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Gene information
General information
TC015063 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3894855..3902092
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015063
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02389

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature dorsal randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015063 RA
      MSATAQTNG KKSSNSSIV NTPASTPVT NGNSQTISQ 
      STPPPPNNN APKYGTLVP NRIFVGGIS ANTTEGELL 
      QLFSSYGTV KAAKIIQDR AGVSKGYGF ITFESEDDA 
      KRPLKDADN IVLKERKLN IAPAIKKQP FNRAFDAAS 
      PSTVAPGNT AQYFFPPSP GVPYFQGGV TYYQQPPAA 
      PGDPNSQTT VYQAPPVYP AQTGQPQAP TYPPMMFPH 
      HIYMPQQYA MPPMPYDYN YYPNNGGGA QYMVGGQNG 
      GPGGGGSGG NSQGGPPMP PPNSPPRPP CYNAQLPPY 
      TPDPVYYNM PVYGGPVEG APLYPEGLD MSNGVGYPE 
      VSQLLGEFL HGFDGCTDI HQGPYPMAD PTQDINDSA 
      PPPLPPPIA TPLTADHAP LYEHETNDA DNSRQSKTP 
      VVSLLSMEQ HQEKDFVSM QGGRRVKPQ PPPPPPPQQ 
      QPMIIPHFA QNQAPPPIY YNFNGPPVR SHQIPPYNN 
      NNIKTTNGY RKPRTFNSA SANRANDNS SRSSLRTDS 
      NSSANAAVD ENKNEDKQS PNSFHLYNG SRTYQNSLG 
      RRINNNKKD TALDNNNKT KSNFVVQSQ QQLRNNGQQ 
      SYVLTSASA ASVVRRNKR STRRNVGQS GGSLSEIGA 
      GDAPLANND VTDTCKKME TLKM
    CDS sequence
    • >TC015063 RA
      atgtcggcc accgctcaa accaacggc aagaagagc 
      tcgaacagt tccattgtg aacacccca gccagcact 
      ccggtgact aacggcaat tcgcaaacg atctcccag 
      agcacccct ccgcccccg aacaacaac gcccccaag 
      tacggcacc ctggtcccc aaccgaatc ttcgtcggc 
      ggcatctcc gccaacacc accgaaggc gagctcttg 
      cagctcttc agcagttac gggaccgtc aaagcggcc 
      aaaatcatc caagacagg gcaggcgtc tccaaaggc 
      tacgggttc ataacgttc gaaagcgaa gacgatgcc 
      aaacgcccc ctcaaagac gccgacaac atcgtcctc 
      aaggaaaga aaactgaac attgcgcca gcaatcaag 
      aaacaaccg tttaatcgg gcctttgac gccgccagt 
      ccctcgact gtcgcgcca gggaacacg gcacagtac 
      tttttcccg ccgagcccc ggagtgccc tactttcaa 
      gggggcgtc acttactac caacagcca ccggcagcc 
      cccggcgat cccaactca caaacgacc gtctatcag 
      gctcctcct gtatatccg gcccaaacc ggacaacca 
      caagcccca acttaccca ccgatgatg ttccctcat 
      cacatctat atgccacaa cagtacgcc atgccaccg 
      atgccgtac gactacaac tactaccct aacaacgga 
      ggaggagcc cagtacatg gtcggcggt cagaatggg 
      ggcccagga ggcggcgga tccggcgga aattctcag 
      ggcggcccc ccaatgccc cccccgaac agcccccct 
      cgaccgcct tgctacaac gcccaattg cccccgtac 
      acaccggac ccagtatac tacaacatg ccagtatac 
      ggcggtccg gtcgagggg gcccccctc tatccagaa 
      ggtctggac atgtccaac ggcgtcggt tatcctgag 
      gttagtcag ttattaggc gagtttttg cacggcttt 
      gacggatgc acggatatt catcagggc ccctatcct 
      atggccgac cccacccaa gacataaac gactccgcc 
      ccaccccca ctaccacca ccaatcgcc acacctctc 
      accgccgac cacgcccca ctatacgaa cacgaaacg 
      aacgacgcc gacaacagc cgacaaagc aaaacgccg 
      gtcgtgtcg ctgctgtcg atggaacaa caccaagag 
      aaagacttc gtttcgatg caaggcgga cgccgcgtc 
      aaaccccaa ccaccccca ccaccaccg ccacaacaa 
      caaccaatg attataccg catttcgcc caaaatcaa 
      gccccgccc cccatctac tacaacttc aacggaccg 
      cccgtacga tctcatcaa atcccgcct tacaacaac 
      aacaacatt aaaacgacg aacggttat cgcaaaccg 
      cgcactttt aactccgcc tccgcgaat cgagccaat 
      gacaattcg tcgagatca agtctccgg actgattcg 
      aattcgtcg gcgaacgcc gccgtcgac gaaaacaaa 
      aacgaagat aaacaatcg ccaaacagt tttcatttg 
      tataacggt tcgagaacg tatcagaat tcgctagga 
      cgacgcatc aacaacaac aagaaggac accgccttg 
      gacaacaac aacaaaact aaaagtaat ttcgttgtt 
      caatcgcag caacagctg aggaacaac ggccagcag 
      agctacgtg ctgacgtcg gcgtcggca gcgtcggtc 
      gtcaggcgg aacaaaaga tcgacgcgt cgcaacgtc 
      ggccaatcg ggaggttcg ctgagcgaa atcggcgcc 
      ggtgacgcc cccctagcc aacaacgat gttacagat 
      acctgtaaa aaaatggaa acactgaaa atgtga
    mRNA sequence
    • >TC015063 RA
      tcgacgcca ttttatcga tggctacgt tttgtagtg 
      aaaacgact ttagttttt aagaatttc tgtgttcat 
      ttctctaaa ttcactcat gtttcttca taaatttac 
      ggtgttgtt gccacaagt agttttcct cagtgagtt 
      tgcttggta atcggtaaa atgtcggcc accgctcaa 
      accaacggc aagaagagc tcgaacagt tccattgtg 
      aacacccca gccagcact ccggtgact aacggcaat 
      tcgcaaacg atctcccag agcacccct ccgcccccg 
      aacaacaac gcccccaag tacggcacc ctggtcccc 
      aaccgaatc ttcgtcggc ggcatctcc gccaacacc 
      accgaaggc gagctcttg cagctcttc agcagttac 
      gggaccgtc aaagcggcc aaaatcatc caagacagg 
      gcaggcgtc tccaaaggc tacgggttc ataacgttc 
      gaaagcgaa gacgatgcc aaacgcccc ctcaaagac 
      gccgacaac atcgtcctc aaggaaaga aaactgaac 
      attgcgcca gcaatcaag aaacaaccg tttaatcgg 
      gcctttgac gccgccagt ccctcgact gtcgcgcca 
      gggaacacg gcacagtac tttttcccg ccgagcccc 
      ggagtgccc tactttcaa gggggcgtc acttactac 
      caacagcca ccggcagcc cccggcgat cccaactca 
      caaacgacc gtctatcag gctcctcct gtatatccg 
      gcccaaacc ggacaacca caagcccca acttaccca 
      ccgatgatg ttccctcat cacatctat atgccacaa 
      cagtacgcc atgccaccg atgccgtac gactacaac 
      tactaccct aacaacgga ggaggagcc cagtacatg 
      gtcggcggt cagaatggg ggcccagga ggcggcgga 
      tccggcgga aattctcag ggcggcccc ccaatgccc 
      cccccgaac agcccccct cgaccgcct tgctacaac 
      gcccaattg cccccgtac acaccggac ccagtatac 
      tacaacatg ccagtatac ggcggtccg gtcgagggg 
      gcccccctc tatccagaa ggtctggac atgtccaac 
      ggcgtcggt tatcctgag gttagtcag ttattaggc 
      gagtttttg cacggcttt gacggatgc acggatatt 
      catcagggc ccctatcct atggccgac cccacccaa 
      gacataaac gactccgcc ccaccccca ctaccacca 
      ccaatcgcc acacctctc accgccgac cacgcccca 
      ctatacgaa cacgaaacg aacgacgcc gacaacagc 
      cgacaaagc aaaacgccg gtcgtgtcg ctgctgtcg 
      atggaacaa caccaagag aaagacttc gtttcgatg 
      caaggcgga cgccgcgtc aaaccccaa ccaccccca 
      ccaccaccg ccacaacaa caaccaatg attataccg 
      catttcgcc caaaatcaa gccccgccc cccatctac 
      tacaacttc aacggaccg cccgtacga tctcatcaa 
      atcccgcct tacaacaac aacaacatt aaaacgacg 
      aacggttat cgcaaaccg cgcactttt aactccgcc 
      tccgcgaat cgagccaat gacaattcg tcgagatca 
      agtctccgg actgattcg aattcgtcg gcgaacgcc 
      gccgtcgac gaaaacaaa aacgaagat aaacaatcg 
      ccaaacagt tttcatttg tataacggt tcgagaacg 
      tatcagaat tcgctagga cgacgcatc aacaacaac 
      aagaaggac accgccttg gacaacaac aacaaaact 
      aaaagtaat ttcgttgtt caatcgcag caacagctg 
      aggaacaac ggccagcag agctacgtg ctgacgtcg 
      gcgtcggca gcgtcggtc gtcaggcgg aacaaaaga 
      tcgacgcgt cgcaacgtc ggccaatcg ggaggttcg 
      ctgagcgaa atcggcgcc ggtgacgcc cccctagcc 
      aacaacgat gttacagat acctgtaaa aaaatggaa 
      acactgaaa atgtgatta aaacgaggt ttgttgttg 
      acacttaag aaagggata atttattta ttaaaaaag 
      aaatgtttg cttgtatat aaaaaacac aaaaaaata 
      ataataaaa atgcaatat tatttaaat aatcgacaa 
      tattattgt tattaatac aaaagtgag atgaagccc 
      gtttagcat tggacttta ctattatga ttcctagct 
      taggttctt ttgtagata aagaagaga gaaaaaact 
      aagtcctac acatatttt taacaagta attgttatt 
      taatgtcta aggatgtgt ataaattag tttagtttc 
      gattatttt tttctaaac tcgatgact cttattcgc 
      aacaatcgt attttagtc gaggggaac tgtgtccta 
      tcttgaatt tgaggtgtt gtttgctcg atgccttat 
      ttcctctca acttgtacg ttctatagg ttagttttt 
      gatattgtt tttcccctt tgaaaacag tctttacaa 
      ttgacttta aaatcaaag tgagatttt tgctcaaaa 
      aggctgcca tcggttttt agtccaatt cgagtgaat 
      ctatcggct ctctaaatc acacaacac gcacttttg 
      ttgtaattc cctcccatt ttgttttgt tgtttgtcg 
      gaatatttc aattttagg ctgtgacgt taaattgtt 
      ataaatttg tagtgattt catcgaaat tcaaaattt 
      tttacggga cagattgtt acaaatcaa actattatt 
      attacacaa aatgtttca aaagagcct aaaatttat 
      tatgaatga tttttttcc aaagaagta ttttaggat 
      tagtcgtaa ggattgtag caatgtaag atagtaatt 
      tattaagta gtgtagata tttaggtgt catagttca 
      taaatcgag aattttttg gccaaaact aattacact 
      atttagtta attgtgctt accgttgcg ggtaagcaa 
      ccgagtctt gttgaaagt atattaaca aagtacctg 
      tattattaa atctacgct aaaatgaaa caaacaaaa 
      catttgtaa atacctatt tagttgata tacttgttg 
      atgtttgga aattttatc gaagaaagc gttttcgtt 
      gcgttattg gccaacgta gcttttaat ttataagta 
      ggtttaggg aagatcacc catgccccg cccctcaca 
      aaaacaaga gcaatatgt aaactctgt caattttag 
      tttgtatat ataataatc aaaaaactt gtcaaaaaa 
      aagaaatgc tctaaaaac ggttgtgca taaaattcg 
      tagtcgtaa agtatttag ctgtagttt aaatacacg 
      attgatcgg ctcccaaaa cgccgaata caaaaatac 
      aaactgaaa tgtgagcta tgataagcc aaatttttg 
      tgatagcgg cgaaagatg tgtttttaa aattcaaat 
      tttcccgcc tggggaaaa ttcgaattt taaaacaca 
      aatttccga cttaaaatg tgtgttcaa caacttgaa 
      atcttgtag gcatattat tagacttgc ttcgggagg 
      ctctgcggc ctcgtctca atagctgta acaaacaac 
      aaaaacgga aaaatagcg ttatggaac tatgaatgt 
      aaaataaaa cgcaatatt ttagaaatt atataactt 
      aagaaaaca gtgagaaat atttattaa ttacaataa 
      caattataa ttaattaac tattaaata agcatgtac 
      aaagtatct cagataata aatttaaat ttaagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY