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Gene information
General information
TC015095 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3372367..3374774
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015095
    iBeetle screen iB_02401

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 12.5% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands colour darker - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015095 RA
      MTREHQIKL EEGDEKNDS ALILTNEKP VYSIPWRSS 
      LRQIVACCV AHSLVIQAG INMSFSAIL LPQLNEKSS 
      DIHISKSEA SWIASIVAI ALPAGSLII GPLMDRFGR 
      KTLCICTTI PFAISWIIH AAAKSVWHL YLARIIAGF 
      SGGLTTVAL VYVSEITHP NYRTMLLSL NSVFVSFGI 
      LFTCVLGLW FPWRVIATI NCFLVLATL ILLWFLPES 
      PHWYTVFKN KPDQAAKSL EWLYKDPQI FENQLRLLD 
      TSAKNRRKS RIDWSFYKE SVVYKPFFI LFVIFVIQQ 
      LSCGYVIIF YAVDLFREI GGHFRNGLD EFVALVLLG 
      SIRFVMSII SALISKRVG RRPLFFVSG LGQCLTSLV 
      AGVYMYFTV IPPDELAKL SIHKDKGDN IALYCVLGY 
      VCFSSLGYL VIPWTLIGE LFPVKVRGV LGGLMVSIA 
      YIFMFVAVK IFPFVLDLI KIQCVFYVM AVVNLCGVI 
      FIFFFLPET LGKTFNDIE AYFKRVG
    CDS sequence
    • >TC015095 RA
      atgactcgc gaacaccaa atcaaactc gaagaaggc 
      gatgaaaaa aacgacagt gccttgatt ttaaccaac 
      gaaaagccg gtttattca atcccatgg cgctcttcc 
      ctccgacag atcgtagcg tgctgtgtc gcacacagc 
      cttgtgatc caagcaggc attaatatg tcgttcagc 
      gccatctta ctaccgcag ctgaacgaa aagtcgagt 
      gatatacat atttccaaa tcagaggcg tcttggatc 
      gcaagtata gtcgcaata gcccttcca gcgggttcg 
      ttgatcatc ggcccgctt atggaccgc ttcgggcgc 
      aaaactttg tgtatttgt acgactata cccttcgcc 
      atctcgtgg ataatccat gcggcggcg aaaagcgtc 
      tggcatttg tatttagct cggattata gccggcttt 
      agcggcggc ttaacaacc gtagctttg gtatatgtg 
      agcgaaatc actcaccca aactacaga acaatgtta 
      ttgagcctt aacagcgtt tttgtctcg tttggaatt 
      ctttttact tgcgtttta ggtttatgg tttccatgg 
      cgcgtcata gccaccatc aattgtttc ctggtcttg 
      gccaccttg atcctcttg tggttttta cccgagagc 
      cctcactgg tacacagtt ttcaaaaac aagccagac 
      caggctgcc aaatcactt gagtggctc tacaaggac 
      ccacaaatt ttcgaaaat cagttgaga ctattggat 
      acaagtgcc aaaaatcgc cgaaagtca cgaatcgat 
      tggagtttt tacaaagaa tcggttgtc tataagccg 
      tttttcatt ctttttgtt attttcgtg atccagcaa 
      ttgtcgtgc ggttatgtt attattttt tacgccgtt 
      gatttgttt cgggaaatt gggggccat tttcgtaac 
      ggtttggat gaattcgtt gcgttggtt cttctaggt 
      tcgattagg tttgttatg tcgataatt tcggcgcta 
      atttcgaaa cgtgtgggc cgaaggccc ctatttttt 
      gtttcgggg ttaggccaa tgtcttact agtctagtg 
      gccggggtt tatatgtat ttcacggtg attcccccg 
      gacgagttg gccaaactg agtattcac aaggataag 
      ggcgacaat atcgccctc tattgtgtg ttgggttat 
      gtttgcttc agttctttg ggttatttg gtgattccg 
      tggacgcta atcggggaa ttgttcccc gttaaggtc 
      cgcggcgtc ttaggaggc cttatggtt tcgatagct 
      tacattttt atgtttgtt gcggttaaa atcttcccg 
      tttgtgctc gatttgatt aaaattcaa tgcgtcttt 
      tatgtcatg gctgtggtg aatttatgc ggtgttatt 
      ttcatattc ttcttccta cccgaaacg ttaggaaaa 
      acgtttaac gatattgaa gcttatttt aaacgagtt 
      ggttaa
    mRNA sequence
    • >TC015095 RA
      atggggcca agtcctaaa actgggctt aaattttaa 
      taaaaacaa ttgtgtcgc gccttcggc ggcgaacgc 
      acttcgctg gacaaaaat aattaaggg tggtcaccc 
      ctaactcaa aaacgtgac caagtgcca aaaaacggc 
      gcaaaaatt caaattgtg tgacgctca atcattttg 
      tagacggaa cgtcatact agacaaaaa acacaagat 
      aagataacc cccagtggt tcaattggg gggattaaa 
      atttcacgg aactaatat cacagttta aagctgggt 
      cgagtgaca taacacttt tccagtctt tgtttcgct 
      ggtaggaag tagtgaaac agcaatgtc acattgtta 
      tcattcaag tcgtccacg catttactg cgtcgcgaa 
      ttaagcttt tgtctcaat gtaaccatt tcgtaatct 
      aattgacaa aatgataaa acgaattct tttttctat 
      tattcgtcc acaatgact cgcgaacac caaatcaaa 
      ctcgaagaa ggcgatgaa aaaaacgac agtgccttg 
      attttaacc aacgaaaag ccggtttat tcaatccca 
      tggcgctct tccctccga cagatcgta gcgtgctgt 
      gtcgcacac agccttgtg atccaagca ggcattaat 
      atgtcgttc agcgccatc ttactaccg cagctgaac 
      gaaaagtcg agtgatata catatttcc aaatcagag 
      gcgtcttgg atcgcaagt atagtcgca atagccctt 
      ccagcgggt tcgttgatc atcggcccg cttatggac 
      cgcttcggg cgcaaaact ttgtgtatt tgtacgact 
      atacccttc gccatctcg tggataatc catgcggcg 
      gcgaaaagc gtctggcat ttgtattta gctcggatt 
      atagccggc tttagcggc ggcttaaca accgtagct 
      ttggtatat gtgagcgaa atcactcac ccaaactac 
      agaacaatg ttattgagc cttaacagc gtttttgtc 
      tcgtttgga attcttttt acttgcgtt ttaggttta 
      tggtttcca tggcgcgtc atagccacc atcaattgt 
      ttcctggtc ttggccacc ttgatcctc ttgtggttt 
      ttacccgag agccctcac tggtacaca gttttcaaa 
      aacaagcca gaccaggct gccaaatca cttgagtgg 
      ctctacaag gacccacaa attttcgaa aatcagttg 
      agactattg gatacaagt gccaaaaat cgccgaaag 
      tcacgaatc gattggagt ttttacaaa gaatcggtt 
      gtctataag ccgtttttc attcttttt gttattttc 
      gtgatccag caattgtcg tgcggttat gttattatt 
      ttttacgcc gttgatttg tttcgggaa attgggggc 
      cattttcgt aacggtttg gatgaattc gttgcgttg 
      gttcttcta ggttcgatt aggtttgtt atgtcgata 
      atttcggcg ctaatttcg aaacgtgtg ggccgaagg 
      cccctattt tttgtttcg gggttaggc caatgtctt 
      actagtcta gtggccggg gtttatatg tatttcacg 
      gtgattccc ccggacgag ttggccaaa ctgagtatt 
      cacaaggat aagggcgac aatatcgcc ctctattgt 
      gtgttgggt tatgtttgc ttcagttct ttgggttat 
      ttggtgatt ccgtggacg ctaatcggg gaattgttc 
      cccgttaag gtccgcggc gtcttagga ggccttatg 
      gtttcgata gcttacatt tttatgttt gttgcggtt 
      aaaatcttc ccgtttgtg ctcgatttg attaaaatt 
      caatgcgtc ttttatgtc atggctgtg gtgaattta 
      tgcggtgtt attttcata ttcttcttc ctacccgaa 
      acgttagga aaaacgttt aacgatatt gaagcttat 
      tttaaacga gttggttaa gtttaataa atgatttat 
      ttttggatt gtgtccttt ttttactcc ctaccccaa 
      gtgttaatg tccgctgaa cgcgttttt gaccagtca 
      aaactctga agtgacaca agtttgctg tttgtagtt 
      tcaactcga aaattgggg taattaacc cacttttag 
      taattctgt gactgaaat ttgacccaa ttcttggaa 
      aattccgtg tttttggca tcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY