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Gene information
General information
TC014971 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5701308..5706420
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014971
    iBeetle screen iB_02368

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014971 RA
      MTLNPMCGN VETAQGVPL TVTGVAQCK IMKADELLY 
      TASEQFLGK SVKEIKATI LQTLEGHLR AILGTLTVE 
      EVYRDRDQF AALVREVAA PDVGRMGIE ILSFTIKDV 
      YDDVQYLTS LGKAQTAMV KRDADAGVA EANRDAGIR 
      EAECQKSAM DVKYSTDTK IEDNSRMFK LQKANFNQE 
      INTAKAEAQ LAYELQAAK IRQKIRNEE IQIDVVERK 
      KQIEIEAQE VMRKERELN ATVRLPAEA ESYKVQMIA 
      EGKRTQTVQ TAKAESERI KLLGTAEAS AIAGIGKAD 
      AERMRQKAA VYKQFGDAA IMSLVIDAL PKIAAEVSA 
      PLAKTEEIV LIGGPDTTT AEITRLVGQ IPPAVNALT 
      GVNLSQVLG KIPGATTQQ VAAAVK
    CDS sequence
    • >TC014971 RA
      atgacgtta aatcccatg tgcggaaac gtggaaact 
      gctcagggg gttccccta acggtcaca ggagtggcc 
      cagtgcaag ataatgaaa gctgatgaa ctcctctac 
      accgcttcg gagcagttt ctcgggaaa tcagtcaag 
      gagatcaaa gcgacgatt ctccagacg ctggaaggg 
      cacttgcgt gccatttta ggaactctt acagtcgaa 
      gaggtgtac cgcgaccgg gaccaattc gcagccctc 
      gtccgggaa gtggcagcc ccggatgtg ggtcggatg 
      ggcatcgag atcctctcc ttcaccatc aaggacgtc 
      tatgacgac gtccagtac ctcacatcc ctcgggaaa 
      gcccagacg gcgatggtg aaacgcgac gccgacgcc 
      ggcgtcgcc gaagccaac cgggatgcc ggaatacgc 
      gaagccgaa tgccagaaa tcggcaatg gacgtcaag 
      tactccacc gacaccaaa atcgaggac aattcacga 
      atgttcaag ttgcaaaag gccaacttc aatcaagaa 
      attaacact gccaaagcc gaagcacaa ttggcctac 
      gaattacaa gccgctaaa attcgccaa aaaattcga 
      aacgaagaa attcagatc gacgtggtc gagaggaaa 
      aaacaaatc gaaatcgag gctcaagaa gtgatgagg 
      aaagagagg gagttgaat gcgacggtg aggcttccg 
      gccgaagcc gagagctac aaagtccaa atgatcgcg 
      gaagggaag cggactcag actgtgcag acggccaag 
      gccgaaagc gagcgcatc aagcttttg ggcacggct 
      gaggcctcg gccatcgca ggcatcggc aaggccgac 
      gcggagcgc atgcgccag aaagccgcc gtttacaag 
      caattcggg gacgccgcc atcatgtct ctcgtcata 
      gatgcctta ccaaagatc gcagccgaa gtctccgct 
      ccgttggcc aaaaccgag gagattgtt ctgattgga 
      ggccctgac acgacgacg gccgagatt acccgactg 
      gtgggccag atcccaccc gccgtcaat gccttaacg 
      ggagtgaat ctatcccaa gttctcggg aaaatcccc 
      ggagctaca acgcaacaa gtcgctgcc gctgtcaaa 
      taa
    mRNA sequence
    • >TC014971 RA
      agtcacgat aaacataaa ataatacta tcaaaatcg 
      aggccaacc ctttcattg cttccgctc taataattc 
      cacaagtcc cacaatatg ccaacaatg acaatatta 
      aatttttgc aggaggatg ctgcggttc gaccaagcg 
      cgtgacgat cgtaggcgg ctgggcatg ggcctggtg 
      gttggtgac ggacgtcca gcgtctgtc cctcgaagt 
      gatgacgtt aaatcccat gtgcggaaa cgtggaaac 
      tgctcaggg ggttcccct aacggtcac aggagtggc 
      ccagtgcaa gataatgaa agctgatga actcctcta 
      caccgcttc ggagcagtt tctcgggaa atcagtcaa 
      ggagatcaa agcgacgat tctccagac gctggaagg 
      gcacttgcg tgccatttt aggaactct tacagtcga 
      agaggtgta ccgcgaccg ggaccaatt cgcagccct 
      cgtccggga agtggcagc cccggatgt gggtcggat 
      gggcatcga gatcctctc cttcaccat caaggacgt 
      ctatgacga cgtccagta cctcacatc cctcgggaa 
      agcccagac ggcgatggt gaaacgcga cgccgacgc 
      cggcgtcgc cgaagccaa ccgggatgc cggaatacg 
      cgaagccga atgccagaa atcggcaat ggacgtcaa 
      gtactccac cgacaccaa aatcgagga caattcacg 
      aatgttcaa gttgcaaaa ggccaactt caatcaaga 
      aattaacac tgccaaagc cgaagcaca attggccta 
      cgaattaca agccgctaa aattcgcca aaaaattcg 
      aaacgaaga aattcagat cgacgtggt cgagaggaa 
      aaaacaaat cgaaatcga ggctcaaga agtgatgag 
      gaaagagag ggagttgaa tgcgacggt gaggcttcc 
      ggccgaagc cgagagcta caaagtcca aatgatcgc 
      ggaagggaa gcggactca gactgtgca gacggccaa 
      ggccgaaag cgagcgcat caagctttt gggcacggc 
      tgaggcctc ggccatcgc aggcatcgg caaggccga 
      cgcggagcg catgcgcca gaaagccgc cgtttacaa 
      gcaattcgg ggacgccgc catcatgtc tctcgtcat 
      agatgcctt accaaagat cgcagccga agtctccgc 
      tccgttggc caaaaccga ggagattgt tctgattgg 
      aggccctga cacgacgac ggccgagat tacccgact 
      ggtgggcca gatcccacc cgccgtcaa tgccttaac 
      gggagtgaa tctatccca agttctcgg gaaaatccc 
      cggagctac aacgcaaca agtcgctgc cgctgtcaa 
      ataataacc ttgcacttt ggctcagga tgttttttt 
      tggcgaact cactcgcct gcggctggt gttcataca 
      aattcagta gggtagtaa aaaattctc gatgtattt 
      ttctactcc cattggacg gatgagtcg gtttttgtt 
      tcgggttcg attgaacac caactagta ctccgcttt 
      acctttttt attttgtct atattttgt gttgtttta 
      ggcttttac actaggtgt gtattgtta ctacccact 
      tgtgtgaat aaacatatt ttagtaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY