Status
Please wait ...
Gene information
General information
TC015102 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3313397..3315111
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015102
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02405

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head remaining - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax partiallyremaining - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015102 RA
      MLLTNSICG DLVTIFLTL VTVLTLYFK WCHQYWEKL 
      NLPSLPPSI PFGTSLGPK GHFGLRMKH IYDKMRQKG 
      WKHGGIYLM QRPFYVVVD LDYVKNIMT KDFHHFSDR 
      GIFHNEKAD PLSAHLFNI SGPKWRNLR SKLTPTFTS 
      GKMKLMFQT LLDCEVNLR KQVEQHYEA KKPIDIKEV 
      LGCFTTDII GSCAFGLDC NSFKEENSP FRVYGRRVF 
      DRSGFIRTL KRTFASNFP NLAKLVNIA LVPRDISDF 
      FINIVKDTV EYREKNKIV RNDFLQLLL DLKTEESGN 
      DGTALTFEE IAAQSFVFF IAGFETSST TMAFALYEL 
      ARHQDIQDQ VRDEIESVL KKYDGKITY EAIQEMSFL 
      GQVIDEALR MYPPGPLVP RRCVKDYKV PNTDVVIEK 
      GVMVLIPIL GIHYDEKYY PDPEKFDPE RFSEENKAL 
      RHSYAHIPF GEGPRICIG LRFGLMQSK VGLVSLLRN 
      FKFTVNEKT QEPLKMKTN SLVLAVEGE IWLNVHRL
    CDS sequence
    • >TC015102 RA
      atgctactc acaaactca atttgtggt gatttagtc 
      acaattttt ctcacttta gttacagtt ctcacactt 
      tatttcaaa tggtgccac cagtattgg gaaaaactc 
      aacctgccc tccctgcct ccttcaatc cctttcggg 
      accagctta ggccctaag ggacacttc ggcctcaga 
      atgaagcac atttacgat aaaatgagg caaaagggg 
      tggaaacac gggggcata tatctcatg caacggcct 
      ttctacgta gttgtagac ttagactac gtgaaaaac 
      atcatgacg aaagatttc caccacttt tccgaccgg 
      ggcattttc cacaacgaa aaagccgac cctctcagc 
      gcccacttg ttcaacata agtggcccc aaatggaga 
      aacttgcgg agcaaatta accccaact ttcacatcc 
      ggcaaaatg aagctgatg tttcaaact ctcctggac 
      tgtgaagtc aatttgcgg aaacaggtg gaacaacat 
      tacgaggcg aaaaaacca attgatatt aaagaagtg 
      ttagggtgt ttcaccacc gatataatc ggctcttgt 
      gccttcgga cttgactgt aactcgttt aaagaggag 
      aattcaccg tttcgggtt tacggacgt agagttttc 
      gaccgtagc ggttttatc cgcactttg aaacgcacg 
      tttgctagc aatttcccc aatttggcg aaacttgtg 
      aacattgcc ttggtcccg agggacatc tccgatttt 
      tttatcaac attgtgaag gacacggtt gagtatagg 
      gagaaaaat aagatcgtc aggaacgat tttttgcaa 
      ctgcttctg gacctgaag acagaggag tcgggaaat 
      gacggcacg gccttaaca tttgaagaa attgcagcc 
      caaagtttt gttttcttc atcgctggt tttgaaacg 
      tcttccact accatggcc ttcgctctg tacgaactg 
      gccagacac caagacatt caggatcaa gtcagggat 
      gaaattgag tcagttttg aaaaagtac gacgggaaa 
      atcacgtat gaagcaatc caagaaatg agctttttg 
      gggcaagtg attgacgag gcgcttagg atgtacccc 
      ccggggccc ctcgtgccc aggcgatgc gtcaaagac 
      tacaaagtg cccaacact gatgttgtt attgaaaaa 
      ggtgtaatg gttttaatc ccaatcctt gggatacat 
      tacgacgaa aagtattat cctgatcct gagaagttc 
      gatcctgag aggtttagt gaagagaat aaggcctta 
      aggcacagt tatgcccat attccgttt ggtgaaggg 
      cctagaatt tgtatagga ttacgcttt ggactaatg 
      cagtctaaa gtgggtcta gtctctttg ttgaggaat 
      ttcaaattt acggtcaat gagaagacg caagaacct 
      ttgaagatg aaaaccaat tctttggtt ttggccgtt 
      gagggcgaa atttggctc aatgttcat agattgtaa
    mRNA sequence
    • >TC015102 RA
      aatgacgtg ttacatttt aataataaa tacaacagt 
      gattgctct cggaagtag tcaattcaa gtgctgtaa 
      ccatgctac tcacaaact caatttgtg gtgatttag 
      tcacaattt ttctcactt tagttacag ttctcacac 
      tttatttca aatggtgcc accagtatt gggaaaaac 
      tcaacctgc cctccctgc ctccttcaa tccctttcg 
      ggaccagct taggcccta agggacact tcggcctca 
      gaatgaagc acatttacg ataaaatga ggcaaaagg 
      ggtggaaac acgggggca tatatctca tgcaacggc 
      ctttctacg tagttgtag acttagact acgtgaaaa 
      acatcatga cgaaagatt tccaccact tttccgacc 
      ggggcattt tccacaacg aaaaagccg accctctca 
      gcgcccact tgttcaaca taagtggcc ccaaatgga 
      gaaacttgc ggagcaaat taaccccaa ctttcacat 
      ccggcaaaa tgaagctga tgtttcaaa ctctcctgg 
      actgtgaag tcaatttgc ggaaacagg tggaacaac 
      attacgagg cgaaaaaac caattgata ttaaagaag 
      tgttagggt gtttcacca ccgatataa tcggctctt 
      gtgccttcg gacttgact gtaactcgt ttaaagagg 
      agaattcac cgtttcggg tttacggac gtagagttt 
      tcgaccgta gcggtttta tccgcactt tgaaacgca 
      cgtttgcta gcaatttcc ccaatttgg cgaaacttg 
      tgaacattg ccttggtcc cgagggaca tctccgatt 
      tttttatca acattgtga aggacacgg ttgagtata 
      gggagaaaa ataagatcg tcaggaacg attttttgc 
      aactgcttc tggacctga agacagagg agtcgggaa 
      atgacggca cggccttaa catttgaag aaattgcag 
      cccaaagtt ttgttttct tcatcgctg gttttgaaa 
      cgtcttcca ctaccatgg ccttcgctc tgtacgaac 
      tggccagac accaagaca ttcaggatc aagtcaggg 
      atgaaattg agtcagttt tgaaaaagt acgacggga 
      aaatcacgt atgaagcaa tccaagaaa tgagctttt 
      tggggcaag tgattgacg aggcgctta ggatgtacc 
      ccccggggc ccctcgtgc ccaggcgat gcgtcaaag 
      actacaaag tgcccaaca ctgatgttg ttattgaaa 
      aaggtgtaa tggttttaa tcccaatcc ttgggatac 
      attacgacg aaaagtatt atcctgatc ctgagaagt 
      tcgatcctg agaggttta gtgaagaga ataaggcct 
      taaggcaca gttatgccc atattccgt ttggtgaag 
      ggcctagaa tttgtatag gattacgct ttggactaa 
      tgcagtcta aagtgggtc tagtctctt tgttgagga 
      atttcaaat ttacggtca atgagaaga cgcaagaac 
      ctttgaaga tgaaaacca attctttgg ttttggccg 
      ttgagggcg aaatttggc tcaatgttc atagattgt 
      aaaagtata caaaataat aaatattaa aagttgtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY