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Gene information
General information
TC015091 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3470207..3479824
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015091
      iBeetle screen iB_02398

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015091 RA
        MLYETKAQF AAVVALPIV TILLVWTSA IFQRNRKSW 
        GPYDIPIVT LLILSILRN LTVLTYTLF VALSQDAIN 
        HDYCSALVW IFNSIHTFQ ASSLTTLAV IGLFSVKLH 
        RKQQHLRQF LTATHVIYH LFCLTTLCA CVGVAAILA 
        QPEPPFFRT WSSNPCLFM PFELDLKYN VFIIVLNVF 
        LASVSVIAF MATCFNHFK IKKHGFDYL KKSNSDLSD 
        LSLGNEQKN YYDTYTIQR GDHHLYDGQ NQVAWNSDV 
        SNISTTVSS TNSRRPCIT QQPVKNDEH RTGLETIHP 
        VLIVCYLFY HLPLIILCI YPGLVDPWD VAGVALWLG 
        LVQDVLIPT GLGIVDSRF CKWVSNVYK CSNNRGGDK 
        LPHVGLDGK FRPFSSPPQ SLEVAQNQR VMQTIEHRF 
        PITNGSLYT SIDGRLPVI HNYRRHKDQ RGVKTEFSH 
        VRPEQAYQR HNLEYSCRS CEADLCPSH SNLHHLSPQ 
        YINKQLNLI QNKNPLTKR TSLSQESLR YIPDTHFYQ 
        EQIKNSRNL LRLNKMRLC KSEDSLNDV TRELKFKAT 
        PDRVEYSSS DEEYLPENR NFDTLSSQS SYSITTEAN 
        CDFEFFQNN KERVVFENK VARPKIQRS SSYMTLEDR 
        NKKRNKKLL RSNSKLSDC THNIRNKVK SVEYLPNSN 
        ASTVFIEDV FYFNYPNQR KWGERNYVG SVPDLKKVF 
        ISEYI
      CDS sequence
      • >TC015091 RA
        atgttgtac gaaaccaag gcgcagttc gccgccgtc 
        gtggcgctg cccatcgtc accatcctc ctggtctgg 
        acctccgcc atcttccag cgcaaccgc aagtcttgg 
        ggcccttac gacatcccc atcgtcacc ctcctcatc 
        ctctccatc ctgcgcaac ctcaccgtc ctcacctac 
        accctcttc gtggccctg agccaggac gccatcaac 
        cacgactac tgcagcgcc ctcgtgtgg attttcaac 
        tcaattcac acgttccag gccagttct ttgacaacg 
        ctcgccgtc attggcctc ttctccgtc aaactccac 
        cgcaaacag caacatctg aggcagttc ctcaccgcc 
        acgcacgtc atctaccac ctgttctgc ctcacaacg 
        ctgtgcgca tgcgtcggc gtcgccgcc attttggcg 
        caacccgag ccgccattt tttagaact tggtcatcg 
        aatccgtgc ctgtttatg ccgtttgag ctcgatttg 
        aaatacaac gtgtttatt attgttttg aatgtgttt 
        ttggccagt gtgtcagtg atcgcgttc atggccacc 
        tgtttcaat catttcaaa atcaaaaaa cacggcttt 
        gactatttg aaaaagtcc aattcggat ctgagtgac 
        ttgagcctc ggcaatgaa caaaagaat tactacgat 
        acttatact atccaaaga ggtgatcac cacttatat 
        gatgggcag aatcaagtc gcttggaat agcgacgtg 
        tcgaatatt tcgacaact gtcagtagc accaacagc 
        agaaggccg tgcattacg caacagcct gtcaaaaac 
        gatgaacac agaactgga ttagaaact atacatccg 
        gttctaatc gtctgttat ctcttctat catctacct 
        ctcattata ctgtgtatt tatcccgga ttagtggac 
        ccgtgggac gtcgcaggt gtggcatta tggctaggc 
        ctggtccag gacgtctta atacccacg gggttagga 
        atcgttgat tcccgattt tgtaaatgg gtttcaaac 
        gtctacaag tgttccaac aatcgtggg ggcgacaaa 
        ttaccacac gttggcctt gacggtaag tttcggccg 
        tttagtagc ccacctcag tcactggaa gtggcccag 
        aatcagagg gtcatgcaa acaatcgag catcgattt 
        cccataacc aatggctct ctctacaca agtatagat 
        ggccgactt ccggtcatc cataattac agacgtcat 
        aaggaccaa agaggtgtc aaaactgag ttcagtcat 
        gtccggccg gagcaggcc taccaacgc cacaacttg 
        gaatattcc tgtcgcagt tgcgaggcc gatttgtgc 
        cccagccac tcaaacttg catcatttg agcccccaa 
        tacatcaac aaacaactg aacctaatc caaaataaa 
        aatccccta accaaacgc actagttta tcgcaggag 
        agtctccgg tacatcccc gatacgcac ttctaccag 
        gagcagata aaaaattcg cgtaatttg ctccgtttg 
        aataaaatg cggctgtgt aagagcgag gatagtctg 
        aatgacgtc actcgcgaa ctgaaattc aaagccacc 
        ccagatcgg gtggagtat tcgagctct gacgaggag 
        tatttgccc gaaaatcgc aactttgat actttgagt 
        tcgcaaagt tcgtactcg atcaccacc gaggccaac 
        tgcgacttt gagtttttc caaaataat aaggagagg 
        gttgttttc gagaataag gtggcaagg cctaagata 
        caaaggtcg agttcgtat atgactctg gaggatagg 
        aacaagaaa cggaataag aagttgttg cgttcgaat 
        tcgaaattg tccgattgt actcataat attaggaac 
        aaagtcaag tcagtcgag tatttgccg aattcgaac 
        gctagcact gtttttatc gaagacgtt ttctacttc 
        aattacccg aaccagcgt aaatggggc gaaaggaac 
        tacgtagga agtgtacct gatttgaaa aaagtcttt 
        atctcggag tatatttga
      mRNA sequence
      • >TC015091 RA
        cagagcggt tttgatcgc ggctggcga tgtttcacg 
        acaaaagaa aaaaaaatg tgaacggga agtgagtgg 
        attttagag tgccgtgga atttttcaa aaccgattt 
        ttttcgcgc caaggatac ctacttgat ggatttgga 
        gtaatattg cgtgaggcg tcgccgcca tgttgtacg 
        aaaccaagg cgcagttcg ccgccgtcg tggcgctgc 
        ccatcgtca ccatcctcc tggtctgga cctccgcca 
        tcttccagc gcaaccgca agtcttggg gcccttacg 
        acatcccca tcgtcaccc tcctcatcc tctccatcc 
        tgcgcaacc tcaccgtcc tcacctaca ccctcttcg 
        tggccctga gccaggacg ccatcaacc acgactact 
        gcagcgccc tcgtgtgga ttttcaact caattcaca 
        cgttccagg ccagttctt tgacaacgc tcgccgtca 
        ttggcctct tctccgtca aactccacc gcaaacagc 
        aacatctga ggcagttcc tcaccgcca cgcacgtca 
        tctaccacc tgttctgcc tcacaacgc tgtgcgcat 
        gcgtcggcg tcgccgcca ttttggcgc aacccgagc 
        cgccatttt ttagaactt ggtcatcga atccgtgcc 
        tgtttatgc cgtttgagc tcgatttga aatacaacg 
        tgtttatta ttgttttga atgtgtttt tggccagtg 
        tgtcagtga tcgcgttca tggccacct gtttcaatc 
        atttcaaaa tcaaaaaac acggctttg actatttga 
        aaaagtcca attcggatc tgagtgact tgagcctcg 
        gcaatgaac aaaagaatt actacgata cttatacta 
        tccaaagag gtgatcacc acttatatg atgggcaga 
        atcaagtcg cttggaata gcgacgtgt cgaatattt 
        cgacaactg tcagtagca ccaacagca gaaggccgt 
        gcattacgc aacagcctg tcaaaaacg atgaacaca 
        gaactggat tagaaacta tacatccgg ttctaatcg 
        tctgttatc tcttctatc atctacctc tcattatac 
        tgtgtattt atcccggat tagtggacc cgtgggacg 
        tcgcaggtg tggcattat ggctaggcc tggtccagg 
        acgtcttaa tacccacgg ggttaggaa tcgttgatt 
        cccgatttt gtaaatggg tttcaaacg tctacaagt 
        gttccaaca atcgtgggg gcgacaaat taccacacg 
        ttggccttg acggtaagt ttcggccgt ttagtagcc 
        cacctcagt cactggaag tggcccaga atcagaggg 
        tcatgcaaa caatcgagc atcgatttc ccataacca 
        atggctctc tctacacaa gtatagatg gccgacttc 
        cggtcatcc ataattaca gacgtcata aggaccaaa 
        gaggtgtca aaactgagt tcagtcatg tccggccgg 
        agcaggcct accaacgcc acaacttgg aatattcct 
        gtcgcagtt gcgaggccg atttgtgcc ccagccact 
        caaacttgc atcatttga gcccccaat acatcaaca 
        aacaactga acctaatcc aaaataaaa atcccctaa 
        ccaaacgca ctagtttat cgcaggaga gtctccggt 
        acatccccg atacgcact tctaccagg agcagataa 
        aaaattcgc gtaatttgc tccgtttga ataaaatgc 
        ggctgtgta agagcgagg atagtctga atgacgtca 
        ctcgcgaac tgaaattca aagccaccc cagatcggg 
        tggagtatt cgagctctg acgaggagt atttgcccg 
        aaaatcgca actttgata ctttgagtt cgcaaagtt 
        cgtactcga tcaccaccg aggccaact gcgactttg 
        agtttttcc aaaataata aggagaggg ttgttttcg 
        agaataagg tggcaaggc ctaagatac aaaggtcga 
        gttcgtata tgactctgg aggatagga acaagaaac 
        ggaataaga agttgttgc gttcgaatt cgaaattgt 
        ccgattgta ctcataata ttaggaaca aagtcaagt 
        cagtcgagt atttgccga attcgaacg ctagcactg 
        tttttatcg aagacgttt tctacttca attacccga 
        accagcgta aatggggcg aaaggaact acgtaggaa 
        gtgtacctg atttgaaaa aagtcttta tctcggagt 
        atatttgaa ctgtatata ttgtactgt acatatttt 
        aat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY