Status
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Gene information
General information
TC015014 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5017339..5042764
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015014
    iBeetle screen iB_02377

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015014 RA
      MTQQLLPIK FQEHLQLTN VGINVANIS FATLTMESD 
      KFICVREKV GDTSQVVII DMGDTANPI RRPITAESA 
      IMNPASKVI ALKGKAGVE AQKTLQIFN IEMKSKMKA 
      HTMSEDVIF WKWISLNTL ALVTETSVY HWSMEGDST 
      PVKMFDRHS SLNGCQIIN YRTDPKQNW LLLVGISAQ 
      QSRVVGAMQ LYSVERKCS QPIEGHAAS FATFKMEGN 
      PEPSTLFCF AVRTVQGGK LHIIEVGQS PAGNQPFPK 
      KTVDVFFPP EAQNDFPVA MQVSAKYDV IYLITKYGY 
      IHMYDIESA ICIYMNRIS SETIFVTAP HESTGGIIG 
      VNRRGQVLS VSVDEDSII RYVNQVLHN PDLALRIAT 
      RNNLAGAEE LFVNKFQML FTNGQYAEA AKVAANAPK 
      GILRTPATI QMFQQVPTQ PGQNSPLLQ YFGILLDQG 
      QLNRYESLE LCKPVLLQG RKQLLEKWL KEDKLECSE 
      ELGDLVKQA DSTLALSVY LRANVPAKV IQSFAETGQ 
      FQKIVLYAK KVNYTPDYI YLLRSVMRT NPDQGAAFA 
      SMLVADEEP LADINQIVD IFMEQNMVQ QCTAFLLDA 
      LKHNRPTEG HLQTRLLEM NLMSAPQVA DAILGNNMF 
      THYDRAHIA QLCEKAGLL QRALEHYTD LYDIKRAVV 
      HTHLLPMDW LVNFFGTLS VEDSLECLK AMLTANIRQ 
      NLQICVQIA TKYHEQLTT KALIDLFES FKSYEGLFY 
      FLGSIVNFS QDPDVHFKY IQAACKTGQ IKEVERICR 
      ESNCYNPES VKNFLKEAK LTDQLPLII VCDRFDFVH 
      DLVLYLYRN SLQKYIEIY VQKVNPSRL PVVVGGLLD 
      VDCAEDIIK NLILVVRGQ FSTDELVEE VEKRNRLKL 
      LLPWLESRV HEGCVEPAT HNALAKIYI DSNNNAERF 
      LKENQWYDS RVVGRYCEK RDPHLACVA YERGQCDRE 
      LIAVCNENS LFKSEARYL VRRRDPELW AEVLQESNP 
      YRRQLIDQV VQTALSETQ DPEDISVTV KAFMTADLP 
      NELIELLEK IVLDSSVFS DHRNLQNLL ILTAIKADA 
      TRVMDYINR LDNYDAPDI ANIAINNHL YEEAFAIFK 
      KFDVNTSAI QVLIEQVNN LDRAYEFAE RCNEPAVWS 
      QLAKAQLNQ GLVKEAIDS YIKADDPSA YMAVVETAS 
      KNNSWEDLV RYLQMARKK SRESYIESE LIYSYAKTG 
      RLADLEEFI SGPNHADIQ KIGDRCFDD KMYDAAKLL 
      YNNVSNFAR LAITLVHLK EFQGAVDSA RKANSTRTW 
      KEVCFACVD AEEFRLAQM CGMHIVVHA DELQDLINY 
      YQDRGYFEE LIGLLEAAL GLERAHMGM FTELAILYS 
      KYKPAKMRE HLELFWSRV NIPKVLRAA EQAHLWAEL 
      VFLYDKYEE YDNAVLAMM AHPTEAWRE GHFKDIITK 
      VANIELYYK AIQFYLDYK PLLLNDLLL VLAPRMDHT 
      RAVAFFTKT GHLQLVKSY LRSVQNLNN KAINEALNS 
      LLIEEEDFQ GLRTSIDAF DNFDNIGLA QKLEKHELT 
      EFRRIAAYL YKGNNRWKQ SVELCKKDR LFRDAMEYT 
      AESKNQELA EELLAWFLE RKAYDCFAA CLYQCYDLL 
      RPDVILELA WRHKIMDLA MPYLIQVTR ELTTKVEKL 
      EQSDAQRQS EAAEETHKP MMINEPQLM LTAGPGMGI 
      PPQAYVPPQ AYAQPGYAP QMPYGAYPG M
    CDS sequence
    • >TC015014 RA
      atgacgcaa caattactc ccgataaag ttccaggag 
      cacttgcag ctcacaaat gtcgggatt aacgtggcc 
      aacatctcc ttcgccact ctcaccatg gagagcgac 
      aagttcatc tgcgtgcgg gagaaggtc ggggacacg 
      tcccaggtg gtcatcatc gacatgggg gacaccgcc 
      aaccccatc agacgcccc atcaccgcc gagagcgcg 
      atcatgaac cccgcctcc aaagtgata gccctcaag 
      ggcaaagcg ggcgtcgag gcccaaaag accttgcag 
      attttcaac atcgagatg aagtccaaa atgaaagca 
      cacaccatg tcagaggac gtcatcttc tggaagtgg 
      atctcgcta aacaccttg gctttggtc accgaaacc 
      tcggtgtat cattggtcc atggagggt gactctaca 
      cctgttaaa atgtttgat cgtcactca tctctgaat 
      ggctgccaa attataaac tatcgcact gatcccaaa 
      caaaactgg ttactattg gtgggcatt agtgcccaa 
      cagtctcgt gttgtgggc gcaatgcaa ctttattcc 
      gtcgaaaga aaatgttca caaccgatc gaaggtcat 
      gctgcctct tttgccaca ttcaaaatg gaaggcaat 
      cctgaacca tcgacactt ttctgcttt gccgtaaga 
      acagtacaa ggaggcaaa ctccacatc atcgaagtg 
      gggcaaagt ccggccggc aaccaacca ttccccaag 
      aaaaccgtc gacgtgttc ttcccccct gaggcccaa 
      aacgacttt cccgtagcg atgcaagtc tcggcaaaa 
      tacgacgtc atctactta atcacaaaa tacggctac 
      attcacatg tacgacatc gaaagcgcc atctgtatc 
      tacatgaat cgtatctca agcgagaca attttcgta 
      acagctccg cacgaatcc accggtggt attatcggt 
      gtgaatcgt cgcggacaa gtattgtct gtttctgtc 
      gacgaagac agtattatt cgatatgtg aatcaagtt 
      ttgcacaat cctgatctg gctttacgt atcgccact 
      agaaataat ctcgcaggc gctgaagag ctgtttgtg 
      aacaagttc caaatgttg ttcacgaat ggacagtat 
      gctgaagcg gccaaagtg gcagccaat gcgccgaaa 
      gggattttg agaacacca gctacgatt caaatgttc 
      caacaagtg cccacgcag ccgggacag aacagtcca 
      ctgttgcag tatttcggg attttgtta gatcaaggg 
      cagctaaac agatacgaa tcgctggaa ttgtgcaaa 
      ccagtcctc ttgcaaggc cgtaaacaa cttctcgaa 
      aaatggcta aaagaagac aaactcgaa tgttcggaa 
      gaattgggc gatctcgta aaacaagcc gactcaact 
      ctcgccctt tctgtctat ctccgagct aacgtcccc 
      gccaaagtc attcaaagt tttgccgaa accggtcag 
      ttccaaaaa atcgtcctc tacgccaaa aaagtcaac 
      tacacaccc gactacatt tacttgctg cgctccgtc 
      atgcggaca aaccccgac cagggggcg gctttcgca 
      agcatgctg gttgccgac gaggaacca ctagccgac 
      atcaaccaa attgtcgat attttcatg gaacaaaac 
      atggtgcaa cagtgcacg gccttcttg ctggacgct 
      ttgaagcac aataggccg accgaaggc cacctccag 
      acgcgcctc ctggagatg aacttaatg tcggcgccg 
      caggtcgcc gacgcaatc ctgggcaac aacatgttt 
      acgcattat gacagagcc catattgcg cagctttgc 
      gagaaagcc gggcttttg cagagggcg ctggagcac 
      tacaccgac ttgtacgac atcaagcgg gctgtggtc 
      cacacccac ttgctgcca atggattgg ctggtgaat 
      ttcttcggg actttgagc gttgaggac agtttggag 
      tgtctcaag gctatgttg acggccaat ataaggcaa 
      aacttgcag atttgtgta caaattgcg accaaatat 
      cacgaacaa cttacaacg aaagcgctg attgatttg 
      tttgagagt tttaagagt tatgaaggg cttttctat 
      tttctgggt tcgattgtg aatttttca caggatccg 
      gatgtgcat ttcaagtat attcaggcg gcgtgtaag 
      accggacaa atcaaggaa gttgaacgg atttgtcga 
      gagtcgaat tgttataat ccggagagt gtgaagaat 
      ttcctcaag gaagcgaaa ctgaccgat caattgccg 
      ttgattatt gtgtgcgat cgcttcgat ttcgtgcac 
      gatttggtg ctgtatttg tacaggaat tcgctgcaa 
      aagtacatc gagatctac gtacaaaag gtaaatccc 
      agccgtctc cccgtggtc gtcggcggc ctcctggac 
      gtagactgc gccgaagac atcatcaaa aacctgatc 
      ctcgtcgtc cgtggccaa ttctccacc gacgaactg 
      gtcgaagaa gtggagaaa cgcaaccgc ttgaaactc 
      ctcctacca tggctggag agtcgcgtc cacgagggc 
      tgtgtcgaa ccggccacc cacaacgcc ctggccaaa 
      atctacatc gattcaaac aacaacgcc gaacgtttc 
      ctcaaagaa aaccaatgg tacgactca cgcgtcgtt 
      ggccgctac tgcgaaaaa cgcgacccc catctcgcc 
      tgcgtcgcc tacgagcgc ggccaatgc gaccgcgaa 
      ctaatcgca gtctgtaac gaaaactcg cttttcaaa 
      tccgaggcg agatattta gtcagacgg cgggacccg 
      gaattatgg gccgaagtc ttgcaggaa agtaacccg 
      tacaggaga caattgatc gatcaagtg gtccagaca 
      gcgctgagc gaaacgcaa gacccggag gacatctcc 
      gtgactgtc aaagccttc atgactgct gaccttcca 
      aatgaattg atcgagttg cttgagaaa atcgtcctg 
      gacagttcg gttttctcc gatcaccga aacttgcaa 
      aacctcctg atcctaacg gcgatcaaa gccgatgcc 
      acccgagtc atggactac atcaaccgt ctagataat 
      tacgacgct cctgatatc gcaaatatc gcaataaat 
      aaccattta tacgaggag gcgtttgca attttcaag 
      aaattcgac gttaacact tcagccatt caggttctg 
      atcgagcag gtgaacaat ttggatcgt gcgtatgag 
      tttgcggag cgttgtaat gagccggcg gtttggagt 
      cagttggcc aaggcacag ttgaatcaa gggttggtc 
      aaggaggcg atcgattcg tacattaag gccgatgat 
      ccgtcggcc tatatggcc gtggtggag acggcgtcc 
      aagaataac tcgtgggag gatttggtg cggtatttg 
      caaatggcg aggaagaag tcacgggag agttatatc 
      gagtcggag ttgatttat tcgtatgcc aagacggga 
      cggcttgcc gatttggag gagtttata agtgggccg 
      aatcatgcc gatatacag aagatcggc gatcgatgt 
      tttgatgat aaaatgtac gatgcggcc aagttgttg 
      tataataat gtctcgaat tttgcgagg ttggcgatt 
      actttggtg catttgaag gagtttcag ggagctgtc 
      gacagcgcc cgcaaggcc aacagcacc cgcacctgg 
      aaagaggta tgttttgcg tgcgtggac gccgaagag 
      ttccgtttg gcgcaaatg tgcggaatg cacattgtc 
      gtccacgcc gacgaactg caagatctc atcaactac 
      taccaagac cgcggctat ttcgaagag ctcatcggt 
      ctgctggag gccgcccta ggtctcgag cgcgcccac 
      atgggcatg ttcaccgag ctggccatc ctctattcg 
      aaatacaag ccggcgaaa atgcgcgaa cacctggag 
      ctgttctgg tcgcgcgtc aacattccc aaagtattg 
      cgcgccgcc gaacaggcg catctctgg gccgaactg 
      gtctttctc tacgataag tacgaggag tacgataat 
      gccgttttg gccatgatg gcgcatccg acggaggca 
      tggcgtgaa ggacacttt aaagatatt atcactaaa 
      gtggccaat attgagttg tactacaag gcgattcag 
      ttttatttg gattataag ccgttgttg ttgaacgat 
      ttgttgctg gttttggca ccgagaatg gatcatacg 
      agggctgtg gcgtttttc acaaagacg ggacattta 
      caacttgtc aagtcttat ttgaggtcg gtgcagaat 
      ttgaataat aaggccatt aatgaggcg ctaaattcg 
      ctattgatt gaagaagag gatttccag ggtttgaga 
      acatcgatc gacgcattc gacaatttc gacaatatc 
      ggcttggcc caaaaactc gaaaaacac gaactgacc 
      gaattcaga cgcatcgcc gcctattta tacaaaggc 
      aacaacaga tggaaacag agcgtggaa ttatgtaag 
      aaggatcgc cttttcaga gatgcgatg gaatacaca 
      gctgaatcc aaaaatcaa gaattggcc gaagaacta 
      ctggcgtgg tttttggaa cgaaaggcc tatgattgt 
      tttgcagca tgtctctac caatgctat gatttatta 
      aggcctgac gtaatattg gaactagcc tggagacat 
      aaaatcatg gatttggcg atgccttat ttgattcaa 
      gtaacgaga gaattaacc acaaaagtg gaaaaattg 
      gaacaatcg gatgcacaa aggcagagc gaggccgcc 
      gaagaaaca cacaagccg atgatgatc aacgagcca 
      cagctaatg ctaacggca ggacctggc atgggtatt 
      ccaccgcag gcgtacgtc cccccgcag gcctacgcc 
      cagccaggc tacgccccc cagatgccg tacggcgct 
      tacccgggc atgtaa
    mRNA sequence
    • >TC015014 RA
      gtctacaag caactcggc gccatcttg ggtgtcgca 
      gtttgacta tctgtcagt gtcagtgta gctttgtaa 
      ttagtcatt ctgcgaaaa aaacggtga aaattcaga 
      aaaagactg aagagtgtt ttaagttta gttccgtgc 
      ttgctgttg taaaaattt cggcagcat gacgcaaca 
      attactccc gataaagtt ccaggagca cttgcagct 
      cacaaatgt cgggattaa cgtggccaa catctcctt 
      cgccactct caccatgga gagcgacaa gttcatctg 
      cgtgcggga gaaggtcgg ggacacgtc ccaggtggt 
      catcatcga catggggga caccgccaa ccccatcag 
      acgccccat caccgccga gagcgcgat catgaaccc 
      cgcctccaa agtgatagc cctcaaggg caaagcggg 
      cgtcgaggc ccaaaagac cttgcagat tttcaacat 
      cgagatgaa gtccaaaat gaaagcaca caccatgtc 
      agaggacgt catcttctg gaagtggat ctcgctaaa 
      caccttggc tttggtcac cgaaacctc ggtgtatca 
      ttggtccat ggagggtga ctctacacc tgttaaaat 
      gtttgatcg tcactcatc tctgaatgg ctgccaaat 
      tataaacta tcgcactga tcccaaaca aaactggtt 
      actattggt gggcattag tgcccaaca gtctcgtgt 
      tgtgggcgc aatgcaact ttattccgt cgaaagaaa 
      atgttcaca accgatcga aggtcatgc tgcctcttt 
      tgccacatt caaaatgga aggcaatcc tgaaccatc 
      gacactttt ctgctttgc cgtaagaac agtacaagg 
      aggcaaact ccacatcat cgaagtggg gcaaagtcc 
      ggccggcaa ccaaccatt ccccaagaa aaccgtcga 
      cgtgttctt cccccctga ggcccaaaa cgactttcc 
      cgtagcgat gcaagtctc ggcaaaata cgacgtcat 
      ctacttaat cacaaaata cggctacat tcacatgta 
      cgacatcga aagcgccat ctgtatcta catgaatcg 
      tatctcaag cgagacaat tttcgtaac agctccgca 
      cgaatccac cggtggtat tatcggtgt gaatcgtcg 
      cggacaagt attgtctgt ttctgtcga cgaagacag 
      tattattcg atatgtgaa tcaagtttt gcacaatcc 
      tgatctggc tttacgtat cgccactag aaataatct 
      cgcaggcgc tgaagagct gtttgtgaa caagttcca 
      aatgttgtt cacgaatgg acagtatgc tgaagcggc 
      caaagtggc agccaatgc gccgaaagg gattttgag 
      aacaccagc tacgattca aatgttcca acaagtgcc 
      cacgcagcc gggacagaa cagtccact gttgcagta 
      tttcgggat tttgttaga tcaagggca gctaaacag 
      atacgaatc gctggaatt gtgcaaacc agtcctctt 
      gcaaggccg taaacaact tctcgaaaa atggctaaa 
      agaagacaa actcgaatg ttcggaaga attgggcga 
      tctcgtaaa acaagccga ctcaactct cgccctttc 
      tgtctatct ccgagctaa cgtccccgc caaagtcat 
      tcaaagttt tgccgaaac cggtcagtt ccaaaaaat 
      cgtcctcta cgccaaaaa agtcaacta cacacccga 
      ctacattta cttgctgcg ctccgtcat gcggacaaa 
      ccccgacca gggggcggc tttcgcaag catgctggt 
      tgccgacga ggaaccact agccgacat caaccaaat 
      tgtcgatat tttcatgga acaaaacat ggtgcaaca 
      gtgcacggc cttcttgct ggacgcttt gaagcacaa 
      taggccgac cgaaggcca cctccagac gcgcctcct 
      ggagatgaa cttaatgtc ggcgccgca ggtcgccga 
      cgcaatcct gggcaacaa catgtttac gcattatga 
      cagagccca tattgcgca gctttgcga gaaagccgg 
      gcttttgca gagggcgct ggagcacta caccgactt 
      gtacgacat caagcgggc tgtggtcca cacccactt 
      gctgccaat ggattggct ggtgaattt cttcgggac 
      tttgagcgt tgaggacag tttggagtg tctcaaggc 
      tatgttgac ggccaatat aaggcaaaa cttgcagat 
      ttgtgtaca aattgcgac caaatatca cgaacaact 
      tacaacgaa agcgctgat tgatttgtt tgagagttt 
      taagagtta tgaagggct tttctattt tctgggttc 
      gattgtgaa tttttcaca ggatccgga tgtgcattt 
      caagtatat tcaggcggc gtgtaagac cggacaaat 
      caaggaagt tgaacggat ttgtcgaga gtcgaattg 
      ttataatcc ggagagtgt gaagaattt cctcaagga 
      agcgaaact gaccgatca attgccgtt gattattgt 
      gtgcgatcg cttcgattt cgtgcacga tttggtgct 
      gtatttgta caggaattc gctgcaaaa gtacatcga 
      gatctacgt acaaaaggt aaatcccag ccgtctccc 
      cgtggtcgt cggcggcct cctggacgt agactgcgc 
      cgaagacat catcaaaaa cctgatcct cgtcgtccg 
      tggccaatt ctccaccga cgaactggt cgaagaagt 
      ggagaaacg caaccgctt gaaactcct cctaccatg 
      gctggagag tcgcgtcca cgagggctg tgtcgaacc 
      ggccaccca caacgccct ggccaaaat ctacatcga 
      ttcaaacaa caacgccga acgtttcct caaagaaaa 
      ccaatggta cgactcacg cgtcgttgg ccgctactg 
      cgaaaaacg cgaccccca tctcgcctg cgtcgccta 
      cgagcgcgg ccaatgcga ccgcgaact aatcgcagt 
      ctgtaacga aaactcgct tttcaaatc cgaggcgag 
      atatttagt cagacggcg ggacccgga attatgggc 
      cgaagtctt gcaggaaag taacccgta caggagaca 
      attgatcga tcaagtggt ccagacagc gctgagcga 
      aacgcaaga cccggagga catctccgt gactgtcaa 
      agccttcat gactgctga ccttccaaa tgaattgat 
      cgagttgct tgagaaaat cgtcctgga cagttcggt 
      tttctccga tcaccgaaa cttgcaaaa cctcctgat 
      cctaacggc gatcaaagc cgatgccac ccgagtcat 
      ggactacat caaccgtct agataatta cgacgctcc 
      tgatatcgc aaatatcgc aataaataa ccatttata 
      cgaggaggc gtttgcaat tttcaagaa attcgacgt 
      taacacttc agccattca ggttctgat cgagcaggt 
      gaacaattt ggatcgtgc gtatgagtt tgcggagcg 
      ttgtaatga gccggcggt ttggagtca gttggccaa 
      ggcacagtt gaatcaagg gttggtcaa ggaggcgat 
      cgattcgta cattaaggc cgatgatcc gtcggccta 
      tatggccgt ggtggagac ggcgtccaa gaataactc 
      gtgggagga tttggtgcg gtatttgca aatggcgag 
      gaagaagtc acgggagag ttatatcga gtcggagtt 
      gatttattc gtatgccaa gacgggacg gcttgccga 
      tttggagga gtttataag tgggccgaa tcatgccga 
      tatacagaa gatcggcga tcgatgttt tgatgataa 
      aatgtacga tgcggccaa gttgttgta taataatgt 
      ctcgaattt tgcgaggtt ggcgattac tttggtgca 
      tttgaagga gtttcaggg agctgtcga cagcgcccg 
      caaggccaa cagcacccg cacctggaa agaggtatg 
      ttttgcgtg cgtggacgc cgaagagtt ccgtttggc 
      gcaaatgtg cggaatgca cattgtcgt ccacgccga 
      cgaactgca agatctcat caactacta ccaagaccg 
      cggctattt cgaagagct catcggtct gctggaggc 
      cgccctagg tctcgagcg cgcccacat gggcatgtt 
      caccgagct ggccatcct ctattcgaa atacaagcc 
      ggcgaaaat gcgcgaaca cctggagct gttctggtc 
      gcgcgtcaa cattcccaa agtattgcg cgccgccga 
      acaggcgca tctctgggc cgaactggt ctttctcta 
      cgataagta cgaggagta cgataatgc cgttttggc 
      catgatggc gcatccgac ggaggcatg gcgtgaagg 
      acactttaa agatattat cactaaagt ggccaatat 
      tgagttgta ctacaaggc gattcagtt ttatttgga 
      ttataagcc gttgttgtt gaacgattt gttgctggt 
      tttggcacc gagaatgga tcatacgag ggctgtggc 
      gtttttcac aaagacggg acatttaca acttgtcaa 
      gtcttattt gaggtcggt gcagaattt gaataataa 
      ggccattaa tgaggcgct aaattcgct attgattga 
      agaagagga tttccaggg tttgagaac atcgatcga 
      cgcattcga caatttcga caatatcgg cttggccca 
      aaaactcga aaaacacga actgaccga attcagacg 
      catcgccgc ctatttata caaaggcaa caacagatg 
      gaaacagag cgtggaatt atgtaagaa ggatcgcct 
      tttcagaga tgcgatgga atacacagc tgaatccaa 
      aaatcaaga attggccga agaactact ggcgtggtt 
      tttggaacg aaaggccta tgattgttt tgcagcatg 
      tctctacca atgctatga tttattaag gcctgacgt 
      aatattgga actagcctg gagacataa aatcatgga 
      tttggcgat gccttattt gattcaagt aacgagaga 
      attaaccac aaaagtgga aaaattgga acaatcgga 
      tgcacaaag gcagagcga ggccgccga agaaacaca 
      caagccgat gatgatcaa cgagccaca gctaatgct 
      aacggcagg acctggcat gggtattcc accgcaggc 
      gtacgtccc cccgcaggc ctacgccca gccaggcta 
      cgcccccca gatgccgta cggcgctta cccgggcat 
      gtaatataa ggagaattt tagccagcg ttatgataa 
      ttacaagtt tagaagagg aaatggttc aaatgacgc 
      tactattaa taaaaatct ttttttatt ttaatcaca 
      aagtttact aaataaaaa attaatgtt atgtaatat 
      aatacaaag agagcagta actttggtt caaacaaca 
      gccattaac tttacgaat tagaacaat tgtttttcg 
      tttgatgtc gcgctataa atagtcact gtaaagctg 
      tacagtgta gcagaattt gttgataat tttaaaccc 
      attacacga aactgttga ttgtggaaa ttcgccggt 
      cgtagaggc tttaaggta attagctag ttcgtacaa 
      gttgatttt gttggtcat tatcgtact atttttttt 
      atttaactt ccattgtaa atgttagtg ttttatgag 
      taattttgt tatattatt tgcaaggtg ggtgccttt 
      tgttacaat gtacataat tgtaatatg taccgggca 
      aaagtacac tgtatatac cctttcgga agttgctac 
      tagacctca tccaacacc caccgcacg tatttctac 
      tctgattta tcaatgttt atcattccc atttatagt 
      gttatttaa aatcaaata tatgtatta agtatgaga 
      tcgtttaac ttatatatt gtcgcacct ttgactagg 
      ttttagcag ttattttat tatgattat cagctgaaa 
      taaacaagt acttgccca
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