Status
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Gene information
General information
TC015096 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3356730..3372514
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015096
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02402

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015096 RA
      MSAKAIREA TGKNLINKH LPGGTAAVQ CRFASVEPE 
      TKFSELVAT HPWLKSEKL VVKPDQLIK RRGKLGLIA 
      VNKDLAEVQ QWISDRMNK EQKIGAATG KLRNFIIEP 
      FVPHKDNEE VYVCIYSHR HGDTILFYH QGGVDIGDV 
      DSKAVKLEV PVGESVDEK RITEALLSQ VDASKKAMI 
      ANFVNNLYK LYVDLYFTY LEINPLVVT QKEIYVLDL 
      AAKLDATAD FICKPMWGE IDYPPPFGR DAYPEEAYI 
      ADLDSKSGA SLKLTILNK SGRIWTMVA GGGASVIYS 
      DTICDLGGA KELANYGEY SGAPSEQQT YEYAKTILS 
      LMTQEKHPQ GKVLITGGG IANFTNVAA TFRGIITAL 
      VEFQQRLIE HKVSIFVRR AGPNYQEGL RRMREVGQT 
      LGIPLYVFG PETHMTAIC GMALGTRPI PQENNVEFA 
      TANFLLPSG EKEPNKKSL GVEGVDGGV AAAAACSRK 
      IQGDSLGVQ TPMFTNKTK SIVWGMQTR AVQSMLDFD 
      FVCSRPEPS VVAIIYPFT GDHKQKFYW GHKEILIPV 
      YKKMNDAMT KHPEADVLV NFASLRSAY QSTVETMEF 
      PQIRTIAII AEGIPENQT RKLIKLANE KKVAIIGPA 
      TVGGVKPGC FKIGNTGGM MDNILHSKL YRSGSVAYV 
      SRSGGMSNE LNNIVSKAT DGVYEGVAI GGDRYPGTT 
      FMDHIMRYQ GDENVKMII LLGEVGGVE EYEVCNALK 
      NKIITKPLV AWCIGTCAG MFTSEVQFG HAGSCANSN 
      QETATAKNE ALKAAGAHV PASFDYLGE VIQEVYEKL 
      VIQGVIIPR AEVPPPTVP MDYSWAREL GLIRKPASF 
      MTSICDERG QELIYAGMP ISDVLQKNV GIGGVISLL 
      WFQRCLPTY VCKFFEMCL MVTADHGPA VSGAHNTIV 
      CARAGKDLV SSLVSGLLT IGDRFGGAL DGAAKQFSE 
      AYDTGMHPA EFVNHMRNK GQLIMGIGH RVKSINNPD 
      QRVKIVKEF VMANFPATP LLLYALEVE KITTSKKPN 
      LILNVDGVI ACSFVDMLR NCGSFTSEE AQEYINIGA 
      INSLFVLGR SIGFIGHFM DQKRLKQGL YRHPWDDIS 
      YVLPEQYNN
    CDS sequence
    • >TC015096 RA
      atgtcggcg aaggcaata agagaagcg accggaaaa 
      aatctaatc aacaaacac ttacctggc ggtaccgcc 
      gctgtgcaa tgtcgcttc gcttcggtc gaacccgaa 
      acgaaattt tccgaatta gtggccaca catccatgg 
      ttgaaaagc gaaaagttg gttgtgaaa cctgaccaa 
      cttatcaaa cggagagga aaattaggt ttgatcgct 
      gtcaataaa gatttagcc gaagtgcag caatggata 
      agcgatcgt atgaacaaa gagcaaaaa atcggtgcc 
      gccaccggc aaacttcgc aattttatc atcgaacct 
      tttgttcct cacaaagac aacgaagaa gtctatgtt 
      tgtatttat tcgcaccga catggcgat acgatcctg 
      ttttatcat caaggtggc gttgatatc ggtgatgtt 
      gattcgaaa gctgtgaaa ttggaggtt cctgtgggt 
      gaaagtgtc gatgaaaag cgcatcacg gaggctttg 
      ttgagtcaa gttgatgcg agtaaaaag gcgatgatt 
      gcgaatttt gtgaataat ttgtacaag ttgtacgtt 
      gatttgtat tttacttat ttggaaatt aatccattg 
      gtcgtcacc cagaaggag atttatgtt ttggatttg 
      gcggctaag ttggacgct acggctgat tttatttgt 
      aagccaatg tggggcgag attgattat ccgccgccg 
      tttggacgt gatgcatat ccagaggag gcctacatt 
      gccgacctt gattcaaaa tcgggcgcc agtctcaag 
      ctcacaatt ttgaataaa tcgggacgg atttggaca 
      atggtggct gggggcggc gcctccgtg atttacagc 
      gacacgatt tgcgatcta gggggcgcc aaagagctg 
      gcaaattat ggcgaatat tcgggggcg ccttccgaa 
      caacaaacc tacgaatat gccaagacg attctctcg 
      cttatgacg caagagaag cacccccag ggtaaagtt 
      ttgataact gggggtggg attgcgaat tttacaaat 
      gtggcggcg acttttcgc ggtattatc accgctttg 
      gtggagttc caacaacgt ttgatcgag cacaaagtc 
      tcgattttc gtccggaga gctggaccg aattatcaa 
      gagggcttg cggcggatg agggaggtt ggccagact 
      ttggggatt cccctgtac gtgttcggg cctgagaca 
      cacatgacg gccatttgt ggcatggcg ctgggcacg 
      cgccccatt ccacaggag aataacgtt gaatttgca 
      acggctaat tttttgctg cccagtggt gagaaagag 
      ccgaataag aagagtttg ggggtggaa ggggtggat 
      gggggggtg gcggctgcc gccgcttgc agtaggaaa 
      atacaaggc gactcattg ggcgtccaa acacccatg 
      tttaccaac aaaacgaaa tcaatcgtt tggggaatg 
      caaacacga gccgtgcaa agcatgctt gatttcgac 
      ttcgtgtgc agtcggccg gaaccgagc gttgtcgcc 
      attatctat ccatttacc ggggatcac aagcagaaa 
      ttctattgg ggacataag gaaattcta attccagtt 
      tataaaaaa atgaacgac gctatgacg aagcatccc 
      gaagccgac gtccttgtc aacttcgcg tcgcttcgt 
      tccgcttat caaagcacc gtcgaaacg atggaattc 
      ccccaaatc cgaacaatc gcgatcatt gccgaaggc 
      atccccgaa aatcaaacc cgcaaactg atcaaacta 
      gccaatgag aagaaggtt gcaattatt ggacctgcc 
      acagtgggc ggagtcaag ccagggtgt ttcaaaatt 
      ggcaacact ggcggaatg atggataat attctacat 
      tcgaaattg taccgctct ggaagtgtg gcttatgtt 
      tcaaggtca ggtgggatg tccaatgag cttaataat 
      attgtatcg aaagccacc gatggtgtg tatgagggc 
      gtggccata ggcggggat aggtatccc ggtaccacc 
      ttcatggac catattatg cgttaccaa ggcgatgag 
      aacgtcaaa atgattatt ttattgggc gaagtgggc 
      ggagttgaa gagtatgaa gtctgcaac gcccttaag 
      aataaaatt attaccaaa ccgttagtg gcctggtgt 
      attgggacc tgtgcgggc atgttcacc tccgaagtc 
      caattcggc cacgccggc tcttgcgcc aactcgaac 
      caagaaaca gcaacggcc aaaaacgag gccttaaag 
      gctgccggt gcccacgtc ccagcctcc tttgattac 
      ctcggcgag gtgattcaa gaagtttac gaaaagctg 
      gtaatacag ggcgtgata atcccacgt gcggaagtg 
      ccgccacct accgtcccg atggactac tcgtgggca 
      cgtgaactg ggcctaatt cgcaaaccg gccagtttt 
      atgacgtca atttgtgac gaaagaggc caagaattg 
      atctatgca ggcatgcca atctccgac gttttacaa 
      aaaaatgta ggcattggt ggtgtgatt tccctgttg 
      tggttccag cgttgttta ccaacttat gtttgcaaa 
      tttttcgaa atgtgtctg atggtgacg gccgatcat 
      gggcctgcg gtctctggg gcccataac acgattgtg 
      tgtgcacgt gctggcaaa gatctggtc tcgagtctt 
      gtgtctggg ctgttgacc attggggac cggtttggg 
      ggcgcgcta gatggcgct gccaagcag ttcagtgag 
      gcgtatgac acgggcatg cacccggcc gagtttgtt 
      aaccatatg aggaataaa ggacagttg attatgggg 
      atagggcac agggtcaag tctattaat aatccagat 
      caaagggtg aaaattgtc aaggagttc gtgatggcg 
      aatttcccg gcgacgcca ctgctgttg tacgccttg 
      gaagtggag aagatcaca acgagcaaa aaaccgaac 
      ttgattctg aacgttgat ggggtgatt gcatgctcg 
      tttgtggac atgctgcgg aattgtggc agttttacc 
      agtgaggag gctcaagag tacataaat attggcgca 
      attaactcc cttttcgtg ctggggcgc tcgataggg 
      ttcattggc cacttcatg gaccagaag aggctgaag 
      caagggttg tacagacac ccatgggat gatatttcc 
      tacgtactt ccagagcaa tataacaac taa
    mRNA sequence
    • >TC015096 RA
      acgcgtttt tgaccagtc aaaactctg aagtgacac 
      aagtttgct gtttgtagt ttcaactcg aaaattggg 
      gtaattaac ccactttta gtaattctg tgactgaaa 
      tttgaccca attcttgga aaattccgt gtttttggc 
      atcgaatta aactagaaa aatgtcggc gaaggcaat 
      aagagaagc gaccggaaa aaatctaat caacaaaca 
      cttacctgg cggtaccgc cgctgtgca atgtcgctt 
      cgcttcggt cgaacccga aacgaaatt ttccgaatt 
      agtggccac acatccatg gttgaaaag cgaaaagtt 
      ggttgtgaa acctgacca acttatcaa acggagagg 
      aaaattagg tttgatcgc tgtcaataa agatttagc 
      cgaagtgca gcaatggat aagcgatcg tatgaacaa 
      agagcaaaa aatcggtgc cgccaccgg caaacttcg 
      caattttat catcgaacc ttttgttcc tcacaaaga 
      caacgaaga agtctatgt ttgtattta ttcgcaccg 
      acatggcga tacgatcct gttttatca tcaaggtgg 
      cgttgatat cggtgatgt tgattcgaa agctgtgaa 
      attggaggt tcctgtggg tgaaagtgt cgatgaaaa 
      gcgcatcac ggaggcttt gttgagtca agttgatgc 
      gagtaaaaa ggcgatgat tgcgaattt tgtgaataa 
      tttgtacaa gttgtacgt tgatttgta ttttactta 
      tttggaaat taatccatt ggtcgtcac ccagaagga 
      gatttatgt tttggattt ggcggctaa gttggacgc 
      tacggctga ttttatttg taagccaat gtggggcga 
      gattgatta tccgccgcc gtttggacg tgatgcata 
      tccagagga ggcctacat tgccgacct tgattcaaa 
      atcgggcgc cagtctcaa gctcacaat tttgaataa 
      atcgggacg gatttggac aatggtggc tgggggcgg 
      cgcctccgt gatttacag cgacacgat ttgcgatct 
      agggggcgc caaagagct ggcaaatta tggcgaata 
      ttcgggggc gccttccga acaacaaac ctacgaata 
      tgccaagac gattctctc gcttatgac gcaagagaa 
      gcaccccca gggtaaagt tttgataac tgggggtgg 
      gattgcgaa ttttacaaa tgtggcggc gacttttcg 
      cggtattat caccgcttt ggtggagtt ccaacaacg 
      tttgatcga gcacaaagt ctcgatttt cgtccggag 
      agctggacc gaattatca agagggctt gcggcggat 
      gagggaggt tggccagac tttggggat tcccctgta 
      cgtgttcgg gcctgagac acacatgac ggccatttg 
      tggcatggc gctgggcac gcgccccat tccacagga 
      gaataacgt tgaatttgc aacggctaa ttttttgct 
      gcccagtgg tgagaaaga gccgaataa gaagagttt 
      gggggtgga aggggtgga tgggggggt ggcggctgc 
      cgccgcttg cagtaggaa aatacaagg cgactcatt 
      gggcgtcca aacacccat gtttaccaa caaaacgaa 
      atcaatcgt ttggggaat gcaaacacg agccgtgca 
      aagcatgct tgatttcga cttcgtgtg cagtcggcc 
      ggaaccgag cgttgtcgc cattatcta tccatttac 
      cggggatca caagcagaa attctattg gggacataa 
      ggaaattct aattccagt ttataaaaa aatgaacga 
      cgctatgac gaagcatcc cgaagccga cgtccttgt 
      caacttcgc gtcgcttcg ttccgctta tcaaagcac 
      cgtcgaaac gatggaatt cccccaaat ccgaacaat 
      cgcgatcat tgccgaagg catccccga aaatcaaac 
      ccgcaaact gatcaaact agccaatga gaagaaggt 
      tgcaattat tggacctgc cacagtggg cggagtcaa 
      gccagggtg tttcaaaat tggcaacac tggcggaat 
      gatggataa tattctaca ttcgaaatt gtaccgctc 
      tggaagtgt ggcttatgt ttcaaggtc aggtgggat 
      gtccaatga gcttaataa tattgtatc gaaagccac 
      cgatggtgt gtatgaggg cgtggccat aggcgggga 
      taggtatcc cggtaccac cttcatgga ccatattat 
      gcgttacca aggcgatga gaacgtcaa aatgattat 
      tttattggg cgaagtggg cggagttga agagtatga 
      agtctgcaa cgcccttaa gaataaaat tattaccaa 
      accgttagt ggcctggtg tattgggac ctgtgcggg 
      catgttcac ctccgaagt ccaattcgg ccacgccgg 
      ctcttgcgc caactcgaa ccaagaaac agcaacggc 
      caaaaacga ggccttaaa ggctgccgg tgcccacgt 
      cccagcctc ctttgatta cctcggcga ggtgattca 
      agaagttta cgaaaagct ggtaataca gggcgtgat 
      aatcccacg tgcggaagt gccgccacc taccgtccc 
      gatggacta ctcgtgggc acgtgaact gggcctaat 
      tcgcaaacc ggccagttt tatgacgtc aatttgtga 
      cgaaagagg ccaagaatt gatctatgc aggcatgcc 
      aatctccga cgttttaca aaaaaatgt aggcattgg 
      tggtgtgat ttccctgtt gtggttcca gcgttgttt 
      accaactta tgtttgcaa atttttcga aatgtgtct 
      gatggtgac ggccgatca tgggcctgc ggtctctgg 
      ggcccataa cacgattgt gtgtgcacg tgctggcaa 
      agatctggt ctcgagtct tgtgtctgg gctgttgac 
      cattgggga ccggtttgg gggcgcgct agatggcgc 
      tgccaagca gttcagtga ggcgtatga cacgggcat 
      gcacccggc cgagtttgt taaccatat gaggaataa 
      aggacagtt gattatggg gatagggca cagggtcaa 
      gtctattaa taatccaga tcaaagggt gaaaattgt 
      caaggagtt cgtgatggc gaatttccc ggcgacgcc 
      actgctgtt gtacgcctt ggaagtgga gaagatcac 
      aacgagcaa aaaaccgaa cttgattct gaacgttga 
      tggggtgat tgcatgctc gtttgtgga catgctgcg 
      gaattgtgg cagttttac cagtgagga ggctcaaga 
      gtacataaa tattggcgc aattaactc ccttttcgt 
      gctggggcg ctcgatagg gttcattgg ccacttcat 
      ggaccagaa gaggctgaa gcaagggtt gtacagaca 
      cccatggga tgatatttc ctacgtact tccagagca 
      atataacaa ctaagttat ttggtttaa gtttttatt 
      aattgctgg tttaattcg cgtgagttt tgtgttgtc 
      tcaatcatt ttgactaaa tcttttcct taatttatt 
      aatggatat ttatatttt tatatttat attaagacg 
      tttccttgt gatttcaca gttaggtaa gagtgtgtc 
      ttatgtact gtcaaaaat aaagttacg ataagtca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY