Status
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Gene information
General information
TC015110 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3124379..3131744
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015110
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02406

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015110 RA
      MRKTTRKPF YGSTNQPHY SHEFNDYDD TFGAGHSNK 
      YTRATTPKI FEKNWPPPF PSTDVNADY VFEDEEEGE 
      VTLEAENSI RNGPSRDGD CGKSDFRCS ASKCVPKTL 
      VCNGFKDCP DGTDEIDCL DYISKFSKK QNSKLAVLE 
      KERWDNVSV ATCALLCVQ SKEHHCRSF NYRKIDKTC 
      FLTNTNEGL TGALILYYP FDYYELTSE KINCSGMYI 
      CNNKKCIMR EKLCDGHDD CGDRMDEKG CKSEDFGYS 
      VRLTGSKNR NEGQIEVRA FGRTGYVCD DKFGLRDAN 
      VVCKELGFS LGAAEVKGN SFYAKNVTG KPFYMMDDV 
      SCVGNETKL MDCDFSGWG VHNCLEQES VGIVCKTPQ 
      EKCADDYWQ CDTGQECLP LAFVCDGLL DCTDGSDEG 
      SQHCDAPTE IRLVNGTNR QQGRVEIRH HGIWGTICD 
      DDFNEDAAK VICKHLGFK GSVVVKKDG YFGAGSGPI 
      WLDQVICSG NETSIENCT HWNWGEHNC EHSEDVGVI 
      CSNFYEHER HSMIPIPTI PQTYPDRCG YRKNNIFNR 
      NDDVHFRVV RGSVAQRGD YPWQAAIRV KGKSKAAHW 
      CGAVIISEK FALTAAHCL IGYSKGAYV VVAGDYNVD 
      EYEGTEQEA YIEDFYLHE NFRQGHKMN NDIALIKLK 
      GRGFRLNDD VQAICLPDS DTNYETDLN CTISGYGSI 
      ESGKSAFSH DLRAGWVPL QKREICTMP HVYGDALTE 
      GMICAGSLD EGIDSCDGD SGGPLACLY DGVFTLYGI 
      TSWGQHCGY ANKPGVYVK IAHYKRWID ENFHKHSLN
    CDS sequence
    • >TC015110 RA
      atgcgaaaa accacccgg aaaccattt tatggttcc 
      accaatcag ccacattac agccacgaa ttcaacgat 
      tacgatgat acttttggt gctggccac agtaataaa 
      tacacaaga gccacaaca ccgaaaatt ttcgaaaaa 
      aattggcct ccgcccttc ccctcaaca gacgtaaac 
      gccgactat gttttcgaa gacgaagaa gaaggagaa 
      gtgactcta gaagcggaa aattcaatc agaaatggg 
      ccttcacgt gatggtgat tgtggcaaa agtgatttt 
      cgttgttct gccagtaag tgtgttcca aaaactttg 
      gtctgtaac ggttttaaa gactgtcct gatggtacc 
      gacgaaatc gactgtctt gactacatc tcgaaattc 
      tccaagaag caaaacagc aaacttgca gttcttgag 
      aaagaaaga tgggataat gtcagtgtt gccacttgt 
      gccttgtta tgtgtccag tcaaaggaa catcactgc 
      cgatcgttc aattaccga aagattgac aaaacgtgt 
      tttttgacc aacactaat gaaggtcta acgggcgct 
      ttaatcctg tattatccc ttcgattat tacgaactg 
      accagcgaa aaaatcaac tgcagtggc atgtacatt 
      tgcaacaac aaaaaatgc ataatgagg gaaaaactg 
      tgcgatggt catgacgat tgtggcgac cgcatggac 
      gagaaagga tgcaagtct gaggatttt ggttacagt 
      gttcgcttg acgggatct aagaacaga aatgaaggg 
      caaattgaa gttagggcg tttggaagg acaggatac 
      gtgtgtgat gataaattt ggactgagg gatgctaat 
      gttgtgtgc aaggagttg ggtttttct ttaggcgcc 
      gctgaagtt aagggaaac tcgttctat gcgaaaaat 
      gtgacggga aaaccgttt tatatgatg gatgatgtt 
      agttgtgtg gggaacgag accaagtta atggattgt 
      gattttagt gggtgggga gttcacaac tgtttggaa 
      caggagagt gtgggaata gtttgcaaa actcctcaa 
      gaaaaatgt gccgacgac tattggcaa tgtgacacc 
      ggacaagaa tgtcttcct ctggctttt gtctgtgat 
      ggattactt gactgcact gatggttca gacgaagga 
      agtcagcac tgtgatgcc cctacggag attcgcctg 
      gtcaatggg acaaaccgc caacaaggc cgcgtggaa 
      atccgccac cacggcatc tggggcacc atctgcgac 
      gacgacttc aacgaggac gccgccaaa gtcatttgc 
      aaacatttg ggcttcaaa ggctcggtc gttgtgaaa 
      aaagacggc tatttcgga gccggctct ggaccgatt 
      tggttggat caagtgatt tgttcggga aatgagaca 
      agtattgaa aattgcact cattggaac tggggggag 
      cataactgc gaacacagc gaggatgtg ggggttatt 
      tgcagtaat ttttacgaa catgagagg cattcgatg 
      attccgata cccaccata ccacagacg tatcctgat 
      agatgtgga tatcgcaaa aacaacatt ttcaaccga 
      aacgacgac gtccacttc cgtgtagtt cgcggatcc 
      gtcgcccaa aggggtgac tacccctgg caggctgcc 
      atccgtgtc aagggcaaa agcaaagcc gcccattgg 
      tgcggtgct gtaattatt tccgaaaaa tttgcccta 
      acagcagcc cactgcctc atcggctac tccaaaggg 
      gcttacgtg gtggtcgca ggcgactat aacgtcgac 
      gagtacgaa gggaccgaa caagaagcc tacattgaa 
      gatttttat ctccacgaa aatttccgc caaggccat 
      aaaatgaac aatgacatt gcactgatc aagttgaaa 
      ggccgcgga ttcaggctg aacgatgac gtccaagcg 
      atttgttta ccagattcc gatacgaac tacgagacc 
      gatctgaac tgtaccata tctggttat gggtccatt 
      gaaagtgga aaatctgcc ttttcgcat gatttgaga 
      gctgggtgg gttccactg caaaaacgc gaaatttgt 
      accatgccc catgtgtat ggggacgct ttgaccgaa 
      gggatgatt tgcgctggg tccttggac gaagggatt 
      gattcgtgc gatggtgac agtggaggg cctttggct 
      tgtttatac gacggtgtt ttcactttg tatggaatc 
      acgtcctgg ggacaacac tgtggatac gctaataaa 
      cctggagtc tacgtcaaa atagcgcat tacaagagg 
      tggattgac gagaatttc cataagcat tcgctaaat 
      taa
    mRNA sequence
    • >TC015110 RA
      nnnnccaga aacatacaa catcaacgg gaaccaaaa 
      ctcctaccg tggaaccat tgactcagg aacctttgg 
      atcaaacag agacccagg ttaccaaag accagacac 
      ttccaacac caagaggaa ccaaaactc caatcgtgg 
      aaccacata tatcgtacc aacagcttc caaccgaga 
      aacgtggta ccaccaaaa cccactaga tgtggaaac 
      caacactcc gaattacga ccaagtggt taacatcaa 
      ccaaatgcg aaaaaccac ccggaaacc attttatgg 
      ttccaccaa tcagccaca ttacagcca cgaattcaa 
      cgattacga tgatacttt tggtgctgg ccacagtaa 
      taaatacac aagagccac aacaccgaa aattttcga 
      aaaaaattg gcctccgcc cttcccctc aacagacgt 
      aaacgccga ctatgtttt cgaagacga agaagaagg 
      agaagtgac tctagaagc ggaaaattc aatcagaaa 
      tgggccttc acgtgatgg tgattgtgg caaaagtga 
      ttttcgttg ttctgccag taagtgtgt tccaaaaac 
      tttggtctg taacggttt taaagactg tcctgatgg 
      taccgacga aatcgactg tcttgacta catctcgaa 
      attctccaa gaagcaaaa cagcaaact tgcagttct 
      tgagaaaga aagatggga taatgtcag tgttgccac 
      ttgtgcctt gttatgtgt ccagtcaaa ggaacatca 
      ctgccgatc gttcaatta ccgaaagat tgacaaaac 
      gtgtttttt gaccaacac taatgaagg tctaacggg 
      cgctttaat cctgtatta tcccttcga ttattacga 
      actgaccag cgaaaaaat caactgcag tggcatgta 
      catttgcaa caacaaaaa atgcataat gagggaaaa 
      actgtgcga tggtcatga cgattgtgg cgaccgcat 
      ggacgagaa aggatgcaa gtctgagga ttttggtta 
      cagtgttcg cttgacggg atctaagaa cagaaatga 
      agggcaaat tgaagttag ggcgtttgg aaggacagg 
      atacgtgtg tgatgataa atttggact gagggatgc 
      taatgttgt gtgcaagga gttgggttt ttctttagg 
      cgccgctga agttaaggg aaactcgtt ctatgcgaa 
      aaatgtgac gggaaaacc gttttatat gatggatga 
      tgttagttg tgtggggaa cgagaccaa gttaatgga 
      ttgtgattt tagtgggtg gggagttca caactgttt 
      ggaacagga gagtgtggg aatagtttg caaaactcc 
      tcaagaaaa atgtgccga cgactattg gcaatgtga 
      caccggaca agaatgtct tcctctggc ttttgtctg 
      tgatggatt acttgactg cactgatgg ttcagacga 
      aggaagtca gcactgtga tgcccctac ggagattcg 
      cctggtcaa tgggacaaa ccgccaaca aggccgcgt 
      ggaaatccg ccaccacgg catctgggg caccatctg 
      cgacgacga cttcaacga ggacgccgc caaagtcat 
      ttgcaaaca tttgggctt caaaggctc ggtcgttgt 
      gaaaaaaga cggctattt cggagccgg ctctggacc 
      gatttggtt ggatcaagt gatttgttc gggaaatga 
      gacaagtat tgaaaattg cactcattg gaactgggg 
      ggagcataa ctgcgaaca cagcgagga tgtgggggt 
      tatttgcag taattttta cgaacatga gaggcattc 
      gatgattcc gatacccac cataccaca gacgtatcc 
      tgatagatg tggatatcg caaaaacaa cattttcaa 
      ccgaaacga cgacgtcca cttccgtgt agttcgcgg 
      atccgtcgc ccaaagggg tgactaccc ctggcaggc 
      tgccatccg tgtcaaggg caaaagcaa agccgccca 
      ttggtgcgg tgctgtaat tatttccga aaaatttgc 
      cctaacagc agcccactg cctcatcgg ctactccaa 
      aggggctta cgtggtggt cgcaggcga ctataacgt 
      cgacgagta cgaagggac cgaacaaga agcctacat 
      tgaagattt ttatctcca cgaaaattt ccgccaagg 
      ccataaaat gaacaatga cattgcact gatcaagtt 
      gaaaggccg cggattcag gctgaacga tgacgtcca 
      agcgatttg tttaccaga ttccgatac gaactacga 
      gaccgatct gaactgtac catatctgg ttatgggtc 
      cattgaaag tggaaaatc tgccttttc gcatgattt 
      gagagctgg gtgggttcc actgcaaaa acgcgaaat 
      ttgtaccat gccccatgt gtatgggga cgctttgac 
      cgaagggat gatttgcgc tgggtcctt ggacgaagg 
      gattgattc gtgcgatgg tgacagtgg agggccttt 
      ggcttgttt atacgacgg tgttttcac tttgtatgg 
      aatcacgtc ctggggaca acactgtgg atacgctaa 
      taaacctgg agtctacgt caaaatagc gcattacaa 
      gaggtggat tgacgagaa tttccataa gcattcgct 
      aaattaact tagttatgt agtttaagt aggtgataa 
      gaataatta ttgtaacaa ttggttatg taaataaat 
      gaaaattcg gacaagtct gctaaattt ccaacccct 
      gttcaagtt caataaatt attgaaatg acc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY