Status
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Gene information
General information
TC014986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5341102..5397841
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014986
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02371

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014986 RA
      MAADMRSPN VSVTPINME PPYPSTPIT NCDLPTGEP 
QRTRSAPHP GLLTNGDVL DSSDAEVAQ IDFQCAVNM 
RHKKKRDGA DVPRPMSWE GELSDHERD MCVDDDNGS 
QVPENLSLS QPKLNTIVN LDSKESPIM RLKQPPMGT 
YSLHSTPSN SPLLSQRHP VPIIGGPKD LIHKDIMHK 
ALNVPPTPS PDSAIHSAY SVFSSPNQS PLTQRHITL 
TPNLSRNNS DASHSSCCS YSSVSVSVS PTQQFSPTH 
SPIQGRHMH SIHNSPLHH NVLYRSLQE DPSVQYTDH 
DGLDEAEDK TGLVHTSLP AQPGISRQQ LINSPCPIC 
GDKISGFHY GIFSCESCK GFFKRTVQN RKNYVCLRG 
AACPVTVAT RKKCPACRF DKCLQCGMK LEAIREDRT 
RGGRSTYQC SYSLPPSLT NHAELRASS TDLRQNMGE 
THHGLTVKL EPSDSSVSH HKRGTLPGS RGVPALLQE 
IMDVEHLWH YNEVELNKL STEGHLGKE GGSGAVHNL 
SNHPLLAGT ALAGSTDTN PDFVANLCN IADRRLYKI 
VKWCKSLPL FKNISIDDQ ICLLINSWC ELLLFSCCF 
RSMSTPGEI RVSLGKSIT LNQARDMGM QACIERMLN 
FTEQLRRLR VDQYEYVAM KVIVLLTSD TSELREAEK 
VRASQEKAL KALQDYTLS HYPDSPAKF GELLLRIPE 
LQRTCQVGK EMLTIKSKD GDGPSFNLL MELLRGDH
    CDS sequence
    • >TC014986 RA
      atggccgca gacatgagg tctccgaac gtgtccgta 
actcccatc aacatggag cccccgtat ccatcgacg 
ccgattaca aattgtgat ttgccaacg ggggaacct 
cagcgcacc aggagcgcg ccccatcct ggccttctc 
accaacggc gatgtcctc gacagttcc gacgccgaa 
gtggcgcaa atcgacttt caatgtgcc gtcaatatg 
cgacacaaa aagaaaagg gatggggcg gacgtaccc 
agacccatg tcgtgggaa ggagaactc agcgaccat 
gagagagat atgtgtgtc gatgatgat aacggatct 
caggtgccg gagaacttg tcgctgtcg cagcccaaa 
ctgaacacc atcgtaaac ttggactcg aaagagagt 
cccatcatg aggctgaaa caaccccca atgggcacc 
tactccttg cacagcaca cccagcaac tctcccttg 
ctcagccag cggcatccc gtgccgatc atcggtggt 
cccaaagac ctcatccac aaggacata atgcacaag 
gcgttaaac gtccctccg acgccaagt cccgactct 
gccatccat tcagcgtac agtgtgttc agttcgcca 
aatcagagc cctttgacg caaagacat atcacctta 
acgccgaat ttgagtcgg aacaatagt gatgcctcc 
cattcgagc tgttgtagt tattcgagt gtttcggtt 
tcggtctcg cccactcaa cagttctca cctactcac 
agtcctatt cagggccgc catatgcac agtattcat 
aacagtccc ctacatcac aacgtactc taccgaagc 
ctccaagag gaccccagt gtacaatac actgaccac 
gacggtctc gacgaggcc gaagataag actggctta 
gttcatacg tctctgccc gcacaacct ggaatatca 
cgtcaacaa ctcatcaac agtccgtgt ccgatatgc 
ggcgataaa atttcagga tttcattac ggaatattc 
agttgcgaa tcgtgcaaa ggctttttc aaacgcact 
gtacaaaat cgaaaaaat tacgtctgt ctaaggggg 
gctgcgtgt cctgtgact gtagccaca aggaagaag 
tgtccagcg tgcaggttc gacaagtgt ctgcaatgt 
ggaatgaaa ctagaagct ataagagaa gacagaact 
cgaggtggt cgttcgact tatcaatgt tcatattcc 
cttccaccg tcgttaaca aatcatgcc gaactgagg 
gcgagttca accgatttg agacagaat atgggtgaa 
acgcatcac ggtttaacc gttaaattg gaaccatcg 
gatagttcg gtctctcat cacaagagg ggaactttg 
ccaggatcg agaggtgtc ccagcatta ttgcaagaa 
ataatggat gtggagcac ctttggcac tacaacgag 
gtggagctg aacaaactg agcactgag ggccatctg 
ggcaaagaa ggaggaagc ggagccgtc cacaatcta 
tcaaaccat cctcttcta gccggaact gctttggcc 
ggctcgact gacactaat ccagatttc gtcgcgaat 
ttgtgcaat atagccgac cgtcgactg tacaaaata 
gtcaagtgg tgcaaaagc cttccacta ttcaaaaac 
atatcgatc gacgaccaa atctgtttg ctcataaac 
tcctggtgc gaattgctc ctgttctcc tgttgtttc 
cgttccatg tcgactccg ggagagatt cgagtctcc 
ttaggaaaa agcatcaca ttgaatcag gccagggac 
atgggaatg caagcgtgt atagagagg atgttgaat 
tttacggaa cagttgaga aggttgcga gtggatcaa 
tacgaatat gtggcaatg aaagttatc gttttgctt 
acatcagat acgtcagag ttgagagag gcggagaaa 
gttcgagct tcgcaggaa aaagctctc aaagcgctg 
caagattac acgttgagc cattatccg gacagtcct 
gccaaattt ggagagttg cttctccgc attcctgaa 
ttacaacga acttgccag gtggggaaa gaaatgttg 
acgatcaag tcgaaagat ggcgacggc ccgagcttc 
aatttgctg atggagctt ctccgaggg gatcactag
    mRNA sequence
    • >TC014986 RA
      accgacaaa gaacccaac cgtccaact gagtgttgc 
acacgcggc ggtcgcgat agtcaggtt gatgtgcgc 
caaaccaaa caggtagtg tagtggtac cagcggtcg 
cttagtggt acctgagac accgtcatc gtcgctctc 
gtcgtggtt tctctcatc atcatcacc gaaacccca 
aaatttttc gccaaaaaa cgcgttcta aattaaatt 
tgttaaatc gtttgacgt ggtgagagt tgtattgtg 
ctgttgttc atcgtcatc atctacaag agcgccatc 
caacgtagt ggatctcca acgacgcgg tgccgttgt 
acttgattc gccgaatga ccttgaggt tgaacacgt 
gaccaaggg ccttgacct tacctaccc ccaccacac 
aatttgatg ccgcctttg gtgacaatg cccgatccg 
tccggaagc gttggagtg agctgatct aggccgtga 
tggccgcag acatgaggt ctccgaacg tgtccgtaa 
ctcccatca acatggagc ccccgtatc catcgacgc 
cgattacaa attgtgatt tgccaacgg gggaacctc 
agcgcacca ggagcgcgc cccatcctg gccttctca 
ccaacggcg atgtcctcg acagttccg acgccgaag 
tggcgcaaa tcgactttc aatgtgccg tcaatatgc 
gacacaaaa agaaaaggg atggggcgg acgtaccca 
gacccatgt cgtgggaag gagaactca gcgaccatg 
agagagata tgtgtgtcg atgatgata acggatctc 
aggtgccgg agaacttgt cgctgtcgc agcccaaac 
tgaacacca tcgtaaact tggactcga aagagagtc 
ccatcatga ggctgaaac aacccccaa tgggcacct 
actccttgc acagcacac ccagcaact ctcccttgc 
tcagccagc ggcatcccg tgccgatca tcggtggtc 
ccaaagacc tcatccaca aggacataa tgcacaagg 
cgttaaacg tccctccga cgccaagtc ccgactctg 
ccatccatt cagcgtaca gtgtgttca gttcgccaa 
atcagagcc ctttgacgc aaagacata tcaccttaa 
cgccgaatt tgagtcgga acaatagtg atgcctccc 
attcgagct gttgtagtt attcgagtg tttcggttt 
cggtctcgc ccactcaac agttctcac ctactcaca 
gtcctattc agggccgcc atatgcaca gtattcata 
acagtcccc tacatcaca acgtactct accgaagcc 
tccaagagg accccagtg tacaataca ctgaccacg 
acggtctcg acgaggccg aagataaga ctggcttag 
ttcatacgt ctctgcccg cacaacctg gaatatcac 
gtcaacaac tcatcaaca gtccgtgtc cgatatgcg 
gcgataaaa tttcaggat ttcattacg gaatattca 
gttgcgaat cgtgcaaag gctttttca aacgcactg 
tacaaaatc gaaaaaatt acgtctgtc taagggggg 
ctgcgtgtc ctgtgactg tagccacaa ggaagaagt 
gtccagcgt gcaggttcg acaagtgtc tgcaatgtg 
gaatgaaac tagaagcta taagagaag acagaactc 
gaggtggtc gttcgactt atcaatgtt catattccc 
ttccaccgt cgttaacaa atcatgccg aactgaggg 
cgagttcaa ccgatttga gacagaata tgggtgaaa 
cgcatcacg gtttaaccg ttaaattgg aaccatcgg 
atagttcgg tctctcatc acaagaggg gaactttgc 
caggatcga gaggtgtcc cagcattat tgcaagaaa 
taatggatg tggagcacc tttggcact acaacgagg 
tggagctga acaaactga gcactgagg gccatctgg 
gcaaagaag gaggaagcg gagccgtcc acaatctat 
caaaccatc ctcttctag ccggaactg ctttggccg 
gctcgactg acactaatc cagatttcg tcgcgaatt 
tgtgcaata tagccgacc gtcgactgt acaaaatag 
tcaagtggt gcaaaagcc ttccactat tcaaaaaca 
tatcgatcg acgaccaaa tctgtttgc tcataaact 
cctggtgcg aattgctcc tgttctcct gttgtttcc 
gttccatgt cgactccgg gagagattc gagtctcct 
taggaaaaa gcatcacat tgaatcagg ccagggaca 
tgggaatgc aagcgtgta tagagagga tgttgaatt 
ttacggaac agttgagaa ggttgcgag tggatcaat 
acgaatatg tggcaatga aagttatcg ttttgctta 
catcagata cgtcagagt tgagagagg cggagaaag 
ttcgagctt cgcaggaaa aagctctca aagcgctgc 
aagattaca cgttgagcc attatccgg acagtcctg 
ccaaatttg gagagttgc ttctccgca ttcctgaat 
tacaacgaa cttgccagg tggggaaag aaatgttga 
cgatcaagt cgaaagatg gcgacggcc cgagcttca 
atttgctga tggagcttc tccgagggg atcactaga 
tgtgcctta ttagctttg gtggcgggg tcatactta 
cagacagta ttgcttatt actcttagc tttaagtgg 
aattttacg tacttagta aacgtgctg gacacccga 
ttaaaccgt ttccagtat gtctgatat tgagagaga 
gaaagagaa aaaaaatag ttaagtctt tagttgcag 
ctgtgtaca gacatgcgc ggaaatttt cttttttat 
tattataca tacataaag ttagagctt gacgaaaat 
tgtatatat atttttaaa ttaagttta tattttgat 
attttttat tatatatta tctgaggct atttattgt 
gccgaacgt tgacgaatt ttgttatgc tcaagtttt 
ttacacttg tatttaata cgagtttgg gcatatttt 
tgcaatttt gcggcgatt tgcaaacct ttccattcc 
aatgacttg gcagtttga ctgtcaaat gccgtttac 
acggttttg aacgtactc caaccgcgg cgtaacgca 
aattacgaa acatgaaca actaaatct aatttacgc 
tgagcgtta cgctgatga acaaacgca agactattg 
ttataaata gtctttttt tatttattc gagacgatt 
ttaaagttt tattttttt agtgccaac atcggttta 
aattcactt catagtgca agtacctac tcattgttg 
catgaccat tcaaaattg tttgtttat ttatttgaa 
aaattgtgt tatttattg tagtttttt cggtattat 
tgttggctt taaatttta ttaaggtgt ctgataaac 
tgatcattt agtttggat cattcgtgt catctgaat 
accagtgtc aatattgaa gaaagaaaa acataagac 
ttaggggag gccttccat gcctgcatg caatgcagt 
attagccct aatactttt tgtacttgt cattttact 
agcaattta tacaaaaca attttgtat tgaaatatt 
taaatatac tttacaaca aaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY