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Gene information
General information
TC014998 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4886624..4893334
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014998
    iBeetle screen iB_02374

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    mature egg mostly not present - Number of animals showing the phenotype: 2

    egg chamber potentially not present - Number of animals showing the phenotype: 2

    egg chamber potentially degenerated - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014998 RA
      MATHFNYPD PALQDELRK IANAIVAPG KGILAADES 
      VGTMGKRLA DIGLENTEE NRRKYRQLL FTSDKTIGD 
      YISGVILFH ETLYQKADD GTPFPELLK QRGIIPGIK 
      VDTGVVPLF GTQDECTTQ GLDDLGKRC AQYKKDGCH 
      FAKWRCVLK IKDHTPSYQ ACLENANVL ARYASICQE 
      NRIVPIVEP EILPDGTHD IERCQKVTE TVLAFVYKA 
      LNDHNVFLE GTLLKPNMV TGGMSHPVK FSPEQIGRA 
      TVTALSRTV PPAVPGIVF LSGGQSEEE ATVNLDAIN 
      KFQGKKPWV LSFSYGRAL QASVLRAWG GKDANVPAG 
      QAELMKRAK ANSEASLGK YVAGSVQGQ AADQGLFVK 
      NHAY
    CDS sequence
    • >TC014998 RA
      atggccacc cacttcaac taccccgac cccgccctt 
      caggacgaa ctccgcaaa attgccaac gcgatcgtc 
      gcccccgga aagggcatc ctcgcggcc gacgaatcc 
      gtcggtacg atgggaaag cgcttggcc gacattggc 
      ctggaaaac accgaagaa aaccgacgg aaataccgc 
      cagttgctc ttcaccagc gacaaaaca atcggtgat 
      tacatctcc ggcgtaatc ctcttccat gagactctc 
      taccagaag gccgacgat ggcacccca ttccctgaa 
      ctcctcaaa caaagaggg attattccc gggattaaa 
      gtggacact ggcgttgtc ccactcttc ggcacgcaa 
      gacgagtgc accactcag ggattagac gatttgggc 
      aaacgctgc gcccagtac aagaaggac gggtgccat 
      tttgcaaaa tggcggtgc gtccttaaa atcaaggac 
      cacactccc agctatcag gcgtgtctc gagaatgca 
      aatgttcta gcaagatat gcttcgatt tgtcaggag 
      aatcgtatt gtgcccatt gtggagcca gagatactc 
      cctgatggt acacacgat attgaacgt tgccagaaa 
      gtcacagag acggtcctg gcgttcgtc tacaaggcg 
      ttgaatgac cacaatgtc ttccttgaa ggcacactt 
      cttaagcct aatatggtc acggggggg atgagccac 
      cctgttaag ttctccccc gaacagatt gggagggcc 
      acagtgacg gcgctttcg cgcactgtg cccccagct 
      gtccccgga attgtgttc ttgtcaggg gggcagtcc 
      gaggaggag gccactgtt aacctcgac gccattaac 
      aagtttcag ggcaagaag ccgtgggtt ttgagcttc 
      agttacgga cgggccctc caggcgtcc gttttgagg 
      gcctggggc ggtaaagac gccaatgtg ccagctgga 
      caagccgag ttgatgaag agggccaag gcgaatagt 
      gaggcgtct ttggggaaa tatgtggca gggagtgtg 
      caaggacaa gccgccgat caagggctg tttgtcaag 
      aatcatgct tattaa
    mRNA sequence
    • >TC014998 RA
      ttgctgaca acagttatc gatagtcgt ttgtttatc 
      aaagcgata aagtattca aaatggcca cccacttca 
      actaccccg accccgccc ttcaggacg aactccgca 
      aaattgcca acgcgatcg tcgcccccg gaaagggca 
      tcctcgcgg ccgacgaat ccgtcggta cgatgggaa 
      agcgcttgg ccgacattg gcctggaaa acaccgaag 
      aaaaccgac ggaaatacc gccagttgc tcttcacca 
      gcgacaaaa caatcggtg attacatct ccggcgtaa 
      tcctcttcc atgagactc tctaccaga aggccgacg 
      atggcaccc cattccctg aactcctca aacaaagag 
      ggattattc ccgggatta aagtggaca ctggcgttg 
      tcccactct tcggcacgc aagacgagt gcaccactc 
      agggattag acgatttgg gcaaacgct gcgcccagt 
      acaagaagg acgggtgcc attttgcaa aatggcggt 
      gcgtcctta aaatcaagg accacactc ccagctatc 
      aggcgtgtc tcgagaatg caaatgttc tagcaagat 
      atgcttcga tttgtcagg agaatcgta ttgtgccca 
      ttgtggagc cagagatac tccctgatg gtacacacg 
      atattgaac gttgccaga aagtcacag agacggtcc 
      tggcgttcg tctacaagg cgttgaatg accacaatg 
      tcttccttg aaggcacac ttcttaagc ctaatatgg 
      tcacggggg ggatgagcc accctgtta agttctccc 
      ccgaacaga ttgggaggg ccacagtga cggcgcttt 
      cgcgcactg tgcccccag ctgtccccg gaattgtgt 
      tcttgtcag gggggcagt ccgaggagg aggccactg 
      ttaacctcg acgccatta acaagtttc agggcaaga 
      agccgtggg ttttgagct tcagttacg gacgggccc 
      tccaggcgt ccgttttga gggcctggg gcggtaaag 
      acgccaatg tgccagctg gacaagccg agttgatga 
      agagggcca aggcgaata gtgaggcgt ctttgggga 
      aatatgtgg cagggagtg tgcaaggac aagccgccg 
      atcaagggc tgtttgtca agaatcatg cttattaag 
      ggggctgtc tcgttttat tacacacat gatatattt 
      atatagaaa taaaaatgt tagatattg ttgtcgatt 
      tatatactg tcatttttt atttatatc gtggttgta 
      gattttaaa ggactgaag tcatcgcca tgttttgat 
      tttagcgtc atgtagcgg tttactatg gaaatgtac 
      aagtcggtg gatatttta tgtaacaat gtgtacaat 
      aaaaattta tagtaactt gctgttgtg caaacactg 
      cgtacaggg tacattacg tggtagttg ttgtgtaac 
      atgtttcta ataaattaa cacaaacga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY