacttgaa 
      gagataatt cctggtaaa atttacgcg tcgaaacac 
      gaagatggg caatggtac agaaccaac gtcttgaag 
      gtcatccac gagggttcg atcagtgtt ttttactgc 
      gattttggt tattacacc aacttgaca ctggatcag 
      ttggtacca ctggatgcc aagtacatg ggtttgccc 
      taccaggcg ctcaaggcg aaaatttcc ggaattaag 
      ccgattaaa aacaagtgg acgatggaa gactgcgag 
      agttttaaa gatctgatt ttgaagaaa cagtttgtt 
      ggtgttatt acgaatata gacagggac gagtttcat 
      aaaagtgat cttatcctg gaagtgtta ctgattgac 
      acttccagt gaggaagat gttaatatt aaggaagta 
      ctcatccag aaaggtatt gcaactagt gtgtaa
    • >TC034722 RB
      atggaggag ttcaggaac gagattgtc tccaggata 
      cgctcctgt cttatctcc accaagggg caggtgacc 
      ctccggcag ctggaagat gactaccgc acccttctt 
      ggcgagcga atcccttac gcaaaactg ggccacaaa 
      acgcttgaa agtttcata atatcaatc ccgaccatc 
      attacttca cgtagtccg tcaggtgaa attttagtc 
      gatgctcaa gtttcggaa aaaaccgct cacatttcg 
      agcatggta cgtaaacaa aaatccgta ccgaaaaaa 
      cacgtccgt attgccccg aaactgaat cgagcgatg 
      gcacagccg cctccaaac gccgccaaa tggcgcccc 
      aaacaaaaa ccactaatg cgaaagact tacgggaat 
      acgcctaag ttggcggcg gtttcacac aatcagttt 
      gtcaataat tatagtggg tatggtaag aaaacagtg 
      ccgtcgaag gtggtggtt gtggagagg aaggaagaa 
      gtgaagagg aataattcg gtggaggtg cagaggaat 
      aataacggt ttggaagag gtgaagaat aacagtagg 
      ttggaaaag gatgaggag aattcgatg ttttcgagc 
      actttgaaa cggattacc caggttatg aagaaggtt 
      aatgtggag acggatagt gggactagc agtccgacg 
      accgaatat gcagcaggg tataagtta tcgtccgat 
      tttttgaag actggtgat cccatctct gaccttcgt 
      aattttgtc gcttatcat aaattaggc aaagtcgat 
      gtgaaattc actgaaacg aaattgaaa aagtcgaaa 
      gtgccacaa tgtcactgc aaagtcacg gtgggtcaa 
      cataaatat tcaagttat ccggaagat ttctacgat 
      cgagacgca gccgaacga catgcctct cagaaagca 
      cttgacgat cttatgcag aaatatagt cgtagaaga 
      tcgttgttg ctttcgagt aatgatgat attattgaa 
      cgaattccc ccaatgttg gagaaacat aataatgca 
      gtgtggatg tggcaaatc gaagccgat tatcgagat 
      cggttcaac gagcaattg ccacctgat tggctacaa 
      gtcatcgat aatagccct tttgtttcg attgaaaaa 
      tgtcacggt ggctgcgtt ctcaagcat tgcaatcct 
      gatgatgtt ttacagaaa ggcaaaaaa ctcgacata 
      agtttaaac gttggcgat gtttccgtc ccttgtaac 
      actgtcgat tttggtgac agtaatcgt ttgtatgcc 
      gttgttacg gttgcacat tccgtcaac gaaatctgg 
      tgtcagcac tgcggcacg cctgaatat gaaaaattt 
      gtcgaaatg acccaaaat atggagagt tattacgaa 
      agttacaaa accgaactc aaagcaaaa atgatcaat 
      gcgggaagt tgttacgtt attcaaaac gaaggtttg 
      tggataaga gttcgagtg ttaaaaacg ccagaaaat 
      ggttacgtt gattgtttt ttgatcgat tatggtgaa 
      gaacttagc atctcaatc gataatgtt tatttattg 
      aaacgacag tttgctacc gaacaagca caagctttt 
      gtctgtcga ttagacggc ttggaggac ttttacgag 
      gcatccatc gattccgaa attttggca ggtttggta 
      ggaaaggaa tacgttcta gagatcgtc accgatgat 
      ataagtgac accggtgac gtcacaatt cccgttgtc 
