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Gene information
General information
TC015050 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid41: 547426..549082
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015050
    iBeetle screen iB_02383
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition number 2nd lateral oviduct: 2 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015050 RA
      MFSRVVRPS LASARSFST SKQNNVKVA VAGASGGIG 
      QPLSLLLKQ SPLVTELSL YDIVHTPGV AADLSHIET 
      PAKVKGFNG PENLKKAFE GAEVIIIPA GVPRKPGMT 
      RDDLFNTNA SIVQTLAEA AAESAPKAL IGIISNPVN 
      STVPIAAEV LKKAGKYDP KRLFGVSTL DVVRANTFV 
      AELKGLNPL EVKVPVIGG HSGVTIIPL ISQATPSVT 
      FPPDQLKAL TERIQEAGT EVVKAKAGA GSATLSMAY 
      AGARFAISL IRALKGEQN IIECAYVES NLTEAKYFS 
      TPLLLGKNG LEKNLGLGK LSDFEQDLL KKAIPELKK 
      NIQKGEDFV NKK
    CDS sequence
    • >TC015050 RA
      atgttctcg cgagtggtg cgtccaagc cttgccagt 
      gcccgcagt tttagcacg tccaaacag aacaatgtg 
      aaagtggcc gttgccggc gcctccggg ggcatcggc 
      cagcccctg tcgctcctc ctcaaacag agcccccta 
      gtcaccgaa ctgtccctc tacgatatc gtccatacc 
      ccaggagtc gccgccgac ctgtcccac attgagacc 
      cccgccaaa gtcaaaggc ttcaacggc cccgaaaac 
      ctcaaaaag gccttcgag ggggccgaa gtgatcata 
      atcccggcg ggggttccc cgaaagcct ggcatgact 
      cgcgacgac ttgttcaac acaaacgcc tcgatcgtg 
      caaactctg gccgaagcc gccgccgag agcgccccc 
      aaagcccta atcggcatc atctcgaac cccgtcaac 
      tccaccgtc cccatcgcg gccgaggtt ttgaaaaaa 
      gcggggaag tacgacccc aagcgcctc ttcggggtc 
      tcaaccctc gacgtggtg cgcgccaac accttcgtg 
      gccgagctc aaagggctg aaccccttg gaggtcaaa 
      gtgccagtg attgggggg cactcgggg gttaccata 
      atcccactc atttcgcaa gccacccct tcggtgacc 
      ttcccccca gatcagctc aaagcgtta acggagcgg 
      atccaggag gctggcacg gaagttgtc aaggccaag 
      gcaggagcg ggctcagcc accctgtca atggcgtac 
      gccggggcc cgatttgca atctcccta atccgggcc 
      ctcaagggc gaacaaaac atcatcgag tgtgcctac 
      gttgaatct aacttaacc gaagccaaa tatttctcg 
      actccgttg cttttgggc aaaaacggg ctcgagaag 
      aacttggga ttgggaaaa ttgagcgat tttgagcaa 
      gacttgctc aaaaaggcc atcccagag ttgaagaaa 
      aatatccaa aagggcgaa gattttgtt aataagaag 
      taa
    mRNA sequence
    • >TC015050 RA
      agaggttcg cagtgaaga gagtcaacg aaaccggca 
      cttggtccg gctttgagg ttatgtaag ggccggtaa 
      aacagaaac cttgaaaac gtaaccgcc aattatcaa 
      ttttacgta attttatta cgtaacgtc tgattaatt 
      taatctttc ccaagtcag ttttgataa gatttatgc 
      gtttatcgc ccgattaga ggcatttat caataattt 
      ggcgagtcg gtaaaacag atagctgaa tttttctgg 
      aacgtgaca taaggttgc ggaaaggtc aagaggttt 
      aaattgcga taagcgcag tcttttcgt ctggaaatt 
      gtccccaga agccttaaa aatgttctc gcgagtggt 
      gcgtccaag ccttgccag tgcccgcag ttttagcac 
      gtccaaaca gaacaatgt gaaagtggc cgttgccgg 
      cgcctccgg gggcatcgg ccagcccct gtcgctcct 
      cctcaaaca gagccccct agtcaccga actgtccct 
      ctacgatat cgtccatac cccaggagt cgccgccga 
      cctgtccca cattgagac ccccgccaa agtcaaagg 
      cttcaacgg ccccgaaaa cctcaaaaa ggccttcga 
      gggggccga agtgatcat aatcccggc gggggttcc 
      ccgaaagcc tggcatgac tcgcgacga cttgttcaa 
      cacaaacgc ctcgatcgt gcaaactct ggccgaagc 
      cgccgccga gagcgcccc caaagccct aatcggcat 
      catctcgaa ccccgtcaa ctccaccgt ccccatcgc 
      ggccgaggt tttgaaaaa agcggggaa gtacgaccc 
      caagcgcct cttcggggt ctcaaccct cgacgtggt 
      gcgcgccaa caccttcgt ggccgagct caaagggct 
      gaacccctt ggaggtcaa agtgccagt gattggggg 
      gcactcggg ggttaccat aatcccact catttcgca 
      agccacccc ttcggtgac cttcccccc agatcagct 
      caaagcgtt aacggagcg gatccagga ggctggcac 
      ggaagttgt caaggccaa ggcaggagc gggctcagc 
      caccctgtc aatggcgta cgccggggc ccgatttgc 
      aatctccct aatccgggc cctcaaggg cgaacaaaa 
      catcatcga gtgtgccta cgttgaatc taacttaac 
      cgaagccaa atatttctc gactccgtt gcttttggg 
      caaaaacgg gctcgagaa gaacttggg attgggaaa 
      attgagcga ttttgagca agacttgct caaaaaggc 
      catcccaga gttgaagaa aaatatcca aaagggcga 
      agattttgt taataagaa gtaaggaaa taaaaaaat 
      attcttacc aacttgcgt tttatttaa taaaaaatt 
      ctactattc