Status
Please wait ...
Gene information
General information
TC015134 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2797841..2806047
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015134
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02411

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber not present - Number of animals showing the phenotype: 4

    vitellarium degenerated - Number of animals showing the phenotype: 4

    tropharium structure irregular - Number of animals showing the phenotype: 4

    Comment: Potentially yolk deposition observed in tropharium.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015134 RA
      MASKSKRST TPASQSVPA RPSSPLSPT RHSRMQEKA 
      DLQNLNDRL AIYIDRVRY LEGENSRLT REVQKTHES 
      VTRQVTDIK SMYDSELTE ARKLLDESF REKAKLEID 
      TKRLWDENE ELKAELAKK SKELVLAEN SARICETRC 
      NELQIKFNQ ANADAKKAL SGLKDLEKE RDKLRKQCE 
      ELRKQLEEE SLARVDVEN HNQTLREEL SFKDQVFQK 
      EISDTRTLR QVEISEIDG RLAEKYEAK LQEALQELR 
      DQYEGQMAS NRQEIEMLY ESKIKNLQD AANRHSNSA 
      GNALNELRQ VRNRIDTLT SKITELENE NSSLQNRCR 
      DLEKILENE RMRHAEDLA ALENDLARL REEMANQLQ 
      EYQDLMDIK VSLDTEIAA YRKLLESEE ARLNIKPHG 
      SPEPHQVAR GSSQRRTPV RVGAKRKRT MLEESHEQS 
      LSDFSVNSS SKGEIEVAE VDPEGKFVK LHNKSGQEV 
      ALGGWTVTR KAGDAETLY KFHRSLKIE PNGYVTIWS 
      ADQNREHEP PTNLVMKGQ KWMVADNMT TTVCNNNGD 
      EVAVSERVK RQLSSSVLR HREGFLGSG SEELYHQLG 
      DPPSEEKCR IM
    CDS sequence
    • >TC015134 RA
      atggcatcg aaatcgaaa agaagcacg acgccggca 
      tcgcaatcg gtgccggcg cgtccctcc agccccttg 
      agccccacg cgccactcc cgcatgcaa gaaaaggcc 
      gatttacag aacctcaac gaccggctg gccatctac 
      atcgaccgg gtccggtac ctggagggc gaaaacagc 
      cgcctgacc cgcgaggtg caaaagacc cacgagtcc 
      gtcacccgg caagtcacc gacatcaaa tcaatgtac 
      gacagcgag ctcaccgag gcccgcaaa ctcctagac 
      gagtccttc agagagaag gccaagctc gaaattgac 
      acgaagcgg ctctgggac gaaaacgag gaactcaaa 
      gccgagttg gccaaaaag agcaaggag ctggtcctg 
      gccgagaac tcggcccgc atttgcgaa acgaggtgc 
      aacgagctc cagatcaag ttcaaccag gccaatgcc 
      gacgccaag aaggccctc agcggcctc aaagacttg 
      gagaaggag agggacaag ttgcggaag cagtgcgag 
      gagttgagg aagcaactc gaggaggag tcgctagcc 
      cgggttgat gtcgaaaat cataaccaa actttgagg 
      gaggaactc tcgttcaag gatcaggtc ttccagaag 
      gagatctcg gacacgcgc acgctgcgc caggtggaa 
      atcagcgag atcgacggc cgtctcgcc gaaaaatac 
      gaagccaag ctccaggaa gccctccag gagttgcgc 
      gaccaatac gagggccaa atggcaagc aatcgtcaa 
      gagatcgaa atgctctac gagtcaaaa atcaaaaat 
      ctccaagac gccgccaac cgtcattcc aactccgcc 
      ggaaacgcc ctcaacgaa ctgcgccaa gtgcgcaac 
      cgcatcgac accctcacc agcaagata accgaactg 
      gaaaacgaa aattcctcg ttgcaaaat cgctgtcgc 
      gacttggaa aaaatcctc gaaaatgag cgaatgcgt 
      cacgccgag gacttggcc gctttggag aacgacctg 
      gcgcgcctg cgcgaggaa atggccaat cagctgcaa 
      gaataccag gacttgatg gacatcaaa gtgagcctt 
      gacaccgag atcgccgcc taccgcaaa ctgctcgag 
      agcgaagag gcgcgactc aacatcaag ccgcacggc 
      tcgcccgag ccgcatcaa gtcgcccgc ggaagctcc 
      caacgacgg acacccgtc cgcgtgggc gccaagcgc 
      aagcggacg atgctcgag gagagccac gagcagagt 
      ttgagcgac tttagcgtg aacagcagc tccaagggc 
      gagattgag gtggcggaa gtggatccg gagggaaag 
      tttgtcaag cttcataat aagagcggc caagaggtg 
      gcgctcggt ggctggacg gttacacgg aaagctggc 
      gatgcggaa acactttat aagttccat cggtcgttg 
      aaaattgaa ccgaatggg tatgtgacc atttggtcc 
      gccgaccag aacagggaa catgaacca cccacgaat 
      ctggtgatg aagggacag aagtggatg gttgccgat 
      aatatgacc acgactgtt tgcaataat aacggagat 
      gaagtcgcg gtgtctgaa agagttaaa aggcagctc 
      tcgagttcg gttttaagg catagggag ggttttctc 
      ggttctggt tctgaagaa ttgtatcac caactcggg 
      gatccccca agcgaggag aagtgccgg ataatgtga
    mRNA sequence
    • >TC015134 RA
      cacctattt acattttac tgtataaaa ccgttaaac 
      cgcttgtcg gcgactctc ctatcatcg cccaaaaat 
      agtcgtgat agtttgtat caacctcgg cggcatttc 
      cctcaaaaa cctcgcgaa aaatcagtg tgttgtgtt 
      agtgcatag tgtgctatg gcatcgaaa tcgaaaaga 
      agcacgacg ccggcatcg caatcggtg ccggcgcgt 
      ccctccagc cccttgagc cccacgcgc cactcccgc 
      atgcaagaa aaggccgat ttacagaac ctcaacgac 
      cggctggcc atctacatc gaccgggtc cggtacctg 
      gagggcgaa aacagccgc ctgacccgc gaggtgcaa 
      aagacccac gagtccgtc acccggcaa gtcaccgac 
      atcaaatca atgtacgac agcgagctc accgaggcc 
      cgcaaactc ctagacgag tccttcaga gagaaggcc 
      aagctcgaa attgacacg aagcggctc tgggacgaa 
      aacgaggaa ctcaaagcc gagttggcc aaaaagagc 
      aaggagctg gtcctggcc gagaactcg gcccgcatt 
      tgcgaaacg aggtgcaac gagctccag atcaagttc 
      aaccaggcc aatgccgac gccaagaag gccctcagc 
      ggcctcaaa gacttggag aaggagagg gacaagttg 
      cggaagcag tgcgaggag ttgaggaag caactcgag 
      gaggagtcg ctagcccgg gttgatgtc gaaaatcat 
      aaccaaact ttgagggag gaactctcg ttcaaggat 
      caggtcttc cagaaggag atctcggac acgcgcacg 
      ctgcgccag gtggaaatc agcgagatc gacggccgt 
      ctcgccgaa aaatacgaa gccaagctc caggaagcc 
      ctccaggag ttgcgcgac caatacgag ggccaaatg 
      gcaagcaat cgtcaagag atcgaaatg ctctacgag 
      tcaaaaatc aaaaatctc caagacgcc gccaaccgt 
      cattccaac tccgccgga aacgccctc aacgaactg 
      cgccaagtg cgcaaccgc atcgacacc ctcaccagc 
      aagataacc gaactggaa aacgaaaat tcctcgttg 
      caaaatcgc tgtcgcgac ttggaaaaa atcctcgaa 
      aatgagcga atgcgtcac gccgaggac ttggccgct 
      ttggagaac gacctggcg cgcctgcgc gaggaaatg 
      gccaatcag ctgcaagaa taccaggac ttgatggac 
      atcaaagtg agccttgac accgagatc gccgcctac 
      cgcaaactg ctcgagagc gaagaggcg cgactcaac 
      atcaagccg cacggctcg cccgagccg catcaagtc 
      gcccgcgga agctcccaa cgacggaca cccgtccgc 
      gtgggcgcc aagcgcaag cggacgatg ctcgaggag 
      agccacgag cagagtttg agcgacttt agcgtgaac 
      agcagctcc aagggcgag attgaggtg gcggaagtg 
      gatccggag ggaaagttt gtcaagctt cataataag 
      agcggccaa gaggtggcg ctcggtggc tggacggtt 
      acacggaaa gctggcgat gcggaaaca ctttataag 
      ttccatcgg tcgttgaaa attgaaccg aatgggtat 
      gtgaccatt tggtccgcc gaccagaac agggaacat 
      gaaccaccc acgaatctg gtgatgaag ggacagaag 
      tggatggtt gccgataat atgaccacg actgtttgc 
      aataataac ggagatgaa gtcgcggtg tctgaaaga 
      gttaaaagg cagctctcg agttcggtt ttaaggcat 
      agggagggt tttctcggt tctggttct gaagaattg 
      tatcaccaa ctcggggat cccccaagc gaggagaag 
      tgccggata atgtgaaaa gtctaccac tagagggcg 
      aaacggtgc cattttttt ctcccaaac gcgagacta 
      agacggccg tgtttcacg gccaacttt gagattttt 
      atccggctt gtcggacgg ataaacgtg tcgaaatgg 
      ggccgctgt gtttatttt aaggaataa ttgtcttca 
      tagagtttg ttaaagcta gagattttt gatatattt 
      tttaattta tataagtat taggtgacc ttcaaactt 
      ggtgcagtt tgtaagact tttctctta tttttaaac 
      taagcattt ttataccaa ggattgtcg ctcctggtt 
      tagacgtaa gttttggat tccttttgt attttttct 
      gtgttcaaa taaatactt taatctagg gtttttcgt 
      tttttcctg atttgtcaa tttgtgcct tatagtttt 
      gtatttcgt gcatgttcg taagcttta atgtaggtg 
      aaagatttt cacgtttta aaatttaat aaagtatat 
      tttcacca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY