General information | |||
TC014971 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 5701308..5706420 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014971 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02368 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC014971 RA
MTLNPMCGN VETAQGVPL TVTGVAQCK IMKADELLY TASEQFLGK SVKEIKATI LQTLEGHLR AILGTLTVE EVYRDRDQF AALVREVAA PDVGRMGIE ILSFTIKDV YDDVQYLTS LGKAQTAMV KRDADAGVA EANRDAGIR EAECQKSAM DVKYSTDTK IEDNSRMFK LQKANFNQE INTAKAEAQ LAYELQAAK IRQKIRNEE IQIDVVERK KQIEIEAQE VMRKERELN ATVRLPAEA ESYKVQMIA EGKRTQTVQ TAKAESERI KLLGTAEAS AIAGIGKAD AERMRQKAA VYKQFGDAA IMSLVIDAL PKIAAEVSA PLAKTEEIV LIGGPDTTT AEITRLVGQ IPPAVNALT GVNLSQVLG KIPGATTQQ VAAAVK
- >TC014971 RA
atgacgtta aatcccatg tgcggaaac gtggaaact gctcagggg gttccccta acggtcaca ggagtggcc cagtgcaag ataatgaaa gctgatgaa ctcctctac accgcttcg gagcagttt ctcgggaaa tcagtcaag gagatcaaa gcgacgatt ctccagacg ctggaaggg cacttgcgt gccatttta ggaactctt acagtcgaa gaggtgtac cgcgaccgg gaccaattc gcagccctc gtccgggaa gtggcagcc ccggatgtg ggtcggatg ggcatcgag atcctctcc ttcaccatc aaggacgtc tatgacgac gtccagtac ctcacatcc ctcgggaaa gcccagacg gcgatggtg aaacgcgac gccgacgcc ggcgtcgcc gaagccaac cgggatgcc ggaatacgc gaagccgaa tgccagaaa tcggcaatg gacgtcaag tactccacc gacaccaaa atcgaggac aattcacga atgttcaag ttgcaaaag gccaacttc aatcaagaa attaacact gccaaagcc gaagcacaa ttggcctac gaattacaa gccgctaaa attcgccaa aaaattcga aacgaagaa attcagatc gacgtggtc gagaggaaa aaacaaatc gaaatcgag gctcaagaa gtgatgagg aaagagagg gagttgaat gcgacggtg aggcttccg gccgaagcc gagagctac aaagtccaa atgatcgcg gaagggaag cggactcag actgtgcag acggccaag gccgaaagc gagcgcatc aagcttttg ggcacggct gaggcctcg gccatcgca ggcatcggc aaggccgac gcggagcgc atgcgccag aaagccgcc gtttacaag caattcggg gacgccgcc atcatgtct ctcgtcata gatgcctta ccaaagatc gcagccgaa gtctccgct ccgttggcc aaaaccgag gagattgtt ctgattgga ggccctgac acgacgacg gccgagatt acccgactg gtgggccag atcccaccc gccgtcaat gccttaacg ggagtgaat ctatcccaa gttctcggg aaaatcccc ggagctaca acgcaacaa gtcgctgcc gctgtcaaa taa
- >TC014971 RA
agtcacgat aaacataaa ataatacta tcaaaatcg aggccaacc ctttcattg cttccgctc taataattc cacaagtcc cacaatatg ccaacaatg acaatatta aatttttgc aggaggatg ctgcggttc gaccaagcg cgtgacgat cgtaggcgg ctgggcatg ggcctggtg gttggtgac ggacgtcca gcgtctgtc cctcgaagt gatgacgtt aaatcccat gtgcggaaa cgtggaaac tgctcaggg ggttcccct aacggtcac aggagtggc ccagtgcaa gataatgaa agctgatga actcctcta caccgcttc ggagcagtt tctcgggaa atcagtcaa ggagatcaa agcgacgat tctccagac gctggaagg gcacttgcg tgccatttt aggaactct tacagtcga agaggtgta ccgcgaccg ggaccaatt cgcagccct cgtccggga agtggcagc cccggatgt gggtcggat gggcatcga gatcctctc cttcaccat caaggacgt ctatgacga cgtccagta cctcacatc cctcgggaa agcccagac ggcgatggt gaaacgcga cgccgacgc cggcgtcgc cgaagccaa ccgggatgc cggaatacg cgaagccga atgccagaa atcggcaat ggacgtcaa gtactccac cgacaccaa aatcgagga caattcacg aatgttcaa gttgcaaaa ggccaactt caatcaaga aattaacac tgccaaagc cgaagcaca attggccta cgaattaca agccgctaa aattcgcca aaaaattcg aaacgaaga aattcagat cgacgtggt cgagaggaa aaaacaaat cgaaatcga ggctcaaga agtgatgag gaaagagag ggagttgaa tgcgacggt gaggcttcc ggccgaagc cgagagcta caaagtcca aatgatcgc ggaagggaa gcggactca gactgtgca gacggccaa ggccgaaag cgagcgcat caagctttt gggcacggc tgaggcctc ggccatcgc aggcatcgg caaggccga cgcggagcg catgcgcca gaaagccgc cgtttacaa gcaattcgg ggacgccgc catcatgtc tctcgtcat agatgcctt accaaagat cgcagccga agtctccgc tccgttggc caaaaccga ggagattgt tctgattgg aggccctga cacgacgac ggccgagat tacccgact ggtgggcca gatcccacc cgccgtcaa tgccttaac gggagtgaa tctatccca agttctcgg gaaaatccc cggagctac aacgcaaca agtcgctgc cgctgtcaa ataataacc ttgcacttt ggctcagga tgttttttt tggcgaact cactcgcct gcggctggt gttcataca aattcagta gggtagtaa aaaattctc gatgtattt ttctactcc cattggacg gatgagtcg gtttttgtt tcgggttcg attgaacac caactagta ctccgcttt acctttttt attttgtct atattttgt gttgtttta ggcttttac actaggtgt gtattgtta ctacccact tgtgtgaat aaacatatt ttagtaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
