Status
Please wait ...
Gene information
General information
TC015184 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1982003..1985784
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015184
    iBeetle screen iB_02422

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015184 RA
      MAETSPSVH QRDLSNIKR LLWSTDIKA DIFRRWSQG 
      FYFSASEPT ALEQAEGGP CAIIAPVQA FILKNLLLK 
      YKDLSFREM AVTSDMQTH LLVNALCEI LEQCTGRKY 
      FLVYLSDTI SDQVVQNGV VSQQHTESE STVFHEGLR 
      IHVFQGVEE VRKYYIENI NHLKSQYGI LLFLYSVIL 
      TRGLEEVQS DNSNDTSEP LIDDTYGYG SQSLINLMI 
      TGRATTYVW DHEQDVGGL KLKGLEKQS QIGFITIME 
      HLRYCTVGS FYKNPIHPV WVLGSDTHL TVLFSTERR 
      LVSPETKTD QAKRVFKHF DPDGNNFIS SSLLQDVLQ 
      ALDLVSEEE YVEVMRKKL DPENLGIIL LNSFMDEFF 
      PKEDNPMPD VFSLVHYNS LAQSNLDGQ IRYRIGECV 
      LLESDLRAV SESNPMLTV LQTKWPNIE VRWNELGTP 
      SLN
    CDS sequence
    • >TC015184 RA
      atggccgag acatcaccg tcggtccac cagcgcgac 
      ctctccaac atcaaacgc ctgttgtgg agcaccgac 
      atcaaggcg gacattttc cgccgttgg tctcaaggc 
      ttctatttc agtgcaagc gaaccgacg gccttggaa 
      caggccgaa gggggccct tgtgccatc atcgccccg 
      gttcaagcc ttcattttg aaaaatttg ttactcaaa 
      tataaggat ttatcattc cgggagatg gccgtcact 
      agtgacatg cagacgcat cttctcgtc aacgccttg 
      tgcgagata ttggaacag tgcaccggt cgcaaatat 
      tttttagtg tatttgagt gacacaata agtgaccaa 
      gtggtgcaa aatggggtt gtgtcacaa cagcacacc 
      gaatccgaa tcgacggtt tttcacgag gggctccga 
      atacacgtg tttcagggc gtggaggag gttaggaag 
      tattacatt gagaatatc aaccacttg aagtcacaa 
      tacggaatt ttgttattt ttatattcg gtcattttg 
      acacgggga ttggaagag gtccaatcg gataattct 
      aatgacacc tcagagcct ttaattgat gacacttat 
      ggttatggc agccaaagt cttatcaat cttatgata 
      acgggacgt gcgacgact tatgtgtgg gaccacgag 
      caggatgtt gggggttta aaactgaaa ggtttggag 
      aagcaaagt cagatcggt tttatcaca ataatggag 
      cacttgagg tattgtacg gtgggttca ttttacaaa 
      aatccgatt catccggtt tgggttttg ggctctgac 
      acacactta acagtatta tttagtaca gaaagacgg 
      ctcgtgagc cccgaaact aaaaccgac caagcgaag 
      cgagtgttt aaacatttc gatcctgac ggaaataac 
      ttcataagt tcgtctcta ttgcaagat gtgctccaa 
      gcccttgat ctagtcagt gaagaggag tatgtagaa 
      gtcatgcgg aaaaaactg gacccggaa aatttaggc 
      ataattctg ttaaatagt tttatggat gagtttttc 
      cccaaggag gataatcca atgcccgat gtgttttca 
      ttggtgcat tacaatagc ctggcgcag agtaatttg 
      gacggacaa atacggtac aggataggt gaatgcgtt 
      ttattggag tcggacttg agggccgtc agtgagtca 
      aatcccatg ttgacggtg ttgcaaacc aagtggcca 
      aatatagaa gttcgatgg aacgagtta gggacacct 
      tctttgaac tag
    mRNA sequence
    • >TC015184 RA
      aacctcaca cactttccg cgttctcta ccacaaaaa 
      tcgcctttc ttttgacgt agaatggcc gagacatca 
      ccgtcggtc caccagcgc gacctctcc aacatcaaa 
      cgcctgttg tggagcacc gacatcaag gcggacatt 
      ttccgccgt tggtctcaa ggcttctat ttcagtgca 
      agcgaaccg acggccttg gaacaggcc gaagggggc 
      ccttgtgcc atcatcgcc ccggttcaa gccttcatt 
      ttgaaaaat ttgttactc aaatataag gatttatca 
      ttccgggag atggccgtc actagtgac atgcagacg 
      catcttctc gtcaacgcc ttgtgcgag atattggaa 
      cagtgcacc ggtcgcaaa tatttttta gtgtatttg 
      agtgacaca ataagtgac caagtggtg caaaatggg 
      gttgtgtca caacagcac accgaatcc gaatcgacg 
      gtttttcac gaggggctc cgaatacac gtgtttcag 
      ggcgtggag gaggttagg aagtattac attgagaat 
      atcaaccac ttgaagtca caatacgga attttgtta 
      tttttatat tcggtcatt ttgacacgg ggattggaa 
      gaggtccaa tcggataat tctaatgac acctcagag 
      cctttaatt gatgacact tatggttat ggcagccaa 
      agtcttatc aatcttatg ataacggga cgtgcgacg 
      acttatgtg tgggaccac gagcaggat gttgggggt 
      ttaaaactg aaaggtttg gagaagcaa agtcagatc 
      ggttttatc acaataatg gagcacttg aggtattgt 
      acggtgggt tcattttac aaaaatccg attcatccg 
      gtttgggtt ttgggctct gacacacac ttaacagta 
      ttatttagt acagaaaga cggctcgtg agccccgaa 
      actaaaacc gaccaagcg aagcgagtg tttaaacat 
      ttcgatcct gacggaaat aacttcata agttcgtct 
      ctattgcaa gatgtgctc caagccctt gatctagtc 
      agtgaagag gagtatgta gaagtcatg cggaaaaaa 
      ctggacccg gaaaattta ggcataatt ctgttaaat 
      agttttatg gatgagttt ttccccaag gaggataat 
      ccaatgccc gatgtgttt tcattggtg cattacaat 
      agcctggcg cagagtaat ttggacgga caaatacgg 
      tacaggata ggtgaatgc gttttattg gagtcggac 
      ttgagggcc gtcagtgag tcaaatccc atgttgacg 
      gtgttgcaa accaagtgg ccaaatata gaagttcga 
      tggaacgag ttagggaca ccttctttg aactagtga 
      caattttat ttcgcattt atttaattt ctacaatcg 
      gatataatt ttgttgttg cattttttt aataaactg 
      tacctattt ttgagcatt tagttggcg aattttgtg 
      aattcgtcg aaatgatga aacgtgcat tgttatatt 
      tgttaagcc gaaatgtat aaaaatcac aaaaatatg 
      tatattatg aaagatatt taattctgt tagtgcatg 
      tgtattttg gaaaaacaa taaaatatg ttgcatttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY