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Gene information
General information
TC015093 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3442759..3447014
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015093
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02399

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015093 RA
      MATLERLQI SGVRSFGPN EEHCQTIKF ATPLTLILG 
QNGSGKTTI IEAIKYVCT AELPQGTNG GQGFVNDPK 
MSSLTTTRG QIKLRVVDS KNNAVTISR IMELTQTAI 
NTQRFSSKG ATIRIVKPN GDDSSISGR CADITNECC 
QIMNVSSSI LNNVVFCHQ ENSAWPLDE GKKVKEKFD 
EIFDAQQSN KCVEIYRKL LKEKQEKIK LLKLELEYK 
KEKKEQVDK DKRVLQDKE AKLESFDAE IAQKMTKLQ 
PVKKRIDEI IDLEKVLSE LERDLATKE ATKNGLVEE 
QKTIKKNLA FEFEGTDQE LQDKIKSFE NERQKDETL 
IQDLVKRQN DIETKKREI NEAVQKTQS DLGRLNQQK 
SHNLKKCCE RKKLFDQAL DKFRVKISN FDDNTGAKH 
AINQLKSAL NERQDDLGT LIEEKELDL MSLQATLDD 
VRAKSIKTQ QIISSKTRE VTDCEQKIE SVSLELAAL 
ASSDEFLQD YTKRLEAIS KTIFNLNNS FRETEESER 
MEASRSQVT TLEHNLDGL EREYRILLQ NDTVEGKIE 
SERHLIMEK QREINKIKS KHAESLREL FGTQSPDCN 
LKDAVLAKQ KSADLALQT LNEQIARKQ KDVTTLEVQ 
LQNQIERIG SCQRDLKTG REKISQVCN GRDFDEVLT 
RCSEKKERL QRDKGNYTS VKIIYNQYI KKFEEERPC 
CPVCETNFS GKTTVVGKI ITTLKSKLD KVPQQLAKV 
ETELVEEEA LYNKLQQLK VVNDEITVL TREKLPELE 
SGLNEIKRD YEAKKTDLE ALKQQTCGP LKTLEISKS 
VISDVSTLD QNQADVDKS QKTVEKLKR ELALVPSQK 
SKQEVEAEI DSTKAELSE LRRKIEAQT KKINSHKDR 
LQQLTQERN ALFEKQIRM KDSMQNRPN LEAQLNEAN 
EKLQTLRGE IVAKKTELE DLEDELGKI ETQKKNLVQ 
ENKTVVEKE RNQVAMFGN LVHNIEKLE GEIEDYVRE 
GVDEKLAAL ETTLGEFRQ KEATLDHTK TKILNLLSE 
KREALAKHE LRFRTLQSN VTLREKKVL ELDLVLKIE 
DLRKLIGGH DYRTVYDEK CELIRQKES HEKTINSIT 
GEKNALLET VDDLRLKLA KPDWKNAYS NYMTKLYEL 
KLAECVVKD LFKYITVLE KAILEFHAT RMKQINKTI 
REMWREIYR GNDVDYIEI KAEHAGSTT ANRKRTYNY 
RVVQVKKGV ELEMRGRCS AGQKVLACL IIRMALAET 
LSANCGILA LDEPTTNLD RENIFSLCE ALARIVESR 
QKEKNFQLV VITHDEEFI NALTRAQGV PFFYRVSRN 
QDGFSVIKK EYAL
    CDS sequence
    • >TC015093 RA
      atggcaact ttagaacgc ctccaaatc tccggcgtt 
cgcagcttt ggccccaac gaagaacac tgccaaaca 
ataaaattt gcgacccct ttaacccta attctgggc 
cagaacggt tccggcaaa accactata atcgaagcc 
atcaagtac gtatgtacc gccgaactg ccccaaggg 
acaaatggg ggccaaggg ttcgtcaac gaccccaaa 
atgtcgtcc ctaaccacc acaaggggc caaattaaa 
ctacgcgtt gttgactcg aaaaacaac gcggttacg 
attagtcgc atcatggaa ttaacccaa actgcaatc 
aacacgcaa cggttcagc tcaaaaggg gcgacaatc 
cgtatcgtg aaaccaaac ggcgacgac tcgagcatt 
tccggccgt tgtgccgat attacaaac gagtgttgt 
caaataatg aacgtttcg tcttcgatt ctcaataat 
gtggtgttc tgtcaccaa gaaaactcc gcctggcct 
ttggacgaa ggcaagaaa gtcaaggag aagtttgac 
gagattttc gacgcccaa cagtccaac aaatgcgtg 
gaaatttac cgtaaactg ctcaaagaa aagcaagag 
aaaatcaag cttcttaaa ctcgaactt gaatacaaa 
aaagagaag aaagaacaa gtggataaa gacaagcga 
gtcttgcaa gacaaagag gcgaaactt gagtcattt 
gacgccgaa atcgcgcaa aaaatgacg aaactacaa 
ccggtcaag aaacgaatc gatgaaatt atcgacctg 
gagaaggtc ctaagtgag ctagaacgc gatttggcg 
actaaggaa gccacgaaa aatgggcta gttgaagag 
caaaaaacc atcaagaaa aatctagct tttgagttc 
gaaggcacg gatcaagaa ctacaagac aagatcaaa 
tctttcgaa aacgagcgt caaaaggac gaaacgttg 
atacaagat ttagtcaaa cgccagaac gacattgaa 
accaaaaag cgggagatt aacgaagcg gtgcagaaa 
acacaaagt gacttggga cgcttgaac caacagaaa 
agtcacaac ttgaagaag tgttgcgaa cgcaaaaaa 
ttattcgac caggctttg gacaagttc cgtgtgaag 
atatcgaac tttgacgac aataccgga gccaaacat 
gcaataaat cagctaaag tccgctcta aatgagcgc 
caagacgat ctggggaca cttattgaa gagaaagaa 
ttggacttg atgtcccta caagccaca cttgatgac 
gtccgtgcc aaaagcatc aaaacccaa caaatcatt 
tcgtcaaaa acgcgagaa gtcaccgat tgtgagcaa 
aaaatcgaa agtgtttca ctcgagttg gccgcactt 
gcctcctct gatgagttc cttcaagac tacacgaaa 
cgtctggag gccatcagt aagacaatc ttcaacttg 
aacaactcg ttccgtgaa accgaagaa agcgaacga 
atggaagct agtcggagt caagtcacg accttggaa 
cacaatcta gacggcttg gagcgggag tatcgcatc 
ctcctccag aatgatacc gttgaggga aaaatcgag 
agtgagagg cacctaatt atggaaaaa caacgggag 
attaacaag attaaaagc aaacatgcg gagagtctt 
cgagaattg tttgggaca caatcgcct gactgcaat 
ttgaaagac gccgttttg gcgaaacag aaaagtgca 
gatctggcg cttcaaaca ctcaatgag caaatcgcg 
cggaaacaa aaggacgtg accacgctt gaagtgcaa 
ctacagaac caaatcgaa aggattggt tcctgccaa 
cgggacttg aaaaccggc cgtgagaaa atatcacaa 
gtgtgcaac ggacgcgac tttgacgaa gtcctaacc 
cgttgttcc gaaaaaaag gaacgctta cagcgggat 
aaggggaat tacacttcg gttaaaatt atctacaac 
caatacatt aagaagttt gaagaggag aggccgtgt 
tgtcccgtt tgtgagaca aatttttcg gggaaaacc 
acagtcgtc ggtaagatt ataacaacg ttgaaaagt 
aaactcgat aaagtccct caacaactg gcgaaggtt 
gaaaccgag ttagttgaa gaggaggcg ttgtacaac 
aaattgcaa caattgaaa gttgtcaat gacgaaatc 
actgtcttg acgagggaa aagctgccg gagctggaa 
tcgggtctt aacgagatc aagcgcgat tacgaggcg 
aaaaaaacc gacttggag gcgttaaaa caacaaact 
tgtgggcct ttgaagact ttggaaatc tccaaaagt 
gttatctct gatgttagc acgctggat cagaatcaa 
gccgacgtt gacaagtcg caaaagaca gtcgagaag 
ttaaaacgt gagctggca cttgtccct tcacagaaa 
tcgaagcaa gaagtcgag gctgaaatt gactcgacc 
aaggccgag ttgagcgaa ttgaggcgc aagattgag 
gcgcaaacc aagaaaatc aacagccat aaggaccgc 
ttgcagcaa ctgacccag gaacggaac gctctgttt 
gagaagcaa atccggatg aaggacagt atgcaaaac 
cggccaaac cttgaagca cagttgaac gaagcgaac 
gagaaactc caaactttg aggggggaa atagtggcg 
aaaaagacc gaactggag gatttggaa gacgagttg 
gggaaaatc gagacgcag aaaaaaaac ctcgttcag 
gagaacaaa actgtcgtt gagaaggaa cgaaaccaa 
gtggccatg tttggtaac ctagtccac aacattgag 
aaactggaa ggcgaaatt gaggattat gtgcgcgaa 
ggtgttgac gaaaagttg gcggcgctt gaaactacc 
ttgggggag tttcgccaa aaagaggcg actcttgat 
cacacgaaa acgaaaatt ttgaattta ctgtcggag 
aagagggag gctttggcg aaacatgag ctgcgcttc 
cggacgttg caaagcaac gtaactttg cgcgagaaa 
aaggtgctt gagttggat ttggtgttg aaaattgag 
gatttgagg aagttgatc ggtgggcat gactaccgt 
acggtctac gatgagaag tgtgaattg attaggcaa 
aaagagagt cacgaaaag accattaat agtataact 
ggtgagaag aacgcgctt ttggagacg gttgacgat 
ttgcggctc aaactggca aagcccgat tggaaaaat 
gcctacagt aactatatg acaaagttg tatgaactc 
aaactggcc gaatgcgtg gttaaagac ttgttcaag 
tacatcacg gtcctcgag aaggccatt ctggagttc 
cacgctacg cgaatgaag caaatcaat aaaacaatc 
cgcgaaatg tggcgtgag atatacagg gggaacgac 
gttgattac attgagatc aaagccgag cacgctggc 
tccacaacg gccaatagg aaacgcacc tataactac 
agagtggtt caggtgaag aagggagtg gagttggag 
atgcggggg cgctgcagt gccggtcag aaagtccta 
gcttgtctc attatacgt atggctttg gctgagacg 
ttgagtgcc aattgtggg attttggcg cttgatgaa 
ccgacgact aatttagat agggagaat atctttagt 
ttgtgcgaa gctttggcc aggattgtg gagtcgagg 
cagaaagag aagaatttt caactggtg gttattacg 
cacgatgag gagttcatt aatgcgctt acgagggca 
caaggtgtg ccctttttc tacagggtg tcgcggaac 
caggacggg ttctcggtc attaagaag gaatacgcg 
ttgtaa
    mRNA sequence
    • >TC015093 RA
      taacctaac tttcgatcg cgttaaaat agcaaacat 
tagccgaaa aacactaat gtgagcgct tatttcata 
accagtgtg ttactcaac acttgtgct aactattct 
ttatcaaat ttgcaagtg ttacgagtt ttttttaac 
ccaaaaact tgtaaactc gtcaagact tgcattaac 
cctcaatga cacccaact accacattt tttaaatca 
gcgatggca actttagaa cgcctccaa atctccggc 
gttcgcagc tttggcccc aacgaagaa cactgccaa 
acaataaaa tttgcgacc cctttaacc ctaattctg 
ggccagaac ggttccggc aaaaccact ataatcgaa 
gccatcaag tacgtatgt accgccgaa ctgccccaa 
gggacaaat gggggccaa gggttcgtc aacgacccc 
aaaatgtcg tccctaacc accacaagg ggccaaatt 
aaactacgc gttgttgac tcgaaaaac aacgcggtt 
acgattagt cgcatcatg gaattaacc caaactgca 
atcaacacg caacggttc agctcaaaa ggggcgaca 
atccgtatc gtgaaacca aacggcgac gactcgagc 
atttccggc cgttgtgcc gatattaca aacgagtgt 
tgtcaaata atgaacgtt tcgtcttcg attctcaat 
aatgtggtg ttctgtcac caagaaaac tccgcctgg 
cctttggac gaaggcaag aaagtcaag gagaagttt 
gacgagatt ttcgacgcc caacagtcc aacaaatgc 
gtggaaatt taccgtaaa ctgctcaaa gaaaagcaa 
gagaaaatc aagcttctt aaactcgaa cttgaatac 
aaaaaagag aagaaagaa caagtggat aaagacaag 
cgagtcttg caagacaaa gaggcgaaa cttgagtca 
tttgacgcc gaaatcgcg caaaaaatg acgaaacta 
caaccggtc aagaaacga atcgatgaa attatcgac 
ctggagaag gtcctaagt gagctagaa cgcgatttg 
gcgactaag gaagccacg aaaaatggg ctagttgaa 
gagcaaaaa accatcaag aaaaatcta gcttttgag 
ttcgaaggc acggatcaa gaactacaa gacaagatc 
aaatctttc gaaaacgag cgtcaaaag gacgaaacg 
ttgatacaa gatttagtc aaacgccag aacgacatt 
gaaaccaaa aagcgggag attaacgaa gcggtgcag 
aaaacacaa agtgacttg ggacgcttg aaccaacag 
aaaagtcac aacttgaag aagtgttgc gaacgcaaa 
aaattattc gaccaggct ttggacaag ttccgtgtg 
aagatatcg aactttgac gacaatacc ggagccaaa 
catgcaata aatcagcta aagtccgct ctaaatgag 
cgccaagac gatctgggg acacttatt gaagagaaa 
gaattggac ttgatgtcc ctacaagcc acacttgat 
gacgtccgt gccaaaagc atcaaaacc caacaaatc 
atttcgtca aaaacgcga gaagtcacc gattgtgag 
caaaaaatc gaaagtgtt tcactcgag ttggccgca 
cttgcctcc tctgatgag ttccttcaa gactacacg 
aaacgtctg gaggccatc agtaagaca atcttcaac 
ttgaacaac tcgttccgt gaaaccgaa gaaagcgaa 
cgaatggaa gctagtcgg agtcaagtc acgaccttg 
gaacacaat ctagacggc ttggagcgg gagtatcgc 
atcctcctc cagaatgat accgttgag ggaaaaatc 
gagagtgag aggcaccta attatggaa aaacaacgg 
gagattaac aagattaaa agcaaacat gcggagagt 
cttcgagaa ttgtttggg acacaatcg cctgactgc 
aatttgaaa gacgccgtt ttggcgaaa cagaaaagt 
gcagatctg gcgcttcaa acactcaat gagcaaatc 
gcgcggaaa caaaaggac gtgaccacg cttgaagtg 
caactacag aaccaaatc gaaaggatt ggttcctgc 
caacgggac ttgaaaacc ggccgtgag aaaatatca 
caagtgtgc aacggacgc gactttgac gaagtccta 
acccgttgt tccgaaaaa aaggaacgc ttacagcgg 
gataagggg aattacact tcggttaaa attatctac 
aaccaatac attaagaag tttgaagag gagaggccg 
tgttgtccc gtttgtgag acaaatttt tcggggaaa 
accacagtc gtcggtaag attataaca acgttgaaa 
agtaaactc gataaagtc cctcaacaa ctggcgaag 
gttgaaacc gagttagtt gaagaggag gcgttgtac 
aacaaattg caacaattg aaagttgtc aatgacgaa 
atcactgtc ttgacgagg gaaaagctg ccggagctg 
gaatcgggt cttaacgag atcaagcgc gattacgag 
gcgaaaaaa accgacttg gaggcgtta aaacaacaa 
acttgtggg cctttgaag actttggaa atctccaaa 
agtgttatc tctgatgtt agcacgctg gatcagaat 
caagccgac gttgacaag tcgcaaaag acagtcgag 
aagttaaaa cgtgagctg gcacttgtc ccttcacag 
aaatcgaag caagaagtc gaggctgaa attgactcg 
accaaggcc gagttgagc gaattgagg cgcaagatt 
gaggcgcaa accaagaaa atcaacagc cataaggac 
cgcttgcag caactgacc caggaacgg aacgctctg 
tttgagaag caaatccgg atgaaggac agtatgcaa 
aaccggcca aaccttgaa gcacagttg aacgaagcg 
aacgagaaa ctccaaact ttgaggggg gaaatagtg 
gcgaaaaag accgaactg gaggatttg gaagacgag 
ttggggaaa atcgagacg cagaaaaaa aacctcgtt 
caggagaac aaaactgtc gttgagaag gaacgaaac 
caagtggcc atgtttggt aacctagtc cacaacatt 
gagaaactg gaaggcgaa attgaggat tatgtgcgc 
gaaggtgtt gacgaaaag ttggcggcg cttgaaact 
accttgggg gagtttcgc caaaaagag gcgactctt 
gatcacacg aaaacgaaa attttgaat ttactgtcg 
gagaagagg gaggctttg gcgaaacat gagctgcgc 
ttccggacg ttgcaaagc aacgtaact ttgcgcgag 
aaaaaggtg cttgagttg gatttggtg ttgaaaatt 
gaggatttg aggaagttg atcggtggg catgactac 
cgtacggtc tacgatgag aagtgtgaa ttgattagg 
caaaaagag agtcacgaa aagaccatt aatagtata 
actggtgag aagaacgcg cttttggag acggttgac 
gatttgcgg ctcaaactg gcaaagccc gattggaaa 
aatgcctac agtaactat atgacaaag ttgtatgaa 
ctcaaactg gccgaatgc gtggttaaa gacttgttc 
aagtacatc acggtcctc gagaaggcc attctggag 
ttccacgct acgcgaatg aagcaaatc aataaaaca 
atccgcgaa atgtggcgt gagatatac agggggaac 
gacgttgat tacattgag atcaaagcc gagcacgct 
ggctccaca acggccaat aggaaacgc acctataac 
tacagagtg gttcaggtg aagaaggga gtggagttg 
gagatgcgg gggcgctgc agtgccggt cagaaagtc 
ctagcttgt ctcattata cgtatggct ttggctgag 
acgttgagt gccaattgt gggattttg gcgcttgat 
gaaccgacg actaattta gatagggag aatatcttt 
agtttgtgc gaagctttg gccaggatt gtggagtcg 
aggcagaaa gagaagaat tttcaactg gtggttatt 
acgcacgat gaggagttc attaatgcg cttacgagg 
gcacaaggt gtgcccttt ttctacagg gtgtcgcgg 
aaccaggac gggttctcg gtcattaag aaggaatac 
gcgttgtaa cgtggtttt tctcccatt gtttatttt 
ttttaataa aattttgtg gataat
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