| General information | |||
| TC033415
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 4256167..4264802 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033415 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02382 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC033415 RA
MTEERIFEF LLPKHHDYP KLHCEAAET IAKSFTDVI YDTLRLKSA IPANKRLNV LKFIKTLAS APDNHVTVD ESNCATFIK SLEGQFSDT SDDYLNRVE SDLRAQLPK DLSEEVLQF YKRRKQAHE EKIKRLELI KKHDQEALD NDNFLHRVT RSITYYETS ENVIKQLLG NESKNQNSP NTTDFNSPD YRAKQVQFA YGLLHLKHW ETLLKVLER FKKYETLSN TEQNFLCYL STNLSPDCD HLGPILVND FIFVYESIV LTFCTQIIQ LPDAQIPQF FETLPSDKN YQTLKNLST NREFGLKLK NLLFELYSF SYFICKMAS ILKLSKSVA KLSKNGPII QTAKTLRNA STAAEQKQV VLNVPPTNV TTLNSGIRV ATEDWGSHT ATVGIWIDA GSRYENSKN NGVAHFMEH MAFKGTGKR SQTQLEVEI EDMGAHLNA YTSREQTVY YSKCLAKDV PKAIEILGD IVQNAKLGE AEIERERGV ILREMQEIE SNLQEVVFD HLHAIAYQG TPLANTILG PTANIRAIN ANDLRCYLD NHYKASRIV VAGAGGVNH DELVKLCEQ HLTKLNNNY PDEIPILSP CRFTGSEIR VRDDSLPLA HIAIAVEGT GWTDPDTLT LMVASTLLG AWDRSQASA KQNATTLAR ASGEGELCH SYQSFNTCY KDTGLWGIY FVSDPLKIE DMVFNIQQE FMRLATSVT EGEVERAKA LLTANTLLQ LDTSTAVCE DIGRQLLCY GRRLPPHEL THRINSITA QNVRDVCYK YLYDRCPAI AAVGPVEQL PDYNRIRSS MYWLRV
- >TC033415 RA
atgacagaa gagagaatt tttgaattt ttgctccca aaacaccac gattacccc aaactccac tgcgaagca gccgaaacc attgccaag agcttcacg gatgttatt tacgacact ttgaggctc aaatcggcg attccggcc aataaacgc ctcaacgtt cttaaattc attaaaaca cttgcgtcc gctccagac aatcatgta acagtggac gaaagcaat tgtgcaacg tttattaaa tcacttgag gggcaattc agtgacaca agtgatgat tacttaaac cgggttgag agcgattta agggcacaa ttaccaaaa gatttgtcc gaagaagtg ttacagttt tacaagaga cgcaaacaa gcccacgag gagaaaatc aaacggctt gaactgatc aaaaaacac gaccaggaa gcgctagac aacgacaat tttttgcac agagtcaca cgaagcatc acgtattac gaaacgagc gaaaacgtt attaaacaa ttattggga aacgaatca aagaaccaa aattcgccc aacacaacc gacttcaat tcacccgac tatagggca aaacaagtg caatttgca tacgggtta ctgcacctg aaacactgg gagacctta ctgaaggtt ttagaacgg ttcaaaaaa tacgagact ttgtcaaac accgaacaa aacttcttg tgttacttg agcacgaat ttaagcccc gactgtgac cacttaggg ccgatttta gtcaacgat ttcattttc gtgtacgag agcattgtc ttgaccttt tgtacacaa ataattcaa ctaccagac gcccaaata ccccaattt tttgaaacc ttgccaagt gacaaaaat taccaaacc cttaaaaat ttaagcaca aatcgcgaa tttgggcta aaattgaag aatttattg ttcgagttg tacagtttt agttatttc atttgcaag atggcgtcg attctaaaa ttgtccaaa tccgtggca aaattatca aaaaatggg ccaataatt cagacggct aaaaccctc cgaaatgcc tcaacggcc gctgaacaa aaacaggtc gtcttgaac gtgcctccc acaaacgtg acgacacta aattcaggt ataagggtg gcgactgag gactggggc tcgcacaca gccacggta ggtatctgg atcgacgcc ggcagccgc tatgagaac tcgaaaaac aacggagtc gcccatttc atggaacac atggctttt aaggggacg ggtaagagg agccagacc cagctcgag gtcgagatc gaggacatg ggggcccac ttgaacgcc tacacgagc cgcgaacag actgtttac tattcgaaa tgtttggcc aaagacgtg cccaaagcc attgagatt ttgggcgat attgtgcaa aatgccaaa ctgggcgaa gcggaaatc gagcgcgaa cgcggggtt attttgcgt gaaatgcaa gagattgag tctaatctc caggaggtt gttttcgat catttgcac gcgattgcg tatcaaggg acgcctttg gctaacacg attctgggg cccaccgcc aacattagg gctatcaat gccaatgat ttgcgctgc tatttggat aatcactac aaggcgagc cggattgtg gtggccggt gctgggggt gttaatcac gatgagttg gtcaaactg tgtgagcaa catctgacc aaattgaat aacaactat cctgacgaa atcccgatt ttgagtcct tgccgtttc accggctct gagattcgc gtccgtgac gattccctg cctttggcc cacatcgcc atagctgtg gaaggtacc ggatggacc gacccagac accttgacc ttgatggtg gcttccacc ttgttgggt gcatgggat aggtcccaa gcgtcggca aaacagaac gctacaact ctggcacgt gccagtggc gaaggcgag ttgtgtcac tcgtaccaa agtttcaac acgtgctat aaagatacg ggtttgtgg ggcatttac ttcgtctca gacccgctc aaaatcgaa gatatggtc tttaatatc caacaggag tttatgagg ttggctacg agtgttact gagggtgaa gtcgagagg gctaaggct ctcctcacg gccaatact ttgctccaa ttggatact tccactgct gtgtgtgag gatattggg cgccagttg ttgtgttat ggaaggagg ctgccccct catgaattg acccatagg attaattcc ataacagcg caaaatgta cgagatgtg tgctacaaa tatttgtac gatcggtgt cccgctatt gccgcagtg gggccagtt gagcaactg ccagactac aacaggatc cgtagctca atgtattgg ttacgtgtc taa
- >TC033415 RA
gccataaag tacttcttt atgtcgcaa gtaagcgct tgacatttc aaacgtcaa aatgacaga agagagaat ttttgaatt tttgctccc aaaacacca cgattaccc caaactcca ctgcgaagc agccgaaac cattgccaa gagcttcac ggatgttat ttacgacac tttgaggct caaatcggc gattccggc caataaacg cctcaacgt tcttaaatt cattaaaac acttgcgtc cgctccaga caatcatgt aacagtgga cgaaagcaa ttgtgcaac gtttattaa atcacttga ggggcaatt cagtgacac aagtgatga ttacttaaa ccgggttga gagcgattt aagggcaca attaccaaa agatttgtc cgaagaagt gttacagtt ttacaagag acgcaaaca agcccacga ggagaaaat caaacggct tgaactgat caaaaaaca cgaccagga agcgctaga caacgacaa ttttttgca cagagtcac acgaagcat cacgtatta cgaaacgag cgaaaacgt tattaaaca attattggg aaacgaatc aaagaacca aaattcgcc caacacaac cgacttcaa ttcacccga ctatagggc aaaacaagt gcaatttgc atacgggtt actgcacct gaaacactg ggagacctt actgaaggt tttagaacg gttcaaaaa atacgagac tttgtcaaa caccgaaca aaacttctt gtgttactt gagcacgaa tttaagccc cgactgtga ccacttagg gccgatttt agtcaacga tttcatttt cgtgtacga gagcattgt cttgacctt ttgtacaca aataattca actaccaga cgcccaaat accccaatt ttttgaaac cttgccaag tgacaaaaa ttaccaaac ccttaaaaa tttaagcac aaatcgcga atttgggct aaaattgaa gaatttatt gttcgagtt gtacagttt tagttattt catttgcaa gatggcgtc gattctaaa attgtccaa atccgtggc aaaattatc aaaaaatgg gccaataat tcagacggc taaaaccct ccgaaatgc ctcaacggc cgctgaaca aaaacaggt cgtcttgaa cgtgcctcc cacaaacgt gacgacact aaattcagg tataagggt ggcgactga ggactgggg ctcgcacac agccacggt aggtatctg gatcgacgc cggcagccg ctatgagaa ctcgaaaaa caacggagt cgcccattt catggaaca catggcttt taaggggac gggtaagag gagccagac ccagctcga ggtcgagat cgaggacat gggggccca cttgaacgc ctacacgag ccgcgaaca gactgttta ctattcgaa atgtttggc caaagacgt gcccaaagc cattgagat tttgggcga tattgtgca aaatgccaa actgggcga agcggaaat cgagcgcga acgcggggt tattttgcg tgaaatgca agagattga gtctaatct ccaggaggt tgttttcga tcatttgca cgcgattgc gtatcaagg gacgccttt ggctaacac gattctggg gcccaccgc caacattag ggctatcaa tgccaatga tttgcgctg ctatttgga taatcacta caaggcgag ccggattgt ggtggccgg tgctggggg tgttaatca cgatgagtt ggtcaaact gtgtgagca acatctgac caaattgaa taacaacta tcctgacga aatcccgat tttgagtcc ttgccgttt caccggctc tgagattcg cgtccgtga cgattccct gcctttggc ccacatcgc catagctgt ggaaggtac cggatggac cgacccaga caccttgac cttgatggt ggcttccac cttgttggg tgcatggga taggtccca agcgtcggc aaaacagaa cgctacaac tctggcacg tgccagtgg cgaaggcga gttgtgtca ctcgtacca aagtttcaa cacgtgcta taaagatac gggtttgtg gggcattta cttcgtctc agacccgct caaaatcga agatatggt ctttaatat ccaacagga gtttatgag gttggctac gagtgttac tgagggtga agtcgagag ggctaaggc tctcctcac ggccaatac tttgctcca attggatac ttccactgc tgtgtgtga ggatattgg gcgccagtt gttgtgtta tggaaggag gctgccccc tcatgaatt gacccatag gattaattc cataacagc gcaaaatgt acgagatgt gtgctacaa atatttgta cgatcggtg tcccgctat tgccgcagt ggggccagt tgagcaact gccagacta caacaggat ccgtagctc aatgtattg gttacgtgt ctaagtgtt taaaaaata gtctttaaa ttcaacaat gtaaataat tttttgttc caacttggt taattttat taataaagg tacgtgtca cag
