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Gene information
General information
TC015188 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1744661..1748850
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015188
    iBeetle screen iB_02423

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015188 RA
      MNRGEESLR LMRESGEIK WCPREKSQI AEFYKEKNV 
      LVTGATGFL GKLLVEKLL RACPDLGTI YILVRPKKG 
      EDEHSRVDK LYSDPIFGP LTKLFPKFQ HKISIIKGD 
      VSLPDLGLS PSSKSLIYE KINVIFHIA ATVRFDEKI 
      TVATAINVR GTRDLLNMA KKCQNLQSF VHVSTAYAN 
      CVQDEIDEV FYKMPISAH DLIELVETK PEATLIEQT 
      SRIIQNWPN TYTFTKGVA EDAVKNFGK GLPVCVVRP 
      AIVIATYKE PLRSWIDNL YGATGIVVG AGTGLLKTM 
      HCDRSKTAE LVPGDYVIN NMIAASYKT AVEKITDKI 
      PVYNYVSSV ENHHTWNDF MQKCQLWGE EVPTIRCVW 
      YYCLILNKF YWAHFLLSI FLHFLPALI MDLGMVLSR 
      QRPIMVKIY KKITKFESV ISHFSTNEW KFHNDNTQA 
      LWNSLGEDD RAMFPFSVK ELDWDEYHK THALGLRQY 
      LVKDDISTL PQARIKWRR FYVANKVIK YLVLSVIFY 
      VIYSIFVRF CNY
    CDS sequence
    • >TC015188 RA
      atgaatcgt ggcgaggaa tcgttgcgt ctgatgcgc 
      gaatcgggt gaaattaaa tggtgtcca cgtgaaaaa 
      tcacaaatc gccgaattt tacaaagaa aaaaatgtc 
      ctggtgacg ggcgccact ggatttttg ggcaaactt 
      ctagtggag aaactgcta agggcctgc cctgacttg 
      ggcacaatc tacattctg gtgagaccg aaaaaaggc 
      gaagacgaa cacagcaga gtggataag ctgtatagc 
      gacccgatt tttggacct ttgactaaa ctttttccc 
      aagtttcaa cacaaaatc agcattatc aagggcgat 
      gtttcgctg cctgacctt gggctaagc ccttcaagc 
      aaaagtcta atttacgaa aagatcaat gtcattttc 
      cacattgcg gcaacggtg cgatttgac gaaaaaatc 
      acagtcgcc actgcaatc aacgttcga ggcacacgt 
      gatttatta aacatggca aaaaagtgc caaaattta 
      cagtcattt gtccacgtc agtacagcc tacgccaac 
      tgcgtccaa gacgaaatc gacgaagtt ttctacaaa 
      atgccaatt tcagcgcac gacttgatc gaactcgtc 
      gaaacgaaa ccagaggcc actttgatc gaacaaacg 
      agcagaatt atacaaaac tggccaaac acttatact 
      tttacaaag ggcgtagcg gaagacgct gtcaaaaat 
      ttcggaaaa gggcttccg gtttgcgtc gtgcgccca 
      gcgattgtt attgcaact tataaggag ccgttacgt 
      agctggatt gataatttg tacggggcg actgggatc 
      gtagtaggc gccgggacc ggcctactt aaaactatg 
      cattgtgac aggagcaaa acagccgaa ttggtccca 
      ggcgactat gtgatcaat aacatgatc gcagcgtcg 
      tataaaacg gcagtagaa aaaataacg gataaaatt 
      ccggtgtac aattacgtt tcaagtgtt gaaaatcac 
      cacacttgg aacgatttt atgcaaaag tgtcaattg 
      tggggggag gaggtgcca acaatcaga tgtgtgtgg 
      tactactgt ttgatattg aacaagttc tactgggct 
      cattttttg cttagtatt tttttgcac tttttgccc 
      gccctgatc atggatttg gggatggtg ttgtcaagg 
      caacggcca ataatggtg aaaatttac aaaaaaatt 
      acgaaattc gaaagtgtc atttcgcat ttctccacc 
      aacgagtgg aagttccac aatgataac acgcaagcc 
      ctttggaat agtctgggt gaagatgat agggctatg 
      tttcccttt agtgtgaag gagttggat tgggatgag 
      taccataag acccatgcc ttgggtttg cggcaatat 
      ttggtcaaa gatgatatt tcaacgctg ccacaagcc 
      agaatcaag tggaggagg ttttatgtg gccaataaa 
      gtcatcaaa tatcttgtt ctgagtgtg atattttac 
      gtaatttat tcgattttt gtaagattt tgtaattat 
      tga
    mRNA sequence
    • >TC015188 RA
      tgtccgaaa acgacttcc actgtaaca aaagcctgc 
      agaaacaaa cgcgtgtgc tgtgtccaa gtaactcca 
      attaaatta atccaaatt aattaaaat gaatcgtgg 
      cgaggaatc gttgcgtct gatgcgcga atcgggtga 
      aattaaatg gtgtccacg tgaaaaatc acaaatcgc 
      cgaatttta caaagaaaa aaatgtcct ggtgacggg 
      cgccactgg atttttggg caaacttct agtggagaa 
      actgctaag ggcctgccc tgacttggg cacaatcta 
      cattctggt gagaccgaa aaaaggcga agacgaaca 
      cagcagagt ggataagct gtatagcga cccgatttt 
      tggaccttt gactaaact ttttcccaa gtttcaaca 
      caaaatcag cattatcaa gggcgatgt ttcgctgcc 
      tgaccttgg gctaagccc ttcaagcaa aagtctaat 
      ttacgaaaa gatcaatgt cattttcca cattgcggc 
      aacggtgcg atttgacga aaaaatcac agtcgccac 
      tgcaatcaa cgttcgagg cacacgtga tttattaaa 
      catggcaaa aaagtgcca aaatttaca gtcatttgt 
      ccacgtcag tacagccta cgccaactg cgtccaaga 
      cgaaatcga cgaagtttt ctacaaaat gccaatttc 
      agcgcacga cttgatcga actcgtcga aacgaaacc 
      agaggccac tttgatcga acaaacgag cagaattat 
      acaaaactg gccaaacac ttatacttt tacaaaggg 
      cgtagcgga agacgctgt caaaaattt cggaaaagg 
      gcttccggt ttgcgtcgt gcgcccagc gattgttat 
      tgcaactta taaggagcc gttacgtag ctggattga 
      taatttgta cggggcgac tgggatcgt agtaggcgc 
      cgggaccgg cctacttaa aactatgca ttgtgacag 
      gagcaaaac agccgaatt ggtcccagg cgactatgt 
      gatcaataa catgatcgc agcgtcgta taaaacggc 
      agtagaaaa aataacgga taaaattcc ggtgtacaa 
      ttacgtttc aagtgttga aaatcacca cacttggaa 
      cgattttat gcaaaagtg tcaattgtg gggggagga 
      ggtgccaac aatcagatg tgtgtggta ctactgttt 
      gatattgaa caagttcta ctgggctca ttttttgct 
      tagtatttt tttgcactt tttgcccgc cctgatcat 
      ggatttggg gatggtgtt gtcaaggca acggccaat 
      aatggtgaa aatttacaa aaaaattac gaaattcga 
      aagtgtcat ttcgcattt ctccaccaa cgagtggaa 
      gttccacaa tgataacac gcaagccct ttggaatag 
      tctgggtga agatgatag ggctatgtt tccctttag 
      tgtgaagga gttggattg ggatgagta ccataagac 
      ccatgcctt gggtttgcg gcaatattt ggtcaaaga 
      tgatatttc aacgctgcc acaagccag aatcaagtg 
      gaggaggtt ttatgtggc caataaagt catcaaata 
      tcttgttct gagtgtgat attttacgt aatttattc 
      gatttttgt aagattttg taattattg agtgggttt 
      ttaataaaa tagtttttg taat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY