Status
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Gene information
General information
TC033415
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015046,TC015045 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4256167..4264802
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033415
    iBeetle screen iB_02382

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033415 RA
      MTEERIFEF LLPKHHDYP KLHCEAAET IAKSFTDVI 
YDTLRLKSA IPANKRLNV LKFIKTLAS APDNHVTVD 
ESNCATFIK SLEGQFSDT SDDYLNRVE SDLRAQLPK 
DLSEEVLQF YKRRKQAHE EKIKRLELI KKHDQEALD 
NDNFLHRVT RSITYYETS ENVIKQLLG NESKNQNSP 
NTTDFNSPD YRAKQVQFA YGLLHLKHW ETLLKVLER 
FKKYETLSN TEQNFLCYL STNLSPDCD HLGPILVND 
FIFVYESIV LTFCTQIIQ LPDAQIPQF FETLPSDKN 
YQTLKNLST NREFGLKLK NLLFELYSF SYFICKMAS 
ILKLSKSVA KLSKNGPII QTAKTLRNA STAAEQKQV 
VLNVPPTNV TTLNSGIRV ATEDWGSHT ATVGIWIDA 
GSRYENSKN NGVAHFMEH MAFKGTGKR SQTQLEVEI 
EDMGAHLNA YTSREQTVY YSKCLAKDV PKAIEILGD 
IVQNAKLGE AEIERERGV ILREMQEIE SNLQEVVFD 
HLHAIAYQG TPLANTILG PTANIRAIN ANDLRCYLD 
NHYKASRIV VAGAGGVNH DELVKLCEQ HLTKLNNNY 
PDEIPILSP CRFTGSEIR VRDDSLPLA HIAIAVEGT 
GWTDPDTLT LMVASTLLG AWDRSQASA KQNATTLAR 
ASGEGELCH SYQSFNTCY KDTGLWGIY FVSDPLKIE 
DMVFNIQQE FMRLATSVT EGEVERAKA LLTANTLLQ 
LDTSTAVCE DIGRQLLCY GRRLPPHEL THRINSITA 
QNVRDVCYK YLYDRCPAI AAVGPVEQL PDYNRIRSS 
MYWLRV
    CDS sequence
    • >TC033415 RA
      atgacagaa gagagaatt tttgaattt ttgctccca 
aaacaccac gattacccc aaactccac tgcgaagca 
gccgaaacc attgccaag agcttcacg gatgttatt 
tacgacact ttgaggctc aaatcggcg attccggcc 
aataaacgc ctcaacgtt cttaaattc attaaaaca 
cttgcgtcc gctccagac aatcatgta acagtggac 
gaaagcaat tgtgcaacg tttattaaa tcacttgag 
gggcaattc agtgacaca agtgatgat tacttaaac 
cgggttgag agcgattta agggcacaa ttaccaaaa 
gatttgtcc gaagaagtg ttacagttt tacaagaga 
cgcaaacaa gcccacgag gagaaaatc aaacggctt 
gaactgatc aaaaaacac gaccaggaa gcgctagac 
aacgacaat tttttgcac agagtcaca cgaagcatc 
acgtattac gaaacgagc gaaaacgtt attaaacaa 
ttattggga aacgaatca aagaaccaa aattcgccc 
aacacaacc gacttcaat tcacccgac tatagggca 
aaacaagtg caatttgca tacgggtta ctgcacctg 
aaacactgg gagacctta ctgaaggtt ttagaacgg 
ttcaaaaaa tacgagact ttgtcaaac accgaacaa 
aacttcttg tgttacttg agcacgaat ttaagcccc 
gactgtgac cacttaggg ccgatttta gtcaacgat 
ttcattttc gtgtacgag agcattgtc ttgaccttt 
tgtacacaa ataattcaa ctaccagac gcccaaata 
ccccaattt tttgaaacc ttgccaagt gacaaaaat 
taccaaacc cttaaaaat ttaagcaca aatcgcgaa 
tttgggcta aaattgaag aatttattg ttcgagttg 
tacagtttt agttatttc atttgcaag atggcgtcg 
attctaaaa ttgtccaaa tccgtggca aaattatca 
aaaaatggg ccaataatt cagacggct aaaaccctc 
cgaaatgcc tcaacggcc gctgaacaa aaacaggtc 
gtcttgaac gtgcctccc acaaacgtg acgacacta 
aattcaggt ataagggtg gcgactgag gactggggc 
tcgcacaca gccacggta ggtatctgg atcgacgcc 
ggcagccgc tatgagaac tcgaaaaac aacggagtc 
gcccatttc atggaacac atggctttt aaggggacg 
ggtaagagg agccagacc cagctcgag gtcgagatc 
gaggacatg ggggcccac ttgaacgcc tacacgagc 
cgcgaacag actgtttac tattcgaaa tgtttggcc 
aaagacgtg cccaaagcc attgagatt ttgggcgat 
attgtgcaa aatgccaaa ctgggcgaa gcggaaatc 
gagcgcgaa cgcggggtt attttgcgt gaaatgcaa 
gagattgag tctaatctc caggaggtt gttttcgat 
catttgcac gcgattgcg tatcaaggg acgcctttg 
gctaacacg attctgggg cccaccgcc aacattagg 
gctatcaat gccaatgat ttgcgctgc tatttggat 
aatcactac aaggcgagc cggattgtg gtggccggt 
gctgggggt gttaatcac gatgagttg gtcaaactg 
tgtgagcaa catctgacc aaattgaat aacaactat 
cctgacgaa atcccgatt ttgagtcct tgccgtttc 
accggctct gagattcgc gtccgtgac gattccctg 
cctttggcc cacatcgcc atagctgtg gaaggtacc 
ggatggacc gacccagac accttgacc ttgatggtg 
gcttccacc ttgttgggt gcatgggat aggtcccaa 
gcgtcggca aaacagaac gctacaact ctggcacgt 
gccagtggc gaaggcgag ttgtgtcac tcgtaccaa 
agtttcaac acgtgctat aaagatacg ggtttgtgg 
ggcatttac ttcgtctca gacccgctc aaaatcgaa 
gatatggtc tttaatatc caacaggag tttatgagg 
ttggctacg agtgttact gagggtgaa gtcgagagg 
gctaaggct ctcctcacg gccaatact ttgctccaa 
ttggatact tccactgct gtgtgtgag gatattggg 
cgccagttg ttgtgttat ggaaggagg ctgccccct 
catgaattg acccatagg attaattcc ataacagcg 
caaaatgta cgagatgtg tgctacaaa tatttgtac 
gatcggtgt cccgctatt gccgcagtg gggccagtt 
gagcaactg ccagactac aacaggatc cgtagctca 
atgtattgg ttacgtgtc taa
    mRNA sequence
    • >TC033415 RA
      gccataaag tacttcttt atgtcgcaa gtaagcgct 
tgacatttc aaacgtcaa aatgacaga agagagaat 
ttttgaatt tttgctccc aaaacacca cgattaccc 
caaactcca ctgcgaagc agccgaaac cattgccaa 
gagcttcac ggatgttat ttacgacac tttgaggct 
caaatcggc gattccggc caataaacg cctcaacgt 
tcttaaatt cattaaaac acttgcgtc cgctccaga 
caatcatgt aacagtgga cgaaagcaa ttgtgcaac 
gtttattaa atcacttga ggggcaatt cagtgacac 
aagtgatga ttacttaaa ccgggttga gagcgattt 
aagggcaca attaccaaa agatttgtc cgaagaagt 
gttacagtt ttacaagag acgcaaaca agcccacga 
ggagaaaat caaacggct tgaactgat caaaaaaca 
cgaccagga agcgctaga caacgacaa ttttttgca 
cagagtcac acgaagcat cacgtatta cgaaacgag 
cgaaaacgt tattaaaca attattggg aaacgaatc 
aaagaacca aaattcgcc caacacaac cgacttcaa 
ttcacccga ctatagggc aaaacaagt gcaatttgc 
atacgggtt actgcacct gaaacactg ggagacctt 
actgaaggt tttagaacg gttcaaaaa atacgagac 
tttgtcaaa caccgaaca aaacttctt gtgttactt 
gagcacgaa tttaagccc cgactgtga ccacttagg 
gccgatttt agtcaacga tttcatttt cgtgtacga 
gagcattgt cttgacctt ttgtacaca aataattca 
actaccaga cgcccaaat accccaatt ttttgaaac 
cttgccaag tgacaaaaa ttaccaaac ccttaaaaa 
tttaagcac aaatcgcga atttgggct aaaattgaa 
gaatttatt gttcgagtt gtacagttt tagttattt 
catttgcaa gatggcgtc gattctaaa attgtccaa 
atccgtggc aaaattatc aaaaaatgg gccaataat 
tcagacggc taaaaccct ccgaaatgc ctcaacggc 
cgctgaaca aaaacaggt cgtcttgaa cgtgcctcc 
cacaaacgt gacgacact aaattcagg tataagggt 
ggcgactga ggactgggg ctcgcacac agccacggt 
aggtatctg gatcgacgc cggcagccg ctatgagaa 
ctcgaaaaa caacggagt cgcccattt catggaaca 
catggcttt taaggggac gggtaagag gagccagac 
ccagctcga ggtcgagat cgaggacat gggggccca 
cttgaacgc ctacacgag ccgcgaaca gactgttta 
ctattcgaa atgtttggc caaagacgt gcccaaagc 
cattgagat tttgggcga tattgtgca aaatgccaa 
actgggcga agcggaaat cgagcgcga acgcggggt 
tattttgcg tgaaatgca agagattga gtctaatct 
ccaggaggt tgttttcga tcatttgca cgcgattgc 
gtatcaagg gacgccttt ggctaacac gattctggg 
gcccaccgc caacattag ggctatcaa tgccaatga 
tttgcgctg ctatttgga taatcacta caaggcgag 
ccggattgt ggtggccgg tgctggggg tgttaatca 
cgatgagtt ggtcaaact gtgtgagca acatctgac 
caaattgaa taacaacta tcctgacga aatcccgat 
tttgagtcc ttgccgttt caccggctc tgagattcg 
cgtccgtga cgattccct gcctttggc ccacatcgc 
catagctgt ggaaggtac cggatggac cgacccaga 
caccttgac cttgatggt ggcttccac cttgttggg 
tgcatggga taggtccca agcgtcggc aaaacagaa 
cgctacaac tctggcacg tgccagtgg cgaaggcga 
gttgtgtca ctcgtacca aagtttcaa cacgtgcta 
taaagatac gggtttgtg gggcattta cttcgtctc 
agacccgct caaaatcga agatatggt ctttaatat 
ccaacagga gtttatgag gttggctac gagtgttac 
tgagggtga agtcgagag ggctaaggc tctcctcac 
ggccaatac tttgctcca attggatac ttccactgc 
tgtgtgtga ggatattgg gcgccagtt gttgtgtta 
tggaaggag gctgccccc tcatgaatt gacccatag 
gattaattc cataacagc gcaaaatgt acgagatgt 
gtgctacaa atatttgta cgatcggtg tcccgctat 
tgccgcagt ggggccagt tgagcaact gccagacta 
caacaggat ccgtagctc aatgtattg gttacgtgt 
ctaagtgtt taaaaaata gtctttaaa ttcaacaat 
gtaaataat tttttgttc caacttggt taattttat 
taataaagg tacgtgtca cag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY