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Gene information
General information
TC014971 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5701308..5706420
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014971
    iBeetle screen iB_02368

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014971 RA
      MTLNPMCGN VETAQGVPL TVTGVAQCK IMKADELLY 
TASEQFLGK SVKEIKATI LQTLEGHLR AILGTLTVE 
EVYRDRDQF AALVREVAA PDVGRMGIE ILSFTIKDV 
YDDVQYLTS LGKAQTAMV KRDADAGVA EANRDAGIR 
EAECQKSAM DVKYSTDTK IEDNSRMFK LQKANFNQE 
INTAKAEAQ LAYELQAAK IRQKIRNEE IQIDVVERK 
KQIEIEAQE VMRKERELN ATVRLPAEA ESYKVQMIA 
EGKRTQTVQ TAKAESERI KLLGTAEAS AIAGIGKAD 
AERMRQKAA VYKQFGDAA IMSLVIDAL PKIAAEVSA 
PLAKTEEIV LIGGPDTTT AEITRLVGQ IPPAVNALT 
GVNLSQVLG KIPGATTQQ VAAAVK
    CDS sequence
    • >TC014971 RA
      atgacgtta aatcccatg tgcggaaac gtggaaact 
gctcagggg gttccccta acggtcaca ggagtggcc 
cagtgcaag ataatgaaa gctgatgaa ctcctctac 
accgcttcg gagcagttt ctcgggaaa tcagtcaag 
gagatcaaa gcgacgatt ctccagacg ctggaaggg 
cacttgcgt gccatttta ggaactctt acagtcgaa 
gaggtgtac cgcgaccgg gaccaattc gcagccctc 
gtccgggaa gtggcagcc ccggatgtg ggtcggatg 
ggcatcgag atcctctcc ttcaccatc aaggacgtc 
tatgacgac gtccagtac ctcacatcc ctcgggaaa 
gcccagacg gcgatggtg aaacgcgac gccgacgcc 
ggcgtcgcc gaagccaac cgggatgcc ggaatacgc 
gaagccgaa tgccagaaa tcggcaatg gacgtcaag 
tactccacc gacaccaaa atcgaggac aattcacga 
atgttcaag ttgcaaaag gccaacttc aatcaagaa 
attaacact gccaaagcc gaagcacaa ttggcctac 
gaattacaa gccgctaaa attcgccaa aaaattcga 
aacgaagaa attcagatc gacgtggtc gagaggaaa 
aaacaaatc gaaatcgag gctcaagaa gtgatgagg 
aaagagagg gagttgaat gcgacggtg aggcttccg 
gccgaagcc gagagctac aaagtccaa atgatcgcg 
gaagggaag cggactcag actgtgcag acggccaag 
gccgaaagc gagcgcatc aagcttttg ggcacggct 
gaggcctcg gccatcgca ggcatcggc aaggccgac 
gcggagcgc atgcgccag aaagccgcc gtttacaag 
caattcggg gacgccgcc atcatgtct ctcgtcata 
gatgcctta ccaaagatc gcagccgaa gtctccgct 
ccgttggcc aaaaccgag gagattgtt ctgattgga 
ggccctgac acgacgacg gccgagatt acccgactg 
gtgggccag atcccaccc gccgtcaat gccttaacg 
ggagtgaat ctatcccaa gttctcggg aaaatcccc 
ggagctaca acgcaacaa gtcgctgcc gctgtcaaa 
taa
    mRNA sequence
    • >TC014971 RA
      agtcacgat aaacataaa ataatacta tcaaaatcg 
aggccaacc ctttcattg cttccgctc taataattc 
cacaagtcc cacaatatg ccaacaatg acaatatta 
aatttttgc aggaggatg ctgcggttc gaccaagcg 
cgtgacgat cgtaggcgg ctgggcatg ggcctggtg 
gttggtgac ggacgtcca gcgtctgtc cctcgaagt 
gatgacgtt aaatcccat gtgcggaaa cgtggaaac 
tgctcaggg ggttcccct aacggtcac aggagtggc 
ccagtgcaa gataatgaa agctgatga actcctcta 
caccgcttc ggagcagtt tctcgggaa atcagtcaa 
ggagatcaa agcgacgat tctccagac gctggaagg 
gcacttgcg tgccatttt aggaactct tacagtcga 
agaggtgta ccgcgaccg ggaccaatt cgcagccct 
cgtccggga agtggcagc cccggatgt gggtcggat 
gggcatcga gatcctctc cttcaccat caaggacgt 
ctatgacga cgtccagta cctcacatc cctcgggaa 
agcccagac ggcgatggt gaaacgcga cgccgacgc 
cggcgtcgc cgaagccaa ccgggatgc cggaatacg 
cgaagccga atgccagaa atcggcaat ggacgtcaa 
gtactccac cgacaccaa aatcgagga caattcacg 
aatgttcaa gttgcaaaa ggccaactt caatcaaga 
aattaacac tgccaaagc cgaagcaca attggccta 
cgaattaca agccgctaa aattcgcca aaaaattcg 
aaacgaaga aattcagat cgacgtggt cgagaggaa 
aaaacaaat cgaaatcga ggctcaaga agtgatgag 
gaaagagag ggagttgaa tgcgacggt gaggcttcc 
ggccgaagc cgagagcta caaagtcca aatgatcgc 
ggaagggaa gcggactca gactgtgca gacggccaa 
ggccgaaag cgagcgcat caagctttt gggcacggc 
tgaggcctc ggccatcgc aggcatcgg caaggccga 
cgcggagcg catgcgcca gaaagccgc cgtttacaa 
gcaattcgg ggacgccgc catcatgtc tctcgtcat 
agatgcctt accaaagat cgcagccga agtctccgc 
tccgttggc caaaaccga ggagattgt tctgattgg 
aggccctga cacgacgac ggccgagat tacccgact 
ggtgggcca gatcccacc cgccgtcaa tgccttaac 
gggagtgaa tctatccca agttctcgg gaaaatccc 
cggagctac aacgcaaca agtcgctgc cgctgtcaa 
ataataacc ttgcacttt ggctcagga tgttttttt 
tggcgaact cactcgcct gcggctggt gttcataca 
aattcagta gggtagtaa aaaattctc gatgtattt 
ttctactcc cattggacg gatgagtcg gtttttgtt 
tcgggttcg attgaacac caactagta ctccgcttt 
acctttttt attttgtct atattttgt gttgtttta 
ggcttttac actaggtgt gtattgtta ctacccact 
tgtgtgaat aaacatatt ttagtaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY