| General information | |||
| TC033410 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 3681587..3703204 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033410 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02394 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC033410 RA
MGAKDSKRS CLPYEDAVK RMSEEEYKR ICEAFKRLS GGNQLLSKQ SFILHVLGD GVPQAIAEW LYLACGGTP RGIALKDLV CGLVLLTKG KQDEKIRFL FTLYCNDSG THITRSEFY SALQTEGTL PPDKPNRPN ILWERTMLS LFPVGQDRV TYEHFSAWI QYNKEATIL SKWLLSNPC VSLSSEVEA PTFYQTLAG VTHLEEQDI CELEKCFWT LQGSSPSCH LDLNCVRSM VCPPIPLTA CEGLFLALD VNRDGHIDF KELCCGISA ACRGPIAER MKFCFKIFD MDRDCYLNP DELQHMIHT LMLVANENN KRFNGYDGN FDSDKEEDH SKILQSLTQ RLDERGGLS QEEFLVWGV EDNGLVEPM LELLFQVCH VSFVLRPAC RHHEHDIVT GWLEREERR GYHVGQFWY LISSEWWQN WLSYTTAPR NNLDHCTCR TATDPRLVE EGIVCDESL ISNGTDSNE FNSNSMESM GDLLGKGDT CSIASSSGV SSSSGTTGS KRSQGMPGP IDNSNLVAE PLHKVRTLT GEGGRLKRD TPLVQHRDF ELVPDSLWK ALAHWYGGP LPLPRQVIL PPGSCDVEL ELYPLNLSI WMHQSQAVG SAASWSSVV GGYGAAALN TAGITTSPI SSPRRYLAH TAAFSRLAN VRQVIEFLC LRLSLRVED VRLWHIRDT TTLLEDENA TLQDLSVID GDKILLEVR NKDLTWPEE LGALVAGGQ TTLSAERRP TICLPPGAT GLHNLGNTC FMNAALQAV SNTRPLTLY FQRDGQLCE LNSVNPLGT KGMVARRYA ELCRELWAG STRSVAPLK LRYCVTKHA PHLGGGGQH DSQELLAWL LDALHEDLN RVARKQYSE LKDSGGRPD EVVAEEAWV QHLARDHSI ITDLFYGQL KSKVTCQTC GHESVRFDP FNLLSLPLP MESYTLCEV LVVRLNGET PIKYGLRLN SEAKYIELK QQLERLCNI KTERLLLAE LAYSQIRQV LNDDVRINP GTATELYAY ELPEDHQET EEISNDVDV EVAVSDSPL ARSPENRQK TDSHLMNGG VVLKKASII SHSFSDTSS TSSKFPSYL IALHRKCVR QETYFLSQQ KSKPSLFGS PLLLGFRPN STCQSLYEA VWGQVTRFL SPLPQSDQT NHATDCDDS LGYEFPFTL KAVLQGGQI CAICHWTQF CRGCTLPCS NDLVVDHCR GNLNGMCIA IDWDPTALH LRYQSNREK LWIEDESVS RCQKLHTEP IDLDNCLKA FTSEERLET KYHCSNCQD KQPATKKLQ IWRLPPILI IHLKRFDCV NNKWVKTQK VVNFPFKDF DPTAYLASV PQETILRHL QLREKRQDL ESSCDSQTS VKTIEESTE TNCTTNEHS KLLTTDNKP KVQLNKVRA RLESTSLLK TPIIDEHLK DYHQHKLLP GQDPFDLKY QLYAVVSHS GMLNGGHYI SYACNPNGN WYCYNDSSC REVLTEIVE PVKPKKSTC TPLRRRKNT QSSEKEEET CNTDPKETT CDDTTVTKP EPNYKKCAY SDVKTPKID TSSAYILFY ERSGLDYKP YLPEVVSNG QIVPEVELD ENESELRKQ LCSIQ
- >TC033410 RB
MGAKDSKRS CLPYEDAVK RMSEEEYKR ICEAFKRLS GGNQLLSKQ SFILHVLGD GVPQAIAEW LYLACGGTP RGIALKDLV CGLVLLTKG KQDEKIRFL FTLYCNDSG THITRSEFY SALQTEGTL PPDKPNRPN ILWERTMLS LFPVGQDRV TYEHFSAWI QYNKEATIL SKWLLSNPC VSLSSEVEA PTFYQTLAG VTHLEEQDI CELEKCFWT LQGSSPSCH LDLNCVRSM VCPPIPLTA CEGLFLALD VNRDGHIDF KELCCGISA ACRGPIAER MKFCFKIFD MDRDCYLNP DELQHMIHT LMLVANENN KRFNGYDGN FDSDKEEDH SKILQSLTQ RLDERGGLS QEEFLVWGV EDNGLVEPM LELLFQVCH VSFVLRPAC RHHEHDIVT GWLEREERR GYHVGQFWY LISSEWWQN WLSYTTAPR NNLDHCTCR TATDPRLVE EGIVCDESL ISNGTDSNE FNSNSMESM GDLLGKGDT CSIASSSGV SSSSGTTGS KRSQGMPGP IDNSNLVAE PLHKVRTLT GEGGRLKRD TPLVQHRDF ELVPDSLWK ALAHWYGGP LPLPRQVIL PPGSCDVEL ELYPLNLSI WMHQSQAVG SAASWSSVV GGYGAAALN TAGITTSPI SSPRRYLAH TAAFSRLAN VRQVIEFLC LRLSLRVED VRLWHIRDT TTLLEDENA TLQDLSVID GDKILLEVR NKDLTWPEE LGALVAGGQ TTLSAERRP TICLPPGAT GLHNLGNTC FMNAALQAV SNTRPLTLY FQRDGQLCE LNSVNPLGT KGMVARRYA ELCRELWAG STRSVAPLK LRYCVTKHA PHLGGGGQH DSQELLAWL LDALHEDLN RVARKQYSE LKDSGGRPD EVVAEEAWV QHLARDHSI ITDLFYGQL KSKVTCQTC GHESVRFDP FNLLSLPLP MESYTLCEV LVVRLNGET PIKYGLRLN SEAKYIELK QQLERLCNI KTERLLLAE LAYSQIRQV LNDDVRINP GTATELYAY ELPEDHQET EEISNDVDV EVAVSDSPL ARSPEVRGG SALCMPNIL CFKNRQKTD SHLMNGGVV LKKASIISH SFSDTSSTS SKFPSYLIA LHRKCVRQE TYFLSQQKS KPSLFGSPL LLGFRPNST CQSLYEAVW GQVTRFLSP LPQSDQTNH ATDCDDSLG YEFPFTLKA VLQGGQICA ICHWTQFCR GCTLPCSND LVVDHCRGN LNGMCIAID WDPTALHLR YQSNREKLW IEDESVSRC QKLHTEPID LDNCLKAFT SEERLETKY HCSNCQDKQ PATKKLQIW RLPPILIIH LKRFDCVNN KWVKTQKVV NFPFKDFDP TAYLASVPQ ETILRHLQL REKRQDLES SCDSQTSVK TIEESTETN CTTNEHSKL LTTDNKPKV QLNKVRARL ESTSLLKTP IIDEHLKDY HQHKLLPGQ DPFDLKYQL YAVVSHSGM LNGGHYISY ACNPNGNWY CYNDSSCRE VLTEIVEPV KPKKSTCTP LRRRKNTQS SEKEEETCN TDPKETTCD DTTVTKPEP NYKKCAYSD VKTPKIDTS SAYILFYER SGLDYKPYL PEVVSNGQI VPEVELDEN ESELRKQLC SIQ
- >TC033410 RA
atgggagca aaggactcg aaacggtcg tgcttgccg tacgaggat gccgtcaag cgaatgtct gaggaggaa tacaagcgg atatgcgag gcgttcaag cggctgagc ggcggcaac cagctcctg agcaagcag agcttcatc ctgcacgtg ctgggcgat ggggtgccc caggcgatc gccgagtgg ctgtacctc gcctgcggc ggcacgccg cgcggcatc gccctcaag gacctcgtc tgcggcctg gtgctgctc accaagggc aagcaggac gagaaaatt cgcttcctg ttcacgctg tattgcaac gattcggga acgcatatc accaggagc gagttctac agcgccttg caaaccgag ggcacgctg ccgcccgat aagcccaac aggcccaac atcctgtgg gagaggacg atgctctcg ctgttcccc gtgggacag gatcgagtg acctatgag catttcagt gcttggata cagtacaac aaggaggcg acgatcttg tccaagtgg ttactctca aacccttgc gtatcgcta agttcggaa gtggaagcg ccaactttt taccagaca ttggccggt gtgacacat ttggaagaa caagatatt tgcgaatta gagaaatgt ttttggaca cttcaaggc tcttcacct tcgtgccat ttagattta aattgtgtc cggtcgatg gtttgtccc ccgattccg ttgaccgca tgcgaggga ttattttta gcgctcgat gtgaacaga gacggccat atcgatttc aaagaattg tgctgtggg atttcggcg gcgtgtcgt ggaccaata gctgaacga atgaaattt tgtttcaaa atcttcgat atggataga gattgttat ctgaatccg gacgagctt caacatatg attcatact ttgatgctt gttgcgaat gaaaataac aagagattt aacggttac gatgggaat tttgatagt gataaggaa gaggatcat agtaaaatt ttgcaatct ttgactcaa agacttgac gagagaggg ggcttgagt caggaggag tttttggtt tggggtgtt gaagataat ggattggtt gagccgatg ttggagttg ttgtttcaa gtttgtcac gtttcgttt gttttgagg ccggcgtgt agacaccac gagcacgat attgtgacg ggatggctg gaacgcgaa gagcgtcgt ggctaccac gtgggccaa ttctggtac ctcatctcg tccgaatgg tggcaaaac tggctaagc tacacgacc gcccctcgc aacaacctc gaccactgc acgtgccgc accgccacc gacccgcgc ctcgtcgaa gagggcatc gtctgcgac gagagcctc atcagcaac ggcaccgat tcgaacgaa ttcaactcg aacagcatg gaatcgatg ggcgacctc ctcggcaaa ggcgacacc tgcagcatt gcctcgagt tcgggcgtt tcgagcagt tcgggcaca acgggctcg aaacgaagt caaggcatg ccgggaccc atcgacaat tcgaacctc gttgccgaa ccgttgcat aaagtgaga acgctgacg ggcgaaggc gggagatta aagcgggat acaccgttg gtgcaacat cgggatttt gaactggtt cccgattcg ctgtggaag gcgttggcg cattggtat gggggtccg ttgccgttg ccgcggcag gtgattttg ccgccgggg agttgcgat gttgagttg gagttgtat ccgttgaat ttgagtatt tggatgcat cagtcgcaa gcggtgggg tcggcggcg tcttggagc agtgtggtt ggaggctat ggagccgcc gccctcaac accgccggc ataaccacc tcccccatc tcctcccct cgccgttac ctcgcccac accgccgcc ttcagccgc ctcgccaac gtccgccaa gtgatcgaa ttcctctgt ctccgactc agcctccgt gtggaagac gtccgattg tggcacatc agggacacc accaccctc ctcgaagac gagaacgcc accctccaa gacctgagc gtcatcgac ggcgataaa atcctcctg gaggtgcgc aataaggac ctgacatgg cccgaagaa cttggtgct ttggtcgcc ggcggtcag acaacgctc agtgccgaa cgtcgaccg acaatttgt ctgccacct ggtgctaca ggcttgcat aatctcggc aatacgtgt tttatgaat gcggcactg caagcggtt tcaaatacg cgaccgtta acgttgtat tttcaaagg gatggtcag ttgtgcgaa ttgaatagt gtgaatccg ttgggtacg aagggaatg gtggcgagg cgatatgcc gagttgtgt agggagttg tgggccggg tcgacgagg agtgtggcg ccgttgaag ttgaggtat tgtgtcacc aaacatgcg ccgcatttg ggaggcgga ggacaacat gattcgcag gagcttctg gcatggcta ctggacgcc ctccacgag gacttaaac agggtagca cgtaaacaa tactcagaa ctgaaagat tccggagga cgtccggac gaagtggtc gctgaggag gcatgggtt caacatttg gcccgagat cattcgatt ataaccgat ctattttac ggccaatta aaaagcaaa gtcacttgt caaacgtgc ggccacgaa tcggtccga ttcgaccca ttcaacttg ttaagtttg ccattaccg atggaaagt tatacgtta tgcgaagtt ttagttgta agattaaac ggcgaaacg ccgataaaa tacggcctc cgcctcaac tccgaagcg aaatatatc gaactgaag caacagctc gaacgtctg tgcaatatc aaaaccgaa cgcctgctt ctggccgaa cttgcctat tcgcaaatc agacaagtg ttgaacgac gatgtccgg ataaatccg ggcacggcg accgaattg tacgcttat gagttacca gaggatcat caagaaacc gaagagata agtaacgat gtcgatgtg gaggttgcc gtttccgat agtccattg gcccgcagt cctgagaac aggcaaaaa accgatagt catctaatg aatggagga gttgtcctg aaaaaagcc tcaatcata tcacattcc ttttcggac acttcctca acatcgtca aaatttcca agttattta atagcctta catcggaaa tgtgtccga caagaaacc tactttctc tcacaacag aaaagcaaa ccgagttta tttggttca ccactttta cttggtttt cgtccaaat agtacgtgt cagagctta tacgaagcg gtttgggga caagtaacc aggtttctg agccctctg ccgcagagc gaccaaact aaccatgcc acagactgt gatgactcc ttgggatac gaattccct tttacgctg aaggccgtt cttcaaggc ggccaaatt tgtgcaata tgtcactgg acgcagttt tgtcgtggt tgtactcta ccttgtagc aatgatttg gttgtggac cattgtcga gggaatttg aatgggatg tgtattgcg atcgattgg gatccgacc gctttgcat ttgaggtat cagagcaat agggaaaag ttgtggata gaagatgag tctgttagt agatgtcag aagttgcat acggaaccc attgatttg gataattgt ttaaaagcg ttcacgtct gaagaaaga ctagaaaca aagtatcat tgttcaaat tgtcaggat aaacaaccg gcgacgaag aaattgcaa atttggcgg ttgccaccg atattgata atccatttg aagcgcttc gattgtgtg aacaacaaa tgggtaaaa acccaaaaa gtggtaaat ttcccattc aaagatttc gatccaacg gcatatctc gcatccgta ccgcaagaa acaatttta cgtcatcta caactacgc gaaaaacgt caagatctg gaaagttca tgtgatagt caaacatcg gttaaaaca atcgaagaa agcaccgaa acaaattgt acaacaaac gaacattcg aaactttta acaaccgac aacaaaccg aaagtgcaa ctgaacaaa gtcagagct cgcctagaa tcaaccagt ttgttgaaa acaccaatc atcgacgag catctcaag gactaccat cagcataaa ttattaccc ggacaggat cccttcgat ctgaaatac caactttat gctgttgtg agtcattca gggatgtta aatgggggt cattacatc tcctatgct tgcaatccg aacgggaac tggtactgt tacaatgac agttcgtgc cgggaggtt ttaaccgaa atagtagaa ccggttaaa ccgaaaaag tcgacttgt acgccactg cgtcgccgc aaaaacacg caatctagc gagaaggag gaagagacc tgtaatacc gacccgaag gaaaccact tgcgatgac accaccgtt actaaaccg gaaccgaat tacaaaaaa tgtgcttat agtgacgtg aaaaccccg aaaatcgac actagttcg gcgtatatt ttgttttac gaaaggtcg ggcctcgat tacaaaccg tatcttccc gaggtcgtt tcgaacggt caaatcgtg cctgaagtt gaactggac gagaatgaa tctgagtta cgtaagcaa ttgtgttcc attcagtaa
- >TC033410 RB
atgggagca aaggactcg aaacggtcg tgcttgccg tacgaggat gccgtcaag cgaatgtct gaggaggaa tacaagcgg atatgcgag gcgttcaag cggctgagc ggcggcaac cagctcctg agcaagcag agcttcatc ctgcacgtg ctgggcgat ggggtgccc caggcgatc gccgagtgg ctgtacctc gcctgcggc ggcacgccg cgcggcatc gccctcaag gacctcgtc tgcggcctg gtgctgctc accaagggc aagcaggac gagaaaatt cgcttcctg ttcacgctg tattgcaac gattcggga acgcatatc accaggagc gagttctac agcgccttg caaaccgag ggcacgctg ccgcccgat aagcccaac aggcccaac atcctgtgg gagaggacg atgctctcg ctgttcccc gtgggacag gatcgagtg acctatgag catttcagt gcttggata cagtacaac aaggaggcg acgatcttg tccaagtgg ttactctca aacccttgc gtatcgcta agttcggaa gtggaagcg ccaactttt taccagaca ttggccggt gtgacacat ttggaagaa caagatatt tgcgaatta gagaaatgt ttttggaca cttcaaggc tcttcacct tcgtgccat ttagattta aattgtgtc cggtcgatg gtttgtccc ccgattccg ttgaccgca tgcgaggga ttattttta gcgctcgat gtgaacaga gacggccat atcgatttc aaagaattg tgctgtggg atttcggcg gcgtgtcgt ggaccaata gctgaacga atgaaattt tgtttcaaa atcttcgat atggataga gattgttat ctgaatccg gacgagctt caacatatg attcatact ttgatgctt gttgcgaat gaaaataac aagagattt aacggttac gatgggaat tttgatagt gataaggaa gaggatcat agtaaaatt ttgcaatct ttgactcaa agacttgac gagagaggg ggcttgagt caggaggag tttttggtt tggggtgtt gaagataat ggattggtt gagccgatg ttggagttg ttgtttcaa gtttgtcac gtttcgttt gttttgagg ccggcgtgt agacaccac gagcacgat attgtgacg ggatggctg gaacgcgaa gagcgtcgt ggctaccac gtgggccaa ttctggtac ctcatctcg tccgaatgg tggcaaaac tggctaagc tacacgacc gcccctcgc aacaacctc gaccactgc acgtgccgc accgccacc gacccgcgc ctcgtcgaa gagggcatc gtctgcgac gagagcctc atcagcaac ggcaccgat tcgaacgaa ttcaactcg aacagcatg gaatcgatg ggcgacctc ctcggcaaa ggcgacacc tgcagcatt gcctcgagt tcgggcgtt tcgagcagt tcgggcaca acgggctcg aaacgaagt caaggcatg ccgggaccc atcgacaat tcgaacctc gttgccgaa ccgttgcat aaagtgaga acgctgacg ggcgaaggc gggagatta aagcgggat acaccgttg gtgcaacat cgggatttt gaactggtt cccgattcg ctgtggaag gcgttggcg cattggtat gggggtccg ttgccgttg ccgcggcag gtgattttg ccgccgggg agttgcgat gttgagttg gagttgtat ccgttgaat ttgagtatt tggatgcat cagtcgcaa gcggtgggg tcggcggcg tcttggagc agtgtggtt ggaggctat ggagccgcc gccctcaac accgccggc ataaccacc tcccccatc tcctcccct cgccgttac ctcgcccac accgccgcc ttcagccgc ctcgccaac gtccgccaa gtgatcgaa ttcctctgt ctccgactc agcctccgt gtggaagac gtccgattg tggcacatc agggacacc accaccctc ctcgaagac gagaacgcc accctccaa gacctgagc gtcatcgac ggcgataaa atcctcctg gaggtgcgc aataaggac ctgacatgg cccgaagaa cttggtgct ttggtcgcc ggcggtcag acaacgctc agtgccgaa cgtcgaccg acaatttgt ctgccacct ggtgctaca ggcttgcat aatctcggc aatacgtgt tttatgaat gcggcactg caagcggtt tcaaatacg cgaccgtta acgttgtat tttcaaagg gatggtcag ttgtgcgaa ttgaatagt gtgaatccg ttgggtacg aagggaatg gtggcgagg cgatatgcc gagttgtgt agggagttg tgggccggg tcgacgagg agtgtggcg ccgttgaag ttgaggtat tgtgtcacc aaacatgcg ccgcatttg ggaggcgga ggacaacat gattcgcag gagcttctg gcatggcta ctggacgcc ctccacgag gacttaaac agggtagca cgtaaacaa tactcagaa ctgaaagat tccggagga cgtccggac gaagtggtc gctgaggag gcatgggtt caacatttg gcccgagat cattcgatt ataaccgat ctattttac ggccaatta aaaagcaaa gtcacttgt caaacgtgc ggccacgaa tcggtccga ttcgaccca ttcaacttg ttaagtttg ccattaccg atggaaagt tatacgtta tgcgaagtt ttagttgta agattaaac ggcgaaacg ccgataaaa tacggcctc cgcctcaac tccgaagcg aaatatatc gaactgaag caacagctc gaacgtctg tgcaatatc aaaaccgaa cgcctgctt ctggccgaa cttgcctat tcgcaaatc agacaagtg ttgaacgac gatgtccgg ataaatccg ggcacggcg accgaattg tacgcttat gagttacca gaggatcat caagaaacc gaagagata agtaacgat gtcgatgtg gaggttgcc gtttccgat agtccattg gcccgcagt cctgaggta agaggtggt tcggccctt tgcatgccc aacattcta tgctttaag aacaggcaa aaaaccgat agtcatcta atgaatgga ggagttgtc ctgaaaaaa gcctcaatc atatcacat tccttttcg gacacttcc tcaacatcg tcaaaattt ccaagttat ttaatagcc ttacatcgg aaatgtgtc cgacaagaa acctacttt ctctcacaa cagaaaagc aaaccgagt ttatttggt tcaccactt ttacttggt tttcgtcca aatagtacg tgtcagagc ttatacgaa gcggtttgg ggacaagta accaggttt ctgagccct ctgccgcag agcgaccaa actaaccat gccacagac tgtgatgac tccttggga tacgaattc ccttttacg ctgaaggcc gttcttcaa ggcggccaa atttgtgca atatgtcac tggacgcag ttttgtcgt ggttgtact ctaccttgt agcaatgat ttggttgtg gaccattgt cgagggaat ttgaatggg atgtgtatt gcgatcgat tgggatccg accgctttg catttgagg tatcagagc aatagggaa aagttgtgg atagaagat gagtctgtt agtagatgt cagaagttg catacggaa cccattgat ttggataat tgtttaaaa gcgttcacg tctgaagaa agactagaa acaaagtat cattgttca aattgtcag gataaacaa ccggcgacg aagaaattg caaatttgg cggttgcca ccgatattg ataatccat ttgaagcgc ttcgattgt gtgaacaac aaatgggta aaaacccaa aaagtggta aatttccca ttcaaagat ttcgatcca acggcatat ctcgcatcc gtaccgcaa gaaacaatt ttacgtcat ctacaacta cgcgaaaaa cgtcaagat ctggaaagt tcatgtgat agtcaaaca tcggttaaa acaatcgaa gaaagcacc gaaacaaat tgtacaaca aacgaacat tcgaaactt ttaacaacc gacaacaaa ccgaaagtg caactgaac aaagtcaga gctcgccta gaatcaacc agtttgttg aaaacacca atcatcgac gagcatctc aaggactac catcagcat aaattatta cccggacag gatcccttc gatctgaaa taccaactt tatgctgtt gtgagtcat tcagggatg ttaaatggg ggtcattac atctcctat gcttgcaat ccgaacggg aactggtac tgttacaat gacagttcg tgccgggag gttttaacc gaaatagta gaaccggtt aaaccgaaa aagtcgact tgtacgcca ctgcgtcgc cgcaaaaac acgcaatct agcgagaag gaggaagag acctgtaat accgacccg aaggaaacc acttgcgat gacaccacc gttactaaa ccggaaccg aattacaaa aaatgtgct tatagtgac gtgaaaacc ccgaaaatc gacactagt tcggcgtat attttgttt tacgaaagg tcgggcctc gattacaaa ccgtatctt cccgaggtc gtttcgaac ggtcaaatc gtgcctgaa gttgaactg gacgagaat gaatctgag ttacgtaag caattgtgt tccattcag taa
- >TC033410 RA
tcagtcgtt gtgagtgtc gtgcgttga aaaccctcg taaaatgtt agaagactg cgtgaaatt gtgaaaatg ggagcaaag gactcgaaa cggtcgtgc ttgccgtac gaggatgcc gtcaagcga atgtctgag gaggaatac aagcggata tgcgaggcg ttcaagcgg ctgagcggc ggcaaccag ctcctgagc aagcagagc ttcatcctg cacgtgctg ggcgatggg gtgccccag gcgatcgcc gagtggctg tacctcgcc tgcggcggc acgccgcgc ggcatcgcc ctcaaggac ctcgtctgc ggcctggtg ctgctcacc aagggcaag caggacgag aaaattcgc ttcctgttc acgctgtat tgcaacgat tcgggaacg catatcacc aggagcgag ttctacagc gccttgcaa accgagggc acgctgccg cccgataag cccaacagg cccaacatc ctgtgggag aggacgatg ctctcgctg ttccccgtg ggacaggat cgagtgacc tatgagcat ttcagtgct tggatacag tacaacaag gaggcgacg atcttgtcc aagtggtta ctctcaaac ccttgcgta tcgctaagt tcggaagtg gaagcgcca actttttac cagacattg gccggtgtg acacatttg gaagaacaa gatatttgc gaattagag aaatgtttt tggacactt caaggctct tcaccttcg tgccattta gatttaaat tgtgtccgg tcgatggtt tgtcccccg attccgttg accgcatgc gagggatta tttttagcg ctcgatgtg aacagagac ggccatatc gatttcaaa gaattgtgc tgtgggatt tcggcggcg tgtcgtgga ccaatagct gaacgaatg aaattttgt ttcaaaatc ttcgatatg gatagagat tgttatctg aatccggac gagcttcaa catatgatt catactttg atgcttgtt gcgaatgaa aataacaag agatttaac ggttacgat gggaatttt gatagtgat aaggaagag gatcatagt aaaattttg caatctttg actcaaaga cttgacgag agagggggc ttgagtcag gaggagttt ttggtttgg ggtgttgaa gataatgga ttggttgag ccgatgttg gagttgttg tttcaagtt tgtcacgtt tcgtttgtt ttgaggccg gcgtgtaga caccacgag cacgatatt gtgacggga tggctggaa cgcgaagag cgtcgtggc taccacgtg ggccaattc tggtacctc atctcgtcc gaatggtgg caaaactgg ctaagctac acgaccgcc cctcgcaac aacctcgac cactgcacg tgccgcacc gccaccgac ccgcgcctc gtcgaagag ggcatcgtc tgcgacgag agcctcatc agcaacggc accgattcg aacgaattc aactcgaac agcatggaa tcgatgggc gacctcctc ggcaaaggc gacacctgc agcattgcc tcgagttcg ggcgtttcg agcagttcg ggcacaacg ggctcgaaa cgaagtcaa ggcatgccg ggacccatc gacaattcg aacctcgtt gccgaaccg ttgcataaa gtgagaacg ctgacgggc gaaggcggg agattaaag cgggataca ccgttggtg caacatcgg gattttgaa ctggttccc gattcgctg tggaaggcg ttggcgcat tggtatggg ggtccgttg ccgttgccg cggcaggtg attttgccg ccggggagt tgcgatgtt gagttggag ttgtatccg ttgaatttg agtatttgg atgcatcag tcgcaagcg gtggggtcg gcggcgtct tggagcagt gtggttgga ggctatgga gccgccgcc ctcaacacc gccggcata accacctcc cccatctcc tcccctcgc cgttacctc gcccacacc gccgccttc agccgcctc gccaacgtc cgccaagtg atcgaattc ctctgtctc cgactcagc ctccgtgtg gaagacgtc cgattgtgg cacatcagg gacaccacc accctcctc gaagacgag aacgccacc ctccaagac ctgagcgtc atcgacggc gataaaatc ctcctggag gtgcgcaat aaggacctg acatggccc gaagaactt ggtgctttg gtcgccggc ggtcagaca acgctcagt gccgaacgt cgaccgaca atttgtctg ccacctggt gctacaggc ttgcataat ctcggcaat acgtgtttt atgaatgcg gcactgcaa gcggtttca aatacgcga ccgttaacg ttgtatttt caaagggat ggtcagttg tgcgaattg aatagtgtg aatccgttg ggtacgaag ggaatggtg gcgaggcga tatgccgag ttgtgtagg gagttgtgg gccgggtcg acgaggagt gtggcgccg ttgaagttg aggtattgt gtcaccaaa catgcgccg catttggga ggcggagga caacatgat tcgcaggag cttctggca tggctactg gacgccctc cacgaggac ttaaacagg gtagcacgt aaacaatac tcagaactg aaagattcc ggaggacgt ccggacgaa gtggtcgct gaggaggca tgggttcaa catttggcc cgagatcat tcgattata accgatcta ttttacggc caattaaaa agcaaagtc acttgtcaa acgtgcggc cacgaatcg gtccgattc gacccattc aacttgtta agtttgcca ttaccgatg gaaagttat acgttatgc gaagtttta gttgtaaga ttaaacggc gaaacgccg ataaaatac ggcctccgc ctcaactcc gaagcgaaa tatatcgaa ctgaagcaa cagctcgaa cgtctgtgc aatatcaaa accgaacgc ctgcttctg gccgaactt gcctattcg caaatcaga caagtgttg aacgacgat gtccggata aatccgggc acggcgacc gaattgtac gcttatgag ttaccagag gatcatcaa gaaaccgaa gagataagt aacgatgtc gatgtggag gttgccgtt tccgatagt ccattggcc cgcagtcct gagaacagg caaaaaacc gatagtcat ctaatgaat ggaggagtt gtcctgaaa aaagcctca atcatatca cattccttt tcggacact tcctcaaca tcgtcaaaa tttccaagt tatttaata gccttacat cggaaatgt gtccgacaa gaaacctac tttctctca caacagaaa agcaaaccg agtttattt ggttcacca cttttactt ggttttcgt ccaaatagt acgtgtcag agcttatac gaagcggtt tggggacaa gtaaccagg tttctgagc cctctgccg cagagcgac caaactaac catgccaca gactgtgat gactccttg ggatacgaa ttccctttt acgctgaag gccgttctt caaggcggc caaatttgt gcaatatgt cactggacg cagttttgt cgtggttgt actctacct tgtagcaat gatttggtt gtggaccat tgtcgaggg aatttgaat gggatgtgt attgcgatc gattgggat ccgaccgct ttgcatttg aggtatcag agcaatagg gaaaagttg tggatagaa gatgagtct gttagtaga tgtcagaag ttgcatacg gaacccatt gatttggat aattgttta aaagcgttc acgtctgaa gaaagacta gaaacaaag tatcattgt tcaaattgt caggataaa caaccggcg acgaagaaa ttgcaaatt tggcggttg ccaccgata ttgataatc catttgaag cgcttcgat tgtgtgaac aacaaatgg gtaaaaacc caaaaagtg gtaaatttc ccattcaaa gatttcgat ccaacggca tatctcgca tccgtaccg caagaaaca attttacgt catctacaa ctacgcgaa aaacgtcaa gatctggaa agttcatgt gatagtcaa acatcggtt aaaacaatc gaagaaagc accgaaaca aattgtaca acaaacgaa cattcgaaa cttttaaca accgacaac aaaccgaaa gtgcaactg aacaaagtc agagctcgc ctagaatca accagtttg ttgaaaaca ccaatcatc gacgagcat ctcaaggac taccatcag cataaatta ttacccgga caggatccc ttcgatctg aaataccaa ctttatgct gttgtgagt cattcaggg atgttaaat gggggtcat tacatctcc tatgcttgc aatccgaac gggaactgg tactgttac aatgacagt tcgtgccgg gaggtttta accgaaata gtagaaccg gttaaaccg aaaaagtcg acttgtacg ccactgcgt cgccgcaaa aacacgcaa tctagcgag aaggaggaa gagacctgt aataccgac ccgaaggaa accacttgc gatgacacc accgttact aaaccggaa ccgaattac aaaaaatgt gcttatagt gacgtgaaa accccgaaa atcgacact agttcggcg tatattttg ttttacgaa aggtcgggc ctcgattac aaaccgtat cttcccgag gtcgtttcg aacggtcaa atcgtgcct gaagttgaa ctggacgag aatgaatct gagttacgt aagcaattg tgttccatt cagtaattt tctcttttt tttttttgt tgagttttg ttgaagttg cgagaaata tatttttgt aaatggtgt gccaccctt tgatttgtt ttcattttt tatatgatt atagtattg ttactccgt tttgcagat ctattattt atgaaagtg tatttatag atgttgata atattgtga tcttgaagc ctaaaattg taaaattag tctagagcg tgatgattt tttcgaaat gatgggatg gatggattt ttacgcgaa tcctggttt gattcaacc aaagatgta aacagattc gttcaaaag ttgactgtt ttggggtta tttatttta ctagtctga tattcttat cttattcca ctaagtttg taagatatt tataagtgt tgctttttt tgacacggt ttaaatata tttccatta ggtataata ttaatggtg ccattacac aataaaata aagcttgta ta
- >TC033410 RB
tcagtcgtt gtgagtgtc gtgcgttga aaaccctcg taaaatgtt agaagactg cgtgaaatt gtgaaaatg ggagcaaag gactcgaaa cggtcgtgc ttgccgtac gaggatgcc gtcaagcga atgtctgag gaggaatac aagcggata tgcgaggcg ttcaagcgg ctgagcggc ggcaaccag ctcctgagc aagcagagc ttcatcctg cacgtgctg ggcgatggg gtgccccag gcgatcgcc gagtggctg tacctcgcc tgcggcggc acgccgcgc ggcatcgcc ctcaaggac ctcgtctgc ggcctggtg ctgctcacc aagggcaag caggacgag aaaattcgc ttcctgttc acgctgtat tgcaacgat tcgggaacg catatcacc aggagcgag ttctacagc gccttgcaa accgagggc acgctgccg cccgataag cccaacagg cccaacatc ctgtgggag aggacgatg ctctcgctg ttccccgtg ggacaggat cgagtgacc tatgagcat ttcagtgct tggatacag tacaacaag gaggcgacg atcttgtcc aagtggtta ctctcaaac ccttgcgta tcgctaagt tcggaagtg gaagcgcca actttttac cagacattg gccggtgtg acacatttg gaagaacaa gatatttgc gaattagag aaatgtttt tggacactt caaggctct tcaccttcg tgccattta gatttaaat tgtgtccgg tcgatggtt tgtcccccg attccgttg accgcatgc gagggatta tttttagcg ctcgatgtg aacagagac ggccatatc gatttcaaa gaattgtgc tgtgggatt tcggcggcg tgtcgtgga ccaatagct gaacgaatg aaattttgt ttcaaaatc ttcgatatg gatagagat tgttatctg aatccggac gagcttcaa catatgatt catactttg atgcttgtt gcgaatgaa aataacaag agatttaac ggttacgat gggaatttt gatagtgat aaggaagag gatcatagt aaaattttg caatctttg actcaaaga cttgacgag agagggggc ttgagtcag gaggagttt ttggtttgg ggtgttgaa gataatgga ttggttgag ccgatgttg gagttgttg tttcaagtt tgtcacgtt tcgtttgtt ttgaggccg gcgtgtaga caccacgag cacgatatt gtgacggga tggctggaa cgcgaagag cgtcgtggc taccacgtg ggccaattc tggtacctc atctcgtcc gaatggtgg caaaactgg ctaagctac acgaccgcc cctcgcaac aacctcgac cactgcacg tgccgcacc gccaccgac ccgcgcctc gtcgaagag ggcatcgtc tgcgacgag agcctcatc agcaacggc accgattcg aacgaattc aactcgaac agcatggaa tcgatgggc gacctcctc ggcaaaggc gacacctgc agcattgcc tcgagttcg ggcgtttcg agcagttcg ggcacaacg ggctcgaaa cgaagtcaa ggcatgccg ggacccatc gacaattcg aacctcgtt gccgaaccg ttgcataaa gtgagaacg ctgacgggc gaaggcggg agattaaag cgggataca ccgttggtg caacatcgg gattttgaa ctggttccc gattcgctg tggaaggcg ttggcgcat tggtatggg ggtccgttg ccgttgccg cggcaggtg attttgccg ccggggagt tgcgatgtt gagttggag ttgtatccg ttgaatttg agtatttgg atgcatcag tcgcaagcg gtggggtcg gcggcgtct tggagcagt gtggttgga ggctatgga gccgccgcc ctcaacacc gccggcata accacctcc cccatctcc tcccctcgc cgttacctc gcccacacc gccgccttc agccgcctc gccaacgtc cgccaagtg atcgaattc ctctgtctc cgactcagc ctccgtgtg gaagacgtc cgattgtgg cacatcagg gacaccacc accctcctc gaagacgag aacgccacc ctccaagac ctgagcgtc atcgacggc gataaaatc ctcctggag gtgcgcaat aaggacctg acatggccc gaagaactt ggtgctttg gtcgccggc ggtcagaca acgctcagt gccgaacgt cgaccgaca atttgtctg ccacctggt gctacaggc ttgcataat ctcggcaat acgtgtttt atgaatgcg gcactgcaa gcggtttca aatacgcga ccgttaacg ttgtatttt caaagggat ggtcagttg tgcgaattg aatagtgtg aatccgttg ggtacgaag ggaatggtg gcgaggcga tatgccgag ttgtgtagg gagttgtgg gccgggtcg acgaggagt gtggcgccg ttgaagttg aggtattgt gtcaccaaa catgcgccg catttggga ggcggagga caacatgat tcgcaggag cttctggca tggctactg gacgccctc cacgaggac ttaaacagg gtagcacgt aaacaatac tcagaactg aaagattcc ggaggacgt ccggacgaa gtggtcgct gaggaggca tgggttcaa catttggcc cgagatcat tcgattata accgatcta ttttacggc caattaaaa agcaaagtc acttgtcaa acgtgcggc cacgaatcg gtccgattc gacccattc aacttgtta agtttgcca ttaccgatg gaaagttat acgttatgc gaagtttta gttgtaaga ttaaacggc gaaacgccg ataaaatac ggcctccgc ctcaactcc gaagcgaaa tatatcgaa ctgaagcaa cagctcgaa cgtctgtgc aatatcaaa accgaacgc ctgcttctg gccgaactt gcctattcg caaatcaga caagtgttg aacgacgat gtccggata aatccgggc acggcgacc gaattgtac gcttatgag ttaccagag gatcatcaa gaaaccgaa gagataagt aacgatgtc gatgtggag gttgccgtt tccgatagt ccattggcc cgcagtcct gaggtaaga ggtggttcg gccctttgc atgcccaac attctatgc tttaagaac aggcaaaaa accgatagt catctaatg aatggagga gttgtcctg aaaaaagcc tcaatcata tcacattcc ttttcggac acttcctca acatcgtca aaatttcca agttattta atagcctta catcggaaa tgtgtccga caagaaacc tactttctc tcacaacag aaaagcaaa ccgagttta tttggttca ccactttta cttggtttt cgtccaaat agtacgtgt cagagctta tacgaagcg gtttgggga caagtaacc aggtttctg agccctctg ccgcagagc gaccaaact aaccatgcc acagactgt gatgactcc ttgggatac gaattccct tttacgctg aaggccgtt cttcaaggc ggccaaatt tgtgcaata tgtcactgg acgcagttt tgtcgtggt tgtactcta ccttgtagc aatgatttg gttgtggac cattgtcga gggaatttg aatgggatg tgtattgcg atcgattgg gatccgacc gctttgcat ttgaggtat cagagcaat agggaaaag ttgtggata gaagatgag tctgttagt agatgtcag aagttgcat acggaaccc attgatttg gataattgt ttaaaagcg ttcacgtct gaagaaaga ctagaaaca aagtatcat tgttcaaat tgtcaggat aaacaaccg gcgacgaag aaattgcaa atttggcgg ttgccaccg atattgata atccatttg aagcgcttc gattgtgtg aacaacaaa tgggtaaaa acccaaaaa gtggtaaat ttcccattc aaagatttc gatccaacg gcatatctc gcatccgta ccgcaagaa acaatttta cgtcatcta caactacgc gaaaaacgt caagatctg gaaagttca tgtgatagt caaacatcg gttaaaaca atcgaagaa agcaccgaa acaaattgt acaacaaac gaacattcg aaactttta acaaccgac aacaaaccg aaagtgcaa ctgaacaaa gtcagagct cgcctagaa tcaaccagt ttgttgaaa acaccaatc atcgacgag catctcaag gactaccat cagcataaa ttattaccc ggacaggat cccttcgat ctgaaatac caactttat gctgttgtg agtcattca gggatgtta aatgggggt cattacatc tcctatgct tgcaatccg aacgggaac tggtactgt tacaatgac agttcgtgc cgggaggtt ttaaccgaa atagtagaa ccggttaaa ccgaaaaag tcgacttgt acgccactg cgtcgccgc aaaaacacg caatctagc gagaaggag gaagagacc tgtaatacc gacccgaag gaaaccact tgcgatgac accaccgtt actaaaccg gaaccgaat tacaaaaaa tgtgcttat agtgacgtg aaaaccccg aaaatcgac actagttcg gcgtatatt ttgttttac gaaaggtcg ggcctcgat tacaaaccg tatcttccc gaggtcgtt tcgaacggt caaatcgtg cctgaagtt gaactggac gagaatgaa tctgagtta cgtaagcaa ttgtgttcc attcagtaa ttttctctt ttttttttt tgttgagtt ttgttgaag ttgcgagaa atatatttt tgtaaatgg tgtgccacc ctttgattt gttttcatt ttttatatg attatagta ttgttactc cgttttgca gatctatta tttatgaaa gtgtattta tagatgttg ataatattg tgatcttga agcctaaaa ttgtaaaat tagtctaga gcgtgatga ttttttcga aatgatggg atggatgga tttttacgc gaatcctgg tttgattca accaaagat gtaaacaga ttcgttcaa aagttgact gttttgggg ttatttatt ttactagtc tgatattct tatcttatt ccactaagt ttgtaagat atttataag tgttgcttt ttttgacac ggtttaaat atatttcca ttaggtata atattaatg gtgccatta cacaataaa ataaagctt gtata
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