      atgtatgac gtcacatcc ggtgcatca atcaacgaa 
      gagttgatt tcatcactg acgatcgaa tcggcgatt 
      ccggtcctg gaaaaagac tcaataacc gaagtttac 
      gtttcaaat attgaacca aacggtgac gtgtatgtc 
      caggtccgt actgtcggt tatttcagt ttgatggaa 
      gatttgaag aatttaacc gataatata acgtctaaa 
      aacccctcc cagctttct actaccaca ccaaccaaa 
      gacaacagc accggtaaa atataccta gtgatgtgt 
      aaaatgacg caacaatgg ctcagagca acgattgtt 
      gattggtca ccgaaaggt gacttagca caagtgtat 
      ttcattgat caaggaaat gcccaagtt gtgaatgtg 
      accaatgaa aaaatgtac gaactggat aagttagac 
      agtgtctta agtcagtat ccaggtcag gcgattaaa 
      gttcgcttc atgattgag aaaatccca agtgatttt 
      gtgcaaaaa gccgagaaa ttgctaccg aaagatcgg 
      ccagttttg ctcaaaatt atcagttat gataatgag 
      aatgtggcc tgggttgag tttttcaaa cggacgaca 
      gatggcgta cttgttttc atcaataaa tctatctcg 
      gtcgaagcc gagttgcaa caaaacgtt gacgttagc 
      aataataac gagaccaac cgcaaaaga ctgctcaat 
      ttggtgcaa ttcaacgaa gccagcaaa aatgtccca 
      tctgggggc tctctccga aagcccgat ttgcccaag 
      atgggcgac tatttcaac gtcaatata ccatttgct 
      gtgaatccc tggaatttc tttgtgcaa ccgctggat 
      tcgttcgca cgtctcaag gcgttaatg aacgaaatg 
      caggagcat tacaaggac acccatttt agtccaatg 
      ccacttgaa gagataatt cctggtaaa atttacgcg 
      tcgaaacac gaagatggg caatggtac agaaccaac 
      gtcttgaag gtcatccac gagggttcg atcagtgtt 
      ttttactgc gattttggt tattacacc aacttgaca 
      ctggatcag ttggtacca ctggatgcc aagtacatg 
      ggtttgccc taccaggcg ctcaaggcg aaaatttcc 
      ggaattaag ccgattaaa aacaagtgg acgatggaa 
      gactgcgag agttttaaa gatctgatt ttgaagaaa 
      cagtttgtt ggtgttatt acgaatata gacagggac 
      gagtttcat aaaagtgat cttatcctg gaagtgtta 
      ctgattgac acttccagt gaggaagat gttaatatt 
      aaggaagta ctcatccag aaaggtatt gcaactagt 
      gtgtaa
    mRNA sequence
    • >TC034722 RA
      aaaacaaga ctagaaaat tgccatttc tcctggtgc 
      gtttttatc gagtggtta gtcgctggg gcccctttg 
      agcgccccc catcaataa tttggcgcc acctcaatt 
      tgacagttg acattttat caaaaattt tgcaacctg 
      ttccgtttc cataaagtg gggaaaatt gcgtttttg 
      gcaaaactg ggggttgtt tgcggtcgt ggggcgcgc 
      ccttggtgc gcagagtgg ggcgcgagt gccacaagc 
      agttttgga tgaatgtag gttagtgcc agtagtgag 
      ttggacttg gtgctcccg cgatggagg agttcagga 
      acgagattg tctccagga tacgctcct gtcttatct 
      ccaccaagg ggcaggtga ccctccggc agctggaag 
      atgactacc gcacccttc ttggcgagc gaatccctt 
      acgcaaaac tgggccaca aaacgcttg aaagtttca 
      taatatcaa tcccgacca tcattactt cacgtagtc 
      cgtcaggtg aaattttag tcgatgctc aagtttcgg 
      aaaaaaccg ctcacattt cgagcatgg tacgtaaac 
      aaaaatccg taccgaaaa aacacgtcc gtattgccc 
      cgaaactga atcgagcga tggcacagc cgcctccaa 
      acgccgcca aatggcgcc ccaaacaaa aaccactaa 
      tgcgaaaga cttacggga atacgccta agttggcgg 
      cggtttcac acaatcagt ttgtcaata attatagtg 
      ggtatggta agaaaacag tgccgtcga aggtggtgg 
      ttgtggaga ggaaggaag aagtgaaga ggaataatt 
      cggtggagg tgcagagga ataataacg gtttggaag 
      aggtgaaga ataacagta ggttggaaa aggatgagg 
      agaattcga tgttttcga gcactttga aacggatta 
      cccaggtta tgaagaagg ttaatgtgg agacggata 
      gtgggacta gcagtccga cgaccgaat atgcagcag 
      ggtataagt tatcgtccg attttttga agactggtg 
      atcccatct ctgaccttc gtaattttg tcgcttatc 
      ataaattag gcaaagtcg atgtgaaat tcactgaaa 
      cgaaattga aaaagtcga aagtgccac aatgtcact 
      gcaaagtca cggtgggtc aacataaat attcaagtt 
      atccggaag atttctacg atcgagacg cagccgaac 
      gacatgcct ctcagaaag cacttgacg atcttatgc 
      agaaatata gtcgtagaa gatcgttgt tgctttcga 
      gtaatgatg atattattg aacgaattc ccccaatgt 
      tggagaaac ataataatg cagtgtgga tgtggcaaa 
      tcgaagccg attatcgag atcggttca acgagcaat 
      tgccacctg attggctac aagtcatcg ataatagcc 
      cttttgttt cgattgaaa aatgtcacg gtggctgcg 
      ttctcaagc attgcaatc ctgatgata aaggcaaaa 
      aactcgaca taagtttaa acgttggcg atgtttccg 
      tcccttgta acactgtcg attttggtg acagtaatc 
      gtttgtatg ccgttgtta cggttgcac attccgtca 
      acgaaatct ggtgtcagc actgcggca cgcctgaat 
      atgaaaaat ttgtcgaaa tgacccaaa atatggaga 
      gttattacg aaagttaca aaaccgaac tcaaagcaa 
      aaatgatca atgcgggaa gttgttacg ttattcaaa 
      acgaaggtt tgtggataa gagttcgag tgttaaaaa 
      cgccagaaa atggttacg ttgattgtt ttttgatcg 
      attatggtg aagaactta gcatctcaa tcgataatg 
      tttatttat tgaaacgac agtttgcta ccgaacaag 
      cacaagctt ttgtctgtc gattagacg gcttggagg 
      acttttacg aggcatcca tcgattccg aaattttgg 
      caggtttgg taggaaagg aatacgttc tagagatcg 
      tcaccgatg atataagtg acaccggtg acgtcacaa 
      ttcccgttg tcatgtatg acgtcacat ccggtgcat 
      caatcaacg aagagttga tttcatcac tgacgatcg 
      aatcggcga ttccggtcc tggaaaaag actcaataa 
      ccgaagttt acgtttcaa atattgaac caaacggtg 
      acgtgtatg tccaggtcc gtactgtcg gttatttca 
      gtttgatgg aagatttga agaatttaa ccgataata 
      taacgtcta aaaacccct cccagcttt ctactacca 
      caccaacca aagacaaca gcaccggta aaatatacc 
      tagtgatgt gtaaaatga cgcaacaat ggctcagag 
      caacgattg ttgattggt caccgaaag gtgacttag 
      cacaagtgt atttcattg atcaaggaa atgcccaag 
      ttgtgaatg tgaccaatg aaaaaatgt acgaactgg 
      ataagttag acagtgtct taagtcagt atccaggtc 
      aggcgatta aagttcgct tcatgattg agaaaatcc 
      caagtgatt ttgtgcaaa aagccgaga aattgctac 
      cgaaagatc ggccagttt tgctcaaaa ttatcagtt 
      atgataatg agaatgtgg cctgggttg agtttttca 
      aacggacga cagatggcg tacttgttt tcatcaata 
      aatctatct cggtcgaag ccgagttgc aacaaaacg 
      ttgacgtta gcaataata acgagacca accgcaaaa 
      gactgctca atttggtgc aattcaacg aagccagca 
      aaaatgtcc catctgggg gctctctcc gaaagcccg 
      atttgccca agatgggcg actatttca acgtcaata 
      taccatttg ctgtgaatc cctggaatt tctttgtgc 
      aaccgctgg attcgttcg cacgtctca aggcgttaa 
      tgaacgaaa tgcaggagc attacaagg acacccatt 
      ttagtccaa tgccacttg aagagataa ttcctggta 
      aaatttacg cgtcgaaac acgaagatg ggcaatggt 
      acagaacca acgtcttga aggtcatcc acgagggtt 
      cgatcagtg ttttttact gcgattttg gttattaca 
      ccaacttga cactggatc agttggtac cactggatg 
      ccaagtaca tgggtttgc cctaccagg cgctcaagg 
      cgaaaattt ccggaatta agccgatta aaaacaagt 
      ggacgatgg aagactgcg agagtttta aagatctga 
      ttttgaaga aacagtttg ttggtgtta ttacgaata 
      tagacaggg acgagtttc ataaaagtg atcttatcc 
      tggaagtgt tactgattg acacttcca gtgaggaag 
      atgttaata ttaaggaag tactcatcc agaaaggta 
      ttgcaacta gtgtgtaag cttcatttc gaattccta 
      aaaatagta tatttttgt atggttcaa gttttgtgt 
      ttgaattta ttaagaggt atcagtatt gtttctgat 
      agtggttgt tgccataaa ttccaacgt tttaggtta 
      ttgttttgg gccttcacc aaagaatgt ttgttgttt 
      ttttactag atttattgc tgttaatta ccatgttac 
      agctatgaa cacgtttaa taaataccc tttcatttc
    • >TC034722 RB
      aaaacaaga ctagaaaat tgccatttc tcctggtgc 
      gtttttatc gagtggtta gtcgctggg gcccctttg 
      agcgccccc catcaataa tttggcgcc acctcaatt 
      tgacagttg acattttat caaaaattt tgcaacctg 
      ttccgtttc cataaagtg gggaaaatt gcgtttttg 
      gcaaaactg ggggttgtt tgcggtcgt ggggcgcgc 
      ccttggtgc gcagagtgg ggcgcgagt gccacaagc 
      agttttgga tgaatgtag gttagtgcc agtagtgag 
      ttggacttg gtgctcccg cgatggagg agttcagga 
      acgagattg tctccagga tacgctcct gtcttatct 
      ccaccaagg ggcaggtga ccctccggc agctggaag 
      atgactacc gcacccttc ttggcgagc gaatccctt 
      acgcaaaac tgggccaca aaacgcttg aaagtttca 
      taatatcaa tcccgacca tcattactt cacgtagtc 
      cgtcaggtg aaattttag tcgatgctc aagtttcgg 
      aaaaaaccg ctcacattt cgagcatgg tacgtaaac 
      aaaaatccg taccgaaaa aacacgtcc gtattgccc 
      cgaaactga atcgagcga tggcacagc cgcctccaa 
      acgccgcca aatggcgcc ccaaacaaa aaccactaa 
      tgcgaaaga cttacggga atacgccta agttggcgg 
      cggtttcac acaatcagt ttgtcaata attatagtg 
      ggtatggta agaaaacag tgccgtcga aggtggtgg 
      ttgtggaga ggaaggaag aagtgaaga ggaataatt 
      cggtggagg tgcagagga ataataacg gtttggaag 
      aggtgaaga ataacagta ggttggaaa aggatgagg 
      agaattcga tgttttcga gcactttga aacggatta 
      cccaggtta tgaagaagg ttaatgtgg agacggata 
      gtgggacta gcagtccga cgaccgaat atgcagcag 
      ggtataagt tatcgtccg attttttga agactggtg 
      atcccatct ctgaccttc gtaattttg tcgcttatc 
      ataaattag gcaaagtcg atgtgaaat tcactgaaa 
      cgaaattga aaaagtcga aagtgccac aatgtcact 
      gcaaagtca cggtgggtc aacataaat attcaagtt 
      atccggaag atttctacg atcgagacg cagccgaac 
      gacatgcct ctcagaaag cacttgacg atcttatgc 
      agaaatata gtcgtagaa gatcgttgt tgctttcga 
      gtaatgatg atattattg aacgaattc ccccaatgt 
      tggagaaac ataataatg cagtgtgga tgtggcaaa 
      tcgaagccg attatcgag atcggttca acgagcaat 
      tgccacctg attggctac aagtcatcg ataatagcc 
      cttttgttt cgattgaaa aatgtcacg gtggctgcg 
      ttctcaagc attgcaatc ctgatgatg ttttacaga 
      aaggcaaaa aactcgaca taagtttaa acgttggcg 
      atgtttccg tcccttgta acactgtcg attttggtg 
      acagtaatc gtttgtatg ccgttgtta cggttgcac 
      attccgtca acgaaatct ggtgtcagc actgcggca 
      cgcctgaat atgaaaaat ttgtcgaaa tgacccaaa 
      atatggaga gttattacg aaagttaca aaaccgaac 
      tcaaagcaa aaatgatca atgcgggaa gttgttacg 
      ttattcaaa acgaaggtt tgtggataa gagttcgag 
      tgttaaaaa cgccagaaa atggttacg ttgattgtt 
      ttttgatcg attatggtg aagaactta gcatctcaa 
      tcgataatg tttatttat tgaaacgac agtttgcta 
      ccgaacaag cacaagctt ttgtctgtc gattagacg 
      gcttggagg acttttacg aggcatcca tcgattccg 
      aaattttgg caggtttgg taggaaagg aatacgttc 
      tagagatcg tcaccgatg atataagtg acaccggtg 
      acgtcacaa ttcccgttg tcatgtatg acgtcacat 
      ccggtgcat caatcaacg aagagttga tttcatcac 
      tgacgatcg aatcggcga ttccggtcc tggaaaaag 
      actcaataa ccgaagttt acgtttcaa atattgaac 
      caaacggtg acgtgtatg tccaggtcc gtactgtcg 
      gttatttca gtttgatgg aagatttga agaatttaa 
      ccgataata taacgtcta aaaacccct cccagcttt 
      ctactacca caccaacca aagacaaca gcaccggta 
      aaatatacc tagtgatgt gtaaaatga cgcaacaat 
      ggctcagag caacgattg ttgattggt caccgaaag 
      gtgacttag cacaagtgt atttcattg atcaaggaa 
      atgcccaag ttgtgaatg tgaccaatg aaaaaatgt 
      acgaactgg ataagttag acagtgtct taagtcagt 
      atccaggtc aggcgatta aagttcgct tcatgattg 
      agaaaatcc caagtgatt ttgtgcaaa aagccgaga 
      aattgctac cgaaagatc ggccagttt tgctcaaaa 
      ttatcagtt atgataatg agaatgtgg cctgggttg 
      agtttttca aacggacga cagatggcg tacttgttt 
      tcatcaata aatctatct cggtcgaag ccgagttgc 
      aacaaaacg ttgacgtta gcaataata acgagacca 
      accgcaaaa gactgctca atttggtgc aattcaacg 
      aagccagca aaaatgtcc catctgggg gctctctcc 
      gaaagcccg atttgccca agatgggcg actatttca 
      acgtcaata taccatttg ctgtgaatc cctggaatt 
      tctttgtgc aaccgctgg attcgttcg cacgtctca 
      aggcgttaa tgaacgaaa tgcaggagc attacaagg 
      acacccatt ttagtccaa tgccacttg aagagataa 
      ttcctggta aaatttacg cgtcgaaac acgaagatg 
      ggcaatggt acagaacca acgtcttga aggtcatcc 
      acgagggtt cgatcagtg ttttttact gcgattttg 
      gttattaca ccaacttga cactggatc agttggtac 
      cactggatg ccaagtaca tgggtttgc cctaccagg 
      cgctcaagg cgaaaattt ccggaatta agccgatta 
      aaaacaagt ggacgatgg aagactgcg agagtttta 
      aagatctga ttttgaaga aacagtttg ttggtgtta 
      ttacgaata tagacaggg acgagtttc ataaaagtg 
      atcttatcc tggaagtgt tactgattg acacttcca 
      gtgaggaag atgttaata ttaaggaag tactcatcc 
      agaaaggta ttgcaacta gtgtgtaag cttcatttc 
      gaattccta aaaatagta tatttttgt atggttcaa 
      gttttgtgt ttgaattta ttaagaggt atcagtatt 
      gtttctgat agtggttgt tgccataaa ttccaacgt 
      tttaggtta ttgttttgg gccttcacc aaagaatgt 
      ttgttgttt ttttactag atttattgc tgttaatta 
      ccatgttac agctatgaa cacgtttaa taaataccc 
      tttcatttc
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