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Gene information
General information
TC015203 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1565076..1569137
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015203
    iBeetle screen iB_02428

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Pupal morphology
    urogomphi proximal broader - Number of animals showing the phenotype: 2

    Adult morphology
    abdominal odoriferous glands shape irregular - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    abdominal odoriferous glands content irregular - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection



    abdominal odoriferous glands partially not present - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015203 RA
      MGDSQICKF YDGQNVLVT GGTGFMGKI LIEKLLRST 
      DVATIFLLI REKKGKNVH TRLDDIFDN IIFERLKKE 
      RPKFRHRVV AVAGDCSIS GLGLTITDR QKLMSEVHI 
      AFHVAATVR FDENLKLSY SINVKGTAD VIELCRQMK 
      NLKSLIHVS TAYSNCHLD SIDETFYDY PVDYEKVGA 
      LLEKVSKSE ADKLTPRVI GKWPNTYTF TKALAEALI 
      RNTATSLPV GIFRPAIVI STYKEPMES WIDNLYGPT 
      GAVAGAASG LLRVFPCNE DVVADIVPV DTCVAGIIA 
      AAWDVTNKR NERTSPSIP IYNYVSSVS NSVTWNEYI 
      TLNKIHGTK YPLLKALWT IKIAAISDP RLYLLMRIF 
      LHLIPAFFL DFCAIIVGQ KPRLVSMYS KIHKFSDVI 
      AFFCTREWK FTNDNVENL WEKMNTADK ELFPLSITT 
      VPWITYFRG YFKGIRVHL LNDPMSTLD EARARKRKF 
      DFAHNGLKF LFVFIIVTL FWIVVFKTA ALFSSDTEA 
      VTFSNSTDN FLTDVLNS
    CDS sequence
    • >TC015203 RA
      atgggggat tcccagatt tgtaaattc tacgatggg 
      cagaatgtg ctagtgacc ggcgggaca ggctttatg 
      gggaaaatt ctcatcgaa aagttgctg agaagtaca 
      gacgttgcg acgattttt ctactgata agggagaag 
      aaagggaag aatgtgcat acgagactt gacgatatt 
      tttgacaat attattttt gaacggttg aaaaaagag 
      cgcccaaaa ttccgccac cgtgtggtg gctgttgcc 
      ggcgattgt tccatttcc ggtctaggt ttgaccatc 
      acagaccgt cagaaatta atgtcagaa gtacatatt 
      gcatttcat gtcgcggca actgtgcga ttcgacgaa 
      aatttaaaa ctttcgtat tcaatcaac gttaagggg 
      acagctgat gtgatcgaa ctttgccgc caaatgaaa 
      aatttgaag tccctaata cacgtgtcc acggcctac 
      tccaactgc cacttggac tcgattgac gaaactttc 
      tacgactac ccggtggat tacgaaaaa gttggagct 
      ctgctggag aaggtttcc aaaagcgaa gccgacaaa 
      ctcacaccc agagttata ggcaaatgg cccaacacc 
      tacacgttc accaaagct ttggccgaa gctttgatc 
      agaaacacc gccacgagt ttgcctgtc gggattttt 
      cgaccagcg atcgttatt tcaacgtac aaagaacca 
      atggagagt tggattgat aatttgtat ggacctacg 
      ggggctgta gctggggct gcttctgga cttctccgt 
      gtttttcca tgtaatgag gatgtggtg gctgatatt 
      gtaccagtt gacacgtgt gtggccgga attatcgca 
      gcggcgtgg gatgtgaca aataaaagg aacgaaagg 
      acgtccccc agcatcccc atctacaac tacgtctcc 
      tccgtcagc aactccgtg acttggaac gaatacata 
      accctcaat aaaatccac ggcacaaaa taccctctc 
      ctgaaggcc ctctggacc attaaaatc gccgccatc 
      agcgacccc cgcctttac ctcctaatg cgaattttc 
      ctccactta atcccggcc tttttcctg gatttttgt 
      gccataatc gtcggccaa aagccacgt ctagtctca 
      atgtacagc aaaatccac aaattttcc gacgttatc 
      gcctttttc tgtacaaga gaatggaaa ttcactaat 
      gataacgtg gaaaatctt tgggagaaa atgaacacc 
      gctgataag gaactcttc cctttgagc atcacgaca 
      gtgccttgg atcacctac tttcgaggc tatttcaaa 
      gggataagg gtgcacttg ttgaacgat cccatgagc 
      acgctggac gaggctagg gccaggaaa agaaagttc 
      gattttgca cacaatggt ctcaagttt ttgttcgtt 
      tttattatt gttacgctg ttttggatt gttgttttt 
      aaaactgcg gctttgttt agcagtgac actgaagcg 
      gtaacgttt agtaatagt acagataat tttttaaca 
      gatgtttta aacagctag
    mRNA sequence
    • >TC015203 RA
      tatctgcta gcaattttt tgctaaaga aggcttaac 
      tttatggtt tgatacaat ttttatttg tttttggta 
      tacaaggaa attaaatat atacatata atttattat 
      tgatttatt attgaacgc catttcacg gttggactt 
      ttgttaatc gtgtctgtg tgcataagg gggccacca 
      cccccccat acctcccac tatcactta aaagcatcg 
      caggatgca cttgtttca ttttatgac agtttagac 
      cagtcacta gaccaattg taaaatgtc tcgtcgatc 
      ctagtgtgt ctaacgtgt tgtgtataa ttttatttc 
      gcaaaaaaa attaaatta aaccaagtt ttacaaaat 
      gggggattc ccagatttg taaattcta cgatgggca 
      gaatgtgct agtgaccgg cgggacagg ctttatggg 
      gaaaattct catcgaaaa gttgctgag aagtacaga 
      cgttgcgac gatttttct actgataag ggagaagaa 
      agggaagaa tgtgcatac gagacttga cgatatttt 
      tgacaatat tatttttga acggttgaa aaaagagcg 
      cccaaaatt ccgccaccg tgtggtggc tgttgccgg 
      cgattgttc catttccgg tctaggttt gaccatcac 
      agaccgtca gaaattaat gtcagaagt acatattgc 
      atttcatgt cgcggcaac tgtgcgatt cgacgaaaa 
      tttaaaact ttcgtattc aatcaacgt taaggggac 
      agctgatgt gatcgaact ttgccgcca aatgaaaaa 
      tttgaagtc cctaataca cgtgtccac ggcctactc 
      caactgcca cttggactc gattgacga aactttcta 
      cgactaccc ggtggatta cgaaaaagt tggagctct 
      gctggagaa ggtttccaa aagcgaagc cgacaaact 
      cacacccag agttatagg caaatggcc caacaccta 
      cacgttcac caaagcttt ggccgaagc tttgatcag 
      aaacaccgc cacgagttt gcctgtcgg gatttttcg 
      accagcgat cgttatttc aacgtacaa agaaccaat 
      ggagagttg gattgataa tttgtatgg acctacggg 
      ggctgtagc tggggctgc ttctggact tctccgtgt 
      ttttccatg taatgagga tgtggtggc tgatattgt 
      accagttga cacgtgtgt ggccggaat tatcgcagc 
      ggcgtggga tgtgacaaa taaaaggaa cgaaaggac 
      gtcccccag catccccat ctacaacta cgtctcctc 
      cgtcagcaa ctccgtgac ttggaacga atacataac 
      cctcaataa aatccacgg cacaaaata ccctctcct 
      gaaggccct ctggaccat taaaatcgc cgccatcag 
      cgacccccg cctttacct cctaatgcg aattttcct 
      ccacttaat cccggcctt tttcctgga tttttgtgc 
      cataatcgt cggccaaaa gccacgtct agtctcaat 
      gtacagcaa aatccacaa attttccga cgttatcgc 
      ctttttctg tacaagaga atggaaatt cactaatga 
      taacgtgga aaatctttg ggagaaaat gaacaccgc 
      tgataagga actcttccc tttgagcat cacgacagt 
      gccttggat cacctactt tcgaggcta tttcaaagg 
      gataagggt gcacttgtt gaacgatcc catgagcac 
      gctggacga ggctagggc caggaaaag aaagttcga 
      ttttgcaca caatggtct caagttttt gttcgtttt 
      tattattgt tacgctgtt ttggattgt tgtttttaa 
      aactgcggc tttgtttag cagtgacac tgaagcggt 
      aacgtttag taatagtac agataattt tttaacaga 
      tgttttaaa cagctagct taggtttgt aagttatgg 
      ttattgtgc caataaaat tttcagctt ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY