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Gene information
General information
TC015124 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 1724040..1738137
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015124
    iBeetle screen iB_02409
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4
    tropharium irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015124 RA
      MNTVNAGDN FYDFTNEEN SAKWKGVLT TALKRVLEQ 
      VHPSLDARE DALEYVENL CLRLLAMLC AKPSPHTVQ 
      DVEYRVQST FPTPIDKWA LKEAQGALD RGKKKSVLL 
      LPVDKIHSL LQKELLLYK VDSTVSLFL VAVLEYISA 
      DILKLAGNY VKNIKHFEI TYQDVQISM NADKALMDM 
      FYQDEGGGP GLPTESLLV SSAPRTSLT YEEVVRELI 
      ASEKAYIKE LHMLIKVFR EEIIKLSSD STEIELIFS 
      NIMDIYELT YTLLGSLED VIEMAQDQM SYIGSCFEE 
      LAEAAEFDV YIKYAKDVT APTCRSTLN SLLSRPDVE 
      NALISAGQG MRLALKYYL PALLMGPIR HCFSYIEYI 
      TILRGLSVA PEDRETLTQ VEGLLKPLQ IELSRCVPS 
      PAMLRSCST PFLQVRARR QAALDKLHE IEKNVENWD 
      PKDLGQCCN EFVREDVLM KVSSGKRLT ERRVFLFDG 
      LLILCKPNS RRQSSVHQN HAECRLKER FFIRKVEII 
      DHLDTEELK NAFEIAPRH MQPSVILCA KTAEEKNSW 
      MADLVMLNT RSMLERILD SILSDIERK HPLKLPPPS 
      LYKFAEPDS KDNIILEQR ENGGVPLIK GATLYKLVE 
      RLTYHIYAD PKFVRTFLT TYRSFCSPK ELLDLLIER 
      FQIPDPSLV YEQECCDTD KMQKNNQRE DWKKYRKEY 
      VQPVQFRVL NVLRHWVDH HFYDFERDP TLLVKLRDF 
      LDAVNGKSM RKWVDSVLK ILHRKMEND NQRHIRFAF 
      DEPPPPIEW HIPCTEEEY GILTLHPVE IARQLTILE 
      FDLYRMIKP SELVGAVWT KKDKETTSP NLLKMIKHT 
      TNVGRWLEK NIVEAENFE ERVAILNRI IEIMIVLYE 
      INNFNGVLS IVSTCASAS VHRLKLTFA ALTQQNKKA 
      LEECRADDH FKKYQEKLR SINPPCVPF LGMYLTNIL 
      HIEEGNPDF LPNTQLINF FKRRKVAEI TGEIQQYQN 
      QPYCFSVES KIRHFLENL NPFGDLSDT EISNYLYNK 
      SMEIEPRGQ KQVQKFPRK WPHLSLKSP GIKSKAKTT 
      ATAVTNAIN STLNSSNIK SDDSKSEDS SKSDNEFSV 
      FAPVQLGTC QNSPTLSPV SPATSNTIL SWFQPNHNR 
      SPSVGSIMS TTSMRNPPP PPPQASKYQ HYSQSSTGG 
      SNSPGPHSP ASPGPVQSY EPNSPRRPL TPPPPPPPL 
      PPRRRRDSP EISSPQLKT DPHWRECSP PPLPPRRGD 
      PGSSPSSHI QHSHHSFNS FGGGTMPRL NPTHMSQLH 
      MRRHSTVQP QVPRRTVHA NVTPLVPQN QPASISPRY 
      DSATPRLPP RPAARSADM VTGPMFQYP STTTT
    CDS sequence
    • >TC015124 RA
      atgaatact gtcaatgcc ggcgacaac ttttacgat 
      ttcaccaat gaggaaaat tcggccaag tggaaagga 
      gtgctcacc accgccttg aagcgggtg ctggagcag 
      gtgcacccg tctttggac gcccgcgaa gatgccctc 
      gaatacgtc gaaaacctc tgtttgcgc cttctggcc 
      atgctctgc gccaaacct agtccacac acagtccag 
      gatgttgaa tatagagta caaagcacg tttcctacc 
      cccatcgat aagtgggcc ttgaaagag gcccaaggc 
      gccctcgat cgaggcaaa aagaagtcc gttttgctg 
      ttgccagtt gacaaaatt cacagttta ttacaaaag 
      gaattgcta ttgtacaaa gtcgactcg accgtctcc 
      ctctttcta gtcgctgtt ttggagtac atttcggcc 
      gatatttta aaattggcg ggaaattac gtgaaaaac 
      atcaaacat ttcgaaatc acctaccag gatgtccag 
      atttcgatg aatgcggat aaggcactc atggatatg 
      ttctatcaa gatgaagga gggggacct ggtttaccg 
      actgagtcg cttttagtg tcgtcggct ccgcgcacg 
      agtctcacg tatgaggaa gtcgtaaga gagttaata 
      gcttcggaa aaagcatac atcaaagaa ctacacatg 
      ttgatcaaa gtgtttcgc gaggaaatt atcaaacta 
      agctccgat tcgaccgaa attgaactg attttttcg 
      aacattatg gatatttac gaactgacg tatactcta 
      ttgggtagt ttggaggac gtcatcgag atggcgcaa 
      gaccaaatg tcgtacatt ggcagctgt ttcgaagag 
      ctggccgaa gccgccgaa ttcgacgtg tacataaaa 
      tacgcgaaa gacgtaacc gcccccaca tgtcgctcc 
      accctcaac agcctcctc tcgcgcccc gacgtcgaa 
      aacgcgctc atctcagcc ggtcaaggc atgcgtctc 
      gccctcaag tattacctg cctgcgctt ctaatgggc 
      cccatccga cactgcttc tcctacatc gaatacatc 
      accatttta cggggcctg tccgtggcc cccgaagac 
      cgcgaaacc ctcacccaa gtcgaaggc ttactcaaa 
      cccctccaa atcgagctc tcccggtgc gtcccctcc 
      cccgccatg ctgcgcagc tgctccacc cccttcctc 
      caagtgcgg gcgcgccga caagccgcc ttggataaa 
      ctgcacgaa atcgagaag aacgtcgaa aattgggac 
      ccgaaggat ttgggacag tgttgcaac gagttcgtg 
      cgagaggac gtcctgatg aaagttagt tcagggaaa 
      cgcttaacc gaacgaaga gttttcctc ttcgacggt 
      ttactcatc ttgtgtaaa ccgaactca agacgacag 
      agttccgtt caccaaaac cacgcagaa tgtcgctta 
      aaggagcgt tttttcatc cgaaaagtc gaaatcatc 
      gatcacttg gacaccgaa gagttgaag aacgcgttc 
      gaaatcgcg ccaagacac atgcaaccg tcggtgatt 
      ttgtgtgcg aaaacagcc gaagagaaa aactcgtgg 
      atggccgac ttggtgatg ttgaacacg cgctcgatg 
      ttggaacgc atccttgat agtattttg tcggatatt 
      gagcggaaa caccccctt aaacttcca ccgccgtct 
      ttgtacaaa tttgcggag cccgactcc aaggacaac 
      atcatcctc gagcagcgc gagaacgga ggtgtgccc 
      ctgatcaag ggcgcaaca ctgtataaa cttgttgaa 
      agactgact tatcatatt tacgccgat ccgaaattt 
      gtgcgcacg tttttgacg acgtatcgg agtttttgt 
      tcgccgaag gagttgctc gatttgctc attgaacga 
      tttcaaatt cccgatccg tcgctggtt tatgagcag 
      gagtgttgc gacacggat aaaatgcag aaaaataat 
      caaagggag gattggaaa aagtacagg aaggagtac 
      gtgcagccg gtgcagttc cgagtgttg aatgtgttg 
      aggcattgg gttgatcat catttttat gatttcgag 
      cgggatccc acacttttg gttaaattg agggatttt 
      ctggatgcg gtcaacggg aagagcatg aggaagtgg 
      gtggacagc gtgctcaag atattgcac agaaagatg 
      gaaaacgat aatcagcga cacatccgc ttcgccttc 
      gacgagcct ccgcccccg atcgaatgg cacatccct 
      tgcaccgaa gaagaatac ggaattcta accctccac 
      cctgtcgaa atcgcacgc caactaaca attctcgaa 
      ttcgatctg taccgaatg atcaaacct tccgaacta 
      gtcggagcc gtttggaca aaaaaagac aaagaaacg 
      acaagtccg aatttactc aaaatgatc aaacacaca 
      actaatgtg ggccgctgg ttggagaaa aacatcgtc 
      gaggcggaa aatttcgaa gaacgcgtc gcaatccta 
      aaccgaata atcgaaata atgatagtt ttatacgaa 
      ataaataat ttcaacgga gtcttgtcg atcgtctcg 
      acttgtgcc tcggcctcc gttcatcgc ctcaagctg 
      actttcgcc gcgctgacg caacagaac aagaaggcg 
      ctggaggag tgccgagcc gacgaccat ttcaaaaaa 
      taccaagag aaactacgc tccatcaac ccgccctgt 
      gtccccttc ctgggcatg tacctcacc aacatcctc 
      cacatcgag gagggcaat cccgacttc ctccccaac 
      acccaactc atcaacttc ttcaagcgc cgaaaagtc 
      gccgaaatc accggagaa atccaacaa taccagaac 
      caaccgtac tgtttcagc gtcgaatcg aaaatccgc 
      cacttcctc gaaaacctc aacccgttc ggcgacctc 
      tccgacacc gaaatcagc aactacctg tacaacaag 
      tcgatggag atcgagccc agaggccag aaacaagtc 
      cagaaattc ccccggaaa tggccacat ctgagcctc 
      aaaagtccg ggcattaaa tcaaaggcg aaaaccaca 
      gccacggcg gtcacaaat gcgattaat tccaccctg 
      aattcgtca aacatcaag agcgacgac tcaaagagc 
      gaagattcc tcgaaatcg gataacgag tttagcgtt 
      tttgcgccc gttcagttg ggcacctgc cagaacagc 
      cccacctta agcccggtc tcgccagcc acatccaac 
      accatcctg agctggttc cagcccaac cacaaccgc 
      agccccagt gtgggctcc atcatgtcc acgacgtcg 
      atgcgcaat ccgcccccg ccgccccca caagcgtca 
      aagtaccaa cactatagc caaagctcg acggggggc 
      tcgaacagc ccggggccg cacagcccc gcctcgccc 
      ggccccgtc caatcctac gaacctaac agtccgcgc 
      cgtccgcta accccgccc ccgcctccg cccccgctc 
      ccgccccgg cggcgccgc gacagcccc gaaatcagt 
      tccccgcaa ttaaagacc gatcctcat tggcgcgaa 
      tgctcccct cccccgctg cccccacgt cgcggcgat 
      cccggttcg tcccccagc tcccacatt caacactcg 
      catcattcc tttaactcg ttcgggggc gggaccatg 
      ccccgactc aaccccacc cacatgtcg cagttgcac 
      atgcggcgg cattcaacg gtccaaccg caggtgccg 
      aggaggacg gtccatgcg aacgtcacg cccttagtt 
      cctcagaac caaccagcg agtatttcg ccacggtat 
      gactcggcc acgcctcgg ctgccgccc aggccggcg 
      gcccggtcc gccgacatg gtcacggga cccatgttc 
      caatacccg agcactacc acaacttaa
    mRNA sequence
    • >TC015124 RA
      agataaccg atgtttaaa ctaaaatgg cggctgtca 
      actgatcgg tattttgtt cttcaatca ttcgaaagt 
      gaaaaattt ccaaaaagt cggtgttct ggtgtgttg 
      gagtggccg caaaatgtg caatataac gtgaacgtg 
      tccgttctg ggcccctct gtgcgtttc ccgtgctcc 
      agagtgaag gtttcgaac aatggcggt tataataca 
      gtgtgtgtt tgttttcga ttgatccag tgtgttgat 
      gtgtgcaat tgtcattga tttgtctaa tgatgaata 
      ctgtcaatg ccggcgaca acttttacg atttcacca 
      atgaggaaa attcggcca agtggaaag gagtgctca 
      ccaccgcct tgaagcggg tgctggagc aggtgcacc 
      cgtctttgg acgcccgcg aagatgccc tcgaatacg 
      tcgaaaacc tctgtttgc gccttctgg ccatgctct 
      gcgccaaac ctagtccac acacagtcc aggatgttg 
      aatatagag tacaaagca cgtttccta cccccatcg 
      ataagtggg ccttgaaag aggcccaag gcgccctcg 
      atcgaggca aaaagaagt ccgttttgc tgttgccag 
      ttgacaaaa ttcacagtt tattacaaa aggaattgc 
      tattgtaca aagtcgact cgaccgtct ccctctttc 
      tagtcgctg ttttggagt acatttcgg ccgatattt 
      taaaattgg cgggaaatt acgtgaaaa acatcaaac 
      atttcgaaa tcacctacc aggatgtcc agatttcga 
      tgaatgcgg ataaggcac tcatggata tgttctatc 
      aagatgaag gagggggac ctggtttac cgactgagt 
      cgcttttag tgtcgtcgg ctccgcgca cgagtctca 
      cgtatgagg aagtcgtaa gagagttaa tagcttcgg 
      aaaaagcat acatcaaag aactacaca tgttgatca 
      aagtgtttc gcgaggaaa ttatcaaac taagctccg 
      attcgaccg aaattgaac tgatttttt cgaacatta 
      tggatattt acgaactga cgtatactc tattgggta 
      gtttggagg acgtcatcg agatggcgc aagaccaaa 
      tgtcgtaca ttggcagct gtttcgaag agctggccg 
      aagccgccg aattcgacg tgtacataa aatacgcga 
      aagacgtaa ccgccccca catgtcgct ccaccctca 
      acagcctcc tctcgcgcc ccgacgtcg aaaacgcgc 
      tcatctcag ccggtcaag gcatgcgtc tcgccctca 
      agtattacc tgcctgcgc ttctaatgg gccccatcc 
      gacactgct tctcctaca tcgaataca tcaccattt 
      tacggggcc tgtccgtgg cccccgaag accgcgaaa 
      ccctcaccc aagtcgaag gcttactca aacccctcc 
      aaatcgagc tctcccggt gcgtcccct cccccgcca 
      tgctgcgca gctgctcca cccccttcc tccaagtgc 
      gggcgcgcc gacaagccg ccttggata aactgcacg 
      aaatcgaga agaacgtcg aaaattggg acccgaagg 
      atttgggac agtgttgca acgagttcg tgcgagagg 
      acgtcctga tgaaagtta gttcaggga aacgcttaa 
      ccgaacgaa gagttttcc tcttcgacg gtttactca 
      tcttgtgta aaccgaact caagacgac agagttccg 
      ttcaccaaa accacgcag aatgtcgct taaaggagc 
      gttttttca tccgaaaag tcgaaatca tcgatcact 
      tggacaccg aagagttga agaacgcgt tcgaaatcg 
      cgccaagac acatgcaac cgtcggtga ttttgtgtg 
      cgaaaacag ccgaagaga aaaactcgt ggatggccg 
      acttggtga tgttgaaca cgcgctcga tgttggaac 
      gcatccttg atagtattt tgtcggata ttgagcgga 
      aacaccccc ttaaacttc caccgccgt ctttgtaca 
      aatttgcgg agcccgact ccaaggaca acatcatcc 
      tcgagcagc gcgagaacg gaggtgtgc ccctgatca 
      agggcgcaa cactgtata aacttgttg aaagactga 
      cttatcata tttacgccg atccgaaat ttgtgcgca 
      cgtttttga cgacgtatc ggagttttt gttcgccga 
      aggagttgc tcgatttgc tcattgaac gatttcaaa 
      ttcccgatc cgtcgctgg tttatgagc aggagtgtt 
      gcgacacgg ataaaatgc agaaaaata atcaaaggg 
      aggattgga aaaagtaca ggaaggagt acgtgcagc 
      cggtgcagt tccgagtgt tgaatgtgt tgaggcatt 
      gggttgatc atcattttt atgatttcg agcgggatc 
      ccacacttt tggttaaat tgagggatt ttctggatg 
      cggtcaacg ggaagagca tgaggaagt gggtggaca 
      gcgtgctca agatattgc acagaaaga tggaaaacg 
      ataatcagc gacacatcc gcttcgcct tcgacgagc 
      ctccgcccc cgatcgaat ggcacatcc cttgcaccg 
      aagaagaat acggaattc taaccctcc accctgtcg 
      aaatcgcac gccaactaa caattctcg aattcgatc 
      tgtaccgaa tgatcaaac cttccgaac tagtcggag 
      ccgtttgga caaaaaaag acaaagaaa cgacaagtc 
      cgaatttac tcaaaatga tcaaacaca caactaatg 
      tgggccgct ggttggaga aaaacatcg tcgaggcgg 
      aaaatttcg aagaacgcg tcgcaatcc taaaccgaa 
      taatcgaaa taatgatag ttttatacg aaataaata 
      atttcaacg gagtcttgt cgatcgtct cgacttgtg 
      cctcggcct ccgttcatc gcctcaagc tgactttcg 
      ccgcgctga cgcaacaga acaagaagg cgctggagg 
      agtgccgag ccgacgacc atttcaaaa aataccaag 
      agaaactac gctccatca acccgccct gtgtcccct 
      tcctgggca tgtacctca ccaacatcc tccacatcg 
      aggagggca atcccgact tcctcccca acacccaac 
      tcatcaact tcttcaagc gccgaaaag tcgccgaaa 
      tcaccggag aaatccaac aataccaga accaaccgt 
      actgtttca gcgtcgaat cgaaaatcc gccacttcc 
      tcgaaaacc tcaacccgt tcggcgacc tctccgaca 
      ccgaaatca gcaactacc tgtacaaca agtcgatgg 
      agatcgagc ccagaggcc agaaacaag tccagaaat 
      tcccccgga aatggccac atctgagcc tcaaaagtc 
      cgggcatta aatcaaagg cgaaaacca cagccacgg 
      cggtcacaa atgcgatta attccaccc tgaattcgt 
      caaacatca agagcgacg actcaaaga gcgaagatt 
      cctcgaaat cggataacg agtttagcg tttttgcgc 
      ccgttcagt tgggcacct gccagaaca gccccacct 
      taagcccgg tctcgccag ccacatcca acaccatcc 
      tgagctggt tccagccca accacaacc gcagcccca 
      gtgtgggct ccatcatgt ccacgacgt cgatgcgca 
      atccgcccc cgccgcccc cacaagcgt caaagtacc 
      aacactata gccaaagct cgacggggg gctcgaaca 
      gcccggggc cgcacagcc ccgcctcgc ccggccccg 
      tccaatcct acgaaccta acagtccgc gccgtccgc 
      taaccccgc ccccgcctc cgcccccgc tcccgcccc 
      ggcggcgcc gcgacagcc ccgaaatca gttccccgc 
      aattaaaga ccgatcctc attggcgcg aatgctccc 
      ctcccccgc tgcccccac gtcgcggcg atcccggtt 
      cgtccccca gctcccaca ttcaacact cgcatcatt 
      cctttaact cgttcgggg gcgggacca tgccccgac 
      tcaacccca cccacatgt cgcagttgc acatgcggc 
      ggcattcaa cggtccaac cgcaggtgc cgaggagga 
      cggtccatg cgaacgtca cgcccttag ttcctcaga 
      accaaccag cgagtattt cgccacggt atgactcgg 
      ccacgcctc ggctgccgc ccaggccgg cggcccggt 
      ccgccgaca tggtcacgg gacccatgt tccaatacc 
      cgagcacta ccacaactt aatgaggga ggggcttcc 
      aaataagca agttttatc aaaatgatt tgaatttgt 
      tgtcgtgtg tacaaaaga cttgttcta tttattacg 
      tattttatg taagtatag agttggcga tgtttttcc 
      ccctcttac actgattga tgtacatta tgtaaatac 
      tgtaatata atgtaaatg gataaatat agttgccaa 
      aaagtgtaa tatggaaaa acagttttc tatgttgaa 
      tttttatga taatacatt gggatggtg cgattgact 
      cgcagactt aatcccgct atctcaacg tggaaagga 
      attgttaca gtatagatg tttatcttg agtttgcgt 
      aattgggga cttgcccca ccgccggac aagtcccca 
      atcgccccc ttaaattga tgatttttt aatctgtga 
      tagtagaaa atggaccat actttgtaa aaaaaa