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Gene information
General information
TC014986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5341102..5397841
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014986
    iBeetle screen iB_02371

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014986 RA
      MAADMRSPN VSVTPINME PPYPSTPIT NCDLPTGEP 
      QRTRSAPHP GLLTNGDVL DSSDAEVAQ IDFQCAVNM 
      RHKKKRDGA DVPRPMSWE GELSDHERD MCVDDDNGS 
      QVPENLSLS QPKLNTIVN LDSKESPIM RLKQPPMGT 
      YSLHSTPSN SPLLSQRHP VPIIGGPKD LIHKDIMHK 
      ALNVPPTPS PDSAIHSAY SVFSSPNQS PLTQRHITL 
      TPNLSRNNS DASHSSCCS YSSVSVSVS PTQQFSPTH 
      SPIQGRHMH SIHNSPLHH NVLYRSLQE DPSVQYTDH 
      DGLDEAEDK TGLVHTSLP AQPGISRQQ LINSPCPIC 
      GDKISGFHY GIFSCESCK GFFKRTVQN RKNYVCLRG 
      AACPVTVAT RKKCPACRF DKCLQCGMK LEAIREDRT 
      RGGRSTYQC SYSLPPSLT NHAELRASS TDLRQNMGE 
      THHGLTVKL EPSDSSVSH HKRGTLPGS RGVPALLQE 
      IMDVEHLWH YNEVELNKL STEGHLGKE GGSGAVHNL 
      SNHPLLAGT ALAGSTDTN PDFVANLCN IADRRLYKI 
      VKWCKSLPL FKNISIDDQ ICLLINSWC ELLLFSCCF 
      RSMSTPGEI RVSLGKSIT LNQARDMGM QACIERMLN 
      FTEQLRRLR VDQYEYVAM KVIVLLTSD TSELREAEK 
      VRASQEKAL KALQDYTLS HYPDSPAKF GELLLRIPE 
      LQRTCQVGK EMLTIKSKD GDGPSFNLL MELLRGDH
    CDS sequence
    • >TC014986 RA
      atggccgca gacatgagg tctccgaac gtgtccgta 
      actcccatc aacatggag cccccgtat ccatcgacg 
      ccgattaca aattgtgat ttgccaacg ggggaacct 
      cagcgcacc aggagcgcg ccccatcct ggccttctc 
      accaacggc gatgtcctc gacagttcc gacgccgaa 
      gtggcgcaa atcgacttt caatgtgcc gtcaatatg 
      cgacacaaa aagaaaagg gatggggcg gacgtaccc 
      agacccatg tcgtgggaa ggagaactc agcgaccat 
      gagagagat atgtgtgtc gatgatgat aacggatct 
      caggtgccg gagaacttg tcgctgtcg cagcccaaa 
      ctgaacacc atcgtaaac ttggactcg aaagagagt 
      cccatcatg aggctgaaa caaccccca atgggcacc 
      tactccttg cacagcaca cccagcaac tctcccttg 
      ctcagccag cggcatccc gtgccgatc atcggtggt 
      cccaaagac ctcatccac aaggacata atgcacaag 
      gcgttaaac gtccctccg acgccaagt cccgactct 
      gccatccat tcagcgtac agtgtgttc agttcgcca 
      aatcagagc cctttgacg caaagacat atcacctta 
      acgccgaat ttgagtcgg aacaatagt gatgcctcc 
      cattcgagc tgttgtagt tattcgagt gtttcggtt 
      tcggtctcg cccactcaa cagttctca cctactcac 
      agtcctatt cagggccgc catatgcac agtattcat 
      aacagtccc ctacatcac aacgtactc taccgaagc 
      ctccaagag gaccccagt gtacaatac actgaccac 
      gacggtctc gacgaggcc gaagataag actggctta 
      gttcatacg tctctgccc gcacaacct ggaatatca 
      cgtcaacaa ctcatcaac agtccgtgt ccgatatgc 
      ggcgataaa atttcagga tttcattac ggaatattc 
      agttgcgaa tcgtgcaaa ggctttttc aaacgcact 
      gtacaaaat cgaaaaaat tacgtctgt ctaaggggg 
      gctgcgtgt cctgtgact gtagccaca aggaagaag 
      tgtccagcg tgcaggttc gacaagtgt ctgcaatgt 
      ggaatgaaa ctagaagct ataagagaa gacagaact 
      cgaggtggt cgttcgact tatcaatgt tcatattcc 
      cttccaccg tcgttaaca aatcatgcc gaactgagg 
      gcgagttca accgatttg agacagaat atgggtgaa 
      acgcatcac ggtttaacc gttaaattg gaaccatcg 
      gatagttcg gtctctcat cacaagagg ggaactttg 
      ccaggatcg agaggtgtc ccagcatta ttgcaagaa 
      ataatggat gtggagcac ctttggcac tacaacgag 
      gtggagctg aacaaactg agcactgag ggccatctg 
      ggcaaagaa ggaggaagc ggagccgtc cacaatcta 
      tcaaaccat cctcttcta gccggaact gctttggcc 
      ggctcgact gacactaat ccagatttc gtcgcgaat 
      ttgtgcaat atagccgac cgtcgactg tacaaaata 
      gtcaagtgg tgcaaaagc cttccacta ttcaaaaac 
      atatcgatc gacgaccaa atctgtttg ctcataaac 
      tcctggtgc gaattgctc ctgttctcc tgttgtttc 
      cgttccatg tcgactccg ggagagatt cgagtctcc 
      ttaggaaaa agcatcaca ttgaatcag gccagggac 
      atgggaatg caagcgtgt atagagagg atgttgaat 
      tttacggaa cagttgaga aggttgcga gtggatcaa 
      tacgaatat gtggcaatg aaagttatc gttttgctt 
      acatcagat acgtcagag ttgagagag gcggagaaa 
      gttcgagct tcgcaggaa aaagctctc aaagcgctg 
      caagattac acgttgagc cattatccg gacagtcct 
      gccaaattt ggagagttg cttctccgc attcctgaa 
      ttacaacga acttgccag gtggggaaa gaaatgttg 
      acgatcaag tcgaaagat ggcgacggc ccgagcttc 
      aatttgctg atggagctt ctccgaggg gatcactag
    mRNA sequence
    • >TC014986 RA
      accgacaaa gaacccaac cgtccaact gagtgttgc 
      acacgcggc ggtcgcgat agtcaggtt gatgtgcgc 
      caaaccaaa caggtagtg tagtggtac cagcggtcg 
      cttagtggt acctgagac accgtcatc gtcgctctc 
      gtcgtggtt tctctcatc atcatcacc gaaacccca 
      aaatttttc gccaaaaaa cgcgttcta aattaaatt 
      tgttaaatc gtttgacgt ggtgagagt tgtattgtg 
      ctgttgttc atcgtcatc atctacaag agcgccatc 
      caacgtagt ggatctcca acgacgcgg tgccgttgt 
      acttgattc gccgaatga ccttgaggt tgaacacgt 
      gaccaaggg ccttgacct tacctaccc ccaccacac 
      aatttgatg ccgcctttg gtgacaatg cccgatccg 
      tccggaagc gttggagtg agctgatct aggccgtga 
      tggccgcag acatgaggt ctccgaacg tgtccgtaa 
      ctcccatca acatggagc ccccgtatc catcgacgc 
      cgattacaa attgtgatt tgccaacgg gggaacctc 
      agcgcacca ggagcgcgc cccatcctg gccttctca 
      ccaacggcg atgtcctcg acagttccg acgccgaag 
      tggcgcaaa tcgactttc aatgtgccg tcaatatgc 
      gacacaaaa agaaaaggg atggggcgg acgtaccca 
      gacccatgt cgtgggaag gagaactca gcgaccatg 
      agagagata tgtgtgtcg atgatgata acggatctc 
      aggtgccgg agaacttgt cgctgtcgc agcccaaac 
      tgaacacca tcgtaaact tggactcga aagagagtc 
      ccatcatga ggctgaaac aacccccaa tgggcacct 
      actccttgc acagcacac ccagcaact ctcccttgc 
      tcagccagc ggcatcccg tgccgatca tcggtggtc 
      ccaaagacc tcatccaca aggacataa tgcacaagg 
      cgttaaacg tccctccga cgccaagtc ccgactctg 
      ccatccatt cagcgtaca gtgtgttca gttcgccaa 
      atcagagcc ctttgacgc aaagacata tcaccttaa 
      cgccgaatt tgagtcgga acaatagtg atgcctccc 
      attcgagct gttgtagtt attcgagtg tttcggttt 
      cggtctcgc ccactcaac agttctcac ctactcaca 
      gtcctattc agggccgcc atatgcaca gtattcata 
      acagtcccc tacatcaca acgtactct accgaagcc 
      tccaagagg accccagtg tacaataca ctgaccacg 
      acggtctcg acgaggccg aagataaga ctggcttag 
