| General information | |||
| TC015096 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 3356730..3372514 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015096 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02402 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC015096 RA
MSAKAIREA TGKNLINKH LPGGTAAVQ CRFASVEPE TKFSELVAT HPWLKSEKL VVKPDQLIK RRGKLGLIA VNKDLAEVQ QWISDRMNK EQKIGAATG KLRNFIIEP FVPHKDNEE VYVCIYSHR HGDTILFYH QGGVDIGDV DSKAVKLEV PVGESVDEK RITEALLSQ VDASKKAMI ANFVNNLYK LYVDLYFTY LEINPLVVT QKEIYVLDL AAKLDATAD FICKPMWGE IDYPPPFGR DAYPEEAYI ADLDSKSGA SLKLTILNK SGRIWTMVA GGGASVIYS DTICDLGGA KELANYGEY SGAPSEQQT YEYAKTILS LMTQEKHPQ GKVLITGGG IANFTNVAA TFRGIITAL VEFQQRLIE HKVSIFVRR AGPNYQEGL RRMREVGQT LGIPLYVFG PETHMTAIC GMALGTRPI PQENNVEFA TANFLLPSG EKEPNKKSL GVEGVDGGV AAAAACSRK IQGDSLGVQ TPMFTNKTK SIVWGMQTR AVQSMLDFD FVCSRPEPS VVAIIYPFT GDHKQKFYW GHKEILIPV YKKMNDAMT KHPEADVLV NFASLRSAY QSTVETMEF PQIRTIAII AEGIPENQT RKLIKLANE KKVAIIGPA TVGGVKPGC FKIGNTGGM MDNILHSKL YRSGSVAYV SRSGGMSNE LNNIVSKAT DGVYEGVAI GGDRYPGTT FMDHIMRYQ GDENVKMII LLGEVGGVE EYEVCNALK NKIITKPLV AWCIGTCAG MFTSEVQFG HAGSCANSN QETATAKNE ALKAAGAHV PASFDYLGE VIQEVYEKL VIQGVIIPR AEVPPPTVP MDYSWAREL GLIRKPASF MTSICDERG QELIYAGMP ISDVLQKNV GIGGVISLL WFQRCLPTY VCKFFEMCL MVTADHGPA VSGAHNTIV CARAGKDLV SSLVSGLLT IGDRFGGAL DGAAKQFSE AYDTGMHPA EFVNHMRNK GQLIMGIGH RVKSINNPD QRVKIVKEF VMANFPATP LLLYALEVE KITTSKKPN LILNVDGVI ACSFVDMLR NCGSFTSEE AQEYINIGA INSLFVLGR SIGFIGHFM DQKRLKQGL YRHPWDDIS YVLPEQYNN
- >TC015096 RA
atgtcggcg aaggcaata agagaagcg accggaaaa aatctaatc aacaaacac ttacctggc ggtaccgcc gctgtgcaa tgtcgcttc gcttcggtc gaacccgaa acgaaattt tccgaatta gtggccaca catccatgg ttgaaaagc gaaaagttg gttgtgaaa cctgaccaa cttatcaaa cggagagga aaattaggt ttgatcgct gtcaataaa gatttagcc gaagtgcag caatggata agcgatcgt atgaacaaa gagcaaaaa atcggtgcc gccaccggc aaacttcgc aattttatc atcgaacct tttgttcct cacaaagac aacgaagaa gtctatgtt tgtatttat tcgcaccga catggcgat acgatcctg ttttatcat caaggtggc gttgatatc ggtgatgtt gattcgaaa gctgtgaaa ttggaggtt cctgtgggt gaaagtgtc gatgaaaag cgcatcacg gaggctttg ttgagtcaa gttgatgcg agtaaaaag gcgatgatt gcgaatttt gtgaataat ttgtacaag ttgtacgtt gatttgtat tttacttat ttggaaatt aatccattg gtcgtcacc cagaaggag atttatgtt ttggatttg gcggctaag ttggacgct acggctgat tttatttgt aagccaatg tggggcgag attgattat ccgccgccg tttggacgt gatgcatat ccagaggag gcctacatt gccgacctt gattcaaaa tcgggcgcc agtctcaag ctcacaatt ttgaataaa tcgggacgg atttggaca atggtggct gggggcggc gcctccgtg atttacagc gacacgatt tgcgatcta gggggcgcc aaagagctg gcaaattat ggcgaatat tcgggggcg ccttccgaa caacaaacc tacgaatat gccaagacg attctctcg cttatgacg caagagaag cacccccag ggtaaagtt ttgataact gggggtggg attgcgaat tttacaaat gtggcggcg acttttcgc ggtattatc accgctttg gtggagttc caacaacgt ttgatcgag cacaaagtc tcgattttc gtccggaga gctggaccg aattatcaa gagggcttg cggcggatg agggaggtt ggccagact ttggggatt cccctgtac gtgttcggg cctgagaca cacatgacg gccatttgt ggcatggcg ctgggcacg cgccccatt ccacaggag aataacgtt gaatttgca acggctaat tttttgctg cccagtggt gagaaagag ccgaataag aagagtttg ggggtggaa ggggtggat gggggggtg gcggctgcc gccgcttgc agtaggaaa atacaaggc gactcattg ggcgtccaa acacccatg tttaccaac aaaacgaaa tcaatcgtt tggggaatg caaacacga gccgtgcaa agcatgctt gatttcgac ttcgtgtgc agtcggccg gaaccgagc gttgtcgcc attatctat ccatttacc ggggatcac aagcagaaa ttctattgg ggacataag gaaattcta attccagtt tataaaaaa atgaacgac gctatgacg aagcatccc gaagccgac gtccttgtc aacttcgcg tcgcttcgt tccgcttat caaagcacc gtcgaaacg atggaattc ccccaaatc cgaacaatc gcgatcatt gccgaaggc atccccgaa aatcaaacc cgcaaactg atcaaacta gccaatgag aagaaggtt gcaattatt ggacctgcc acagtgggc ggagtcaag ccagggtgt ttcaaaatt ggcaacact ggcggaatg atggataat attctacat tcgaaattg taccgctct ggaagtgtg gcttatgtt tcaaggtca ggtgggatg tccaatgag cttaataat attgtatcg aaagccacc gatggtgtg tatgagggc gtggccata ggcggggat aggtatccc ggtaccacc ttcatggac catattatg cgttaccaa ggcgatgag aacgtcaaa atgattatt ttattgggc gaagtgggc ggagttgaa gagtatgaa gtctgcaac gcccttaag aataaaatt attaccaaa ccgttagtg gcctggtgt attgggacc tgtgcgggc atgttcacc tccgaagtc caattcggc cacgccggc tcttgcgcc aactcgaac caagaaaca gcaacggcc aaaaacgag gccttaaag gctgccggt gcccacgtc ccagcctcc tttgattac ctcggcgag gtgattcaa gaagtttac gaaaagctg gtaatacag ggcgtgata atcccacgt gcggaagtg ccgccacct accgtcccg atggactac tcgtgggca cgtgaactg ggcctaatt cgcaaaccg gccagtttt atgacgtca atttgtgac gaaagaggc caagaattg atctatgca ggcatgcca atctccgac gttttacaa aaaaatgta ggcattggt ggtgtgatt tccctgttg tggttccag cgttgttta ccaacttat gtttgcaaa tttttcgaa atgtgtctg atggtgacg gccgatcat gggcctgcg gtctctggg gcccataac acgattgtg tgtgcacgt gctggcaaa gatctggtc tcgagtctt gtgtctggg ctgttgacc attggggac cggtttggg ggcgcgcta gatggcgct gccaagcag ttcagtgag gcgtatgac acgggcatg cacccggcc gagtttgtt aaccatatg aggaataaa ggacagttg attatgggg atagggcac agggtcaag tctattaat aatccagat caaagggtg aaaattgtc aaggagttc gtgatggcg aatttcccg gcgacgcca ctgctgttg tacgccttg gaagtggag aagatcaca acgagcaaa aaaccgaac ttgattctg aacgttgat ggggtgatt gcatgctcg tttgtggac atgctgcgg aattgtggc agttttacc agtgaggag gctcaagag tacataaat attggcgca attaactcc cttttcgtg ctggggcgc tcgataggg ttcattggc cacttcatg gaccagaag aggctgaag caagggttg tacagacac ccatgggat gatatttcc tacgtactt ccagagcaa tataacaac taa
- >TC015096 RA
acgcgtttt tgaccagtc aaaactctg aagtgacac aagtttgct gtttgtagt ttcaactcg aaaattggg gtaattaac ccactttta gtaattctg tgactgaaa tttgaccca attcttgga aaattccgt gtttttggc atcgaatta aactagaaa aatgtcggc gaaggcaat aagagaagc gaccggaaa aaatctaat caacaaaca cttacctgg cggtaccgc cgctgtgca atgtcgctt cgcttcggt cgaacccga aacgaaatt ttccgaatt agtggccac acatccatg gttgaaaag cgaaaagtt ggttgtgaa acctgacca acttatcaa acggagagg aaaattagg tttgatcgc tgtcaataa agatttagc cgaagtgca gcaatggat aagcgatcg tatgaacaa agagcaaaa aatcggtgc cgccaccgg caaacttcg caattttat catcgaacc ttttgttcc tcacaaaga caacgaaga agtctatgt ttgtattta ttcgcaccg acatggcga tacgatcct gttttatca tcaaggtgg cgttgatat cggtgatgt tgattcgaa agctgtgaa attggaggt tcctgtggg tgaaagtgt cgatgaaaa gcgcatcac ggaggcttt gttgagtca agttgatgc gagtaaaaa ggcgatgat tgcgaattt tgtgaataa tttgtacaa gttgtacgt tgatttgta ttttactta tttggaaat taatccatt ggtcgtcac ccagaagga gatttatgt tttggattt ggcggctaa gttggacgc tacggctga ttttatttg taagccaat gtggggcga gattgatta tccgccgcc gtttggacg tgatgcata tccagagga ggcctacat tgccgacct tgattcaaa atcgggcgc cagtctcaa gctcacaat tttgaataa atcgggacg gatttggac aatggtggc tgggggcgg cgcctccgt gatttacag cgacacgat ttgcgatct agggggcgc caaagagct ggcaaatta tggcgaata ttcgggggc gccttccga acaacaaac ctacgaata tgccaagac gattctctc gcttatgac gcaagagaa gcaccccca gggtaaagt tttgataac tgggggtgg gattgcgaa ttttacaaa tgtggcggc gacttttcg cggtattat caccgcttt ggtggagtt ccaacaacg tttgatcga gcacaaagt ctcgatttt cgtccggag agctggacc gaattatca agagggctt gcggcggat gagggaggt tggccagac tttggggat tcccctgta cgtgttcgg gcctgagac acacatgac ggccatttg tggcatggc gctgggcac gcgccccat tccacagga gaataacgt tgaatttgc aacggctaa ttttttgct gcccagtgg tgagaaaga gccgaataa gaagagttt gggggtgga aggggtgga tgggggggt ggcggctgc cgccgcttg cagtaggaa aatacaagg cgactcatt gggcgtcca aacacccat gtttaccaa caaaacgaa atcaatcgt ttggggaat gcaaacacg agccgtgca aagcatgct tgatttcga cttcgtgtg cagtcggcc ggaaccgag cgttgtcgc cattatcta tccatttac cggggatca caagcagaa attctattg gggacataa ggaaattct aattccagt ttataaaaa aatgaacga cgctatgac gaagcatcc cgaagccga cgtccttgt caacttcgc gtcgcttcg ttccgctta tcaaagcac cgtcgaaac gatggaatt cccccaaat ccgaacaat cgcgatcat tgccgaagg catccccga aaatcaaac ccgcaaact gatcaaact agccaatga gaagaaggt tgcaattat tggacctgc cacagtggg cggagtcaa gccagggtg tttcaaaat tggcaacac tggcggaat gatggataa tattctaca ttcgaaatt gtaccgctc tggaagtgt ggcttatgt ttcaaggtc aggtgggat gtccaatga gcttaataa tattgtatc gaaagccac cgatggtgt gtatgaggg cgtggccat aggcgggga taggtatcc cggtaccac cttcatgga ccatattat gcgttacca aggcgatga gaacgtcaa aatgattat tttattggg cgaagtggg cggagttga agagtatga agtctgcaa cgcccttaa gaataaaat tattaccaa accgttagt ggcctggtg tattgggac ctgtgcggg catgttcac ctccgaagt ccaattcgg ccacgccgg ctcttgcgc caactcgaa ccaagaaac agcaacggc caaaaacga ggccttaaa ggctgccgg tgcccacgt cccagcctc ctttgatta cctcggcga ggtgattca agaagttta cgaaaagct ggtaataca gggcgtgat aatcccacg tgcggaagt gccgccacc taccgtccc gatggacta ctcgtgggc acgtgaact gggcctaat tcgcaaacc ggccagttt tatgacgtc aatttgtga cgaaagagg ccaagaatt gatctatgc aggcatgcc aatctccga cgttttaca aaaaaatgt aggcattgg tggtgtgat ttccctgtt gtggttcca gcgttgttt accaactta tgtttgcaa atttttcga aatgtgtct gatggtgac ggccgatca tgggcctgc ggtctctgg ggcccataa cacgattgt gtgtgcacg tgctggcaa agatctggt ctcgagtct tgtgtctgg gctgttgac cattgggga ccggtttgg gggcgcgct agatggcgc tgccaagca gttcagtga ggcgtatga cacgggcat gcacccggc cgagtttgt taaccatat gaggaataa aggacagtt gattatggg gatagggca cagggtcaa gtctattaa taatccaga tcaaagggt gaaaattgt caaggagtt cgtgatggc gaatttccc ggcgacgcc actgctgtt gtacgcctt ggaagtgga gaagatcac aacgagcaa aaaaccgaa cttgattct gaacgttga tggggtgat tgcatgctc gtttgtgga catgctgcg gaattgtgg cagttttac cagtgagga ggctcaaga gtacataaa tattggcgc aattaactc ccttttcgt gctggggcg ctcgatagg gttcattgg ccacttcat ggaccagaa gaggctgaa gcaagggtt gtacagaca cccatggga tgatatttc ctacgtact tccagagca atataacaa ctaagttat ttggtttaa gtttttatt aattgctgg tttaattcg cgtgagttt tgtgttgtc tcaatcatt ttgactaaa tcttttcct taatttatt aatggatat ttatatttt tatatttat attaagacg tttccttgt gatttcaca gttaggtaa gagtgtgtc ttatgtact gtcaaaaat aaagttacg ataagtca
