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Gene information
General information
TC015170 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 1114505..1115859
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015170
    iBeetle screen iB_02418
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment partially randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015170 RA
      MSRKIGIVG SGLIGRSWA MLFANVGYQ VVIYDIEPK 
      QIESALADI EQQLKNLEK SGLLRGKLN SSQQFACIK 
      GTNSLTEAV KDAVLVQEC VPENLELKR KVWKNVDDV 
      SGPNTIFSS STSTFLPSL FSDHLKNKK NIVVSHPVN 
      PPYYVPLVE IVPAPWTDP AVTKKTRAI MEEIGQTPV 
      SLTKEVPGF VVNRLQYAL LNETWNLVS DGVLDVKDI 
      DKVMSDGLG MRYAFLGPL ETTHLNAEG FVDYCKKYS 
      NTIYSVSKD FKPLQKWEG PLVQEIAKQ LENSVPLDK 
      LQERRNWRD LALTKLSQL KKSI
    CDS sequence
    • >TC015170 RA
      atgtcgcga aaaatcgga attgtgggc agcggcctt 
      atcggccgc tcgtgggcc atgctgttc gccaatgtg 
      ggctaccag gtcgtcatc tacgacatt gagcccaaa 
      cagatcgaa tcggcgtta gccgacatc gagcaacag 
      ctcaaaaac ttggaaaaa agcgggctt ttacgcggg 
      aaattgaac tcaagccag caatttgcc tgcattaaa 
      gggaccaac agtctaacg gaggccgta aaagacgcc 
      gttttggtc caagaatgc gtccccgaa aacctggaa 
      ctgaagcga aaagtgtgg aaaaacgtg gacgacgtc 
      agcggccct aacacgatt ttctcaagc tcgacatca 
      accttcctc ccttcgcta ttctccgac catctcaaa 
      aacaagaaa aacatcgtg gtgtcgcac ccggtgaac 
      cccccgtat tacgtgccc ttggtcgag atcgtgcca 
      gcgccctgg actgacccc gcagtcacc aaaaaaacg 
      cgcgctatt atggaggaa atcgggcaa actcccgtc 
      tcactgact aaggaagtc cccggtttt gttgtaaat 
      cgcctacag tacgccctt ttgaacgaa acgtggaac 
      cttgtctcc gacggagtc ctcgatgtc aaagacatc 
      gacaaggtc atgtccgac gggctcggc atgcgctac 
      gcctttctg ggccccctt gaaaccact caccttaac 
      gccgagggc ttcgtcgac tattgcaag aaatattca 
      aacacgatt tattcagtc agcaaggac tttaaaccg 
      ttgcaaaag tgggagggc ccccttgtg caggaaatc 
      gccaaacag ttggagaat tcggtcccg ttggataag 
      ttacaggaa cgcaggaat tggcgggac ttggctctg 
      accaaactg agccaattg aaaaaatca atatag
    mRNA sequence
    • >TC015170 RA
      taaggcctg ttagatttt taattagaa atttgtgac 
      gcatttacc caaaaaaca gtcagactg tagtaaatt 
      gcggtcgcc acgacccaa attaaacct tcaaacttc 
      ggcaataca taacaaaat aattacttc agagataac 
      ggaccgctc acacaaaaa ccatttcca taacatgac 
      ttttattca ctctgacct tgcggttaa tcggaactc 
      tagagcaca gtaaatacc gtaatctcg cgataatta 
      cataaataa gagacagcg accagattt tccacaatc 
      gtgccagtg attttcacg caaaatgtc gcgaaaaat 
      cggaattgt gggcagcgg ccttatcgg ccgctcgtg 
      ggccatgct gttcgccaa tgtgggcta ccaggtcgt 
      catctacga cattgagcc caaacagat cgaatcggc 
      gttagccga catcgagca acagctcaa aaacttgga 
      aaaaagcgg gcttttacg cgggaaatt gaactcaag 
      ccagcaatt tgcctgcat taaagggac caacagtct 
      aacggaggc cgtaaaaga cgccgtttt ggtccaaga 
      atgcgtccc cgaaaacct ggaactgaa gcgaaaagt 
      gtggaaaaa cgtggacga cgtcagcgg ccctaacac 
      gattttctc aagctcgac atcaacctt cctcccttc 
      gctattctc cgaccatct caaaaacaa gaaaaacat 
      cgtggtgtc gcacccggt gaacccccc gtattacgt 
      gcccttggt cgagatcgt gccagcgcc ctggactga 
      ccccgcagt caccaaaaa aacgcgcgc tattatgga 
      ggaaatcgg gcaaactcc cgtctcact gactaagga 
      agtccccgg ttttgttgt aaatcgcct acagtacgc 
      ccttttgaa cgaaacgtg gaaccttgt ctccgacgg 
      agtcctcga tgtcaaaga catcgacaa ggtcatgtc 
      cgacgggct cggcatgcg ctacgcctt tctgggccc 
      ccttgaaac cactcacct taacgccga gggcttcgt 
      cgactattg caagaaata ttcaaacac gatttattc 
      agtcagcaa ggactttaa accgttgca aaagtggga 
      gggccccct tgtgcagga aatcgccaa acagttgga 
      gaattcggt cccgttgga taagttaca ggaacgcag 
      gaattggcg ggacttggc tctgaccaa actgagcca 
      attgaaaaa atcaatata gcacgtcgt attcaaacg 
      tttatt