      ttcatacgt ctctgcccg cacaacctg gaatatcac 
      gtcaacaac tcatcaaca gtccgtgtc cgatatgcg 
      gcgataaaa tttcaggat ttcattacg gaatattca 
      gttgcgaat cgtgcaaag gctttttca aacgcactg 
      tacaaaatc gaaaaaatt acgtctgtc taagggggg 
      ctgcgtgtc ctgtgactg tagccacaa ggaagaagt 
      gtccagcgt gcaggttcg acaagtgtc tgcaatgtg 
      gaatgaaac tagaagcta taagagaag acagaactc 
      gaggtggtc gttcgactt atcaatgtt catattccc 
      ttccaccgt cgttaacaa atcatgccg aactgaggg 
      cgagttcaa ccgatttga gacagaata tgggtgaaa 
      cgcatcacg gtttaaccg ttaaattgg aaccatcgg 
      atagttcgg tctctcatc acaagaggg gaactttgc 
      caggatcga gaggtgtcc cagcattat tgcaagaaa 
      taatggatg tggagcacc tttggcact acaacgagg 
      tggagctga acaaactga gcactgagg gccatctgg 
      gcaaagaag gaggaagcg gagccgtcc acaatctat 
      caaaccatc ctcttctag ccggaactg ctttggccg 
      gctcgactg acactaatc cagatttcg tcgcgaatt 
      tgtgcaata tagccgacc gtcgactgt acaaaatag 
      tcaagtggt gcaaaagcc ttccactat tcaaaaaca 
      tatcgatcg acgaccaaa tctgtttgc tcataaact 
      cctggtgcg aattgctcc tgttctcct gttgtttcc 
      gttccatgt cgactccgg gagagattc gagtctcct 
      taggaaaaa gcatcacat tgaatcagg ccagggaca 
      tgggaatgc aagcgtgta tagagagga tgttgaatt 
      ttacggaac agttgagaa ggttgcgag tggatcaat 
      acgaatatg tggcaatga aagttatcg ttttgctta 
      catcagata cgtcagagt tgagagagg cggagaaag 
      ttcgagctt cgcaggaaa aagctctca aagcgctgc 
      aagattaca cgttgagcc attatccgg acagtcctg 
      ccaaatttg gagagttgc ttctccgca ttcctgaat 
      tacaacgaa cttgccagg tggggaaag aaatgttga 
      cgatcaagt cgaaagatg gcgacggcc cgagcttca 
      atttgctga tggagcttc tccgagggg atcactaga 
      tgtgcctta ttagctttg gtggcgggg tcatactta 
      cagacagta ttgcttatt actcttagc tttaagtgg 
      aattttacg tacttagta aacgtgctg gacacccga 
      ttaaaccgt ttccagtat gtctgatat tgagagaga 
      gaaagagaa aaaaaatag ttaagtctt tagttgcag 
      ctgtgtaca gacatgcgc ggaaatttt cttttttat 
      tattataca tacataaag ttagagctt gacgaaaat 
      tgtatatat atttttaaa ttaagttta tattttgat 
      attttttat tatatatta tctgaggct atttattgt 
      gccgaacgt tgacgaatt ttgttatgc tcaagtttt 
      ttacacttg tatttaata cgagtttgg gcatatttt 
      tgcaatttt gcggcgatt tgcaaacct ttccattcc 
      aatgacttg gcagtttga ctgtcaaat gccgtttac 
      acggttttg aacgtactc caaccgcgg cgtaacgca 
      aattacgaa acatgaaca actaaatct aatttacgc 
      tgagcgtta cgctgatga acaaacgca agactattg 
      ttataaata gtctttttt tatttattc gagacgatt 
      ttaaagttt tattttttt agtgccaac atcggttta 
      aattcactt catagtgca agtacctac tcattgttg 
      catgaccat tcaaaattg tttgtttat ttatttgaa 
      aaattgtgt tatttattg tagtttttt cggtattat 
      tgttggctt taaatttta ttaaggtgt ctgataaac 
      tgatcattt agtttggat cattcgtgt catctgaat 
      accagtgtc aatattgaa gaaagaaaa acataagac 
      ttaggggag gccttccat gcctgcatg caatgcagt 
      attagccct aatactttt tgtacttgt cattttact 
      agcaattta tacaaaaca attttgtat tgaaatatt 
      taaatatac tttacaaca aaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY