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Gene information
General information
TC015071 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3761067..3762654
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015071
    iBeetle screen iB_02393

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late number decreased - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015071 RA
      MSLGFESLV SLVSASWKN LTVRVQEYT KETVEHLIV 
      AVSVYIIVC VFLYLSISR WKIIHFKKD VKNPHRVLL 
      VIAHPDDEC MFFGPTVLN FTKQNQCKV FLMCLSTGQ 
      NYGLGKTRK QELYKSCRM LGIDDSCIT VCNHTNLPD 
      QMGAKWPIE LVAKLILNH VETYSIDTL VTFDKHGVS 
      YHLNHCSIY YAIAHLSIE KKLPKECSV YVLETVNLL 
      RKYWLLLDI PVSFLLSRI RYLVTSADR AIIHKAMKQ 
      HQSQLVWFR RLYMLFSRY MLINSLQLM ELSDIELDL 
      EIDD
    CDS sequence
    • >TC015071 RA
      atgagtcta ggtttcgaa tcgttggtg tccctcgtc 
      tcagcctct tggaagaat ctaaccgtt cgagtccag 
      gaatacacg aaagaaacc gtggagcat ttaatcgta 
      gccgtgtcc gtgtacatc atagtatgc gtgtttttg 
      tacctttcc atctctcgc tggaagata atccacttc 
      aaaaaagac gtaaaaaac ccccatcgt gttttacta 
      gtaatcgca catcctgac gacgaatgc atgtttttt 
      ggccccact gttttgaac ttcaccaaa cagaaccaa 
      tgtaaagtg ttcctgatg tgtctttcg acagggcaa 
      aattacgga ctggggaaa acccgcaaa caggaattg 
      tacaaatcg tgtcgcatg ctgggcatt gacgatagc 
      tgtatcacg gtgtgcaac cacacgaac ttgcccgat 
      caaatgggg gccaaatgg ccgattgaa ttggtcgct 
      aaattgatt ttaaaccac gtggagacc tacagcatt 
      gacacgctt gtcaccttt gataaacac ggagttagc 
      tatcatctc aatcactgc agtatttac tacgcgatc 
      gctcatctc agcattgag aaaaaattg cccaaggaa 
      tgttctgtt tatgttcta gagactgtt aatttgttg 
      aggaagtat tggctcctg ttggatatt cccgtctcg 
      tttcttttg tccaggata aggtattta gtgacttct 
      gcagaccgc gctataatc cacaaggcc atgaaacag 
      catcagtcc caattagtt tggtttcga cgtctctat 
      atgcttttc tcaaggtat atgttaatt aattcgcta 
      cagttaatg gaattgtcg gatatagaa ttagattta 
      gaaatagac gactaa
    mRNA sequence
    • >TC015071 RA
      aatccctag aaatgggga cgtcaacct aacctcaaa 
      attatgccc tgtgccaat ttctccgcc agaaaatct 
      aaaaatacc gcaaatgct cggtaaatc tatttcgag 
      cgtgacttc atgactgag atgagccaa gcagcgcct 
      tggcatgag tctaggttt cgaatcgtt ggtgtccct 
      cgtctcagc ctcttggaa gaatctaac cgttcgagt 
      ccaggaata cacgaaaga aaccgtgga gcatttaat 
      cgtagccgt gtccgtgta catcatagt atgcgtgtt 
      tttgtacct ttccatctc tcgctggaa gataatcca 
      cttcaaaaa agacgtaaa aaaccccca tcgtgtttt 
      actagtaat cgcacatcc tgacgacga atgcatgtt 
      ttttggccc cactgtttt gaacttcac caaacagaa 
      ccaatgtaa agtgttcct gatgtgtct ttcgacagg 
      gcaaaatta cggactggg gaaaacccg caaacagga 
      attgtacaa atcgtgtcg catgctggg cattgacga 
      tagctgtat cacggtgtg caaccacac gaacttgcc 
      cgatcaaat gggggccaa atggccgat tgaattggt 
      cgctaaatt gattttaaa ccacgtgga gacctacag 
      cattgacac gcttgtcac ctttgataa acacggagt 
      tagctatca tctcaatca ctgcagtat ttactacgc 
      gatcgctca tctcagcat tgagaaaaa attgcccaa 
      ggaatgttc tgtttatgt tctagagac tgttaattt 
      gttgaggaa gtattggct cctgttgga tattcccgt 
      ctcgtttct tttgtccag gataaggta tttagtgac 
      ttctgcaga ccgcgctat aatccacaa ggccatgaa 
      acagcatca gtcccaatt agtttggtt tcgacgtct 
      ctatatgct tttctcaag gtatatgtt aattaattc 
      gctacagtt aatggaatt gtcggatat agaattaga 
      tttagaaat agacgacta aatttatat cactctaga 
      cgaaacaaa gtttcaact tttatatat caacttgta 
      aatacaaac ctacctctt gatttttgt tgtttttgt 
      gccaaagtc tatgcttct ttgtaagtt tttatgaat 
      tttaataaa attatgttt ctatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY