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Gene information
General information
TC015099 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 2384484..2386390
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015099
    iBeetle screen iB_02403
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015099 RA
      MWFGSILTY FSLAILVES KSVPTISPI SREIKVING 
      NDAQIGQFP WQVFIICLR SNLLVYCNG AIINEKWVL 
      TSASCIDIV GMTAYVYPG IVNLNSSTP ELRISREVL 
      IHEDFDFAT LQNDIALIK LPTDLEFNE NIKPIALDE 
      DGLPPGTSA TISGWGHSS HDDVQNFNL TSVLQYAEV 
      PVIDNKECE QIYGPDVVT SSVVCTKNP NSVKGPCLH 
      DGGAPLVII NAETKNPIH VGIFSFVGD SACEKNYPA 
      GYTRTAAFR NWIKEKTGV
    CDS sequence
    • >TC015099 RA
      atgtggttc ggcagcatt ttgacatat ttctctctc 
      gcaattttg gtagagtct aaaagtgtg ccaacaatt 
      tcacccata agtcgagaa attaaagtt attaacgga 
      aacgatgcc caaattggt caattcccg tggcaagta 
      tttatcatt tgtttacgc tcaaatctc cttgtctac 
      tgtaacggt gccataatt aatgaaaaa tgggttctg 
      acatctgca tcttgcatt gatatagtt ggcatgact 
      gcatatgtg tatccagga atagttaat cttaattca 
      tcgacaccg gaacttcgt attagtagg gaagtgctg 
      attcatgaa gattttgat tttgcgact ctacagaat 
      gacattgct ttaataaaa cttccaact gatttggaa 
      tttaacgaa aacattaaa ccaattgct ttagatgaa 
      gatgggtta ccacctgga acaagtgcc actatcagt 
      ggctgggga cattccagt cacgacgac gttcagaac 
      ttcaatcta acctctgtt ctgcaatat gctgaagtc 
      cctgtgatc gataacaaa gagtgtgaa caaatttac 
      ggtcctgac gttgttact tcaagtgtg gtttgcacg 
      aaaaaccca aattcggtt aaaggtccc tgtttacac 
      gacggtgga gctccttta gttattatc aatgctgag 
      acgaaaaat ccaatacat gttgggatt ttcagtttt 
      gtaggggat agcgcttgc gagaaaaat tatcctgcg 
      ggatacacc aggactgca gcgttcaga aattggata 
      aaggaaaaa actggtgtt tga
    mRNA sequence
    • >TC015099 RA
      gaatagata aatattttt taactttat tatctctaa 
      ctagctaaa tttcagtaa taaagctaa tacgaactg 
      tgaaaaatg tggttcggc agcattttg acatatttc 
      tctctcgca attttggta gagtctaaa agtgtgcca 
      acaatttca cccataagt cgagaaatt aaagttatt 
      aacggaaac gatgcccaa attggtcaa ttcccgtgg 
      caagtattt atcatttgt ttacgctca aatctcctt 
      gtctactgt aacggtgcc ataattaat gaaaaatgg 
      gttctgaca tctgcatct tgcattgat atagttggc 
      atgactgca tatgtgtat ccaggaata gttaatctt 
      aattcatcg acaccggaa cttcgtatt agtagggaa 
      gtgctgatt catgaagat tttgatttt gcgactcta 
      cagaatgac attgcttta ataaaactt ccaactgat 
      ttggaattt aacgaaaac attaaacca attgcttta 
      gatgaagat gggttacca cctggaaca agtgccact 
      atcagtggc tggggacat tccagtcac gacgacgtt 
      cagaacttc aatctaacc tctgttctg caatatgct 
      gaagtccct gtgatcgat aacaaagag tgtgaacaa 
      atttacggt cctgacgtt gttacttca agtgtggtt 
      tgcacgaaa aacccaaat tcggttaaa ggtccctgt 
      ttacacgac ggtggagct cctttagtt attatcaat 
      gctgagacg aaaaatcca atacatgtt gggattttc 
      agttttgta ggggatagc gcttgcgag aaaaattat 
      cctgcggga tacaccagg actgcagcg ttcagaaat 
      tggataaag gaaaaaact ggtgtttga aaagtatta 
      tatttattg tacaaaaaa aatacctac tttgctatt 
      tctattttt ttttaagtt ctgtacaga tagattttt 
      tatttgttt aattttaat taaaaaaac tggtgatta