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Gene information
General information
TC015114 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 2129083..2133880
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015114
    iBeetle screen iB_02407
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015114 RA
      MAWTNYQIL LALVMVITG SINTLSTKW ADSLDSAGK 
NGVVHVFDH PFFQASCMF IGEMLCLLT FKILYKVYS 
RRSDGSEDV NELTRGNRN FSPFVLFLP AMCDMTATS 
IMYIGLNLT YASSFQMFR GSVIIFVGL LSVGFLERI 
LKKREWVGI FFVIVGLAV VGAADFLAK DTNAQNHGR 
NDVITGDLL IVIAQIITA IQMVVEEKF VAGLDIPPL 
QAIGWEGLF GFVVLGLLQ IPFYFIKAG PPFTNNPHG 
TLEDAIDAL IQIGHSWQL MFAILGTII SIAFFNFAG 
ISVTKEISA TTRMVLDSV RTIIIWVVS LMFLDQKFH 
WLQLLGFVA LLYGMCLYN GITCTFIFI KIRAFFIRL 
RYRGMEEDS IIENRTADL PDDSTPA
    CDS sequence
    • >TC015114 RA
      atggcttgg accaattat caaattctc ttggcgctt 
gttatggtg ataacagga agtatcaac accttaagt 
accaagtgg gctgacagt ctcgactcc gctggcaaa 
aatggggtg gtgcacgtc ttcgaccac cccttcttc 
caggcctct tgcatgttc atcggcgaa atgttgtgc 
ttgctcact ttcaaaatc ctctacaag gtttacagc 
cggcgctcg gacggctcc gaagacgtc aacgaactc 
acacgcgga aatcgcaat ttcagccct tttgtgctc 
ttcctgccc gccatgtgt gacatgacg gccacttcg 
atcatgtac attggcctc aacctgacc tacgcctcc 
agttttcaa atgtttagg ggctctgtg attattttc 
gttggtttg ctcagtgtg gggttcctg gagcggatt 
ctgaagaag cgggagtgg gtggggatt tttttcgtt 
attgtgggg ttggcggtc gtgggggcg gccgacttt 
ttggccaaa gacacaaat gcgcagaac catggccgc 
aatgatgtg attacgggg gatcttttg attgtcatc 
gcccagatt atcaccgct attcagatg gttgtggag 
gagaagttt gtggctggg ctggacatt cccccgctt 
caggcgatt ggttgggag ggacttttt gggtttgtg 
gttttgggg ctgttgcaa ataccgttt tatttcatt 
aaagcgggg cccccgttc actaataac ccccatggc 
acgctagag gatgccatc gatgctctg atacagatt 
gggcacagt tggcagcta atgtttgct attttagga 
acaataatt tccatcgct ttcttcaac tttgctggc 
ataagtgtg accaaagag atatcagct acgacccga 
atggtccta gatagtgtc cgtacaata ataatatgg 
gtagtaagt ttgatgttc cttgatcaa aaattccat 
tggcttcag ttgttaggt ttcgtcgct ttattatac 
ggaatgtgc ctttacaac ggaatcact tgcacattt 
attttcata aaaattcga gcatttttc atccgactt 
aggtaccga ggcatggag gaagacagc attattgaa 
aatcgaacg gctgatcta ccagatgat tcaacaccg 
gcctaa
    mRNA sequence
    • >TC015114 RA
      agtgacagc gtagtaatg ccacaaaaa tcagctgac 
gtaaatatt ttgttttat cagtgactt tgtgtgatt 
agcaacgaa aaaatggct tggaccaat tatcaaatt 
ctcttggcg cttgttatg gtgataaca ggaagtatc 
aacacctta agtaccaag tgggctgac agtctcgac 
tccgctggc aaaaatggg gtggtgcac gtcttcgac 
caccccttc ttccaggcc tcttgcatg ttcatcggc 
gaaatgttg tgcttgctc actttcaaa atcctctac 
aaggtttac agccggcgc tcggacggc tccgaagac 
gtcaacgaa ctcacacgc ggaaatcgc aatttcagc 
ccttttgtg ctcttcctg cccgccatg tgtgacatg 
acggccact tcgatcatg tacattggc ctcaacctg 
acctacgcc tccagtttt caaatgttt aggggctct 
gtgattatt ttcgttggt ttgctcagt gtggggttc 
ctggagcgg attctgaag aagcgggag tgggtgggg 
atttttttc gttattgtg gggttggcg gtcgtgggg 
gcggccgac tttttggcc aaagacaca aatgcgcag 
aaccatggc cgcaatgat gtgattacg ggggatctt 
ttgattgtc atcgcccag attatcacc gctattcag 
atggttgtg gaggagaag tttgtggct gggctggac 
attcccccg cttcaggcg attggttgg gagggactt 
tttgggttt gtggttttg gggctgttg caaataccg 
ttttatttc attaaagcg gggcccccg ttcactaat 
aacccccat ggcacgcta gaggatgcc atcgatgct 
ctgatacag attgggcac agttggcag ctaatgttt 
gctatttta ggaacaata atttccatc gctttcttc 
aactttgct ggcataagt gtgaccaaa gagatatca 
gctacgacc cgaatggtc ctagatagt gtccgtaca 
ataataata tgggtagta agtttgatg ttccttgat 
caaaaattc cattggctt cagttgtta ggtttcgtc 
gctttatta tacggaatg tgcctttac aacggaatc 
acttgcaca tttattttc ataaaaatt cgagcattt 
ttcatccga cttaggtac cgaggcatg gaggaagac 
agcattatt gaaaatcga acggctgat ctaccagat 
gattcaaca ccggcctaa agccacttg tttgaaacg 
aaaagacta atcttattg tgaaactgt tttaaaaaa 
tataaacga attgtttac ttaggtaaa atttatttc 
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