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Gene information
General information
TC034732 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 1367595..1388244
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034732
    iBeetle screen iB_02414
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment partially number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034732 RA
      MSQNSSAGK RHSSIQLRE RKQKKLYSE DWALGDDEI 
EGRRSFNLQ EKLENPLFA THNIVKEMH GNELNVAYF 
QKHGFNTPL LFKEKTGLG LRVPTSNFT INDVRMCVG 
SRRILDVMD VNTQKNSEM TMKEWQKYY EDPAKDKLL 
NVISLEFSH TKLENYVQS PTVVRQVDW VDCVWPRHL 
KESQTEATN LLEDMKYPK VQKYCLMSV KGCYTDFHV 
DFGGTSVWY HILKGSKVF WLIPPTEHN LALYERWVL 
SGKQSDIFF GDTVDKCAR IVLTEGNTF FIPTGWIHA 
VYTPMDSLV FGGNFLHSY GIDKQLKIA HVEDTTKVP 
QKFRYPFFT EMLWYVLER YVHCLLGNS HLTTGHDVA 
IPPDRPHVH LTQAELHGL KEIVMFLHM LPANKKNVP 
DLIRDPVSL IQDVRTLIG HHRQDSREL AITGRPVLW 
LPDDHDRKS LYPRPYAKS NGVRPPKPA FKSKSGLGG 
GAGDKGGPR RRRTRCKKC EACQRSDCG ECSFCLDMV 
KFGGPGRAK QTCNMRQCL QPMLPVTAA CVHCGLDGW 
NQTPVTPLQ KGPQRCESA SNLMECSVC YEIAHPSCV 
QRLCPDFTG YINEDMPNS WECPMCCKL GKNTDYRPR 
HFRARQKSS DMRRMSISS DASSAFDSK LHQEHYSDS 
GSENDNKDN NKDLLPIKK RRSSEGTPE IETSKSQAS 
DLPRKTAFR MQLAHQIAS NSTKMLKKP MVVVRPAPI 
LMMNPLPGN NLALDKRCV LNIFKYLSP KDLLNCALV 
CKTWATYSI DPTLWRVMA FSRKHISSD ILKGIVRRQ 
PEVLRLDWC HINKFQLPW LIQRLSNLK ELSLVSVNV 
KTAISLRTN HSAVLQHLD LSFISDFND AALREILGP 
NNDSRRGLA DEKIRFRNL RTLKLAGTD VTDIAMRYV 
TQYLPNLQH LSLSLCPRI SDAGIAQLT TKPATTVGS 
LVSLDLSHS KLVTETSLE HLAKCESLV RLDCRHSLQ 
ISTQALIKF AAKSDKNLQ VRDIKLVDK RLKA
    CDS sequence
    • >TC034732 RA
      atgtcgcag aattcgtct gccggcaaa cgacactcc 
tccatacag ctgagagaa cgcaaacag aagaaactt 
tactcggag gattgggcg ctcggagat gacgagatc 
gagggccga aggtcgttc aatctccag gaaaaactg 
gagaatccg ctcttcgcc acgcacaac atcgtcaag 
gaaatgcac gggaatgag ctgaatgtg gcgtatttt 
caaaaacat gggttcaat acgcccctg ctctttaaa 
gagaagacc ggattgggt ttgcgcgtc ccgacgtcg 
aacttcacg atcaacgac gtccgcatg tgcgtcggt 
tcgcgtcgc atcctcgac gtaatggac gtcaacacg 
caaaaaaac agcgaaatg acaatgaaa gaatggcaa 
aagtactac gaagacccc gccaaagac aaactcctc 
aacgtcatc tcgctcgaa ttctcgcac acaaaactc 
gaaaactac gtccaaagc cccacagtc gtccgtcaa 
gtcgactgg gtggactgt gtttggcca cgccacctc 
aaagaaagc caaaccgag gccaccaac ctcctcgaa 
gacatgaag taccccaaa gtgcaaaag tactgcctc 
atgtcggtc aagggctgc tacacggac ttccacgtg 
gattttggc gggacctcc gtctggtac cacatcctc 
aaaggctcc aaggtcttc tggctcatc ccgcctact 
gagcacaac ctggctctg tacgaacgg tgggtgctt 
tcgggcaaa cagtcggat atttttttc ggagatacg 
gtggataag tgcgcgagg attgtcctc acggagggt 
aacacgttt tttataccg actgggtgg atacatgcg 
gtttatacg ccgatggat tcgctggtt ttcggtggg 
aatttttta cattcgtat ggaatcgat aaacagttg 
aagatcgcg catgttgag gataccacc aaagtgccg 
caaaagttt agatatccg ttttttacc gaaatgttg 
tggtatgtg ctcgaacgg tacgtgcat tgtctctta 
gggaattca catctgacg acgggacac gatgtggcg 
ataccgcca gatcggccc catgtgcat ctgacgcag 
gcggagctg cacggactg aaggaaatc gtcatgttc 
ctgcacatg ctccccgcc aacaagaag aacgtcccc 
gacctgatc cgcgacccc gtgtccctc atccaggac 
gtgcgcacc ctcatcggc caccaccgg caggacagc 
cgagagctg gccatcacc ggccgtccc gtcctctgg 
ctgcccgac gaccacgat cgcaagtcc ctctacccg 
cgtccgtac gcaaaatcg aacggcgtt cggccgccg 
aagccggca ttcaagtcg aagtcgggc ctcggcggc 
ggcgccggc gacaaaggc ggcccgcgc cgccgccgc 
acccgctgc aagaagtgc gaagcgtgc cagaggagc 
gactgcggc gagtgcagt ttctgtctg gatatggtc 
aagttcggg gggcccgga agggccaaa cagacgtgc 
aatatgcgg cagtgtctg cagccgatg ctgccggtg 
acggccgct tgtgtgcac tgcggcttg gacggctgg 
aatcagacg ccggtgacg ccgctgcag aaggggccg 
cagcggtgc gagagtgcc agcaatctt atggagtgt 
tcggtttgc tacgaaatc gcgcacccc tcgtgcgtg 
caacgcctc tgccccgac ttcaccggc tacataaac 
gaggacatg cccaactcg tgggaatgc cccatgtgt 
tgcaaactg ggcaagaac accgactac cgacctcgc 
cacttccgc gcccgccaa aaatccagc gacatgcgt 
cgaatgtcc ataagttcg gacgcttcc agcgcattc 
gactccaag ctccaccag gagcactac tcggactcg 
ggcagcgaa aacgacaac aaggacaat aacaaggat 
ctcctcccg attaagaag cgccgcagc agcgaggga 
acccccgaa atcgagacg agcaaaagc caagcgagc 
gatttaccc cgaaaaacg gcgttccgc atgcaactc 
gcccaccaa atcgcgagc aattcgacg aaaatgttg 
aagaagccg atggtggtg gtgcgaccg gcgcccatt 
ttgatgatg aatccgctg ccggggaat aacctagcg 
ttggataaa cgctgcgtt ttgaatatt ttcaagtat 
ttgagtccg aaggacttg ttgaattgt gcgctggtt 
tgtaagact tgggccacg tattccatt gatccgacg 
ctgtggcgg gtcatggcc ttcagtcgg aagcacatt 
tcgtccgat attctcaaa ggaatcgtc cggaggcag 
ccagaagtg ctgagactg gactggtgc cacatcaac 
aaattccag ctcccgtgg ctcatccag cgcctcagc 
aacctcaaa gaactgtcc ctagtgagt gtcaacgtc 
aagacagcc atctccctg cgcaccaac cactcggcc 
gtcttacaa cacttagat ctcagtttc atctcagat 
ttcaacgat gccgcactt agagaaatc ctcggcccg 
aacaacgac tcgcggcgc ggtttagcc gacgagaaa 
atacgcttt cgcaacttg cggacgctt aaactcgca 
ggaaccgac gttaccgac attgccatg cggtacgtg 
actcagtat ttgcccaat ttgcagcat ctcagtctg 
agtctgtgt ccgcggatt agcgatgcc gggattgcg 
caattaacg actaagccg gcaacgacc gttgggagt 
ttggttagt cttgatctg agccactcg aaactggtg 
actgagacg agtttggaa catttggcc aagtgtgag 
agtttagtg cggttggac tgtagacac tcgttgcag 
atttcgacg caagcactg atcaagttt gccgcgaaa 
agtgataag aacttgcaa gtgagggac attaagttg 
gttgataag aggttgaag gcgtga
    mRNA sequence
    • >TC034732 RA
      gcgcttggt gtggttgtg tttgcgtta aatttgcaa 
aataattta cgaatcgta cgtaaaatc gcgagtgaa 
tttcgttga aatgtcgca gaattcgtc tgccggcaa 
acgacactc ctccataca gctgagaga acgcaaaca 
gaagaaact ttactcgga ggattgggc gctcggaga 
tgacgagat cgagggccg aaggtcgtt caatctcca 
ggaaaaact ggagaatcc gctcttcgc cacgcacaa 
catcgtcaa ggaaatgca cgggaatga gctgaatgt 
ggcgtattt tcaaaaaca tgggttcaa tacgcccct 
gctctttaa agagaagac cggattggg tttgcgcgt 
cccgacgtc gaacttcac gatcaacga cgtccgcat 
gtgcgtcgg ttcgcgtcg catcctcga cgtaatgga 
cgtcaacac gcaaaaaaa cagcgaaat gacaatgaa 
agaatggca aaagtacta cgaagaccc cgccaaaga 
caaactcct caacgtcat ctcgctcga attctcgca 
cacaaaact cgaaaacta cgtccaaag ccccacagt 
cgtccgtca agtcgactg ggtggactg tgtttggcc 
acgccacct caaagaaag ccaaaccga ggccaccaa 
cctcctcga agacatgaa gtaccccaa agtgcaaaa 
gtactgcct catgtcggt caagggctg ctacacgga 
cttccacgt ggattttgg cgggacctc cgtctggta 
ccacatcct caaaggctc caaggtctt ctggctcat 
cccgcctac tgagcacaa cctggctct gtacgaacg 
gtgggtgct ttcgggcaa acagtcgga tattttttt 
cggagatac ggtggataa gtgcgcgag gattgtcct 
cacggaggg taacacgtt ttttatacc gactgggtg 
gatacatgc ggtttatac gccgatgga ttcgctggt 
tttcggtgg gaatttttt acattcgta tggaatcga 
taaacagtt gaagatcgc gcatgttga ggataccac 
caaagtgcc gcaaaagtt tagatatcc gttttttac 
cgaaatgtt gtggtatgt gctcgaacg gtacgtgca 
ttgtctctt agggaattc acatctgac gacgggaca 
cgatgtggc gataccgcc agatcggcc ccatgtgca 
tctgacgca ggcggagct gcacggact gaaggaaat 
cgtcatgtt cctgcacat gctccccgc caacaagaa 
gaacgtccc cgacctgat ccgcgaccc cgtgtccct 
catccagga cgtgcgcac cctcatcgg ccaccaccg 
gcaggacag ccgagagct ggccatcac cggccgtcc 
cgtcctctg gctgcccga cgaccacga tcgcaagtc 
cctctaccc gcgtccgta cgcaaaatc gaacggcgt 
tcggccgcc gaagccggc attcaagtc gaagtcggg 
cctcggcgg cggcgccgg cgacaaagg cggcccgcg 
ccgccgccg cacccgctg caagaagtg cgaagcgtg 
ccagaggag cgactgcgg cgagtgcag tttctgtct 
ggatatggt caagttcgg ggggcccgg aagggccaa 
acagacgtg caatatgcg gcagtgtct gcagccgat 
gctgccggt gacggccgc ttgtgtgca ctgcggctt 
ggacggctg gaatcagac gccggtgac gccgctgca 
gaaggggcc gcagcggtg cgagagtgc cagcaatct 
tatggagtg ttcggtttg ctacgaaat cgcgcaccc 
ctcgtgcgt gcaacgcct ctgccccga cttcaccgg 
ctacataaa cgaggacat gcccaactc gtgggaatg 
ccccatgtg ttgcaaact gggcaagaa caccgacta 
ccgacctcg ccacttccg cgcccgcca aaaatccag 
cgacatgcg tcgaatgtc cataagttc ggacgcttc 
cagcgcatt cgactccaa gctccacca ggagcacta 
ctcggactc gggcagcga aaacgacaa caaggacaa 
taacaagga tctcctccc gattaagaa gcgccgcag 
cagcgaggg aacccccga aatcgagac gagcaaaag 
ccaagcgag cgatttacc ccgaaaaac ggcgttccg 
catgcaact cgcccacca aatcgcgag caattcgac 
gaaaatgtt gaagaagcc gatggtggt ggtgcgacc 
ggcgcccat tttgatgat gaatccgct gccggggaa 
taacctagc gttggataa acgctgcgt tttgaatat 
tttcaagta tttgagtcc gaaggactt gttgaattg 
tgcgctggt ttgtaagac ttgggccac gtattccat 
tgatccgac gctgtggcg ggtcatggc cttcagtcg 
gaagcacat ttcgtccga tattctcaa aggaatcgt 
ccggaggca gccagaagt gctgagact ggactggtg 
ccacatcaa caaattcca gctcccgtg gctcatcca 
gcgcctcag caacctcaa agaactgtc cctagtgag 
tgtcaacgt caagacagc catctccct gcgcaccaa 
ccactcggc cgtcttaca acacttaga tctcagttt 
catctcaga tttcaacga tgccgcact tagagaaat 
cctcggccc gaacaacga ctcgcggcg cggtttagc 
cgacgagaa aatacgctt tcgcaactt gcggacgct 
taaactcgc aggaaccga cgttaccga cattgccat 
gcggtacgt gactcagta tttgcccaa tttgcagca 
tctcagtct gagtctgtg tccgcggat tagcgatgc 
cgggattgc gcaattaac gactaagcc ggcaacgac 
cgttgggag tttggttag tcttgatct gagccactc 
gaaactggt gactgagac gagtttgga acatttggc 
caagtgtga gagtttagt gcggttgga ctgtagaca 
ctcgttgca gatttcgac gcaagcact gatcaagtt 
tgccgcgaa aagtgataa gaacttgca agtgaggga 
cattaagtt ggttgataa gaggttgaa ggcgtgact 
gatacgtaa tttatttca ataggcttg atgtgtgag 
gattgtgga tgtgtatat agtattatt ctttgatat 
aagtgattt gtatgagac atagcggaa aatacaatt 
ttttatatt at