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Gene information
General information
TC015114 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3085307..3090104
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015114
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02407

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015114 RA
      MAWTNYQIL LALVMVITG SINTLSTKW ADSLDSAGK 
      NGVVHVFDH PFFQASCMF IGEMLCLLT FKILYKVYS 
      RRSDGSEDV NELTRGNRN FSPFVLFLP AMCDMTATS 
      IMYIGLNLT YASSFQMFR GSVIIFVGL LSVGFLERI 
      LKKREWVGI FFVIVGLAV VGAADFLAK DTNAQNHGR 
      NDVITGDLL IVIAQIITA IQMVVEEKF VAGLDIPPL 
      QAIGWEGLF GFVVLGLLQ IPFYFIKAG PPFTNNPHG 
      TLEDAIDAL IQIGHSWQL MFAILGTII SIAFFNFAG 
      ISVTKEISA TTRMVLDSV RTIIIWVVS LMFLDQKFH 
      WLQLLGFVA LLYGMCLYN GITCTFIFI KIRAFFIRL 
      RYRGMEEDS IIENRTADL PDDSTPA
    CDS sequence
    • >TC015114 RA
      atggcttgg accaattat caaattctc ttggcgctt 
      gttatggtg ataacagga agtatcaac accttaagt 
      accaagtgg gctgacagt ctcgactcc gctggcaaa 
      aatggggtg gtgcacgtc ttcgaccac cccttcttc 
      caggcctct tgcatgttc atcggcgaa atgttgtgc 
      ttgctcact ttcaaaatc ctctacaag gtttacagc 
      cggcgctcg gacggctcc gaagacgtc aacgaactc 
      acacgcgga aatcgcaat ttcagccct tttgtgctc 
      ttcctgccc gccatgtgt gacatgacg gccacttcg 
      atcatgtac attggcctc aacctgacc tacgcctcc 
      agttttcaa atgtttagg ggctctgtg attattttc 
      gttggtttg ctcagtgtg gggttcctg gagcggatt 
      ctgaagaag cgggagtgg gtggggatt tttttcgtt 
      attgtgggg ttggcggtc gtgggggcg gccgacttt 
      ttggccaaa gacacaaat gcgcagaac catggccgc 
      aatgatgtg attacgggg gatcttttg attgtcatc 
      gcccagatt atcaccgct attcagatg gttgtggag 
      gagaagttt gtggctggg ctggacatt cccccgctt 
      caggcgatt ggttgggag ggacttttt gggtttgtg 
      gttttgggg ctgttgcaa ataccgttt tatttcatt 
      aaagcgggg cccccgttc actaataac ccccatggc 
      acgctagag gatgccatc gatgctctg atacagatt 
      gggcacagt tggcagcta atgtttgct attttagga 
      acaataatt tccatcgct ttcttcaac tttgctggc 
      ataagtgtg accaaagag atatcagct acgacccga 
      atggtccta gatagtgtc cgtacaata ataatatgg 
      gtagtaagt ttgatgttc cttgatcaa aaattccat 
      tggcttcag ttgttaggt ttcgtcgct ttattatac 
      ggaatgtgc ctttacaac ggaatcact tgcacattt 
      attttcata aaaattcga gcatttttc atccgactt 
      aggtaccga ggcatggag gaagacagc attattgaa 
      aatcgaacg gctgatcta ccagatgat tcaacaccg 
      gcctaa
    mRNA sequence
    • >TC015114 RA
      agtgacagc gtagtaatg ccacaaaaa tcagctgac 
      gtaaatatt ttgttttat cagtgactt tgtgtgatt 
      agcaacgaa aaaatggct tggaccaat tatcaaatt 
      ctcttggcg cttgttatg gtgataaca ggaagtatc 
      aacacctta agtaccaag tgggctgac agtctcgac 
      tccgctggc aaaaatggg gtggtgcac gtcttcgac 
      caccccttc ttccaggcc tcttgcatg ttcatcggc 
      gaaatgttg tgcttgctc actttcaaa atcctctac 
      aaggtttac agccggcgc tcggacggc tccgaagac 
      gtcaacgaa ctcacacgc ggaaatcgc aatttcagc 
      ccttttgtg ctcttcctg cccgccatg tgtgacatg 
      acggccact tcgatcatg tacattggc ctcaacctg 
      acctacgcc tccagtttt caaatgttt aggggctct 
      gtgattatt ttcgttggt ttgctcagt gtggggttc 
      ctggagcgg attctgaag aagcgggag tgggtgggg 
      atttttttc gttattgtg gggttggcg gtcgtgggg 
      gcggccgac tttttggcc aaagacaca aatgcgcag 
      aaccatggc cgcaatgat gtgattacg ggggatctt 
      ttgattgtc atcgcccag attatcacc gctattcag 
      atggttgtg gaggagaag tttgtggct gggctggac 
      attcccccg cttcaggcg attggttgg gagggactt 
      tttgggttt gtggttttg gggctgttg caaataccg 
      ttttatttc attaaagcg gggcccccg ttcactaat 
      aacccccat ggcacgcta gaggatgcc atcgatgct 
      ctgatacag attgggcac agttggcag ctaatgttt 
      gctatttta ggaacaata atttccatc gctttcttc 
      aactttgct ggcataagt gtgaccaaa gagatatca 
      gctacgacc cgaatggtc ctagatagt gtccgtaca 
      ataataata tgggtagta agtttgatg ttccttgat 
      caaaaattc cattggctt cagttgtta ggtttcgtc 
      gctttatta tacggaatg tgcctttac aacggaatc 
      acttgcaca tttattttc ataaaaatt cgagcattt 
      ttcatccga cttaggtac cgaggcatg gaggaagac 
      agcattatt gaaaatcga acggctgat ctaccagat 
      gattcaaca ccggcctaa agccacttg tttgaaacg 
      aaaagacta atcttattg tgaaactgt tttaaaaaa 
      tataaacga attgtttac ttaggtaaa atttatttc 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY