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Gene information
General information
TC015191 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1710328..1712485
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015191
    iBeetle screen iB_02425

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015191 RA
      MNALGLSMI VKMGCICTR ESVDVNGVK YVVKERLGE 
      GGFSTVDLI ENKGTKRKY ALKRILCHS LEDQKVAMD 
      EISYYKKLR HPNIIELVD STFKGTADI VVNATSEAY 
      IVLPYYRRG TLQDYLTLR SFNRNYLDV KEVLRLFSE 
      ISQAVKYLH DFAPEPVAH RDLKAGNVC LTEDMTPVL 
      MDLGSCAPA KVQVCGAQD AQKLQDLAA ERCSMTYRA 
      PELFHVESY CVIDQRTDI WSLGCLLFA LCYFKSPYD 
      LVYERGDSV NLAVISGSV HFPEDTPFD EDVHDLILF 
      MLKVNPSER PFIDSVIDK TRHLQNKIE SVV
    CDS sequence
    • >TC015191 RA
      atgaacgct ttggggctc agtatgatc gtcaaaatg 
      gggtgcatt tgcacaagg gagtcggtc gacgtaaat 
      ggggtgaag tacgtcgtg aaggaacgc ctgggtgaa 
      gggggcttc tctaccgtc gatctgatc gaaaacaag 
      ggcaccaag cgcaagtac gcccttaaa cggatcctc 
      tgccacagt ctcgaggac cagaaagtg gcaatggac 
      gaaatttcc tactacaaa aaattgaga caccccaat 
      attatcgaa cttgtggac tccacattt aaaggcacg 
      gccgatatt gtggtaaat gccacctcg gaggcctac 
      atagtcctg ccgtattat cgccgaggc actttacaa 
      gattaccta actttgcgt tccttcaac cgaaattat 
      ttagacgtg aaggaagtg ttgcgatta ttcagtgaa 
      atcagccag gctgttaag tatttgcac gactttgcc 
      cccgagccg gtggcgcat cgcgacctc aaggccggg 
      aacgtgtgt ctgaccgag gacatgacc cccgttttg 
      atggacttg ggctcctgt gccccagcc aaagtccag 
      gtttgtggg gctcaggac gcccagaaa ttacaagac 
      ttggcggca gaacgctgc tcgatgact taccgcgcc 
      cccgagttg ttccacgtc gagagttac tgtgtcata 
      gaccagaga acggacatt tggagtctc gggtgtctt 
      ttattcgct ttgtgttac tttaaatcg ccgtacgat 
      ttggtgtac gaacgcggc gattcggtc aatttggct 
      gttatttcc gggagtgta cattttccg gaagacacg 
      ccctttgat gaggacgtg cacgacttg attctcttc 
      atgcttaaa gtcaatccg tctgaaagg cctttcatc 
      gacagtgtc atcgacaag acgcgccat ttgcaaaac 
      aaaatcgaa tcagttgtg taa
    mRNA sequence
    • >TC015191 RA
      gctgccaag ggcggcgcg gcaaatgct ctaattcag 
      tatattttc gatctcata tgtaaactc tacatgctg 
      taagttatt ttttaaaat acaaataat taaaaaata 
      aacggtcca agttgataa aactcgtat ttgagtctt 
      tacttccgg ttttggcca cgaaaaaaa tttcaaata 
      tttttttaa ttttcccct tcacatgaa acgccccat 
      gcttacctg tccggtgaa aataataac agcaacgag 
      tccaaacca cgaatgcaa cgtgtacag gttcggttt 
      cgggggctt agcataatt atgcgagtc aaaagctgc 
      cttaagtcc aatcaccgc caaaataaa cgccgacaa 
      atgcagcaa agcgtgcgt gactttcag gcgtttttt 
      cgtggcatt acgtcccgt ccggtagat taaaatcgc 
      tgtagaaaa aaacaaatc aagggggcc ccctcaaaa 
      accgacata cattgaccg tagagaaaa actaggcct 
      gtgaccatc aaaaggggg tgccagttg aactgcagc 
      tgggggtcg gaccacgag aacccgtcg cggtacttg 
      accaaccat gacagtacc atcacgagc atgcccacc 
      cccagggct gtcgcaaaa ctgtacaac gagcggtaa 
      ttgcgcact ttgaggtgc tcgttgcgg gcctccatt 
      ccactaaac acaaacacc actcaaatc aaaacttcg 
      gacgataca aacctgaga agaccgaca gattgttcc 
      gaactcatt caacaagtg gacgaaaaa tcaataacc 
      ccctcccaa gtcccaagt cctgttacg tgacacaaa 
      agcgataaa actcaaacc gtaacacaa cacaatgca 
      ctaattcgc gcgtgaaag gccgggaaa cggtaaaac 
      gcagcaatg tttgacggc caaaagtga ggttatgtc 
      aattgtcag acgtgggag ttttcgccg aaatgaacg 
      ctttggggc tcagtatga tcgtcaaaa tggggtgca 
      tttgcacaa gggagtcgg tcgacgtaa atggggtga 
      agtacgtcg tgaaggaac gcctgggtg aagggggct 
      tctctaccg tcgatctga tcgaaaaca agggcacca 
      agcgcaagt acgccctta aacggatcc tctgccaca 
      gtctcgagg accagaaag tggcaatgg acgaaattt 
      cctactaca aaaaattga gacacccca atattatcg 
      aacttgtgg actccacat ttaaaggca cggccgata 
      ttgtggtaa atgccacct cggaggcct acatagtcc 
      tgccgtatt atcgccgag gcactttac aagattacc 
      taactttgc gttccttca accgaaatt atttagacg 
      tgaaggaag tgttgcgat tattcagtg aaatcagcc 
      aggctgtta agtatttgc acgactttg cccccgagc 
      cggtggcgc atcgcgacc tcaaggccg ggaacgtgt 
      gtctgaccg aggacatga cccccgttt tgatggact 
      tgggctcct gtgccccag ccaaagtcc aggtttgtg 
      gggctcagg acgcccaga aattacaag acttggcgg 
      cagaacgct gctcgatga cttaccgcg cccccgagt 
      tgttccacg tcgagagtt actgtgtca tagaccaga 
      gaacggaca tttggagtc tcgggtgtc ttttattcg 
      ctttgtgtt actttaaat cgccgtacg atttggtgt 
      acgaacgcg gcgattcgg tcaatttgg ctgttattt 
      ccgggagtg tacattttc cggaagaca cgccctttg 
      atgaggacg tgcacgact tgattctct tcatgctta 
      aagtcaatc cgtctgaaa ggcctttca tcgacagtg 
      tcatcgaca agacgcgcc atttgcaaa acaaaatcg 
      aatcagttg tgtaattat ttatttttc tacatgcgt 
      caatgtaaa tacactat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY