Status
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Gene information
General information
TC033419
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014999,TC030178
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4894287..4905991
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033419
    iBeetle screen iB_02375

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    gena triplet bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs potentially swollen - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033419 RA
      MEASQESIS SRRDKLNTQ IIGDQKLTL INRECLLDA 
      LQALYDECS LDALQKYDK QIAQFVSKY KNTLLEVKK 
      LRVNETDFE IKDVIGRGH FGEVHVVKE KQTGDIYAM 
      KTIRKCDSF EKTNFDVER DIMAFAASP WITSLQYAF 
      QDSVYLYYV MEYHPGGDL LGLLYRQGG TLPESAATF 
      YLAEIVLAL EDLHSMGFV HRDIKPDNI LLDRCGHLK 
      LVDFGSAAK LNQQGAVDK GLPVGTPDY IAPEVLQSL 
      ERNSKSHYG VSCDFWSLG ALAFELVVG KPPFSGQNT 
      SVTYSRIIN HSNNLKFPQ DVCLTQAYV SLVRGLLTE 
      AKARLRGPQ IKKHPLFKH IHFDSLRDQ VPPYVPKIS 
      SMEDTSNFA DVGAKKTTP SMENFKKKT QFSGRNLPF 
      IGFTFTPGA SSCGREFDR NSRAKDEII RNMREELEV 
      VRRDLMKRE DFEEVKLVL EKKILEKNQ KLENIESVR 
      EKLERDLAA SMAESTALK RTLEIERKD RAELERKAL 
      DLIKSAKIK WETSERNKI ESLKLEIEQ QKEKIAQLG 
      TTNKMLNEQ LQKAFKMEN KHKESIEAV EHLNRRSVI 
      GLESRIEKV ALDSKQKIM SLQATLDET IKQKKELEF 
      EIEKLKDSE DELRKKLSK SCDDYTVLN GAALEAEKL 
      VKELGQKVG DFEREFEKA ESYKAKIKT LSREIDDFK 
      AKIRDLENA NLLLKEENK SIEEFKNQI NSLKDTKVQ 
      NERKINELE SKLQEEEQK CVTLKKELD NSQNLINES 
      QQLKELRVK MWKMEKELG NAKIDKRIV ERELKDAEN 
      EIKNLNEEI KNLKDKIEE NRKIHSAAL LELNNINEN 
      LSLEMVKVR ESHKNLEEN LESEKNRNG EEKQLIVQL 
      RDTISEKNK TISTIQSQL QSSRHELAT LEEKNQKLL 
      ESNLKKSKQ FEEISNEKE KLSREVNET KRQLENAQT 
      NLEALREAC LIIENQVIE YEKINSSIQ AEKSSLAEN 
      TEKLIKDLC KAKEEIQEA KKATNEEKS LRLLAETKA 
      KRLAEDVEL LQNECEAYK DQCFKFKEC SNNLSEELT 
      LSEERVSDL EVTLKSYER QIEDFVSES NILKEEISQ 
      YLTQMAALK ESNFKLKQQ IQDLKEARN VLVEKVEEL 
      ERVLNGKVN YYEEREMKN ESIIKQQTK LIEYLQVKI 
      DEHNSKKRT LTDKLFGSK KENVQPLVL PVNKDLEGQ 
      LAKQKQINK NLQEEIYKL KAELNCRTP ITKVQRHKS 
      EIVSPKTRS PQIISHKFI LKLCKNLTR CDHCAKQIM 
      VGRDVNTCT KCHVNVHQN CVSSVKSTC GLPNTCVKN 
      RKSESNVVI YEGWIKLLT KNNTWDKKY ACLTDSKLN 
      VYLTKDKKS LTESFDLKL PNSRKKVVL EPLPSEIGV 
      NVASTDLPF VMKVEVLVQ GGPMVSMVF MALSVEERN 
      QWYKAFQEL FDEAAGQHL EAIFTLPDQ IYANCVLDL 
      TKNIKVLGT DQGLFSYHN DQLVHIEGP TDIHHISII 
      PNSNTVVMI SDLTSILIS CDLNHLINL TQCAPCTKP 
      VLNYRTINI KEMDGFHYL QTSKFPKHQ LFCAATCRQ 
      LVIVKYDCE NYEFVPLRI MDTAEPLGC ALFTEHSLI 
      VGADKFFEI DLATFKAEE FLDGSDARL KFAVKCHKM 
      QSFPVAILE ISQNPKEFL VCFNEFSVF VDEYGRSSR 
      EEELKTSYL PIGFHFVAP FLYIIQYSG VEVLKVGGN 
      VANVTVDLK NMRFLGESP NGIYLYHDG RVKLLEGRR 
      VVDCDDVSS VSQSEAGDE DRFSFTSSM VQSLDGNSS 
      DLENSSEEN LFLEHYEVI NRQTLDTAD VKTLTTVVE 
      KECYKMKVT IDAFNDIFG WSFLLFTLY SSLLLLDYV 
      SCVVRNEYK YKSDKMAEI ISSDLLQLC IFFVALIIL 
      ILQCGRVRS EVVKILNVL KKIALRRQT TNGDLDELI 
      VTLSTHYFP VFSACDYFT IETKTVLDV LGVTVTYLI 
      VIIQFDDKI VQV
    CDS sequence
    • >TC033419 RA
      atggaagcg tcccaagag tccatcagt tcccgaagg 
      gacaaactc aacactcaa ataatcggc gaccaaaaa 
      ctcaccctc atcaaccga gaatgcctc ctggacgcc 
      cttcaggcc ctttacgac gaatgcagt ctggacgct 
      ttgcaaaaa tacgacaaa caaatcgcc caatttgtg 
      agcaagtac aaaaacacg ctccttgag gtgaaaaaa 
      ctgcgtgtg aatgagacc gatttcgag ataaaggat 
      gtgatagga cgggggcac tttggggag gtgcatgtc 
      gtgaaggag aagcaaact ggggatatt tacgccatg 
      aagacgata cgaaagtgt gacagtttc gagaaaact 
      aactttgat gttgagcga gatataatg gcctttgcg 
      gcttcgccc tggatcaca agtctccag tacgcgttc 
      caggacagt gtctacttg tactacgtg atggagtac 
      caccctggg ggcgacttg ctggggctc ttgtaccgc 
      caagggggc accttgccc gaaagcgcc gccaccttc 
      tacctcgcc gaaattgtc ctcgctctg gaagacctg 
      cactcgatg gggttcgtc caccgggac atcaaaccc 
      gacaacatc ctcctcgac cgctgcggc cacctaaag 
      ttggtcgac ttcggctca gccgccaaa ctcaaccag 
      cagggggcc gtggacaag gggcttccg gtgggcacc 
      cccgactac atcgccccc gaggtgctc cagtccctc 
      gagcgcaac tccaagtcg cactacggc gtgtcgtgc 
      gatttctgg tcgttgggg gccctcgcc ttcgagctg 
      gtcgtgggc aagcccccg ttttcgggg caaaacacc 
      tcagtcacc tactcgcgg ataatcaac cactcgaac 
      aatttgaaa ttcccccag gatgtgtgc ttgacacaa 
      gcctacgtc tccctggtt cggggtctc ctgactgag 
      gcgaaggcc cgcctgagg gggccccag atcaagaaa 
      caccccctt ttcaagcac attcatttc gattcgttg 
      cgcgatcaa gtgcccccc tatgtcccc aaaatcagc 
      tcgatggag gatacgagt aattttgca gacgtgggg 
      gcgaagaaa accacccca agtatggag aacttcaag 
      aagaagacg cagttttcc gggaggaat ttgcccttt 
      attgggttt acgttcacc ccaggggcg agctcttgt 
      gggagggag tttgatcgg aattcgagg gccaaggac 
      gagattatt aggaatatg agggaagag ttggaggtg 
      gtgaggagg gatttgatg aaacgggag gattttgag 
      gaggtcaag ttggttttg gagaaaaag attctcgag 
      aaaaaccag aagttggag aatattgaa agtgtgagg 
      gagaagttg gagagggat ttggctgca agcatggcc 
      gagagtacg gcattgaaa agaactctc gaaatcgag 
      cgaaaagac cgcgccgaa ctcgaacgc aaagccctc 
      gacttaata aaatcggca aaaatcaaa tgggaaaca 
      tccgaacgc aacaaaatc gaatcatta aaactagaa 
      attgagcaa caaaaagaa aaaatcgcc caacttgga 
      accaccaac aaaatgcta aacgaacaa ttacaaaaa 
      gctttcaaa atggaaaac aaacataaa gaatcgata 
      gaagcagtc gaacactta aatcggcga agcgtaata 
      ggacttgag tcacgaatt gaaaaagtc gccttagat 
      tcgaaacaa aagattatg agtcttcaa gccacctta 
      gacgaaaca attaaacaa aagaaggag ttagagttt 
      gaaattgag aaattaaaa gacagtgag gacgaattg 
      aggaaaaaa ctgtcgaaa tcttgtgat gattatact 
      gttctgaat ggagcggct ctcgaggcc gaaaaactc 
      gttaaagaa cttgggcag aaagtgggc gattttgag 
      cgtgaattt gaaaaggcg gaaagctac aaagcgaag 
      atcaagact ttaagtcgg gaaattgac gattttaag 
      gcgaaaatt cgtgatttg gaaaatgcg aatttgtta 
      ctcaaagag gaaaataag agtattgaa gagtttaag 
      aatcagata aattcacta aaagacact aaggtacaa 
      aatgagcga aaaatcaac gaactggag agcaagtta 
      caagaagag gagcaaaaa tgtgtcaca ctgaagaaa 
      gaattggac aattcgcaa aatctaatc aacgagagt 
      caacagttg aaggaattg agggtaaaa atgtggaaa 
      atggagaag gagttaggt aatgcaaag atcgacaaa 
      cgaatagtt gagcgtgaa cttaaagat gcagaaaat 
      gaaataaaa aatctcaat gaagaaata aaaaatttg 
      aaagataag atagaagag aatcggaaa attcactcc 
      gccgccttg ttagaattg aacaatatt aacgaaaac 
      ctgtctttg gaaatggtc aaagtcagg gaatcccac 
      aaaaacttg gaagaaaac ctcgaaagt gagaagaac 
      cgaaacggt gaagagaaa caactaatc gtccaactc 
      agagacact atcagcgaa aagaacaaa acaatttcc 
      acaatccag tcccaactg caatcttcc agacacgaa 
      ctcgcaacc cttgaagag aaaaaccag aaattgctc 
      gaatcaaac ctgaaaaag tccaaacaa tttgaagaa 
      atctcgaac gaaaaagag aaattatca agggaagtg 
      aacgaaacg aagagacaa ttagaaaac gcccaaacg 
      aatctagaa gccttgcgt gaggcttgc ctcatcatc 
      gaaaatcaa gtgatcgag tacgaaaaa atcaactct 
      tcgatccag gccgaaaaa tcgagtctt gccgaaaac 
      accgagaaa ctgatcaag gatttgtgc aaagccaag 
      gaggaaatc caggaggcg aagaaggcc accaatgag 
      gagaaatcg ctgcggcta ttggccgaa actaaggcg 
      aaacgattg gcggaggat gtggagttg ttgcagaat 
      gagtgtgag gcttacaaa gatcagtgt tttaaattt 
      aaagagtgt tcgaataat ttgagtgaa gagctgacg 
      ctttcggag gagagggtc tccgatttg gaggttacc 
      ctcaaatcg tacgagagg caaattgag gatttcgtc 
      tccgaaagt aacatcttg aaagaggag atttcgcaa 
      tatttgact caaatggcc gccctcaaa gagtcgaat 
      ttcaaactc aaacagcaa attcaggac ttgaaggag 
      gcgcggaat gtactagtg gagaaggtt gaggagttg 
      gagagggtc ctcaatggt aaagtcaac tattacgaa 
      gaacgggag atgaagaac gagtcgatt attaagcaa 
      caaaccaag ctgattgag tatctccaa gttaagatt 
      gacgaacac aacagtaaa aaacgaaca ctgactgat 
      aaattgttc ggttcgaag aaagaaaat gtgcaacct 
      ttggtcctt ccggtcaat aaagacctg gaaggacaa 
      ttggcgaaa caaaaacaa atcaacaaa aacctacaa 
      gaggaaata tacaaattg aaggcggag cttaactgt 
      cgcacacca atcacaaaa gtgcaacgt cacaaatcg 
      gaaatcgtg tcacctaag accagatcg cctcaaata 
      atatcacac aaatttata ctaaagctt tgtaaaaac 
      ttgacacgt tgtgaccac tgcgccaaa caaatcatg 
      gtgggccgt gacgtcaac acatgtacg aaatgtcac 
      gtcaacgtg catcaaaat tgtgtcagt tctgtcaaa 
      agtacttgc ggcttgcct aacacatgt gtcaaaaac 
      cgtaaaagc gaatcaaat gtcgtaatt tacgaaggt 
      tggattaaa ttacttact aaaaacaac acttgggac 
      aaaaaatac gcttgtttg actgattcc aaattgaat 
      gtttattta actaaagat aagaaaagt ctgacggaa 
      tcgtttgat ttgaaattg ccaaattcg aggaagaaa 
      gttgttttg gaaccgctg ccgtctgaa atcggggtg 
      aatgttgcc agtacggac ctgccgttt gtgatgaaa 
      gtggaggtt ttggtacag ggtgggccg atggtttcg 
      atggttttt atggccttg tcggtggag gagaggaat 
      cagtggtat aaagcattt caagagctc ttcgacgaa 
      gccgccggc caacacctg gaggccatc ttcaccctc 
      cctgaccaa atttacgcc aactgcgtc ctggatctc 
      accaaaaac atcaaagtt ttaggcacc gaccaaggt 
      ctattttcc taccacaac gatcaactt gtacatatc 
      gagggcccc accgacata catcacata tcaataatc 
      cccaactct aacaccgtg gtcatgatc tcagaccta 
      actagcatt ttaatctca tgtgactta aaccacctc 
      attaattta acccaatgc gcaccttgt actaaacca 
      gtccttaat tatcgcaca atcaatatc aaagaaatg 
      gacggtttt cactacttg caaacgtcg aaattcccc 
      aaacaccaa ttattttgt gccgcgact tgtcgccag 
      ttggtaatc gtgaaatac gactgcgaa aattacgaa 
      ttcgttccg ttgcggatt atggacacg gccgaaccg 
      ctggggtgt gcgttattt acggaacac tcgctgatt 
      gtgggcgct gataaattt ttcgaaatc gatttagcc 
      actttcaag gccgaggaa ttcctcgat gggtcagac 
      gcgaggttg aaattcgca gtcaagtgt cacaaaatg 
      cagtcgttt ccggtcgca attttggaa atttcgcaa 
      aatccgaag gagtttttg gtctgtttt aacgaattt 
      tcggtgttt gtggacgag tatgggcgg agttcgcgc 
      gaggaggag ttgaaaacg agttatctg ccaattggg 
      ttccacttt gtggcgcct tttttgtat attattcag 
      tacagtgga gtggaagtt ttgaaagtt ggtggaaac 
      gtggcgaat gtgacggtt gatttgaaa aacatgcgg 
      tttttgggg gaaagcccg aacgggatt tatctctat 
      catgacgga agagttaag ttgttggag gggcgcaga 
      gtggttgat tgtgacgat gtcagttcg gtcagccaa 
      tcggaggcc ggggatgag gataggttt agttttacg 
      agctcgatg gtgcaaagt ctcgatggg aattccagc 
      gatttggag aacagtagc gaggagaat ttgtttttg 
      gaacattac gaagtcata aatagacaa acgcttgac 
      actgcagat gtcaaaact ttgacaaca gtcgtcgag 
      aaagagtgt tacaaaatg aaagtcacc attgatgcg 
      tttaatgat attttcggc tggtctttc ctacttttt 
      acgctatat tccagcctt ttacttctc gactacgtc 
      agttgtgtg gtaaggaat gaatataag tacaaatcg 
      gacaaaatg gccgaaatc atatcatca gatttgcta 
      caactttgc atttttttc gtcgccctg attattttg 
      atattgcaa tgcgggaga gtacgatca gaagtcgta 
      aaaatctta aatgtgctt aagaaaatt gccttaagg 
      cgccaaaca accaatggc gacttggac gaacttatc 
      gtcacgctt tcaactcat tattttccg gttttcagc 
      gcttgtgat tatttcact attgaaact aagaccgtg 
      ttggatgtt ttgggggtc actgtgacc tacctgatc 
      gttattata caatttgac gataaaata gtgcaagtt 
      tag
    mRNA sequence
    • >TC033419 RA
      tgtaacggt tttttcaaa tttcaatca aacccgtgt 
      ttactttcc acttttccg tgtttaaaa tgtgtgtat 
      gagtgtgtt tttgtcatg gaagcgtcc caagagtcc 
      atcagttcc cgaagggac aaactcaac actcaaata 
      atcggcgac caaaaactc accctcatc aaccgagaa 
      tgcctcctg gacgccctt caggccctt tacgacgaa 
      tgcagtctg gacgctttg caaaaatac gacaaacaa 
      atcgcccaa tttgtgagc aagtacaaa aacacgctc 
      cttgaggtg aaaaaactg cgtgtgaat gagaccgat 
      ttcgagata aaggatgtg ataggacgg gggcacttt 
      ggggaggtg catgtcgtg aaggagaag caaactggg 
      gatatttac gccatgaag acgatacga aagtgtgac 
      agtttcgag aaaactaac tttgatgtt gagcgagat 
      ataatggcc tttgcggct tcgccctgg atcacaagt 
      ctccagtac gcgttccag gacagtgtc tacttgtac 
      tacgtgatg gagtaccac cctgggggc gacttgctg 
      gggctcttg taccgccaa gggggcacc ttgcccgaa 
      agcgccgcc accttctac ctcgccgaa attgtcctc 
      gctctggaa gacctgcac tcgatgggg ttcgtccac 
      cgggacatc aaacccgac aacatcctc ctcgaccgc 
      tgcggccac ctaaagttg gtcgacttc ggctcagcc 
      gccaaactc aaccagcag ggggccgtg gacaagggg 
      cttccggtg ggcaccccc gactacatc gcccccgag 
      gtgctccag tccctcgag cgcaactcc aagtcgcac 
      tacggcgtg tcgtgcgat ttctggtcg ttgggggcc 
      ctcgccttc gagctggtc gtgggcaag cccccgttt 
      tcggggcaa aacacctca gtcacctac tcgcggata 
      atcaaccac tcgaacaat ttgaaattc ccccaggat 
      gtgtgcttg acacaagcc tacgtctcc ctggttcgg 
      ggtctcctg actgaggcg aaggcccgc ctgaggggg 
      ccccagatc aagaaacac ccccttttc aagcacatt 
      catttcgat tcgttgcgc gatcaagtg cccccctat 
      gtccccaaa atcagctcg atggaggat acgagtaat 
      tttgcagac gtgggggcg aagaaaacc accccaagt 
      atggagaac ttcaagaag aagacgcag ttttccggg 
      aggaatttg ccctttatt gggtttacg ttcacccca 
      ggggcgagc tcttgtggg agggagttt gatcggaat 
      tcgagggcc aaggacgag attattagg aatatgagg 
      gaagagttg gaggtggtg aggagggat ttgatgaaa 
      cgggaggat tttgaggag gtcaagttg gttttggag 
      aaaaagatt ctcgagaaa aaccagaag ttggagaat 
      attgaaagt gtgagggag aagttggag agggatttg 
      gctgcaagc atggccgag agtacggca ttgaaaaga 
      actctcgaa atcgagcga aaagaccgc gccgaactc 
      gaacgcaaa gccctcgac ttaataaaa tcggcaaaa 
      atcaaatgg gaaacatcc gaacgcaac aaaatcgaa 
      tcattaaaa ctagaaatt gagcaacaa aaagaaaaa 
      atcgcccaa cttggaacc accaacaaa atgctaaac 
      gaacaatta caaaaagct ttcaaaatg gaaaacaaa 
      cataaagaa tcgatagaa gcagtcgaa cacttaaat 
      cggcgaagc gtaatagga cttgagtca cgaattgaa 
      aaagtcgcc ttagattcg aaacaaaag attatgagt 
      cttcaagcc accttagac gaaacaatt aaacaaaag 
      aaggagtta gagtttgaa attgagaaa ttaaaagac 
      agtgaggac gaattgagg aaaaaactg tcgaaatct 
      tgtgatgat tatactgtt ctgaatgga gcggctctc 
      gaggccgaa aaactcgtt aaagaactt gggcagaaa 
      gtgggcgat tttgagcgt gaatttgaa aaggcggaa 
      agctacaaa gcgaagatc aagacttta agtcgggaa 
      attgacgat tttaaggcg aaaattcgt gatttggaa 
      aatgcgaat ttgttactc aaagaggaa aataagagt 
      attgaagag tttaagaat cagataaat tcactaaaa 
      gacactaag gtacaaaat gagcgaaaa atcaacgaa 
      ctggagagc aagttacaa gaagaggag caaaaatgt 
      gtcacactg aagaaagaa ttggacaat tcgcaaaat 
      ctaatcaac gagagtcaa cagttgaag gaattgagg 
      gtaaaaatg tggaaaatg gagaaggag ttaggtaat 
      gcaaagatc gacaaacga atagttgag cgtgaactt 
      aaagatgca gaaaatgaa ataaaaaat ctcaatgaa 
      gaaataaaa aatttgaaa gataagata gaagagaat 
      cggaaaatt cactccgcc gccttgtta gaattgaac 
      aatattaac gaaaacctg tctttggaa atggtcaaa 
      gtcagggaa tcccacaaa aacttggaa gaaaacctc 
      gaaagtgag aagaaccga aacggtgaa gagaaacaa 
      ctaatcgtc caactcaga gacactatc agcgaaaag 
      aacaaaaca atttccaca atccagtcc caactgcaa 
      tcttccaga cacgaactc gcaaccctt gaagagaaa 
      aaccagaaa ttgctcgaa tcaaacctg aaaaagtcc 
      aaacaattt gaagaaatc tcgaacgaa aaagagaaa 
      ttatcaagg gaagtgaac gaaacgaag agacaatta 
      gaaaacgcc caaacgaat ctagaagcc ttgcgtgag 
      gcttgcctc atcatcgaa aatcaagtg atcgagtac 
      gaaaaaatc aactcttcg atccaggcc gaaaaatcg 
      agtcttgcc gaaaacacc gagaaactg atcaaggat 
      ttgtgcaaa gccaaggag gaaatccag gaggcgaag 
      aaggccacc aatgaggag aaatcgctg cggctattg 
      gccgaaact aaggcgaaa cgattggcg gaggatgtg 
      gagttgttg cagaatgag tgtgaggct tacaaagat 
      cagtgtttt aaatttaaa gagtgttcg aataatttg 
      agtgaagag ctgacgctt tcggaggag agggtctcc 
      gatttggag gttaccctc aaatcgtac gagaggcaa 
      attgaggat ttcgtctcc gaaagtaac atcttgaaa 
      gaggagatt tcgcaatat ttgactcaa atggccgcc 
      ctcaaagag tcgaatttc aaactcaaa cagcaaatt 
      caggacttg aaggaggcg cggaatgta ctagtggag 
      aaggttgag gagttggag agggtcctc aatggtaaa 
      gtcaactat tacgaagaa cgggagatg aagaacgag 
      tcgattatt aagcaacaa accaagctg attgagtat 
      ctccaagtt aagattgac gaacacaac agtaaaaaa 
      cgaacactg actgataaa ttgttcggt tcgaagaaa 
      gaaaatgtg caacctttg gtccttccg gtcaataaa 
      gacctggaa ggacaattg gcgaaacaa aaacaaatc 
      aacaaaaac ctacaagag gaaatatac aaattgaag 
      gcggagctt aactgtcgc acaccaatc acaaaagtg 
      caacgtcac aaatcggaa atcgtgtca cctaagacc 
      agatcgcct caaataata tcacacaaa tttatacta 
      aagctttgt aaaaacttg acacgttgt gaccactgc 
      gccaaacaa atcatggtg ggccgtgac gtcaacaca 
      tgtacgaaa tgtcacgtc aacgtgcat caaaattgt 
      gtcagttct gtcaaaagt acttgcggc ttgcctaac 
      acatgtgtc aaaaaccgt aaaagcgaa tcaaatgtc 
      gtaatttac gaaggttgg attaaatta cttactaaa 
      aacaacact tgggacaaa aaatacgct tgtttgact 
      gattccaaa ttgaatgtt tatttaact aaagataag 
      aaaagtctg acggaatcg tttgatttg aaattgcca 
      aattcgagg aagaaagtt gttttggaa ccgctgccg 
      tctgaaatc ggggtgaat gttgccagt acggacctg 
      ccgtttgtg atgaaagtg gaggttttg gtacagggt 
      gggccgatg gtttcgatg gtttttatg gccttgtcg 
      gtggaggag aggaatcag tggtataaa gcatttcaa 
      gagctcttc gacgaagcc gccggccaa cacctggag 
      gccatcttc accctccct gaccaaatt tacgccaac 
      tgcgtcctg gatctcacc aaaaacatc aaagtttta 
      ggcaccgac caaggtcta ttttcctac cacaacgat 
      caacttgta catatcgag ggccccacc gacatacat 
      cacatatca ataatcccc aactctaac accgtggtc 
      atgatctca gacctaact agcatttta atctcatgt 
      gacttaaac cacctcatt aatttaacc caatgcgca 
      ccttgtact aaaccagtc cttaattat cgcacaatc 
      aatatcaaa gaaatggac ggttttcac tacttgcaa 
      acgtcgaaa ttccccaaa caccaatta ttttgtgcc 
      gcgacttgt cgccagttg gtaatcgtg aaatacgac 
      tgcgaaaat tacgaattc gttccgttg cggattatg 
      gacacggcc gaaccgctg gggtgtgcg ttatttacg 
      gaacactcg ctgattgtg ggcgctgat aaatttttc 
      gaaatcgat ttagccact ttcaaggcc gaggaattc 
      ctcgatggg tcagacgcg aggttgaaa ttcgcagtc 
      aagtgtcac aaaatgcag tcgtttccg gtcgcaatt 
      ttggaaatt tcgcaaaat ccgaaggag tttttggtc 
      tgttttaac gaattttcg gtgtttgtg gacgagtat 
      gggcggagt tcgcgcgag gaggagttg aaaacgagt 
      tatctgcca attgggttc cactttgtg gcgcctttt 
      ttgtatatt attcagtac agtggagtg gaagttttg 
      aaagttggt ggaaacgtg gcgaatgtg acggttgat 
      ttgaaaaac atgcggttt ttgggggaa agcccgaac 
      gggatttat ctctatcat gacggaaga gttaagttg 
      ttggagggg cgcagagtg gttgattgt gacgatgtc 
      agttcggtc agccaatcg gaggccggg gatgaggat 
      aggtttagt tttacgagc tcgatggtg caaagtctc 
      gatgggaat tccagcgat ttggagaac agtagcgag 
      gagaatttg tttttggaa cattacgaa gtcataaat 
      agacaaacg cttgacact gcagatgtc aaaactttg 
      acaacagtc gtcgagaaa gagtgttac aaaatgaaa 
      gtcaccatt gatgcgttt aatgatatt ttcggctgg 
      tctttccta ctttttacg ctatattcc agcctttta 
      cttctcgac tacgtcagt tgtgtggta aggaatgaa 
      tataagtac aaatcggac aaaatggcc gaaatcata 
      tcatcagat ttgctacaa ctttgcatt tttttcgtc 
      gccctgatt attttgata ttgcaatgc gggagagta 
      cgatcagaa gtcgtaaaa atcttaaat gtgcttaag 
      aaaattgcc ttaaggcgc caaacaacc aatggcgac 
      ttggacgaa cttatcgtc acgctttca actcattat 
      tttccggtt ttcagcgct tgtgattat ttcactatt 
      gaaactaag accgtgttg gatgttttg ggggtcact 
      gtgacctac ctgatcgtt attatacaa tttgacgat 
      aaaatagtg caagtttag gcaaaaaat attcttact 
      ggtcctacg ctcaaataa ttttattcg ctcgcttgt 
      tgatttgtt ttaactgtt taaaacttg ccacaattg 
      tcaagaata aataaatga cgttact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY