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Gene information
General information
TC015051 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4220848..4225092
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015051
    iBeetle screen iB_02384

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015051 RA
      MSSSEIVSF YTGKNFFIT GASGFVGIC LLEKILRVI 
PNHGQIYLL LRPKKGKAI NERLEEIKK NQIFEKLLA 
NRSVEDVFR GVQAIAGDV GQDNLGISP EDRRLLVEN 
VNVIIHSAA TLDFGDTLK TTVNINLLG TRRITQLAK 
ECRQLKVLT HVSSAYVNS YRLECEEII YDKPCDSEE 
LISLVEKLS EAELEKQTP EILKDHPNT YTITKHLAE 
HEIKNCETI FPCTIVRPS MIIGAWKEP IPGWTISKN 
GPQGFLMGA AKGVIRRLP VGKSLIYDY IPVDVVVNN 
LLAAGYSAA THNAKQVEV YHCTSSTRN PFSWILIED 
RVNSFLHEY PLKSAVWYP YLKFLPSVT WYKISAFFV 
HILPAILLD FVTRVAGGR PILMKLHRN VNTSLDRLE 
KFIFTEWKF HATKTTQLH KSLSKTDQE LFILDITTL 
VWLDYFKDL AVGARVYLN REPLKNLAG AKTKDNILM 
ILHLAMQAA LFSLIWYLF ACIFGMKMS SSAFVVPIG 
YFVFSLL
    CDS sequence
    • >TC015051 RA
      atgtcgagc agcgaaatc gtgtcattt tacaccgga 
aaaaacttc ttcataact ggggccagc ggtttcgtt 
ggtatttgt cttttggag aagattctc cgcgtgatt 
cccaaccat ggtcagatc taccttctc ctgcgcccc 
aagaagggg aaagccata aacgagcga cttgaagaa 
atcaaaaag aaccaaatt tttgaaaaa ttactcgct 
aatcgaagc gtcgaggac gtgtttcgc ggcgtccaa 
gcaatcgct ggtgacgta ggccaggac aatttgggg 
atttccccc gaagatcgt cgcctactt gtcgagaat 
gtcaatgtt ataatccac tcagctgcg actttagat 
ttcggtgat acgttgaaa actacagta aatattaat 
cttttgggg acacgtcgc atcacccag ttggctaag 
gagtgtcga caattgaag gttctgaca catgttagc 
agcgcctac gtgaattcg tatcgcctg gagtgtgaa 
gaaatcatc tatgacaag ccatgtgac agcgaggag 
ctgatctca ttggtggag aagctaagc gaggcggag 
cttgagaag caaacaccg gaaatactc aaagatcac 
ccaaacacc tacaccata accaaacat ttggccgaa 
catgaaata aaaaactgc gaaacgatt tttccctgc 
actattgta cggcctagc atgattatt ggggcgtgg 
aaggaaccc atccctgga tggacaatc agtaaaaat 
gggcctcag ggcttcctc atgggggct gccaaaggg 
gtgattcgc cgacttccg gtcggtaaa agcctaatt 
tatgactac atccctgtt gatgtagtt gttaataat 
ttactggcg gctgggtac agtgctgcc acccataac 
gccaaacaa gtggaagtg tatcactgc acgtctagc 
actcgtaac ccgttctct tggatttta atcgaggat 
cgggttaat agttttttg cacgaatat ccgttaaag 
tcagccgtt tggtatccg tatttgaag tttttgccc 
tctgttacg tggtacaaa atttccgcc tttttcgta 
cacattctg ccagcgata cttctcgat tttgtcact 
agagtcgct ggggggcgc cctatctta atgaaatta 
caccgaaac gtcaacacg tcactagat cgtttggaa 
aaattcatt ttcaccgaa tggaaattc cacgcaact 
aaaaccaca caactgcac aaatcgtta tcaaaaacg 
gaccaagaa ttatttatt ttggacata acaacgctg 
gtttggttg gattatttc aaagatcta gcagtcggg 
gcccgagtc tacttaaac cgcgaacct ttgaaaaat 
ctagccggc gccaaaacc aaagataat atcttgatg 
attctgcat ctagccatg caggcggcg cttttttca 
ctcatttgg tatttgttt gcgtgcatt ttcggcatg 
aaaatgtcg tcgtcagcg ttcgttgtc ccgattggt 
tattttgta ttcagtttg ttgtaa
    mRNA sequence
    • >TC015051 RA
      tgagagtca aaacttgca tgtggaaaa acgagttaa 
tatacaggt tggagtgtt tttcttgtt tcgtcttcg 
ttaagtgaa tgtgctacg tgttattat cacaaattt 
tccgatttt ggttgaaag aatgtcgag cagcgaaat 
cgtgtcatt ttacaccgg aaaaaactt cttcataac 
tggggccag cggtttcgt tggtatttg tcttttgga 
gaagattct ccgcgtgat tcccaacca tggtcagat 
ctaccttct cctgcgccc caagaaggg gaaagccat 
aaacgagcg acttgaaga aatcaaaaa gaaccaaat 
ttttgaaaa attactcgc taatcgaag cgtcgagga 
cgtgtttcg cggcgtcca agcaatcgc tggtgacgt 
aggccagga caatttggg gatttcccc cgaagatcg 
tcgcctact tgtcgagaa tgtcaatgt tataatcca 
ctcagctgc gactttaga tttcggtga tacgttgaa 
aactacagt aaatattaa tcttttggg gacacgtcg 
catcaccca gttggctaa ggagtgtcg acaattgaa 
ggttctgac acatgttag cagcgccta cgtgaattc 
gtatcgcct ggagtgtga agaaatcat ctatgacaa 
gccatgtga cagcgagga gctgatctc attggtgga 
gaagctaag cgaggcgga gcttgagaa gcaaacacc 
ggaaatact caaagatca cccaaacac ctacaccat 
aaccaaaca tttggccga acatgaaat aaaaaactg 
cgaaacgat ttttccctg cactattgt acggcctag 
catgattat tggggcgtg gaaggaacc catccctgg 
atggacaat cagtaaaaa tgggcctca gggcttcct 
catgggggc tgccaaagg ggtgattcg ccgacttcc 
ggtcggtaa aagcctaat ttatgacta catccctgt 
tgatgtagt tgttaataa tttactggc ggctgggta 
cagtgctgc cacccataa cgccaaaca agtggaagt 
gtatcactg cacgtctag cactcgtaa cccgttctc 
ttggatttt aatcgagga tcgggttaa tagtttttt 
gcacgaata tccgttaaa gtcagccgt ttggtatcc 
gtatttgaa gtttttgcc ctctgttac gtggtacaa 
aatttccgc ctttttcgt acacattct gccagcgat 
acttctcga ttttgtcac tagagtcgc tggggggcg 
ccctatctt aatgaaatt acaccgaaa cgtcaacac 
gtcactaga tcgtttgga aaaattcat tttcaccga 
atggaaatt ccacgcaac taaaaccac acaactgca 
caaatcgtt atcaaaaac ggaccaaga attatttat 
tttggacat aacaacgct ggtttggtt ggattattt 
caaagatct agcagtcgg ggcccgagt ctacttaaa 
ccgcgaacc tttgaaaaa tctagccgg cgccaaaac 
caaagataa tatcttgat gattctgca tctagccat 
gcaggcggc gcttttttc actcatttg gtatttgtt 
tgcgtgcat tttcggcat gaaaatgtc gtcgtcagc 
gttcgttgt cccgattgg ttattttgt attcagttt 
gttgtaatt tttgtatta atttgaata tatggttga 
aatgaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY