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Gene information
General information
TC015080 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid40: 2689380..2690650
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015080
    iBeetle screen iB_02396
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva anterior partiallydecreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015080 RA
      MEEFPKLVN SHDDSVITI NNSSFSIGR SLSCDHIIE 
      SLGVSRFHC FIKKNGDRW TLFDRSTNG TIVNSTLYK 
      FNQSTELKD GDVIKLGPD NLDFVFTCS HPPKSSESH 
      ATPLNEQDF NAIERELEV IDEALLQEL EEPFAAEGA 
      PPEAKPQPE PNSEDLDTE LTCSICSEL FIKAVTLNC 
      SHTFCKFCI DRWMKNKSN CPICRKSIT NIAPTLVLD 
      NFIEKFIKT QSDDVKETR KNLIQQREE MVVNMASSS 
      TRSPEVVDV EPGRVIEIY SDDEDYLDV EDEDSDYNY 
      LSDDHDYDV YDCYPDRPR GRYYGGYGR CFICNQAGH 
      WANSCPFKR L
    CDS sequence
    • >TC015080 RA
      atggaagaa tttccgaaa ttagtgaat tcgcacgat 
      gattccgtc attacaatc aacaattca tccttctca 
      atcgggcga agcctcagc tgtgatcac atcattgag 
      tccctcggt gtttcccga tttcactgc ttcattaag 
      aagaacggc gaccggtgg acgctcttc gaccggagc 
      acgaatgga acaatcgtc aactccaca ctttacaaa 
      ttcaaccaa agcaccgaa ctgaaggac ggggacgtg 
      atcaagtta gggcccgac aatttagac ttcgtcttc 
      acttgttca cacccgcct aaaagtagt gaatctcac 
      gccacgcct ttaaacgag caagatttc aacgcaatt 
      gagcgagag ttggaggtg atagacgag gcgcttttg 
      caggagttg gaggagccc tttgcggcc gagggcgcg 
      cctccggag gccaagccc cagccggag cccaactcg 
      gaggatttg gacacagag ctcacttgt agcatctgc 
      tccgagctg ttcatcaag gctgttaca ctgaactgt 
      tcgcacacg ttttgcaag ttttgcatc gataggtgg 
      atgaagaac aagtcgaat tgtcccatt tgccgcaaa 
      tctatcaca aatatcgcg ccgactcta gtccttgat 
      aattttatc gagaagttt attaaaacg caaagtgat 
      gacgtgaaa gagacgcgg aaaaatcta atccagcag 
      agggaggag atggttgtt aatatggcg agtagttcg 
      actaggtct ccagaggtg gtggacgtg gagccaggg 
      cgtgtgatt gagatttat tccgatgat gaggattat 
      ctggatgtg gaagatgag gatagcgat tacaattat 
      ttaagtgat gaccatgac tatgatgtg tatgattgt 
      tacccggat aggcccagg gggaggtat tatgggggc 
      tatgggagg tgctttatt tgcaaccag gccgggcat 
      tgggccaac agttgcccc ttcaagagg ctatga
    mRNA sequence
    • >TC015080 RA
      tgcgcagca gtcacttgg tggggaaca aaactccct 
      aaatttttc ttgtttgtt ggtcggatg tcagttaca 
      aatcaaaat aatggaaga atttccgaa attagtgaa 
      ttcgcacga tgattccgt cattacaat caacaattc 
      atccttctc aatcgggcg aagcctcag ctgtgatca 
      catcattga gtccctcgg tgtttcccg atttcactg 
      cttcattaa gaagaacgg cgaccggtg gacgctctt 
      cgaccggag cacgaatgg aacaatcgt caactccac 
      actttacaa attcaacca aagcaccga actgaagga 
      cggggacgt gatcaagtt agggcccga caatttaga 
      cttcgtctt cacttgttc acacccgcc taaaagtag 
      tgaatctca cgccacgcc tttaaacga gcaagattt 
      caacgcaat tgagcgaga gttggaggt gatagacga 
      ggcgctttt gcaggagtt ggaggagcc ctttgcggc 
      cgagggcgc gcctccgga ggccaagcc ccagccgga 
      gcccaactc ggaggattt ggacacaga gctcacttg 
      tagcatctg ctccgagct gttcatcaa ggctgttac 
      actgaactg ttcgcacac gttttgcaa gttttgcat 
      cgataggtg gatgaagaa caagtcgaa ttgtcccat 
      ttgccgcaa atctatcac aaatatcgc gccgactct 
      agtccttga taattttat cgagaagtt tattaaaac 
      gcaaagtga tgacgtgaa agagacgcg gaaaaatct 
      aatccagca gagggagga gatggttgt taatatggc 
      gagtagttc gactaggtc tccagaggt ggtggacgt 
      ggagccagg gcgtgtgat tgagattta ttccgatga 
      tgaggatta tctggatgt ggaagatga ggatagcga 
      ttacaatta tttaagtga tgaccatga ctatgatgt 
      gtatgattg ttacccgga taggcccag ggggaggta 
      ttatggggg ctatgggag gtgctttat ttgcaacca 
      ggccgggca ttgggccaa cagttgccc cttcaagag 
      gctatgaaa tgtgtaatt ttggtagtt gtctaatta 
      aatcataaa atgtttgtt ttttttcta ttatcttgc 
      taaatacaa ttatgtttt tttg