Status
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Gene information
General information
TC015051 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4220848..4225092
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015051
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02384

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015051 RA
      MSSSEIVSF YTGKNFFIT GASGFVGIC LLEKILRVI 
      PNHGQIYLL LRPKKGKAI NERLEEIKK NQIFEKLLA 
      NRSVEDVFR GVQAIAGDV GQDNLGISP EDRRLLVEN 
      VNVIIHSAA TLDFGDTLK TTVNINLLG TRRITQLAK 
      ECRQLKVLT HVSSAYVNS YRLECEEII YDKPCDSEE 
      LISLVEKLS EAELEKQTP EILKDHPNT YTITKHLAE 
      HEIKNCETI FPCTIVRPS MIIGAWKEP IPGWTISKN 
      GPQGFLMGA AKGVIRRLP VGKSLIYDY IPVDVVVNN 
      LLAAGYSAA THNAKQVEV YHCTSSTRN PFSWILIED 
      RVNSFLHEY PLKSAVWYP YLKFLPSVT WYKISAFFV 
      HILPAILLD FVTRVAGGR PILMKLHRN VNTSLDRLE 
      KFIFTEWKF HATKTTQLH KSLSKTDQE LFILDITTL 
      VWLDYFKDL AVGARVYLN REPLKNLAG AKTKDNILM 
      ILHLAMQAA LFSLIWYLF ACIFGMKMS SSAFVVPIG 
      YFVFSLL
    CDS sequence
    • >TC015051 RA
      atgtcgagc agcgaaatc gtgtcattt tacaccgga 
      aaaaacttc ttcataact ggggccagc ggtttcgtt 
      ggtatttgt cttttggag aagattctc cgcgtgatt 
      cccaaccat ggtcagatc taccttctc ctgcgcccc 
      aagaagggg aaagccata aacgagcga cttgaagaa 
      atcaaaaag aaccaaatt tttgaaaaa ttactcgct 
      aatcgaagc gtcgaggac gtgtttcgc ggcgtccaa 
      gcaatcgct ggtgacgta ggccaggac aatttgggg 
      atttccccc gaagatcgt cgcctactt gtcgagaat 
      gtcaatgtt ataatccac tcagctgcg actttagat 
      ttcggtgat acgttgaaa actacagta aatattaat 
      cttttgggg acacgtcgc atcacccag ttggctaag 
      gagtgtcga caattgaag gttctgaca catgttagc 
      agcgcctac gtgaattcg tatcgcctg gagtgtgaa 
      gaaatcatc tatgacaag ccatgtgac agcgaggag 
      ctgatctca ttggtggag aagctaagc gaggcggag 
      cttgagaag caaacaccg gaaatactc aaagatcac 
      ccaaacacc tacaccata accaaacat ttggccgaa 
      catgaaata aaaaactgc gaaacgatt tttccctgc 
      actattgta cggcctagc atgattatt ggggcgtgg 
      aaggaaccc atccctgga tggacaatc agtaaaaat 
      gggcctcag ggcttcctc atgggggct gccaaaggg 
      gtgattcgc cgacttccg gtcggtaaa agcctaatt 
      tatgactac atccctgtt gatgtagtt gttaataat 
      ttactggcg gctgggtac agtgctgcc acccataac 
      gccaaacaa gtggaagtg tatcactgc acgtctagc 
      actcgtaac ccgttctct tggatttta atcgaggat 
      cgggttaat agttttttg cacgaatat ccgttaaag 
      tcagccgtt tggtatccg tatttgaag tttttgccc 
      tctgttacg tggtacaaa atttccgcc tttttcgta 
      cacattctg ccagcgata cttctcgat tttgtcact 
      agagtcgct ggggggcgc cctatctta atgaaatta 
      caccgaaac gtcaacacg tcactagat cgtttggaa 
      aaattcatt ttcaccgaa tggaaattc cacgcaact 
      aaaaccaca caactgcac aaatcgtta tcaaaaacg 
      gaccaagaa ttatttatt ttggacata acaacgctg 
      gtttggttg gattatttc aaagatcta gcagtcggg 
      gcccgagtc tacttaaac cgcgaacct ttgaaaaat 
      ctagccggc gccaaaacc aaagataat atcttgatg 
      attctgcat ctagccatg caggcggcg cttttttca 
      ctcatttgg tatttgttt gcgtgcatt ttcggcatg 
      aaaatgtcg tcgtcagcg ttcgttgtc ccgattggt 
      tattttgta ttcagtttg ttgtaa
    mRNA sequence
    • >TC015051 RA
      tgagagtca aaacttgca tgtggaaaa acgagttaa 
      tatacaggt tggagtgtt tttcttgtt tcgtcttcg 
      ttaagtgaa tgtgctacg tgttattat cacaaattt 
      tccgatttt ggttgaaag aatgtcgag cagcgaaat 
      cgtgtcatt ttacaccgg aaaaaactt cttcataac 
      tggggccag cggtttcgt tggtatttg tcttttgga 
      gaagattct ccgcgtgat tcccaacca tggtcagat 
      ctaccttct cctgcgccc caagaaggg gaaagccat 
      aaacgagcg acttgaaga aatcaaaaa gaaccaaat 
      ttttgaaaa attactcgc taatcgaag cgtcgagga 
      cgtgtttcg cggcgtcca agcaatcgc tggtgacgt 
      aggccagga caatttggg gatttcccc cgaagatcg 
      tcgcctact tgtcgagaa tgtcaatgt tataatcca 
      ctcagctgc gactttaga tttcggtga tacgttgaa 
      aactacagt aaatattaa tcttttggg gacacgtcg 
      catcaccca gttggctaa ggagtgtcg acaattgaa 
      ggttctgac acatgttag cagcgccta cgtgaattc 
      gtatcgcct ggagtgtga agaaatcat ctatgacaa 
      gccatgtga cagcgagga gctgatctc attggtgga 
      gaagctaag cgaggcgga gcttgagaa gcaaacacc 
      ggaaatact caaagatca cccaaacac ctacaccat 
      aaccaaaca tttggccga acatgaaat aaaaaactg 
      cgaaacgat ttttccctg cactattgt acggcctag 
      catgattat tggggcgtg gaaggaacc catccctgg 
      atggacaat cagtaaaaa tgggcctca gggcttcct 
      catgggggc tgccaaagg ggtgattcg ccgacttcc 
      ggtcggtaa aagcctaat ttatgacta catccctgt 
      tgatgtagt tgttaataa tttactggc ggctgggta 
      cagtgctgc cacccataa cgccaaaca agtggaagt 
      gtatcactg cacgtctag cactcgtaa cccgttctc 
      ttggatttt aatcgagga tcgggttaa tagtttttt 
      gcacgaata tccgttaaa gtcagccgt ttggtatcc 
      gtatttgaa gtttttgcc ctctgttac gtggtacaa 
      aatttccgc ctttttcgt acacattct gccagcgat 
      acttctcga ttttgtcac tagagtcgc tggggggcg 
      ccctatctt aatgaaatt acaccgaaa cgtcaacac 
      gtcactaga tcgtttgga aaaattcat tttcaccga 
      atggaaatt ccacgcaac taaaaccac acaactgca 
      caaatcgtt atcaaaaac ggaccaaga attatttat 
      tttggacat aacaacgct ggtttggtt ggattattt 
      caaagatct agcagtcgg ggcccgagt ctacttaaa 
      ccgcgaacc tttgaaaaa tctagccgg cgccaaaac 
      caaagataa tatcttgat gattctgca tctagccat 
      gcaggcggc gcttttttc actcatttg gtatttgtt 
      tgcgtgcat tttcggcat gaaaatgtc gtcgtcagc 
      gttcgttgt cccgattgg ttattttgt attcagttt 
      gttgtaatt tttgtatta atttgaata tatggttga 
      aatgaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY