Status
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Gene information
General information
TC015199 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1618960..1619977
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015199
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02427

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015199 RA
      MRYAQLVIG PAGSGKSTY CSALVQHAV DAKRTIEVV 
      NLDPAAEHF DYEPRADIR ELIHVQDTM EDEELHFGP 
      NGGLVFCLE YLLENSDWL RDKLGEEED DYILFDCPG 
      QIELYTHLT AMKKLVKLL QDWNFNICS IFLVDVQFM 
      TDGAKFLSG TMAALSVMV NLELPHVNL LSKMDLLSK 
      GARKRLDRF LEPDSHAIL GDIELSGMN AFNEKYKKL 
      SEAIGDLIE NYSLVRFFP LNLKNHESV SDILVTIDN 
      VIQYGEDQD VRTRDFEEQ DDE
    CDS sequence
    • >TC015199 RA
      atgcggtac gcgcagcta gttattggc cccgcgggc 
      agtggcaag tcgacgtat tgttcggct ttggtgcaa 
      cacgcagtc gacgcaaaa cgcaccata gaagtggta 
      aatttggac ccagcggcc gaacacttc gactacgag 
      ccacgtgcc gacatccgc gagttaatc cacgtgcag 
      gacaccatg gaggacgaa gagttgcac tttggcccc 
      aacgggggg cttgtcttt tgcctggag tacctgctg 
      gagaactcc gactggttg cgagacaag ttgggggag 
      gaggaggac gattatatt ttgtttgac tgtccgggt 
      caaatcgaa ctatacacg cacttaaca gctatgaaa 
      aaattagtc aagttgtta caagactgg aattttaat 
      atttgtagt atttttttg gtggacgtt cagtttatg 
      acagacggg gccaagttt ttgtcgggc accatggct 
      gcactcagt gtcatggtt aatttagag ctgcctcat 
      gtgaatttg ttgtcgaaa atggatttg ttgagcaag 
      ggggcgcgc aagcgcctc gataggttc ctcgagcct 
      gattcgcat gccattctg ggggatatt gagttaagt 
      ggcatgaat gccttcaat gagaaatac aaaaaattg 
      tctgaagcc ataggggat ttaattgag aattattcc 
      ctagtcagg tttttcccc ctcaatttg aaaaaccac 
      gaaagtgtg agtgacatt ttggtgacc attgacaac 
      gtgatacag tatggcgag gatcaggac gtcaggaca 
      cgtgatttc gaggaacag gacgacgaa taa
    mRNA sequence
    • >TC015199 RA
      tcacaacac aacctcact tattgcatg gtggtaaca 
      ctggtcgtt acctaacct aattttttg cgtttgttt 
      tttgtaaaa atgcggtac gcgcagcta gttattggc 
      cccgcgggc agtggcaag tcgacgtat tgttcggct 
      ttggtgcaa cacgcagtc gacgcaaaa cgcaccata 
      gaagtggta aatttggac ccagcggcc gaacacttc 
      gactacgag ccacgtgcc gacatccgc gagttaatc 
      cacgtgcag gacaccatg gaggacgaa gagttgcac 
      tttggcccc aacgggggg cttgtcttt tgcctggag 
      tacctgctg gagaactcc gactggttg cgagacaag 
      ttgggggag gaggaggac gattatatt ttgtttgac 
      tgtccgggt caaatcgaa ctatacacg cacttaaca 
      gctatgaaa aaattagtc aagttgtta caagactgg 
      aattttaat atttgtagt atttttttg gtggacgtt 
      cagtttatg acagacggg gccaagttt ttgtcgggc 
      accatggct gcactcagt gtcatggtt aatttagag 
      ctgcctcat gtgaatttg ttgtcgaaa atggatttg 
      ttgagcaag ggggcgcgc aagcgcctc gataggttc 
      ctcgagcct gattcgcat gccattctg ggggatatt 
      gagttaagt ggcatgaat gccttcaat gagaaatac 
      aaaaaattg tctgaagcc ataggggat ttaattgag 
      aattattcc ctagtcagg tttttcccc ctcaatttg 
      aaaaaccac gaaagtgtg agtgacatt ttggtgacc 
      attgacaac gtgatacag tatggcgag gatcaggac 
      gtcaggaca cgtgatttc gaggaacag gacgacgaa 
      taaaattcc ctagagtgt ttattccaa tttatactt 
      ttttttata taaaacaat aacacaaaa tactaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY