Status
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Gene information
General information
TC033421
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015009,TC015010
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid42: 457923..467382
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033421
    iBeetle screen iB_02376
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033421 RA
      MYRKKVSDD EKNFQNQLH LEKELNALT RQYLVLDRD 
      CTHSQFSKG SSLIKGDKV IAIFQKEHV NVLTDLMRA 
      AAPGREKED EKLFDELSK LMYENRFYA DQVDKHKIY 
      LREIEGEIK IFQKSVHQL REQQTSDKQ CEERVWHAM 
      KTVEALENK LETQMKQFG TLCSKNKEL RYEIDHLLW 
      QRRIFLNQW QGLIDKLSD GKKIMLDLI EQATIAYNQ 
      REEWCNKLQ ILRTKAHHD LRNNIQEMR EIRQKYDQS 
      MKLRDFFET KGQRRVMKD LAAKEREKR RRMYEILEE 
      KVTKYHNML TNIQNFCRE KNLKNIAEK FAKQEEFNL 
      SKFKFVIER IQEMESVND RLGFLYLEI DEGFALHSE 
      RDEQQKNKL LELETSLEE MQTAAQESQ EKLDETDSF 
      FQSLLQGIS GLFQLCKCV KDPLFRLLG DNTTVQFYN 
      VQLYTQILE TRVQEMLII AGYRDKNRK PGDRKKIID 
      DRNYNNKIY DIDQIVQTT PCSLCVEHE MVSDVMDVL 
      QHVLSRDEA KAKLCERLQ LPGGTVRLH TISHSMPRQ 
      PLAPLTEEE KFKLHEQQE QELGCLQRQ YTLLEKDRD 
      QIACGAKLM RFDRVLSIY QKEHANIET DLEVASAPA 
      KKNQDLKDS NNLSQLIVD HEFYADEVK KERKYIQEI 
      EGQIKNVEK QVTQLRSQQ ITDKQHEER VWAAITTID 
      QLMNKLDVQ NKKFGVLCA KNRDMKLEI DRMLWERDV 
      FMGIWNKMI DKLVMGKKF MMDLIEQAT IAYDQREEW 
      CSKLQALRL KAHHDFILH VQEMRELKR KQDNDKKLE 
      EFFYVKGQK RIMRDLMRK EQFKRMQNK IKLKDRLNE 
      YKKYMNEIL EFANEQQVQ EIARKYYAQ EAENFSLFK 
      RLNNLIQEL EFLGDELDT AQTSIDQER ELHNARAQQ 
      QKNNLQSLE NTLQLATQE VEAAEQELK GVEGYINQM 
      LTGIETLFK ICRCNNDPV LQLLGNNAT INVYNAFFY 
      LEQVEKAVH EALIAVYSN ETKKKLPGA QRTVKDNRT 
      KGELRNVEQ FGVSNPCPL CIEYEMVGD VIDSLQHVL 
      TRDQAKAAL AERLKSPEM AEKLHNVSA CYLPKSREI 
      IQKRYQ
    CDS sequence
    • >TC033421 RA
      atgtatcga aaaaaagtt tctgatgac gaaaaaaac 
      ttccaaaac caactacac ctcgaaaaa gaattaaac 
      gccttgaca cgccagtat ctagttcta gaccgggat 
      tgtacccat agtcaattt tctaaaggc agttcttta 
      atcaaaggc gacaaggtg attgcaatt ttccaaaag 
      gagcatgtg aacgttctg actgactta atgagggct 
      gcagcccct ggtcgcgag aaagaagac gaaaaactc 
      ttcgacgaa ttatccaaa ctcatgtac gaaaaccgc 
      ttttacgcc gaccaagtc gataaacac aaaatctat 
      ttacgagaa attgaaggc gagataaaa atcttccaa 
      aaatcggtt caccaactt cgcgaacag caaaccagc 
      gacaaacag tgcgaagaa cgcgtctgg catgccatg 
      aaaacagtc gaggctttg gaaaacaaa ctagaaacg 
      caaatgaaa caattcggc acactttgc agcaaaaac 
      aaggaattg agatacgaa atcgaccac ttactgtgg 
      caaagacgc atttttcta aaccaatgg caaggcctc 
      attgacaaa ttatcagac ggtaaaaaa ataatgctg 
      gatttgatc gaacaagca acgattgcc tacaaccag 
      agggaagag tggtgcaac aagttgcag attttgcgc 
      accaaggca caccacgat ttgcgcaac aacatccaa 
      gaaatgcgc gaaatcagg caaaaatac gaccaaagt 
      atgaaactt cgcgacttt ttcgagacc aaaggccag 
      cgtcgagtc atgaaagac ctggcggcc aaagaacgg 
      gaaaagcgg cggcgcatg tacgaaatc ctcgaggaa 
      aaagtcacc aaataccac aacatgttg accaacatc 
      cagaatttt tgccgcgag aaaaacctc aaaaacatt 
      gcggaaaaa ttcgcaaaa caggaagag ttcaattta 
      tccaaattc aagttcgtt attgaaagg atccaagaa 
      atggagtcg gtgaatgat agactgggc ttcctgtat 
      ctggaaatc gacgaaggt tttgccctt cattccgaa 
      cgtgacgaa caacaaaaa aataagtta ttggaactt 
      gaaaccagc ttggaggaa atgcaaact gcggctcaa 
      gaatcccaa gagaagttg gacgagact gacagtttt 
      ttccaatca cttttgcaa ggaatttcg ggtcttttt 
      cagttgtgc aagtgtgtg aaggacccg ctgtttcgc 
      ctcctgggg gacaatacc accgtccag ttctacaac 
      gttcagctc tacacccag attttggaa acacgggtg 
      caggagatg ctgatcatt gctggttat agggacaag 
      aacaggaag ccgggcgat aggaaaaaa atcatcgat 
      gatcgaaat tacaataat aaaatttat gatatcgat 
      cagattgtt caaactacg ccgtgttct ttatgtgtt 
      gaacatgag atggtgtcg gatgttatg gacgtgtta 
      caacatgtg ttgtcacgt gatgaagct aaggcgaaa 
      ctttgtgaa agactgcaa ttgcctggg gggacagtg 
      aggcttcat acgatttcg cattccatg cctcgccaa 
      ccactggct cctctcacc gaggaggaa aaattcaaa 
      ttacacgaa caacaagaa caagaactt ggttgttta 
      cagcgccaa tacaccctt ctggaaaaa gaccgcgac 
      caaatcgcc tgtggcgcc aaactgatg cgctttgat 
      cgagttctc tcaatttac caaaaagag cacgcaaac 
      attgaaacc gatctagaa gtagcaagt gctcctgcc 
      aagaaaaac caagacctc aaagactcc aacaaccta 
      tcacagctg atcgtagac cacgaattt tacgcagac 
      gaagtcaaa aaagaacgg aaatacata caagaaatt 
      gagggtcaa atcaaaaac gttgaaaag caagtgacg 
      caactgcga tcgcaacaa atcacagac aaacaacac 
      gaggagcga gtctgggcc gcaattacc acaatcgac 
      caactgatg aacaaactg gacgttcag aacaaaaaa 
      ttcggggta ctttgtgcc aaaaaccgc gacatgaag 
      ctggaaatt gaccgaatg ttgtgggaa cgagacgtt 
      ttcatgggc atttggaac aagatgatt gataagttg 
      gtgatgggg aaaaaattt atgatggat ttgattgaa 
      caagcgacc attgcttac gatcagagg gaagagtgg 
      tgttccaag ttgcaagcc ttgaggctt aaagcacac 
      cacgatttt attcttcac gtccaggaa atgcgagaa 
      ttgaaacga aaacaagac aatgataaa aaattggag 
      gagtttttt tatgtcaaa ggacaaaag cggatcatg 
      agggatctg atgaggaag gaacagttc aagaggatg 
      cagaacaaa atcaagctc aaagaccgg ctgaacgaa 
      tacaaaaaa tacatgaac gagattttg gagtttgcc 
      aacgagcag caagttcaa gaaatcgca cgaaaatac 
      tacgcacaa gaggccgaa aatttcagt ttgttcaaa 
      cgcctcaac aatctcatc caagaactg gagtttctt 
      ggtgacgaa ctagacaca gctcagaca agcattgac 
      caagagaga gaactgcat aatgcgaga gcccaacaa 
      caaaaaaat aatttgcaa agtttggaa aacacctta 
      caattggcc actcaagaa gtagaagca gccgagcaa 
      gaactcaaa ggtgttgaa ggctatatt aaccaaatg 
      ttaaccggg attgagacg cttttcaaa atctgccgc 
      tgtaataac gatcctgtt ttgcaactt ctgggcaac 
      aatgcaacc atcaacgtt tacaacgcg tttttctac 
      ctggaacag gtcgaaaaa gccgttcat gaagccctg 
      attgcggtt tattccaac gaaactaaa aagaaactt 
      ccaggagcg cagagaacc gtcaaggat aacaggact 
      aaaggagag ctgcgaaat gtcgaacag tttggagtg 
      tcaaatcct tgtcctttg tgcattgaa tacgaaatg 
      gtgggtgat gtaattgac tccttacag cacgttttg 
      acgagagat caagccaaa gcagctcta gccgagcgt 
      ttgaaatcg ccggaaatg gccgaaaaa ttgcacaat 
      gtttccgct tgttattta cccaaatcg cgagaaatt 
      atccagaaa cggtaccag tga
    mRNA sequence
    • >TC033421 RA
      tgtgcttaa aatgtatcg aaaaaaagt ttctgatga 
      cgaaaaaaa cttccaaaa ccaactaca cctcgaaaa 
      agaattaaa cgccttgac acgccagta tctagttct 
      agaccggga ttgtaccca tagtcaatt ttctaaagg 
      cagttcttt aatcaaagg cgacaaggt gattgcaat 
      tttccaaaa ggagcatgt gaacgttct gactgactt 
      aatgagggc tgcagcccc tggtcgcga gaaagaaga 
      cgaaaaact cttcgacga attatccaa actcatgta 
      cgaaaaccg cttttacgc cgaccaagt cgataaaca 
      caaaatcta tttacgaga aattgaagg cgagataaa 
      aatcttcca aaaatcggt tcaccaact tcgcgaaca 
      gcaaaccag cgacaaaca gtgcgaaga acgcgtctg 
      gcatgccat gaaaacagt cgaggcttt ggaaaacaa 
      actagaaac gcaaatgaa acaattcgg cacactttg 
      cagcaaaaa caaggaatt gagatacga aatcgacca 
      cttactgtg gcaaagacg catttttct aaaccaatg 
      gcaaggcct cattgacaa attatcaga cggtaaaaa 
      aataatgct ggatttgat cgaacaagc aacgattgc 
      ctacaacca gagggaaga gtggtgcaa caagttgca 
      gattttgcg caccaaggc acaccacga tttgcgcaa 
      caacatcca agaaatgcg cgaaatcag gcaaaaata 
      cgaccaaag tatgaaact tcgcgactt tttcgagac 
      caaaggcca gcgtcgagt catgaaaga cctggcggc 
      caaagaacg ggaaaagcg gcggcgcat gtacgaaat 
      cctcgagga aaaagtcac caaatacca caacatgtt 
      gaccaacat ccagaattt ttgccgcga gaaaaacct 
      caaaaacat tgcggaaaa attcgcaaa acaggaaga 
      gttcaattt atccaaatt caagttcgt tattgaaag 
      gatccaaga aatggagtc ggtgaatga tagactggg 
      cttcctgta tctggaaat cgacgaagg ttttgccct 
      tcattccga acgtgacga acaacaaaa aaataagtt 
      attggaact tgaaaccag cttggagga aatgcaaac 
      tgcggctca agaatccca agagaagtt ggacgagac 
      tgacagttt tttccaatc acttttgca aggaatttc 
      gggtctttt tcagttgtg caagtgtgt gaaggaccc 
      gctgtttcg cctcctggg ggacaatac caccgtcca 
      gttctacaa cgttcagct ctacaccca gattttgga 
      aacacgggt gcaggagat gctgatcat tgctggtta 
      tagggacaa gaacaggaa gccgggcga taggaaaaa 
      aatcatcga tgatcgaaa ttacaataa taaaattta 
      tgatatcga tcagattgt tcaaactac gccgtgttc 
      tttatgtgt tgaacatga gatggtgtc ggatgttat 
      ggacgtgtt acaacatgt gttgtcacg tgatgaagc 
      taaggcgaa actttgtga aagactgca attgcctgg 
      ggggacagt gaggcttca tacgatttc gcattccat 
      gcctcgcca accactggc tcctctcac cgaggagga 
      aaaattcaa attacacga acaacaaga acaagaact 
      tggttgttt acagcgcca atacaccct tctggaaaa 
      agaccgcga ccaaatcgc ctgtggcgc caaactgat 
      gcgctttga tcgagttct ctcaattta ccaaaaaga 
      gcacgcaaa cattgaaac cgatctaga agtagcaag 
      tgctcctgc caagaaaaa ccaagacct caaagactc 
      caacaacct atcacagct gatcgtaga ccacgaatt 
      ttacgcaga cgaagtcaa aaaagaacg gaaatacat 
      acaagaaat tgagggtca aatcaaaaa cgttgaaaa 
      gcaagtgac gcaactgcg atcgcaaca aatcacaga 
      caaacaaca cgaggagcg agtctgggc cgcaattac 
      cacaatcga ccaactgat gaacaaact ggacgttca 
      gaacaaaaa attcggggt actttgtgc caaaaaccg 
      cgacatgaa gctggaaat tgaccgaat gttgtggga 
      acgagacgt tttcatggg catttggaa caagatgat 
      tgataagtt ggtgatggg gaaaaaatt tatgatgga 
      tttgattga acaagcgac cattgctta cgatcagag 
      ggaagagtg gtgttccaa gttgcaagc cttgaggct 
      taaagcaca ccacgattt tattcttca cgtccagga 
      aatgcgaga attgaaacg aaaacaaga caatgataa 
      aaaattgga ggagttttt ttatgtcaa aggacaaaa 
      gcggatcat gagggatct gatgaggaa ggaacagtt 
      caagaggat gcagaacaa aatcaagct caaagaccg 
      gctgaacga atacaaaaa atacatgaa cgagatttt 
      ggagtttgc caacgagca gcaagttca agaaatcgc 
      acgaaaata ctacgcaca agaggccga aaatttcag 
      tttgttcaa acgcctcaa caatctcat ccaagaact 
      ggagtttct tggtgacga actagacac agctcagac 
      aagcattga ccaagagag agaactgca taatgcgag 
      agcccaaca acaaaaaaa taatttgca aagtttgga 
      aaacacctt acaattggc cactcaaga agtagaagc 
      agccgagca agaactcaa aggtgttga aggctatat 
      taaccaaat gttaaccgg gattgagac gcttttcaa 
      aatctgccg ctgtaataa cgatcctgt tttgcaact 
      tctgggcaa caatgcaac catcaacgt ttacaacgc 
      gtttttcta cctggaaca ggtcgaaaa agccgttca 
      tgaagccct gattgcggt ttattccaa cgaaactaa 
      aaagaaact tccaggagc gcagagaac cgtcaagga 
      taacaggac taaaggaga gctgcgaaa tgtcgaaca 
      gtttggagt gtcaaatcc ttgtccttt gtgcattga 
      atacgaaat ggtgggtga tgtaattga ctccttaca 
      gcacgtttt gacgagaga tcaagccaa agcagctct 
      agccgagcg tttgaaatc gccggaaat ggccgaaaa 
      attgcacaa tgtttccgc ttgttattt acccaaatc 
      gcgagaaat tatccagaa acggtacca gtgactgcg 
      agctggctt tagttaata aattttgat caaagtt