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Gene information
General information
TC015165 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2130241..2131458
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015165
    iBeetle screen iB_02416

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands partially slightlycolour darker - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015165 RA
      MAAALSYED LRKHARYLE NEIDLKLVA 
      FSKLGAGIK SPPAHSSSD AVPLLSGED 
      TFEAMSLEI EELLNKLTQ VNDRMGEQP 
      VSGAAMLHT LQRHRDILA DLSRDFRKT 
      TSQHEIRRE REDLLRGSS DTFRGDGVN 
      NRRDMYLKE NQHLHSSER LVNEQISIA 
      METREHLTN QRQTFKRLQ TRFNDLSNR 
      YPVINSLIQ RINIKKRRD SIILGLVVS 
      GCTVLMLLY AFH
    CDS sequence
    • >TC015165 RA
      atggcggcg gctctttca tacgaagac ctccgcaaa 
      cacgcgcgc tacttagag aacgaaatc gacttaaaa 
      ctcgtcgcg tttagtaaa ctgggggcc ggtatcaag 
      tcgccccca gctcattcg agctccgat gctgtgccc 
      cttctctca ggcgaggac actttcgag gccatgtct 
      ctcgaaatt gaggaattg ttgaataaa ttaacacaa 
      gttaacgac aggatgggg gaacagccc gtctcgggg 
      gctgccatg ctgcacacc ctccaacgc caccgtgat 
      attttggcc gatttatcg cgcgatttc cgaaagact 
      acgtcccag cacgagatc aggcgagag agggaggat 
      ttgttaagg gggtccagt gatacgttc aggggcgat 
      ggtgttaat aataggcgc gatatgtac ttgaaggag 
      aatcaacac ttgcacagt tcagagcgg ttggttaat 
      gagcaaatt tccattgcg atggagact cgggaacat 
      ttgaccaat cagagacag acgtttaag cgcttgcag 
      acgaggttt aatgatttg tcaaatcgg tacccagtc 
      atcaatagt ctcattcag aggattaac attaaaaaa 
      aggagggat tcgattatt ttagggttg gtcgtttca 
      gggtgcacc gttttgatg ctactttac gctttccat 
      tga
    mRNA sequence
    • >TC015165 RA
      ttgctcgta tgcgccgtg cggttggct gcacttttt 
      ccaaatcag cagcaacat ggcggcggc tctttcata 
      cgaagacct ccgcaaaca cgcgcgcta cttagagaa 
      cgaaatcga cttaaaact cgtcgcgtt tagtaaact 
      gggggccgg tatcaagtc gcccccagc tcattcgag 
      ctccgatgc tgtgcccct tctctcagg cgaggacac 
      tttcgaggc catgtctct cgaaattga ggaattgtt 
      gaataaatt aacacaagt taacgacag gatggggga 
      acagcccgt ctcgggggc tgccatgct gcacaccct 
      ccaacgcca ccgtgatat tttggccga tttatcgcg 
      cgatttccg aaagactac gtcccagca cgagatcag 
      gcgagagag ggaggattt gttaagggg gtccagtga 
      tacgttcag gggcgatgg tgttaataa taggcgcga 
      tatgtactt gaaggagaa tcaacactt gcacagttc 
      agagcggtt ggttaatga gcaaatttc cattgcgat 
      ggagactcg ggaacattt gaccaatca gagacagac 
      gtttaagcg cttgcagac gaggtttaa tgatttgtc 
      aaatcggta cccagtcat caatagtct cattcagag 
      gattaacat taaaaaaag gagggattc gattatttt 
      agggttggt cgtttcagg gtgcaccgt tttgatgct 
      actttacgc tttccattg actctaact gtgataatt 
      tttattgta tcagaatat ctaaccaaa aatcgaggg 
      tagtgttag tggcggtat ttatatttg ttatgaata 
      aaatgattt ttttaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY