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Gene information
General information
TC015082 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3576071..3594324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015082
    iBeetle screen iB_02397

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015082 RA
      MSRNIRKHD TGPKHLSFD SVVFSMFTK EEIKKLCIT 
      KICTPLTLD PLGHPLSGG LYDKSLGPM TEKSEPCGT 
      CRKNLYFCP GHFGYIELP LPVVNPIFY RMIGTILRM 
      ACLSCFRFQ IPEHVKYSI TLQIRLLNC GLVTEAVEI 
      ANSIALLIL VHESLENIP EDVVLPIRK YETLAQEMQ 
      EKLNGEYVL TKNTETLRN QFVNEMLRE VKARETCMH 
      CKHSMEKVQ VLRNKIILS KKRNVAKGS TEVLGKVKA 
      FEAKYVTPD ESRTYMRNI WKEEKELMR EIIAVLGGV 
      EGEHPTDVF YWDVIPVIP PNMRPVNIV NSRVSEHPT 
      SQLYKNIIH NTILLRLII QVVKNKGDL GSLPPEAKG 
      AYNVSKGSS PIEKLNLAW ESLQSEVDS LIDTTYIRN 
      ANVAGLKQI IEKKTGIIR MHMMGKRVN FSARTVITP 
      DPNLNIDEI GIPEVFAKH LTYPVPVTS WNVEELRKM 
      IMNGPDVHP GAVMVEYED GTIKRINTN NTTQQESIL 
      KRLLTPDKI VGFRGVKRV HRHLCNGDV LLLNRQPTL 
      HKPSIMAHT ARVLKGEKT FRLHYANCK AYNADFDGD 
      EMNAHFPQN ELARSEAYN LVNVCNQYL VPKDGTPLS 
      GLIQDHMIA GVKLSLRGR FFNEVDYKH FVFQALANK 
      TGKIILLPP AIIKPCQLW SGKQILSTV FINIIPEGH 
      ERINLTASA KISAKAWQT APPRKWKGG GSRFVNPNT 
      MSEAEVVIR GGELLVGVL DKTHYGATP YGLVHCMYE 
      LYGGVYATG LLSALAKLF TRFLQKEGF TLGVHDILT 
      VEKADQRRR QIIEGARKI GLDVITTAL DMPRDSPIE 
      EIVDKIREN SVKNPKWRA TVDRQYKTA LDSFTNDIN 
      KTCLPAGLV LKFPENNLQ LMVQSGAKG TTVNTMQIS 
      CLLGQIELE GKRPPVMIS GKSLPSFPI FEFAPRAGG 
      FIDGRFMTG IQPQEFFFH CMAGREGLI DTAVKTSRS 
      GYLQRCLIK HLEGLTVGY DLTVRDSDK CVIQFLYGE 
      DGMDISKLQ FLNSKQLVF LADNSKGMI DKKALKMLK 
      DEHQKSVKA HKQQLADWC EKFGHPLQR ERKSPFSLF 
      SSVVRAKTK GTDFGTKEM TKLWKKADK DIKDSFRQQ 
      CIPCPDPVN SIYQPDHHF GSLNEKLEE LMNTYQSTN 
      KKQTKQFQN MLKLKAMRS LCAPGEPVG LLAAQSIGE 
      PSTQMTLNT FHFAGRGEM NVTLGIPRL REILMMASK 
      NIKTPSMEI PFLPVENLE EKAEDLRKM LTRVVVADV 
      LEKVDVTVE LNVEPVRQH RYTLRFQFL PRKYYKNDF 
      YVGPKYILK HMTRKFFGE MFTAIRKLS KVTSNLVMM 
      DEEKRQKAS NDDEEETND ADANEDSSD EEPEDTEDA 
      KTGNKFKQI HDDQEPEES EREDSGDED TETAPPVDD 
      KTNEAVVES YNFAQNYIY DTKKHHWCE ITFGLPLEF 
      KKLDLTAIL RDVAAKSVL WETPNIKRA ITYMKNDVL 
      TLRTDGINI LEMSKYHAL LDLNRLNCN DIHKMAETY 
      GIEAAMKII VKEVQDVFN VYGIKVDPR HLSLIADYM 
      TFNGTFEPL SRKGMESSA SPLQQMTFE SSLTFLRNA 
      TIGGKRDNL TNPSSCLMV GKPCVTGTG CFEVLHKM
    CDS sequence
    • >TC015082 RA
      atgtcccga aacatccgc aagcacgac acgggcccc 
      aaacacctg tctttcgac tcggtcgtg ttctccatg 
      ttcacgaaa gaagagatc aaaaaattg tgcattacg 
      aaaatatgc acccccttg actttggac cccttgggg 
      cacccgttg tccggcggc ctctacgac aagtctctg 
      ggccccatg accgagaag agcgagccg tgcggcact 
      tgccgcaaa aacctttac ttttgcccg gggcatttc 
      gggtacatt gagctgccc ctgcctgtg gttaacccc 
      attttttac cgcatgatt gggactatt ctgcggatg 
      gcttgcctg agttgtttc cggtttcaa attcccgag 
      catgtgaaa tattcaatt acgctgcaa attcggctt 
      ttgaattgc gggctggtc acggaggct gtggaaatt 
      gccaatagc atagcactg ttgattttg gtgcacgag 
      tcgttggag aatattcct gaagatgtg gttttgccg 
      attcgaaag tacgaaacg ttggctcag gagatgcaa 
      gaaaaattg aacggggaa tacgttcta acgaaaaat 
      actgagact ttacgcaat cagtttgtg aacgagatg 
      ttgcgcgag gtgaaggcg agggagact tgtatgcac 
      tgtaaacat tcaatggaa aaagtgcaa gttttgaga 
      aataagatt attctctcg aagaagcga aacgttgcc 
      aaggggagt acggaagtt ttggggaaa gttaaggct 
      tttgaggcg aaatatgtg actcctgat gagtcacgg 
      acttatatg agaaacata tggaaggag gagaaggaa 
      ttgatgagg gaaattatc gccgtttta gggggggtg 
      gagggagag caccctact gatgttttt tattgggat 
      gttattccg gtgattccc ccaaatatg agacccgtc 
      aatattgtt aatagtcgt gttagtgaa catccgact 
      tcgcaattg tataaaaat attattcat aatacaatt 
      cttctgaga ttgattatt caagtggtg aagaataag 
      ggggacttg ggtagtctt ccacccgaa gctaagggc 
      gcctacaac gtatccaag ggcagctct cccatcgaa 
      aaactgaac ctagcctgg gaatccctc caatccgag 
      gtcgactcc ctcatcgac accacctac atccgcaat 
      gcaaacgtc gccggtttg aaacaaatc atcgaaaag 
      aaaaccgga atcattcgc atgcacatg atgggcaaa 
      cgtgtcaat ttctccgcc cgtaccgtc atcacaccc 
      gaccccaac ctcaacatt gacgaaatc ggcattccg 
      gaagttttt gccaaacat ctgacgtat cccgtaccc 
      gtgacgtca tggaacgtc gaagagttg cgcaaaatg 
      ataatgaac ggaccggac gtacatccg ggcgctgtc 
      atggtcgaa tacgaagac ggaacaata aagcgtata 
      aacacaaac aacacaacc caacaagaa agcattctg 
      aaacggctt ctaacgccc gataaaatc gtgggcttc 
      cgtggcgtc aaacgcgtg catagacac ctatgcaac 
      ggcgatgtg cttttgttg aatcgacag cctactttg 
      cacaagccg agcataatg gcacacacg gcccgtgtt 
      ttaaaaggg gagaaaacg ttcaggtta cattacgcc 
      aactgtaag gcgtacaac gccgacttt gatggcgac 
      gaaatgaac gcacatttc ccccaaaat gagttagca 
      cgtagcgaa gcttacaac ttagttaac gtttgtaac 
      caatatctc gtaccaaaa gatggcact cctttaagt 
      ggccttatc caagatcac atgatcgca ggagtgaaa 
      ttatcactc cgggggcga tttttcaac gaagtcgat 
      tacaaacat ttcgttttc caagctttg gcaaacaaa 
      actggaaaa atcatccta ttaccaccg gcaatcata 
      aaaccgtgc caactatgg tccggtaaa caaatcctc 
      tcgactgtt ttcattaac ataatcccg gaaggccac 
      gaacgcatt aacttgaca gcgagtgcg aaaatcagc 
      gctaaggcg tggcagacg gccccgccc cgcaaatgg 
      aaagggggc ggttcccgt tttgtgaac cccaatacg 
      atgagcgag gccgaggtt gtgattcgc ggcggcgag 
      ttgttggtg ggcgtgttg gacaagacg cattatggg 
      gccacgccc tatgggctt gtccattgc atgtacgag 
      ttgtacggg ggcgtgtat gcgacaggg ctgttgagc 
      gcattggct aaactcttc acgaggttt ctgcaaaag 
      gaggggttt actttgggc gtgcatgat atcttgacg 
      gtggagaag gcggaccag aggcggcgc caaatcatc 
      gaaggggcg cggaaaatc ggccttgac gtgatcacg 
      accgcgctc gacatgccc cgagactcc ccgatcgag 
      gaaatcgtc gacaaaatc cgagaaaat agcgtcaaa 
      aaccccaaa tggagggca acggttgac cgacaatac 
      aaaacggca ctagattca ttcacgaat gatattaac 
      aagacttgt ttaccggct ggtttagtg ttaaaattt 
      cctgaaaat aatctccag ttgatggta caatcgggc 
      gctaaaggc acaacagtc aataccatg caaatttcg 
      tgtcttttg ggccaaatt gagttggag ggtaaacgc 
      ccccctgtc atgataagc gggaaatcg ttgcccagt 
      tttccaatt tttgagttt gcgccacgt gctggtggt 
      tttatcgat ggcaggttt atgactggg atccaaccc 
      caagagttt ttcttccat tgtatggca ggtcgtgag 
      gggttgatt gatacagct gttaaaact agtcgtagt 
      gggtattta cagaggtgt ttgattaaa catttggaa 
      ggtttgacg gttggctat gatttgaca gttcgtgat 
      agtgacaag tgtgtcatc cagtttctg tatggcgaa 
      gatggaatg gatatttca aaattgcaa tttcttaat 
      tcgaaacag ttggtcttt ttggccgat aacagtaaa 
      ggtatgatt gataagaag gcactgaag atgttgaaa 
      gatgaacat cagaagtcg gttaaagct cacaaacaa 
      caattggcc gattggtgt gaaaaattc ggacacccg 
      ttacagaga gaacgaaaa agtccgttt tctttgttt 
      tcgtctgtg gtgcgtgct aagaccaaa ggaacagat 
      tttggtacg aaggaaatg accaagttg tggaaaaag 
      gccgacaaa gacataaaa gatagcttc cgtcagcag 
      tgcatcccc tgccccgac cccgtcaac tccatctac 
      caaccggac caccacttc ggttccctc aacgaaaaa 
      ctcgaagaa ttgatgaac acttaccaa tccaccaac 
      aaaaaacaa accaaacaa ttccaaaac atgctaaaa 
      ctcaaagct atgcgttca ctgtgcgct cccggcgaa 
      cctgtcggc ctcctcgca gcgcaatct atcggcgaa 
      ccttccact cagatgacc ctcaacacg tttcatttc 
      gccggcaga ggcgaaatg aacgtcacc ttaggtata 
      ccacgtctg agggaaatc ctgatgatg gcgtcgaaa 
      aacatcaaa acgccctca atggaaatc cccttcctg 
      cctgtggaa aacctggag gagaaggcg gaggatttg 
      aggaaaatg ttgacaagg gtcgtagtt gccgacgtt 
      ttggaaaaa gttgatgtc acagttgaa ttaaatgtt 
      gagccggtc aggcaacat aggtacact ctgaggttc 
      cagtttttg ccccggaag tactacaag aacgatttt 
      tacgtcgga ccgaagtat attttgaag cacatgacg 
      cgcaaattc ttcggcgaa atgttcaca gcgattagg 
      aaactgtcc aaagtgacc tcaaatttg gtaatgatg 
      gacgaggaa aagcgtcaa aaagcgtca aacgacgac 
      gaggaggaa acaaacgac gcagacgcg aacgaagac 
      agttccgac gaggagccg gaagacacc gaagacgcg 
      aaaacgggc aacaagttc aagcaaatc cacgacgac 
      caagagccg gaagagtcc gaacgcgaa gactccggg 
      gacgaggac accgaaacg gcgccccct gtggacgac 
      aaaacgaac gaagcggtg gttgaaagc tacaatttc 
      gcgcaaaac tacatctac gacacgaaa aaacaccac 
      tggtgcgaa atcacattc ggacttccg ctcgaattc 
      aaaaaactc gacttgacc gccatcttg cgtgacgtc 
      gccgcgaag tccgtgttg tgggaaacc cccaatatc 
      aagagggct attacctac atgaagaat gatgttttg 
      accttacgg accgacggt attaatatt ttggaaatg 
      tcgaagtac cacgccctc ttggatttg aaccgtttg 
      aattgtaat gatatacac aagatggcg gagacttat 
      gggattgaa gccgccatg aaaattata gtgaaggag 
      gttcaggac gtgtttaat gtttatggg attaaggtc 
      gatccgcgc catctgtcg ttaattgcc gattatatg 
      acgtttaat ggcacgttt gaaccgttg agtcgtaaa 
      gggatggaa agtagtgcc tcaccgctc caacagatg 
      acgttcgag tcgtcgctc actttccta cgtaatgct 
      acgattggt gggaaaagg gacaatttg accaatccg 
      tccagttgt ttgatggtt ggaaaacca tgtgtgaca 
      gggacgggt tgttttgag gtgttgcac aaaatgtga
    mRNA sequence
    • >TC015082 RA
      gataaaaac gtcatcttt aatgccatt ttcattaca 
      atactgatt gaataattt tgattcttt ttttattgc 
      aagattttt aattttttt ttcatattt cttgtgtaa 
      gtttttttc ttcttttct gcagtaatt agtgaattg 
      aataaattt gaaactatt tggctcggc gatattttt 
      tgtcctccg tagttcgtt ttaatgtga tgtaacctc 
      gttgccata caagttttt ttccaagga tttaaacat 
      tccactgag attttaatt cgcgactga tttgattgt 
      acatatttc aaataatgt aaatatttc attaatcgc 
      acaatgtaa ataaatata ctgtaacag ttgagttgt 
      tgttagtgc aatattctt gaagtttct cgcaaaatt 
      ctgtttcag tgacttaac ccaataatc aatcgcatt 
      gaaattgta tatttttat aaataatgt aaatagttt 
      tacttgaac attcctatt aggtaggga aaagatttt 
      caatctttg tcaatatta ttaatattc acttagcac 
      aattttaat atgctattt ctagtgttt aagatttct 
      acttgactt aaaaaatga attgatata ataatgata 
      ccaagcttt cgcgtttcg tttcaatta ggtgtttct 
      taggacttt agagcaact tttcgatac agaagtagc 
      cacactgta tagcaattt tacgttgct ctaaacgcg 
      tattcttta tttctctgt ttttttaaa aaataccac 
      acgaaaatt gttgtaata ctgtaattc tcattgtgt 
      ttcgtctta tctggcacc tacgtaata aacaaggtt 
      gtaaaattt agtattttc tgtcaattc aaatctgtg 
      atgtcaata aataaatga ctggaacaa ctacacact 
      tttctgctt cctcaacac cgcaaccat tatcttcat 
      ccagaactc cagtattta aaatactgt tccccctct 
      aacgagccg ccactgcgt tttaaaatc ggttcggga 
      agtagaggc caaatacaa tgagaaata ccacactag 
      aaaaggaca gcgactggg gtgccgtta gtaacgtag 
      agcgctatc cttttctac cgtacgtct agtccagat 
      tgagcaaac tctacacaa gtacacagt cacgtggtt 
      ttagtaaca gttccgtgc agtgcaacc aacataagc 
      ctaaaccaa gtttttttt gaaaatttt tcgcatttt 
      cgccgtttt tcgaacgca aaaactagc caaaacaat 
      tgtaaatga aacgcccag tgtttttac aatgctttt 
      caattacta ttaagtgat aattgccac actttgatt 
      ttgcggtta tgtgcaagt gaaaaacgt gtctcaaca 
      tgtcccgaa acatccgca agcacgaca cgggcccca 
      aacacctgt ctttcgact cggtcgtgt tctccatgt 
      tcacgaaag aagagatca aaaaattgt gcattacga 
      aaatatgca cccccttga ctttggacc ccttggggc 
      acccgttgt ccggcggcc tctacgaca agtctctgg 
      gccccatga ccgagaaga gcgagccgt gcggcactt 
      gccgcaaaa acctttact tttgcccgg ggcatttcg 
      ggtacattg agctgcccc tgcctgtgg ttaacccca 
      ttttttacc gcatgattg ggactattc tgcggatgg 
      cttgcctga gttgtttcc ggtttcaaa ttcccgagc 
      atgtgaaat attcaatta cgctgcaaa ttcggcttt 
      tgaattgcg ggctggtca cggaggctg tggaaattg 
      ccaatagca tagcactgt tgattttgg tgcacgagt 
      cgttggaga atattcctg aagatgtgg ttttgccga 
      ttcgaaagt acgaaacgt tggctcagg agatgcaag 
      aaaaattga acggggaat acgttctaa cgaaaaata 
      ctgagactt tacgcaatc agtttgtga acgagatgt 
      tgcgcgagg tgaaggcga gggagactt gtatgcact 
      gtaaacatt caatggaaa aagtgcaag ttttgagaa 
      ataagatta ttctctcga agaagcgaa acgttgcca 
      aggggagta cggaagttt tggggaaag ttaaggctt 
      ttgaggcga aatatgtga ctcctgatg agtcacgga 
      cttatatga gaaacatat ggaaggagg agaaggaat 
      tgatgaggg aaattatcg ccgttttag ggggggtgg 
      agggagagc accctactg atgtttttt attgggatg 
      ttattccgg tgattcccc caaatatga gacccgtca 
      atattgtta atagtcgtg ttagtgaac atccgactt 
      cgcaattgt ataaaaata ttattcata atacaattc 
      ttctgagat tgattattc aagtggtga agaataagg 
      gggacttgg gtagtcttc cacccgaag ctaagggcg 
      cctacaacg tatccaagg gcagctctc ccatcgaaa 
      aactgaacc tagcctggg aatccctcc aatccgagg 
      tcgactccc tcatcgaca ccacctaca tccgcaatg 
      caaacgtcg ccggtttga aacaaatca tcgaaaaga 
      aaaccggaa tcattcgca tgcacatga tgggcaaac 
      gtgtcaatt tctccgccc gtaccgtca tcacacccg 
      accccaacc tcaacattg acgaaatcg gcattccgg 
      aagtttttg ccaaacatc tgacgtatc ccgtacccg 
      tgacgtcat ggaacgtcg aagagttgc gcaaaatga 
      taatgaacg gaccggacg tacatccgg gcgctgtca 
      tggtcgaat acgaagacg gaacaataa agcgtataa 
      acacaaaca acacaaccc aacaagaaa gcattctga 
      aacggcttc taacgcccg ataaaatcg tgggcttcc 
      gtggcgtca aacgcgtgc atagacacc tatgcaacg 
      gcgatgtgc ttttgttga atcgacagc ctactttgc 
      acaagccga gcataatgg cacacacgg cccgtgttt 
      taaaagggg agaaaacgt tcaggttac attacgcca 
      actgtaagg cgtacaacg ccgactttg atggcgacg 
      aaatgaacg cacatttcc cccaaaatg agttagcac 
      gtagcgaag cttacaact tagttaacg tttgtaacc 
      aatatctcg taccaaaag atggcactc ctttaagtg 
      gccttatcc aagatcaca tgatcgcag gagtgaaat 
      tatcactcc gggggcgat ttttcaacg aagtcgatt 
      acaaacatt tcgttttcc aagctttgg caaacaaaa 
      ctggaaaaa tcatcctat taccaccgg caatcataa 
      aaccgtgcc aactatggt ccggtaaac aaatcctct 
      cgactgttt tcattaaca taatcccgg aaggccacg 
      aacgcatta acttgacag cgagtgcga aaatcagcg 
      ctaaggcgt ggcagacgg ccccgcccc gcaaatgga 
      aagggggcg gttcccgtt ttgtgaacc ccaatacga 
      tgagcgagg ccgaggttg tgattcgcg gcggcgagt 
      tgttggtgg gcgtgttgg acaagacgc attatgggg 
      ccacgccct atgggcttg tccattgca tgtacgagt 
      tgtacgggg gcgtgtatg cgacagggc tgttgagcg 
      cattggcta aactcttca cgaggtttc tgcaaaagg 
      aggggttta ctttgggcg tgcatgata tcttgacgg 
      tggagaagg cggaccaga ggcggcgcc aaatcatcg 
      aaggggcgc ggaaaatcg gccttgacg tgatcacga 
      ccgcgctcg acatgcccc gagactccc cgatcgagg 
      aaatcgtcg acaaaatcc gagaaaata gcgtcaaaa 
      accccaaat ggagggcaa cggttgacc gacaataca 
      aaacggcac tagattcat tcacgaatg atattaaca 
      agacttgtt taccggctg gtttagtgt taaaatttc 
      ctgaaaata atctccagt tgatggtac aatcgggcg 
      ctaaaggca caacagtca ataccatgc aaatttcgt 
      gtcttttgg gccaaattg agttggagg gtaaacgcc 
      cccctgtca tgataagcg ggaaatcgt tgcccagtt 
      ttccaattt ttgagtttg cgccacgtg ctggtggtt 
      ttatcgatg gcaggttta tgactggga tccaacccc 
      aagagtttt tcttccatt gtatggcag gtcgtgagg 
      ggttgattg atacagctg ttaaaacta gtcgtagtg 
      ggtatttac agaggtgtt tgattaaac atttggaag 
      gtttgacgg ttggctatg atttgacag ttcgtgata 
      gtgacaagt gtgtcatcc agtttctgt atggcgaag 
      atggaatgg atatttcaa aattgcaat ttcttaatt 
      cgaaacagt tggtctttt tggccgata acagtaaag 
      gtatgattg ataagaagg cactgaaga tgttgaaag 
      atgaacatc agaagtcgg ttaaagctc acaaacaac 
      aattggccg attggtgtg aaaaattcg gacacccgt 
      tacagagag aacgaaaaa gtccgtttt ctttgtttt 
      cgtctgtgg tgcgtgcta agaccaaag gaacagatt 
      ttggtacga aggaaatga ccaagttgt ggaaaaagg 
      ccgacaaag acataaaag atagcttcc gtcagcagt 
      gcatcccct gccccgacc ccgtcaact ccatctacc 
      aaccggacc accacttcg gttccctca acgaaaaac 
      tcgaagaat tgatgaaca cttaccaat ccaccaaca 
      aaaaacaaa ccaaacaat tccaaaaca tgctaaaac 
      tcaaagcta tgcgttcac tgtgcgctc ccggcgaac 
      ctgtcggcc tcctcgcag cgcaatcta tcggcgaac 
      cttccactc agatgaccc tcaacacgt ttcatttcg 
      ccggcagag gcgaaatga acgtcacct taggtatac 
      cacgtctga gggaaatcc tgatgatgg cgtcgaaaa 
      acatcaaaa cgccctcaa tggaaatcc ccttcctgc 
      ctgtggaaa acctggagg agaaggcgg aggatttga 
      ggaaaatgt tgacaaggg tcgtagttg ccgacgttt 
      tggaaaaag ttgatgtca cagttgaat taaatgttg 
      agccggtca ggcaacata ggtacactc tgaggttcc 
      agtttttgc cccggaagt actacaaga acgattttt 
      acgtcggac cgaagtata ttttgaagc acatgacgc 
      gcaaattct tcggcgaaa tgttcacag cgattagga 
      aactgtcca aagtgacct caaatttgg taatgatgg 
      acgaggaaa agcgtcaaa aagcgtcaa acgacgacg 
      aggaggaaa caaacgacg cagacgcga acgaagaca 
      gttccgacg aggagccgg aagacaccg aagacgcga 
      aaacgggca acaagttca agcaaatcc acgacgacc 
      aagagccgg aagagtccg aacgcgaag actccgggg 
      acgaggaca ccgaaacgg cgccccctg tggacgaca 
      aaacgaacg aagcggtgg ttgaaagct acaatttcg 
      cgcaaaact acatctacg acacgaaaa aacaccact 
      ggtgcgaaa tcacattcg gacttccgc tcgaattca 
      aaaaactcg acttgaccg ccatcttgc gtgacgtcg 
      ccgcgaagt ccgtgttgt gggaaaccc ccaatatca 
      agagggcta ttacctaca tgaagaatg atgttttga 
      ccttacgga ccgacggta ttaatattt tggaaatgt 
      cgaagtacc acgccctct tggatttga accgtttga 
      attgtaatg atatacaca agatggcgg agacttatg 
      ggattgaag ccgccatga aaattatag tgaaggagg 
      ttcaggacg tgtttaatg tttatggga ttaaggtcg 
      atccgcgcc atctgtcgt taattgccg attatatga 
      cgtttaatg gcacgtttg aaccgttga gtcgtaaag 
      ggatggaaa gtagtgcct caccgctcc aacagatga 
      cgttcgagt cgtcgctca ctttcctac gtaatgcta 
      cgattggtg ggaaaaggg acaatttga ccaatccgt 
      ccagttgtt tgatggttg gaaaaccat gtgtgacag 
      ggacgggtt gttttgagg tgttgcaca aaatgtgag 
      gttttgttt aaataaagt tttgttttt att
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