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Gene information
General information
TC015052 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4209413..4219872
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015052
    iBeetle screen iB_02385

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015052 RA
      MAAIQNGVC SSHTGVAEF YNGKTVFIT GGTGFMGKV 
      LLEKLLRAC PGVAKIYLL IRPKKGQDA HERLKLLLC 
      SPLFDPIRK SRPSDLHKV LPIEGDITQ PELAISSND 
      RLTLARTVN IVFHSAATI KFDEKLKLS VTINMLGTQ 
      RLVELCKRM TNLEALVHV STAYCNCDR SEVKETIYP 
      PPMGPDQVT SLVDCLDES LLDSLTSRL VGNRPNTYT 
      FTKALAECW LKENKGDLP LVIVRPSIV LCSFGGPLK 
      GWVDNWNGP TGIIAAAGK GLFRTMLCD PEKIADLVP 
      VDMVINLML VAAWRIGTT KTKDMPIYN CSTGQRRPI 
      TWKHFVGLC FKYMRKHPF SDVTWYPDG TVTASRTLN 
      IINKYLLHW LPAYIMDGL VWMAGGKPI MVRIQDKLC 
      KAATCLEYF TMNEWHFDD ENVRILSQQ LNEKDREEF 
      CFDVAKIDW EQYVEDYVL GIRRFIFKE ESSSIPHAR 
      RQVSRLYWL YRCLQVASV MCMWHFLTL RYAPLRQLW 
      GNALRLLIH LARMLPFV
    CDS sequence
    • >TC015052 RA
      atggccgcc attcaaaac ggagtttgt tcgtcgcac 
      acaggcgtt gccgagttc tacaacggc aaaactgtt 
      ttcatcaca ggcggcacc ggcttcatg ggtaaagta 
      ctgctcgaa aagttgctc agggcgtgt ccaggagtg 
      gccaaaatc taccttctc atcaggccc aagaagggc 
      caagacgcg cacgaacgc ctaaaactg ctactgtgt 
      tcaccgctg tttgatccg attcggaag agtcgtccg 
      tcggatctg cacaaggtg ttacccatc gaaggcgat 
      ataacacag cctgaattg gcgataagt tcaaatgat 
      agattaaca ttggctaga accgtcaac attgttttt 
      catagcgca gcaacgata aaattcgac gaaaaattg 
      aaattatcg gtgactatt aatatgcta gggacgcaa 
      agattggtc gaattgtgc aaaagaatg acgaatttg 
      gaggctttg gtgcacgtg tccaccgcc tactgcaat 
      tgcgaccgt agcgaagta aaagagacc atttacccg 
      ccacctatg ggtcccgat caagtcacc tcattggtg 
      gattgttta gatgaaagc cttctcgat tcgttaacg 
      tcacgtctt gtgggaaat cggcccaat acttatacg 
      tttacgaaa gcattggcc gaatgctgg ttaaaagaa 
      aacaaaggc gatttacca ttggttatc gtacgacca 
      agtatagtc ttgtgcagt tttggtgga cctttaaaa 
      ggctgggtt gataattgg aacggtcca acgggtatt 
      atcgcggcg gctgggaaa ggactcttc cggacgatg 
      ttatgcgat ccggaaaaa atcgccgat ctagtgccc 
      gttgacatg gtcattaat ttaatgctg gtggcggcg 
      tggcgaatc ggaacgacc aaaaccaaa gacatgccc 
      atttataat tgtagcaca ggtcaaagg aggccaata 
      acgtggaaa cattttgtc ggtttgtgc ttcaaatac 
      atgaggaaa cacccattt tcggatgtc acgtggtat 
      ccggatggg acggtgacg gcgtcccga actttgaat 
      ataattaat aagtatttg ttgcattgg ttaccggcg 
      tatattatg gatgggttg gtttggatg gctgggggg 
      aagcccata atggttcgt atccaggat aagctgtgc 
      aaggcggcc acgtgcctg gagtacttc acgatgaac 
      gagtggcat ttcgacgac gagaacgtg cgcattttg 
      agccaacag ttgaacgag aaagatcgc gaagagttt 
      tgttttgac gtggcaaag atcgattgg gaacagtac 
      gtggaggac tatgttctc ggcatccga cgtttcatt 
      ttcaaggag gagtcatca tcaatacca cacgcgcgc 
      cgccaagtg tcgcgtctc tattggttg tacagatgt 
      ttgcaggtg gcgtcggtg atgtgcatg tggcatttt 
      ctaacgtta cgttacgcc cccttgcgg cagttgtgg 
      ggcaacgcc ctcagactg ctcattcac ttagcccga 
      atgctgcca tttgtatag
    mRNA sequence
    • >TC015052 RA
      aatcgacgt gttaatgaa tattcgtgt tggcttttt 
      ggaaaaatc gcgtcgccc gtgccggcc ttccatcgt 
      tagaatcac agcgaagaa gaaagaaat gaactgaag 
      acgacagaa accgctact ctcttccaa aaaaagcac 
      aaatttttc acccgactt cctgttaga tgttggacg 
      ttgtttttt cgaattttt attaaatgg tgtccgtaa 
      gaaacgaaa tggccgcca ttcaaaacg gagtttgtt 
      cgtcgcaca caggcgttg ccgagttct acaacggca 
      aaactgttt tcatcacag gcggcaccg gcttcatgg 
      gtaaagtac tgctcgaaa agttgctca gggcgtgtc 
      caggagtgg ccaaaatct accttctca tcaggccca 
      agaagggcc aagacgcgc acgaacgcc taaaactgc 
      tactgtgtt caccgctgt ttgatccga ttcggaaga 
      gtcgtccgt cggatctgc acaaggtgt tacccatcg 
      aaggcgata taacacagc ctgaattgg cgataagtt 
      caaatgata gattaacat tggctagaa ccgtcaaca 
      ttgtttttc atagcgcag caacgataa aattcgacg 
      aaaaattga aattatcgg tgactatta atatgctag 
      ggacgcaaa gattggtcg aattgtgca aaagaatga 
      cgaatttgg aggctttgg tgcacgtgt ccaccgcct 
      actgcaatt gcgaccgta gcgaagtaa aagagacca 
      tttacccgc cacctatgg gtcccgatc aagtcacct 
      cattggtgg attgtttag atgaaagcc ttctcgatt 
      cgttaacgt cacgtcttg tgggaaatc ggcccaata 
      cttatacgt ttacgaaag cattggccg aatgctggt 
      taaaagaaa acaaaggcg atttaccat tggttatcg 
      tacgaccaa gtatagtct tgtgcagtt ttggtggac 
      ctttaaaag gctgggttg ataattgga acggtccaa 
      cgggtatta tcgcggcgg ctgggaaag gactcttcc 
      ggacgatgt tatgcgatc cggaaaaaa tcgccgatc 
      tagtgcccg ttgacatgg tcattaatt taatgctgg 
      tggcggcgt ggcgaatcg gaacgacca aaaccaaag 
      acatgccca tttataatt gtagcacag gtcaaagga 
      ggccaataa cgtggaaac attttgtcg gtttgtgct 
      tcaaataca tgaggaaac acccatttt cggatgtca 
      cgtggtatc cggatggga cggtgacgg cgtcccgaa 
      ctttgaata taattaata agtatttgt tgcattggt 
      taccggcgt atattatgg atgggttgg tttggatgg 
      ctgggggga agcccataa tggttcgta tccaggata 
      agctgtgca aggcggcca cgtgcctgg agtacttca 
      cgatgaacg agtggcatt tcgacgacg agaacgtgc 
      gcattttga gccaacagt tgaacgaga aagatcgcg 
      aagagtttt gttttgacg tggcaaaga tcgattggg 
      aacagtacg tggaggact atgttctcg gcatccgac 
      gtttcattt tcaaggagg agtcatcat caataccac 
      acgcgcgcc gccaagtgt cgcgtctct attggttgt 
      acagatgtt tgcaggtgg cgtcggtga tgtgcatgt 
      ggcattttc taacgttac gttacgccc ccttgcggc 
      agttgtggg gcaacgccc tcagactgc tcattcact 
      tagcccgaa tgctgccat ttgtataga taataatta 
      ggtagggcg tcgcgacgc ttttcctct tcgccaatt 
      ggtcccact ttcccctat tttatcgtg tgtcgtgag 
      tgtattgta gataattgt atgttacgt cataagtgg 
      gtgaagggg aaaagcatc gattgattg attgattga 
      ttcgtttgt ggttttttg cttttgaag ctcattcca 
      gctgtgtaa gtgaaaccg ttcttaggg aattttcat 
      ttttcacca ggggaatgt tactattcc tggttttaa 
      gcgaaaatg ttgcgtcgt ttatttatt tgttatttt 
      taccattgt actagtctt atagcaaac taaagggat 
      cgcttagtt gataaataa aattattta ttattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY