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Gene information
General information
TC015175 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 1087872..1089107
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015175
    iBeetle screen iB_02419
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015175 RA
      MCILFVYTD PTPPKGGYR LIVASNRDE YYKRPARQA 
      FKCPHTNII GGRDMEPGR EGGMWLGVS LKPQMIKFG 
      ALLNVTGAP KREAQAGRG PLVYNYLAG GEPAPEYIT 
      KLVPEQYSA FNLFMVEVS NEITCYHHS NSPEDTLTY 
      TGRQVLAFG NSTPQSPFT KVKKGGQKF EEIITNYGG 
      SRARLVQEL INLLKCEEL HLPDPELEA RAPFGVGFL 
      SSIYVRMEE GGYGTRTHS VILVDDGGN VEFVEHTMK 
      EPINPKEPQ WDVTTIKAK F
    CDS sequence
    • >TC015175 RA
      atgtgtatc ttgtttgtt tacacggac ccgacgccc 
      cccaaaggg ggctaccgc ctgatcgtg gcctcgaac 
      cgcgacgag tactacaag cggcccgcg agacaggcc 
      ttcaagtgc ccccacacg aacataatt ggaggccgc 
      gacatggag ccgggccgc gagggcggc atgtggctc 
      ggagtgtct ctcaagccg caaatgatc aagtttggg 
      gccctgctt aacgtgacc ggagccccc aaacgggag 
      gcgcaggcc gggcggggg ccccttgtc tataactac 
      ctcgcaggc ggggaaccc gcccccgaa tatatcacg 
      aaactggtg cccgaacaa tacagtgcg tttaattta 
      ttcatggtg gaagtctcg aacgaaatc acctgttac 
      caccacagt aactcccct gaagacact ctcacgtac 
      actggacgc caggtccta gccttcggg aacagtacc 
      ccacaatca ccgttcacc aaagtcaaa aaagggggg 
      caaaagttc gaggagatt atcacgaat tatgggggc 
      tcccgggca cgtcttgtc caggaactg atcaatttg 
      ttgaagtgt gaagagttg cacttgccg gatcctgaa 
      ttggaggca agggcccct ttcggggtc ggttttttg 
      agctcgatt tacgtccgg atggaggaa gggggctac 
      gggacgcgc acccactct gttattcta gtcgatgat 
      gggggcaat gtcgagttt gtggaacac acgatgaag 
      gaaccgatt aatccaaag gagccccaa tgggacgtc 
      acaacaatc aaggctaaa ttttga
    mRNA sequence
    • >TC015175 RA
      cttcaaaat cacaaagtt tactcaccc tttttgcaa 
      atctcggtc ataattcca cactcggac gtgtcacaa 
      accgaaaaa actcacaaa aaacctctc gcattgcgt 
      tagcgacga ccgtgaagt ttgaggtta tgttctaac 
      cttgagcct tgacacaaa tacaattct cgtccgaaa 
      gttgaaggt ttttctcca aatccgcgc ggatggaaa 
      caatgcaca cactaacac atgtcttat ctcgataaa 
      aatacactc agttcgtgt gtgaccgtt tcggggcca 
      aaatgtgta tcttgtttg tttacacgg acccgacgc 
      cccccaaag ggggctacc gcctgatcg tggcctcga 
      accgcgacg agtactaca agcggcccg cgagacagg 
      ccttcaagt gcccccaca cgaacataa ttggaggcc 
      gcgacatgg agccgggcc gcgagggcg gcatgtggc 
      tcggagtgt ctctcaagc cgcaaatga tcaagtttg 
      gggccctgc ttaacgtga ccggagccc ccaaacggg 
      aggcgcagg ccgggcggg ggccccttg tctataact 
      acctcgcag gcggggaac ccgcccccg aatatatca 
      cgaaactgg tgcccgaac aatacagtg cgtttaatt 
      tattcatgg tggaagtct cgaacgaaa tcacctgtt 
      accaccaca gtaactccc ctgaagaca ctctcacgt 
      acactggac gccaggtcc tagccttcg ggaacagta 
      ccccacaat caccgttca ccaaagtca aaaaagggg 
      ggcaaaagt tcgaggaga ttatcacga attatgggg 
      gctcccggg cacgtcttg tccaggaac tgatcaatt 
      tgttgaagt gtgaagagt tgcacttgc cggatcctg 
      aattggagg caagggccc ctttcgggg tcggttttt 
      tgagctcga tttacgtcc ggatggagg aagggggct 
      acgggacgc gcacccact ctgttattc tagtcgatg 
      atgggggca atgtcgagt ttgtggaac acacgatga 
      aggaaccga ttaatccaa aggagcccc aatgggacg 
      tcacaacaa tcaaggcta aattttgag gttagctgt 
      cacatggct gtcactgga gcggcagtg ataccaatt 
      ggttttaat atcgccaaa aataaaaaa ctattctcg 
      aa