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Gene information
General information
TC015211 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1464348..1465586
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015211
    iBeetle screen iB_02429

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    egg chamber not present - Number of animals showing the phenotype: 1

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber early shape irregular - Number of animals showing the phenotype: 3
    vitellogenic egg chamber degenerated - Number of animals showing the phenotype: 3
    yolk fragmented - Number of animals showing the phenotype: 3
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015211 RA
      MAVLPPDPV FCLKSDMGH IHSLCFPTT TEDYASRLL 
      AATESGFVY FWDLETNRL QHKQSMGES IQAVHSISH 
      DIITQEKVG MVKFWTITN SSYQLSTSY TCRGGYCRS 
      ILLNDNLIV PQEDSTLDI ISIKTMSKT ARLVPLKHQ 
      LGNVMCLQK VELGGKTYI LGGFETGDI VLWDYTTAQ 
      PCGHLKLRE CITSLTFDP VTGRGICGN ASNTLQIFT 
      IDKSFNITL KCEISITNE GCNIVKLRD DRKIFVAGN 
      WDGSLRLFS WKSLRLLVV LNEHKGAVT DVQFSVKPV 
      RFWDANIMA ASGADGVIS LWNLYN
    CDS sequence
    • >TC015211 RA
      atggcggtt ttgcccccc gaccctgtc ttttgtcta 
      aaaagcgac atggggcac attcacagt ctttgcttc 
      cccacaact accgaagac tacgcctca agactgctg 
      gcagcgact gagagcggc ttcgtgtat ttttgggac 
      ttggagact aatcgcctc caacacaaa caatcaatg 
      ggcgagtca atccaggcc gtccactcc atatcacac 
      gacataatc acacaggaa aaagtcggc atggtcaaa 
      ttctggacc attaccaac tcctcctac caactctcc 
      acaagctac acttgtcgc gggggctac tgccgcagt 
      atcctcctc aacgacaac ctcatcgtg ccccaagag 
      gacagcacg ctcgacata atcagcatt aaaaccatg 
      tcgaaaacc gcccgtctg gtgcccctc aagcaccag 
      ctgggcaac gtcatgtgc ctccagaag gtggagctc 
      ggggggaaa acctacatt ctggggggg tttgagacg 
      ggggacatc gtcctgtgg gactacacc acggcgcaa 
      ccttgcggc cacttaaaa ctgcgcgaa tgtatcacg 
      tcgcttact tttgacccg gtgacgggg cgcggtatt 
      tgcgggaac gcctcgaac acgctccag atttttaca 
      attgataag agttttaat attacgctt aaatgcgaa 
      atttccatc acgaatgaa gggtgcaat attgttaag 
      ttgcgggat gatcggaag atttttgtg gcggggaat 
      tgggacggg agtttgagg ctcttctcc tggaagagt 
      ctcaggctt ttggtcgtt ttgaacgag cataagggg 
      gctgtgacc gatgtgcag ttttcggtc aagccggtc 
      aggttctgg gatgctaat attatggca gccagtggc 
      gccgatggc gttatttcc ttgtggaat ttgtacaat 
      taa
    mRNA sequence
    • >TC015211 RA
      tacgcgtct agtggcagt gaggttatg taaaaaaaa 
      ttgtttttg cgtaaaaca ataattaaa cgttggact 
      aatcgttta aagatagtg aatatgtaa attaagtga 
      ttttcgtcg attgatata cacaacgat ggcggtttt 
      gccccccga ccctgtctt ttgtctaaa aagcgacat 
      ggggcacat tcacagtct ttgcttccc cacaactac 
      cgaagacta cgcctcaag actgctggc agcgactga 
      gagcggctt cgtgtattt ttgggactt ggagactaa 
      tcgcctcca acacaaaca atcaatggg cgagtcaat 
      ccaggccgt ccactccat atcacacga cataatcac 
      acaggaaaa agtcggcat ggtcaaatt ctggaccat 
      taccaactc ctcctacca actctccac aagctacac 
      ttgtcgcgg gggctactg ccgcagtat cctcctcaa 
      cgacaacct catcgtgcc ccaagagga cagcacgct 
      cgacataat cagcattaa aaccatgtc gaaaaccgc 
      ccgtctggt gcccctcaa gcaccagct gggcaacgt 
      catgtgcct ccagaaggt ggagctcgg ggggaaaac 
      ctacattct gggggggtt tgagacggg ggacatcgt 
      cctgtggga ctacaccac ggcgcaacc ttgcggcca 
      cttaaaact gcgcgaatg tatcacgtc gcttacttt 
      tgacccggt gacggggcg cggtatttg cgggaacgc 
      ctcgaacac gctccagat ttttacaat tgataagag 
      ttttaatat tacgcttaa atgcgaaat ttccatcac 
      gaatgaagg gtgcaatat tgttaagtt gcgggatga 
      tcggaagat ttttgtggc ggggaattg ggacgggag 
      tttgaggct cttctcctg gaagagtct caggctttt 
      ggtcgtttt gaacgagca taagggggc tgtgaccga 
      tgtgcagtt ttcggtcaa gccggtcag gttctggga 
      tgctaatat tatggcagc cagtggcgc cgatggcgt 
      tatttcctt gtggaattt gtacaatta attgcgact 
      gaattgacg attttcaaa taattttat gtgagaaat 
      aagttgttg aaactttct gagtgtatt tttgttaaa 
      taaataaaa cttaatgag ctta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY