Status
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Gene information
General information
TC034732 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2323819..2344468
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034732
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02414

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment partially number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034732 RA
      MSQNSSAGK RHSSIQLRE RKQKKLYSE DWALGDDEI 
      EGRRSFNLQ EKLENPLFA THNIVKEMH GNELNVAYF 
      QKHGFNTPL LFKEKTGLG LRVPTSNFT INDVRMCVG 
      SRRILDVMD VNTQKNSEM TMKEWQKYY EDPAKDKLL 
      NVISLEFSH TKLENYVQS PTVVRQVDW VDCVWPRHL 
      KESQTEATN LLEDMKYPK VQKYCLMSV KGCYTDFHV 
      DFGGTSVWY HILKGSKVF WLIPPTEHN LALYERWVL 
      SGKQSDIFF GDTVDKCAR IVLTEGNTF FIPTGWIHA 
      VYTPMDSLV FGGNFLHSY GIDKQLKIA HVEDTTKVP 
      QKFRYPFFT EMLWYVLER YVHCLLGNS HLTTGHDVA 
      IPPDRPHVH LTQAELHGL KEIVMFLHM LPANKKNVP 
      DLIRDPVSL IQDVRTLIG HHRQDSREL AITGRPVLW 
      LPDDHDRKS LYPRPYAKS NGVRPPKPA FKSKSGLGG 
      GAGDKGGPR RRRTRCKKC EACQRSDCG ECSFCLDMV 
      KFGGPGRAK QTCNMRQCL QPMLPVTAA CVHCGLDGW 
      NQTPVTPLQ KGPQRCESA SNLMECSVC YEIAHPSCV 
      QRLCPDFTG YINEDMPNS WECPMCCKL GKNTDYRPR 
      HFRARQKSS DMRRMSISS DASSAFDSK LHQEHYSDS 
      GSENDNKDN NKDLLPIKK RRSSEGTPE IETSKSQAS 
      DLPRKTAFR MQLAHQIAS NSTKMLKKP MVVVRPAPI 
      LMMNPLPGN NLALDKRCV LNIFKYLSP KDLLNCALV 
      CKTWATYSI DPTLWRVMA FSRKHISSD ILKGIVRRQ 
      PEVLRLDWC HINKFQLPW LIQRLSNLK ELSLVSVNV 
      KTAISLRTN HSAVLQHLD LSFISDFND AALREILGP 
      NNDSRRGLA DEKIRFRNL RTLKLAGTD VTDIAMRYV 
      TQYLPNLQH LSLSLCPRI SDAGIAQLT TKPATTVGS 
      LVSLDLSHS KLVTETSLE HLAKCESLV RLDCRHSLQ 
      ISTQALIKF AAKSDKNLQ VRDIKLVDK RLKA
    CDS sequence
    • >TC034732 RA
      atgtcgcag aattcgtct gccggcaaa cgacactcc 
      tccatacag ctgagagaa cgcaaacag aagaaactt 
      tactcggag gattgggcg ctcggagat gacgagatc 
      gagggccga aggtcgttc aatctccag gaaaaactg 
      gagaatccg ctcttcgcc acgcacaac atcgtcaag 
      gaaatgcac gggaatgag ctgaatgtg gcgtatttt 
      caaaaacat gggttcaat acgcccctg ctctttaaa 
      gagaagacc ggattgggt ttgcgcgtc ccgacgtcg 
      aacttcacg atcaacgac gtccgcatg tgcgtcggt 
      tcgcgtcgc atcctcgac gtaatggac gtcaacacg 
      caaaaaaac agcgaaatg acaatgaaa gaatggcaa 
      aagtactac gaagacccc gccaaagac aaactcctc 
      aacgtcatc tcgctcgaa ttctcgcac acaaaactc 
      gaaaactac gtccaaagc cccacagtc gtccgtcaa 
      gtcgactgg gtggactgt gtttggcca cgccacctc 
      aaagaaagc caaaccgag gccaccaac ctcctcgaa 
      gacatgaag taccccaaa gtgcaaaag tactgcctc 
      atgtcggtc aagggctgc tacacggac ttccacgtg 
      gattttggc gggacctcc gtctggtac cacatcctc 
      aaaggctcc aaggtcttc tggctcatc ccgcctact 
      gagcacaac ctggctctg tacgaacgg tgggtgctt 
      tcgggcaaa cagtcggat atttttttc ggagatacg 
      gtggataag tgcgcgagg attgtcctc acggagggt 
      aacacgttt tttataccg actgggtgg atacatgcg 
      gtttatacg ccgatggat tcgctggtt ttcggtggg 
      aatttttta cattcgtat ggaatcgat aaacagttg 
      aagatcgcg catgttgag gataccacc aaagtgccg 
      caaaagttt agatatccg ttttttacc gaaatgttg 
      tggtatgtg ctcgaacgg tacgtgcat tgtctctta 
      gggaattca catctgacg acgggacac gatgtggcg 
      ataccgcca gatcggccc catgtgcat ctgacgcag 
      gcggagctg cacggactg aaggaaatc gtcatgttc 
      ctgcacatg ctccccgcc aacaagaag aacgtcccc 
      gacctgatc cgcgacccc gtgtccctc atccaggac 
      gtgcgcacc ctcatcggc caccaccgg caggacagc 
      cgagagctg gccatcacc ggccgtccc gtcctctgg 
      ctgcccgac gaccacgat cgcaagtcc ctctacccg 
      cgtccgtac gcaaaatcg aacggcgtt cggccgccg 
      aagccggca ttcaagtcg aagtcgggc ctcggcggc 
      ggcgccggc gacaaaggc ggcccgcgc cgccgccgc 
      acccgctgc aagaagtgc gaagcgtgc cagaggagc 
      gactgcggc gagtgcagt ttctgtctg gatatggtc 
      aagttcggg gggcccgga agggccaaa cagacgtgc 
      aatatgcgg cagtgtctg cagccgatg ctgccggtg 
      acggccgct tgtgtgcac tgcggcttg gacggctgg 
      aatcagacg ccggtgacg ccgctgcag aaggggccg 
      cagcggtgc gagagtgcc agcaatctt atggagtgt 
      tcggtttgc tacgaaatc gcgcacccc tcgtgcgtg 
      caacgcctc tgccccgac ttcaccggc tacataaac 
      gaggacatg cccaactcg tgggaatgc cccatgtgt 
      tgcaaactg ggcaagaac accgactac cgacctcgc 
      cacttccgc gcccgccaa aaatccagc gacatgcgt 
      cgaatgtcc ataagttcg gacgcttcc agcgcattc 
      gactccaag ctccaccag gagcactac tcggactcg 
      ggcagcgaa aacgacaac aaggacaat aacaaggat 
      ctcctcccg attaagaag cgccgcagc agcgaggga 
      acccccgaa atcgagacg agcaaaagc caagcgagc 
      gatttaccc cgaaaaacg gcgttccgc atgcaactc 
      gcccaccaa atcgcgagc aattcgacg aaaatgttg 
      aagaagccg atggtggtg gtgcgaccg gcgcccatt 
      ttgatgatg aatccgctg ccggggaat aacctagcg 
      ttggataaa cgctgcgtt ttgaatatt ttcaagtat 
      ttgagtccg aaggacttg ttgaattgt gcgctggtt 
      tgtaagact tgggccacg tattccatt gatccgacg 
      ctgtggcgg gtcatggcc ttcagtcgg aagcacatt 
      tcgtccgat attctcaaa ggaatcgtc cggaggcag 
      ccagaagtg ctgagactg gactggtgc cacatcaac 
      aaattccag ctcccgtgg ctcatccag cgcctcagc 
      aacctcaaa gaactgtcc ctagtgagt gtcaacgtc 
      aagacagcc atctccctg cgcaccaac cactcggcc 
      gtcttacaa cacttagat ctcagtttc atctcagat 
      ttcaacgat gccgcactt agagaaatc ctcggcccg 
      aacaacgac tcgcggcgc ggtttagcc gacgagaaa 
      atacgcttt cgcaacttg cggacgctt aaactcgca 
      ggaaccgac gttaccgac attgccatg cggtacgtg 
      actcagtat ttgcccaat ttgcagcat ctcagtctg 
      agtctgtgt ccgcggatt agcgatgcc gggattgcg 
      caattaacg actaagccg gcaacgacc gttgggagt 
      ttggttagt cttgatctg agccactcg aaactggtg 
      actgagacg agtttggaa catttggcc aagtgtgag 
      agtttagtg cggttggac tgtagacac tcgttgcag 
      atttcgacg caagcactg atcaagttt gccgcgaaa 
      agtgataag aacttgcaa gtgagggac attaagttg 
      gttgataag aggttgaag gcgtga
    mRNA sequence
    • >TC034732 RA
      gcgcttggt gtggttgtg tttgcgtta aatttgcaa 
      aataattta cgaatcgta cgtaaaatc gcgagtgaa 
      tttcgttga aatgtcgca gaattcgtc tgccggcaa 
      acgacactc ctccataca gctgagaga acgcaaaca 
      gaagaaact ttactcgga ggattgggc gctcggaga 
      tgacgagat cgagggccg aaggtcgtt caatctcca 
      ggaaaaact ggagaatcc gctcttcgc cacgcacaa 
      catcgtcaa ggaaatgca cgggaatga gctgaatgt 
      ggcgtattt tcaaaaaca tgggttcaa tacgcccct 
      gctctttaa agagaagac cggattggg tttgcgcgt 
      cccgacgtc gaacttcac gatcaacga cgtccgcat 
      gtgcgtcgg ttcgcgtcg catcctcga cgtaatgga 
      cgtcaacac gcaaaaaaa cagcgaaat gacaatgaa 
      agaatggca aaagtacta cgaagaccc cgccaaaga 
      caaactcct caacgtcat ctcgctcga attctcgca 
      cacaaaact cgaaaacta cgtccaaag ccccacagt 
      cgtccgtca agtcgactg ggtggactg tgtttggcc 
      acgccacct caaagaaag ccaaaccga ggccaccaa 
      cctcctcga agacatgaa gtaccccaa agtgcaaaa 
      gtactgcct catgtcggt caagggctg ctacacgga 
      cttccacgt ggattttgg cgggacctc cgtctggta 
      ccacatcct caaaggctc caaggtctt ctggctcat 
      cccgcctac tgagcacaa cctggctct gtacgaacg 
      gtgggtgct ttcgggcaa acagtcgga tattttttt 
      cggagatac ggtggataa gtgcgcgag gattgtcct 
      cacggaggg taacacgtt ttttatacc gactgggtg 
      gatacatgc ggtttatac gccgatgga ttcgctggt 
      tttcggtgg gaatttttt acattcgta tggaatcga 
      taaacagtt gaagatcgc gcatgttga ggataccac 
      caaagtgcc gcaaaagtt tagatatcc gttttttac 
      cgaaatgtt gtggtatgt gctcgaacg gtacgtgca 
      ttgtctctt agggaattc acatctgac gacgggaca 
      cgatgtggc gataccgcc agatcggcc ccatgtgca 
      tctgacgca ggcggagct gcacggact gaaggaaat 
      cgtcatgtt cctgcacat gctccccgc caacaagaa 
      gaacgtccc cgacctgat ccgcgaccc cgtgtccct 
      catccagga cgtgcgcac cctcatcgg ccaccaccg 
      gcaggacag ccgagagct ggccatcac cggccgtcc 
      cgtcctctg gctgcccga cgaccacga tcgcaagtc 
      cctctaccc gcgtccgta cgcaaaatc gaacggcgt 
      tcggccgcc gaagccggc attcaagtc gaagtcggg 
      cctcggcgg cggcgccgg cgacaaagg cggcccgcg 
      ccgccgccg cacccgctg caagaagtg cgaagcgtg 
      ccagaggag cgactgcgg cgagtgcag tttctgtct 
      ggatatggt caagttcgg ggggcccgg aagggccaa 
      acagacgtg caatatgcg gcagtgtct gcagccgat 
      gctgccggt gacggccgc ttgtgtgca ctgcggctt 
      ggacggctg gaatcagac gccggtgac gccgctgca 
      gaaggggcc gcagcggtg cgagagtgc cagcaatct 
      tatggagtg ttcggtttg ctacgaaat cgcgcaccc 
      ctcgtgcgt gcaacgcct ctgccccga cttcaccgg 
      ctacataaa cgaggacat gcccaactc gtgggaatg 
      ccccatgtg ttgcaaact gggcaagaa caccgacta 
      ccgacctcg ccacttccg cgcccgcca aaaatccag 
      cgacatgcg tcgaatgtc cataagttc ggacgcttc 
      cagcgcatt cgactccaa gctccacca ggagcacta 
      ctcggactc gggcagcga aaacgacaa caaggacaa 
      taacaagga tctcctccc gattaagaa gcgccgcag 
      cagcgaggg aacccccga aatcgagac gagcaaaag 
      ccaagcgag cgatttacc ccgaaaaac ggcgttccg 
      catgcaact cgcccacca aatcgcgag caattcgac 
      gaaaatgtt gaagaagcc gatggtggt ggtgcgacc 
      ggcgcccat tttgatgat gaatccgct gccggggaa 
      taacctagc gttggataa acgctgcgt tttgaatat 
      tttcaagta tttgagtcc gaaggactt gttgaattg 
      tgcgctggt ttgtaagac ttgggccac gtattccat 
      tgatccgac gctgtggcg ggtcatggc cttcagtcg 
      gaagcacat ttcgtccga tattctcaa aggaatcgt 
      ccggaggca gccagaagt gctgagact ggactggtg 
      ccacatcaa caaattcca gctcccgtg gctcatcca 
      gcgcctcag caacctcaa agaactgtc cctagtgag 
      tgtcaacgt caagacagc catctccct gcgcaccaa 
      ccactcggc cgtcttaca acacttaga tctcagttt 
      catctcaga tttcaacga tgccgcact tagagaaat 
      cctcggccc gaacaacga ctcgcggcg cggtttagc 
      cgacgagaa aatacgctt tcgcaactt gcggacgct 
      taaactcgc aggaaccga cgttaccga cattgccat 
      gcggtacgt gactcagta tttgcccaa tttgcagca 
      tctcagtct gagtctgtg tccgcggat tagcgatgc 
      cgggattgc gcaattaac gactaagcc ggcaacgac 
      cgttgggag tttggttag tcttgatct gagccactc 
      gaaactggt gactgagac gagtttgga acatttggc 
      caagtgtga gagtttagt gcggttgga ctgtagaca 
      ctcgttgca gatttcgac gcaagcact gatcaagtt 
      tgccgcgaa aagtgataa gaacttgca agtgaggga 
      cattaagtt ggttgataa gaggttgaa ggcgtgact 
      gatacgtaa tttatttca ataggcttg atgtgtgag 
      gattgtgga tgtgtatat agtattatt ctttgatat 
      aagtgattt gtatgagac atagcggaa aatacaatt 
      ttttatatt at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY