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Gene information
General information
TC015093 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3442759..3447014
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015093
    iBeetle screen iB_02399

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015093 RA
      MATLERLQI SGVRSFGPN EEHCQTIKF ATPLTLILG 
      QNGSGKTTI IEAIKYVCT AELPQGTNG GQGFVNDPK 
      MSSLTTTRG QIKLRVVDS KNNAVTISR IMELTQTAI 
      NTQRFSSKG ATIRIVKPN GDDSSISGR CADITNECC 
      QIMNVSSSI LNNVVFCHQ ENSAWPLDE GKKVKEKFD 
      EIFDAQQSN KCVEIYRKL LKEKQEKIK LLKLELEYK 
      KEKKEQVDK DKRVLQDKE AKLESFDAE IAQKMTKLQ 
      PVKKRIDEI IDLEKVLSE LERDLATKE ATKNGLVEE 
      QKTIKKNLA FEFEGTDQE LQDKIKSFE NERQKDETL 
      IQDLVKRQN DIETKKREI NEAVQKTQS DLGRLNQQK 
      SHNLKKCCE RKKLFDQAL DKFRVKISN FDDNTGAKH 
      AINQLKSAL NERQDDLGT LIEEKELDL MSLQATLDD 
      VRAKSIKTQ QIISSKTRE VTDCEQKIE SVSLELAAL 
      ASSDEFLQD YTKRLEAIS KTIFNLNNS FRETEESER 
      MEASRSQVT TLEHNLDGL EREYRILLQ NDTVEGKIE 
      SERHLIMEK QREINKIKS KHAESLREL FGTQSPDCN 
      LKDAVLAKQ KSADLALQT LNEQIARKQ KDVTTLEVQ 
      LQNQIERIG SCQRDLKTG REKISQVCN GRDFDEVLT 
      RCSEKKERL QRDKGNYTS VKIIYNQYI KKFEEERPC 
      CPVCETNFS GKTTVVGKI ITTLKSKLD KVPQQLAKV 
      ETELVEEEA LYNKLQQLK VVNDEITVL TREKLPELE 
      SGLNEIKRD YEAKKTDLE ALKQQTCGP LKTLEISKS 
      VISDVSTLD QNQADVDKS QKTVEKLKR ELALVPSQK 
      SKQEVEAEI DSTKAELSE LRRKIEAQT KKINSHKDR 
      LQQLTQERN ALFEKQIRM KDSMQNRPN LEAQLNEAN 
      EKLQTLRGE IVAKKTELE DLEDELGKI ETQKKNLVQ 
      ENKTVVEKE RNQVAMFGN LVHNIEKLE GEIEDYVRE 
      GVDEKLAAL ETTLGEFRQ KEATLDHTK TKILNLLSE 
      KREALAKHE LRFRTLQSN VTLREKKVL ELDLVLKIE 
      DLRKLIGGH DYRTVYDEK CELIRQKES HEKTINSIT 
      GEKNALLET VDDLRLKLA KPDWKNAYS NYMTKLYEL 
      KLAECVVKD LFKYITVLE KAILEFHAT RMKQINKTI 
      REMWREIYR GNDVDYIEI KAEHAGSTT ANRKRTYNY 
      RVVQVKKGV ELEMRGRCS AGQKVLACL IIRMALAET 
      LSANCGILA LDEPTTNLD RENIFSLCE ALARIVESR 
      QKEKNFQLV VITHDEEFI NALTRAQGV PFFYRVSRN 
      QDGFSVIKK EYAL
    CDS sequence
    • >TC015093 RA
      atggcaact ttagaacgc ctccaaatc tccggcgtt 
      cgcagcttt ggccccaac gaagaacac tgccaaaca 
      ataaaattt gcgacccct ttaacccta attctgggc 
      cagaacggt tccggcaaa accactata atcgaagcc 
      atcaagtac gtatgtacc gccgaactg ccccaaggg 
      acaaatggg ggccaaggg ttcgtcaac gaccccaaa 
      atgtcgtcc ctaaccacc acaaggggc caaattaaa 
      ctacgcgtt gttgactcg aaaaacaac gcggttacg 
      attagtcgc atcatggaa ttaacccaa actgcaatc 
      aacacgcaa cggttcagc tcaaaaggg gcgacaatc 
      cgtatcgtg aaaccaaac ggcgacgac tcgagcatt 
      tccggccgt tgtgccgat attacaaac gagtgttgt 
      caaataatg aacgtttcg tcttcgatt ctcaataat 
      gtggtgttc tgtcaccaa gaaaactcc gcctggcct 
      ttggacgaa ggcaagaaa gtcaaggag aagtttgac 
      gagattttc gacgcccaa cagtccaac aaatgcgtg 
      gaaatttac cgtaaactg ctcaaagaa aagcaagag 
      aaaatcaag cttcttaaa ctcgaactt gaatacaaa 
      aaagagaag aaagaacaa gtggataaa gacaagcga 
      gtcttgcaa gacaaagag gcgaaactt gagtcattt 
      gacgccgaa atcgcgcaa aaaatgacg aaactacaa 
      ccggtcaag aaacgaatc gatgaaatt atcgacctg 
      gagaaggtc ctaagtgag ctagaacgc gatttggcg 
      actaaggaa gccacgaaa aatgggcta gttgaagag 
      caaaaaacc atcaagaaa aatctagct tttgagttc 
      gaaggcacg gatcaagaa ctacaagac aagatcaaa 
      tctttcgaa aacgagcgt caaaaggac gaaacgttg 
      atacaagat ttagtcaaa cgccagaac gacattgaa 
      accaaaaag cgggagatt aacgaagcg gtgcagaaa 
      acacaaagt gacttggga cgcttgaac caacagaaa 
      agtcacaac ttgaagaag tgttgcgaa cgcaaaaaa 
      ttattcgac caggctttg gacaagttc cgtgtgaag 
      atatcgaac tttgacgac aataccgga gccaaacat 
      gcaataaat cagctaaag tccgctcta aatgagcgc 
      caagacgat ctggggaca cttattgaa gagaaagaa 
      ttggacttg atgtcccta caagccaca cttgatgac 
      gtccgtgcc aaaagcatc aaaacccaa caaatcatt 
      tcgtcaaaa acgcgagaa gtcaccgat tgtgagcaa 
      aaaatcgaa agtgtttca ctcgagttg gccgcactt 
      gcctcctct gatgagttc cttcaagac tacacgaaa 
      cgtctggag gccatcagt aagacaatc ttcaacttg 
      aacaactcg ttccgtgaa accgaagaa agcgaacga 
      atggaagct agtcggagt caagtcacg accttggaa 
      cacaatcta gacggcttg gagcgggag tatcgcatc 
      ctcctccag aatgatacc gttgaggga aaaatcgag 
      agtgagagg cacctaatt atggaaaaa caacgggag 
      attaacaag attaaaagc aaacatgcg gagagtctt 
      cgagaattg tttgggaca caatcgcct gactgcaat 
      ttgaaagac gccgttttg gcgaaacag aaaagtgca 
      gatctggcg cttcaaaca ctcaatgag caaatcgcg 
      cggaaacaa aaggacgtg accacgctt gaagtgcaa 
      ctacagaac caaatcgaa aggattggt tcctgccaa 
      cgggacttg aaaaccggc cgtgagaaa atatcacaa 
      gtgtgcaac ggacgcgac tttgacgaa gtcctaacc 
      cgttgttcc gaaaaaaag gaacgctta cagcgggat 
      aaggggaat tacacttcg gttaaaatt atctacaac 
      caatacatt aagaagttt gaagaggag aggccgtgt 
      tgtcccgtt tgtgagaca aatttttcg gggaaaacc 
      acagtcgtc ggtaagatt ataacaacg ttgaaaagt 
      aaactcgat aaagtccct caacaactg gcgaaggtt 
      gaaaccgag ttagttgaa gaggaggcg ttgtacaac 
      aaattgcaa caattgaaa gttgtcaat gacgaaatc 
      actgtcttg acgagggaa aagctgccg gagctggaa 
      tcgggtctt aacgagatc aagcgcgat tacgaggcg 
      aaaaaaacc gacttggag gcgttaaaa caacaaact 
      tgtgggcct ttgaagact ttggaaatc tccaaaagt 
      gttatctct gatgttagc acgctggat cagaatcaa 
      gccgacgtt gacaagtcg caaaagaca gtcgagaag 
      ttaaaacgt gagctggca cttgtccct tcacagaaa 
      tcgaagcaa gaagtcgag gctgaaatt gactcgacc 
      aaggccgag ttgagcgaa ttgaggcgc aagattgag 
      gcgcaaacc aagaaaatc aacagccat aaggaccgc 
      ttgcagcaa ctgacccag gaacggaac gctctgttt 
      gagaagcaa atccggatg aaggacagt atgcaaaac 
      cggccaaac cttgaagca cagttgaac gaagcgaac 
      gagaaactc caaactttg aggggggaa atagtggcg 
      aaaaagacc gaactggag gatttggaa gacgagttg 
      gggaaaatc gagacgcag aaaaaaaac ctcgttcag 
      gagaacaaa actgtcgtt gagaaggaa cgaaaccaa 
      gtggccatg tttggtaac ctagtccac aacattgag 
      aaactggaa ggcgaaatt gaggattat gtgcgcgaa 
      ggtgttgac gaaaagttg gcggcgctt gaaactacc 
      ttgggggag tttcgccaa aaagaggcg actcttgat 
      cacacgaaa acgaaaatt ttgaattta ctgtcggag 
      aagagggag gctttggcg aaacatgag ctgcgcttc 
      cggacgttg caaagcaac gtaactttg cgcgagaaa 
      aaggtgctt gagttggat ttggtgttg aaaattgag 
      gatttgagg aagttgatc ggtgggcat gactaccgt 
      acggtctac gatgagaag tgtgaattg attaggcaa 
      aaagagagt cacgaaaag accattaat agtataact 
      ggtgagaag aacgcgctt ttggagacg gttgacgat 
      ttgcggctc aaactggca aagcccgat tggaaaaat 
      gcctacagt aactatatg acaaagttg tatgaactc 
      aaactggcc gaatgcgtg gttaaagac ttgttcaag 
      tacatcacg gtcctcgag aaggccatt ctggagttc 
      cacgctacg cgaatgaag caaatcaat aaaacaatc 
      cgcgaaatg tggcgtgag atatacagg gggaacgac 
      gttgattac attgagatc aaagccgag cacgctggc 
      tccacaacg gccaatagg aaacgcacc tataactac 
      agagtggtt caggtgaag aagggagtg gagttggag 
      atgcggggg cgctgcagt gccggtcag aaagtccta 
      gcttgtctc attatacgt atggctttg gctgagacg 
      ttgagtgcc aattgtggg attttggcg cttgatgaa 
      ccgacgact aatttagat agggagaat atctttagt 
      ttgtgcgaa gctttggcc aggattgtg gagtcgagg 
      cagaaagag aagaatttt caactggtg gttattacg 
      cacgatgag gagttcatt aatgcgctt acgagggca 
      caaggtgtg ccctttttc tacagggtg tcgcggaac 
      caggacggg ttctcggtc attaagaag gaatacgcg 
      ttgtaa
    mRNA sequence
    • >TC015093 RA
      taacctaac tttcgatcg cgttaaaat agcaaacat 
      tagccgaaa aacactaat gtgagcgct tatttcata 
      accagtgtg ttactcaac acttgtgct aactattct 
      ttatcaaat ttgcaagtg ttacgagtt ttttttaac 
      ccaaaaact tgtaaactc gtcaagact tgcattaac 
      cctcaatga cacccaact accacattt tttaaatca 
      gcgatggca actttagaa cgcctccaa atctccggc 
      gttcgcagc tttggcccc aacgaagaa cactgccaa 
      acaataaaa tttgcgacc cctttaacc ctaattctg 
      ggccagaac ggttccggc aaaaccact ataatcgaa 
      gccatcaag tacgtatgt accgccgaa ctgccccaa 
      gggacaaat gggggccaa gggttcgtc aacgacccc 
      aaaatgtcg tccctaacc accacaagg ggccaaatt 
      aaactacgc gttgttgac tcgaaaaac aacgcggtt 
      acgattagt cgcatcatg gaattaacc caaactgca 
      atcaacacg caacggttc agctcaaaa ggggcgaca 
      atccgtatc gtgaaacca aacggcgac gactcgagc 
      atttccggc cgttgtgcc gatattaca aacgagtgt 
      tgtcaaata atgaacgtt tcgtcttcg attctcaat 
      aatgtggtg ttctgtcac caagaaaac tccgcctgg 
      cctttggac gaaggcaag aaagtcaag gagaagttt 
      gacgagatt ttcgacgcc caacagtcc aacaaatgc 
      gtggaaatt taccgtaaa ctgctcaaa gaaaagcaa 
      gagaaaatc aagcttctt aaactcgaa cttgaatac 
      aaaaaagag aagaaagaa caagtggat aaagacaag 
      cgagtcttg caagacaaa gaggcgaaa cttgagtca 
      tttgacgcc gaaatcgcg caaaaaatg acgaaacta 
      caaccggtc aagaaacga atcgatgaa attatcgac 
      ctggagaag gtcctaagt gagctagaa cgcgatttg 
      gcgactaag gaagccacg aaaaatggg ctagttgaa 
      gagcaaaaa accatcaag aaaaatcta gcttttgag 
      ttcgaaggc acggatcaa gaactacaa gacaagatc 
      aaatctttc gaaaacgag cgtcaaaag gacgaaacg 
      ttgatacaa gatttagtc aaacgccag aacgacatt 
      gaaaccaaa aagcgggag attaacgaa gcggtgcag 
      aaaacacaa agtgacttg ggacgcttg aaccaacag 
      aaaagtcac aacttgaag aagtgttgc gaacgcaaa 
      aaattattc gaccaggct ttggacaag ttccgtgtg 
      aagatatcg aactttgac gacaatacc ggagccaaa 
      catgcaata aatcagcta aagtccgct ctaaatgag 
      cgccaagac gatctgggg acacttatt gaagagaaa 
      gaattggac ttgatgtcc ctacaagcc acacttgat 
      gacgtccgt gccaaaagc atcaaaacc caacaaatc 
      atttcgtca aaaacgcga gaagtcacc gattgtgag 
      caaaaaatc gaaagtgtt tcactcgag ttggccgca 
      cttgcctcc tctgatgag ttccttcaa gactacacg 
      aaacgtctg gaggccatc agtaagaca atcttcaac 
      ttgaacaac tcgttccgt gaaaccgaa gaaagcgaa 
      cgaatggaa gctagtcgg agtcaagtc acgaccttg 
      gaacacaat ctagacggc ttggagcgg gagtatcgc 
      atcctcctc cagaatgat accgttgag ggaaaaatc 
      gagagtgag aggcaccta attatggaa aaacaacgg 
      gagattaac aagattaaa agcaaacat gcggagagt 
      cttcgagaa ttgtttggg acacaatcg cctgactgc 
      aatttgaaa gacgccgtt ttggcgaaa cagaaaagt 
      gcagatctg gcgcttcaa acactcaat gagcaaatc 
      gcgcggaaa caaaaggac gtgaccacg cttgaagtg 
      caactacag aaccaaatc gaaaggatt ggttcctgc 
      caacgggac ttgaaaacc ggccgtgag aaaatatca 
      caagtgtgc aacggacgc gactttgac gaagtccta 
      acccgttgt tccgaaaaa aaggaacgc ttacagcgg 
      gataagggg aattacact tcggttaaa attatctac 
      aaccaatac attaagaag tttgaagag gagaggccg 
      tgttgtccc gtttgtgag acaaatttt tcggggaaa 
      accacagtc gtcggtaag attataaca acgttgaaa 
      agtaaactc gataaagtc cctcaacaa ctggcgaag 
      gttgaaacc gagttagtt gaagaggag gcgttgtac 
      aacaaattg caacaattg aaagttgtc aatgacgaa 
      atcactgtc ttgacgagg gaaaagctg ccggagctg 
      gaatcgggt cttaacgag atcaagcgc gattacgag 
      gcgaaaaaa accgacttg gaggcgtta aaacaacaa 
      acttgtggg cctttgaag actttggaa atctccaaa 
      agtgttatc tctgatgtt agcacgctg gatcagaat 
      caagccgac gttgacaag tcgcaaaag acagtcgag 
      aagttaaaa cgtgagctg gcacttgtc ccttcacag 
      aaatcgaag caagaagtc gaggctgaa attgactcg 
      accaaggcc gagttgagc gaattgagg cgcaagatt 
      gaggcgcaa accaagaaa atcaacagc cataaggac 
      cgcttgcag caactgacc caggaacgg aacgctctg 
      tttgagaag caaatccgg atgaaggac agtatgcaa 
      aaccggcca aaccttgaa gcacagttg aacgaagcg 
      aacgagaaa ctccaaact ttgaggggg gaaatagtg 
      gcgaaaaag accgaactg gaggatttg gaagacgag 
      ttggggaaa atcgagacg cagaaaaaa aacctcgtt 
      caggagaac aaaactgtc gttgagaag gaacgaaac 
      caagtggcc atgtttggt aacctagtc cacaacatt 
      gagaaactg gaaggcgaa attgaggat tatgtgcgc 
      gaaggtgtt gacgaaaag ttggcggcg cttgaaact 
      accttgggg gagtttcgc caaaaagag gcgactctt 
      gatcacacg aaaacgaaa attttgaat ttactgtcg 
      gagaagagg gaggctttg gcgaaacat gagctgcgc 
      ttccggacg ttgcaaagc aacgtaact ttgcgcgag 
      aaaaaggtg cttgagttg gatttggtg ttgaaaatt 
      gaggatttg aggaagttg atcggtggg catgactac 
      cgtacggtc tacgatgag aagtgtgaa ttgattagg 
      caaaaagag agtcacgaa aagaccatt aatagtata 
      actggtgag aagaacgcg cttttggag acggttgac 
      gatttgcgg ctcaaactg gcaaagccc gattggaaa 
      aatgcctac agtaactat atgacaaag ttgtatgaa 
      ctcaaactg gccgaatgc gtggttaaa gacttgttc 
      aagtacatc acggtcctc gagaaggcc attctggag 
      ttccacgct acgcgaatg aagcaaatc aataaaaca 
      atccgcgaa atgtggcgt gagatatac agggggaac 
      gacgttgat tacattgag atcaaagcc gagcacgct 
      ggctccaca acggccaat aggaaacgc acctataac 
      tacagagtg gttcaggtg aagaaggga gtggagttg 
      gagatgcgg gggcgctgc agtgccggt cagaaagtc 
      ctagcttgt ctcattata cgtatggct ttggctgag 
      acgttgagt gccaattgt gggattttg gcgcttgat 
      gaaccgacg actaattta gatagggag aatatcttt 
      agtttgtgc gaagctttg gccaggatt gtggagtcg 
      aggcagaaa gagaagaat tttcaactg gtggttatt 
      acgcacgat gaggagttc attaatgcg cttacgagg 
      gcacaaggt gtgcccttt ttctacagg gtgtcgcgg 
      aaccaggac gggttctcg gtcattaag aaggaatac 
      gcgttgtaa cgtggtttt tctcccatt gtttatttt 
      ttttaataa aattttgtg gataat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY