Status
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Gene information
General information
TC014966 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5815698..5820745
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014966
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02366

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014966 RA
      MDDDLVEDD SPDLFINFT PEVQKAIAE VLPSKDPLD 
      EPDFNSIDY INSLFPTEQ SLSNIDEVV SRMETKIQS 
      IDNEISTVV RGQITASQE GRQSLDEAQ RVIKQLFTH 
      IKTIKEKAE KSEEMVREI TRDIKQLDC AKRNLTLAI 
      TTLNHLHML VGGVDTLKL LTQRRLYGE IALPLQAIS 
      EVMTHFETY SDIPQIKSL SDQVKSIHV ELAEQITHD 
      FKEAFSGTN KTTIPNKQL AQACLVVST LEPKVKKDL 
      LKWFVNLQL QEYNHLFQE TEDTAWLDK IDKRYAWIK 
      RHLLEFEDR LGNLFPQNW EVSERIAVQ FCQNTREEL 
      VKIMAKRRS EIDVKLLLY AIQKTATFE NLLSRRFTG 
      VTLRDDESE PDFGAKPPF TGLIGVCFI PHLEIYIES 
      IDRNLADLI ERFVQDSKQ SQPTEATDT QAAILPSCP 
      DLFVFYKKS MIQCTQLDN GQSMLSLTK TFQKYLHEY 
      SERILQNNL PKIESQSIG TSVQLLTRD LQKISTSGI 
      IQNFSSLLK EGEITRFNK DELAKICCI LSTAEYCLE 
      TTQQLSDKL KEKIEPGLA DQIDLSKEQ DNFHKVISN 
      SIQILVQDL ENACEPALT AMSKIQWQN VDTVGDQSP 
      YITALTTHF KTTIPIIRD NLSHTRKYF TQFCIKFAN 
      SFIPKFIQN IYKCKPINT EGAEQLLLD THMLKTVLL 
      NLPSIASQI NRQAPASYT KVVTKGMTK AEMILKVVM 
      TPVDPSKAF VEQYKKLLP DCQLNDFYK ILDMKSVKR 
      QEQAVLVEV FKSYKT
    CDS sequence
    • >TC014966 RA
      atggacgac gacctggtg gaggacgac agccccgac 
      ctgttcatc aacttcacc cccgaggtg cagaaggcc 
      atcgccgag gtgctaccc agcaaggac cccctcgac 
      gagcccgac ttcaacagt attgactac atcaactcg 
      ctgttcccc accgaacag tccctctcg aatatcgac 
      gaagtcgtg tcccggatg gagaccaaa atccagtcc 
      atcgacaac gagatcagc acggtggtc cgcggccag 
      atcaccgcc agtcaagag gggcgccag tcgctggac 
      gaggcccaa agagtcatc aaacaactc ttcacccac 
      atcaaaaca atcaaggaa aaggcggaa aagtcggaa 
      gagatggtg cgcgaaatc acgcgcgat atcaaacag 
      ctcgactgc gccaaacgc aacctgact ttggccata 
      accaccctc aaccacctc catatgctc gtggggggc 
      gtggacact ttgaaactc ctgacccag agacggctc 
      tacggggaa atcgccctg cctttgcag gccataagc 
      gaggtcatg acccacttt gagacctac tcggacatc 
      ccccaaatc aagagtctg agcgaccag gtcaagtcg 
      atccacgtg gaactggcc gagcaaatc acacacgac 
      ttcaaggag gcgttttcg gggacgaac aagaccacc 
      atccccaac aagcaactc gcacaagcg tgtctcgtg 
      gtttccaca ctcgagccc aaagtcaag aaagacctc 
      ctcaagtgg ttcgtcaat ttgcaattg caggagtac 
      aaccacttg tttcaagag accgaagac accgcctgg 
      ttggacaaa atcgacaag agatacgcc tggatcaag 
      cggcacttg ctcgagttt gaggaccgt ctggggaac 
      ttattcccg caaaactgg gaggtctcc gagcgcatt 
      gccgtgcag ttttgccaa aacacgcgg gaagagctc 
      gttaagatc atggcgaag cgcaggagc gaaatcgac 
      gtgaagttg ctcctctac gccattcag aaaaccgcg 
      acttttgag aacttgctc tcgaggcgc tttacaggc 
      gttacgttg agggacgac gagagtgag cccgatttt 
      ggggcgaaa ccgcctttt accggcctg ataggggtc 
      tgttttatc ccacacttg gagatctac atcgaaagt 
      atcgaccga aacttggcc gatttgatc gaacggttc 
      gttcaagac tccaaacag agccaacca acggaagcg 
      accgacacc caagcggcg attttaccg tcatgtccc 
      gacttattc gtcttttac aagaaaagt atgatccag 
      tgtacgcaa ctcgacaac ggacagtca atgctaagt 
      ctcactaag acgttccaa aagtacttg cacgagtac 
      tccgaacgc atccttcaa aacaatttg cccaaaatc 
      gagagtcag agcattggg acaagtgtc cagttgctc 
      acacgtgac ttgcaaaaa atatccacc tcggggatt 
      atccaaaac ttttcctcc ttgttgaaa gaaggcgag 
      attacgcgg tttaacaag gatgagctg gcgaagatt 
      tgttgtatt ttatcaacg gcggagtat tgtcttgag 
      accacgcag caattgagt gacaaactt aaggagaaa 
      atcgagcca gggttggcc gaccaaatc gatttgtcc 
      aaagaacaa gacaatttt cacaaggtt atttcgaac 
      agtattcaa atcctggtg caggacttg gaaaatgcc 
      tgcgagccc gctttgact gcgatgagt aagatccag 
      tggcagaac gtggacacc gtaggtgac caaagcccc 
      tacattacg gccctaaca acccacttc aagaccacc 
      attcccata attcgggac aacttatcc cacactcgc 
      aaatacttc acccagttt tgcataaaa ttcgccaac 
      tcgttcatc ccgaaattc atccaaaac atttacaaa 
      tgcaaaccg ataaacacc gaaggtgcc gaacaactt 
      ctcctcgac acgcacatg ttgaaaaca gttttacta 
      aacttacct tcgatcgcg tcccaaatc aaccgacaa 
      gcaccagcc tcgtacacc aaagtggtc accaaaggc 
      atgacaaaa gccgagatg atactcaag gtcgtcatg 
      acaccagtt gacccctcc aaagccttc gtcgaacaa 
      tacaagaaa ttgttgccc gattgccag ttgaacgac 
      ttttacaaa attctggac atgaagagt gtgaaaagg 
      caggaacag gcggttttg gtggaggtt ttcaaaagt 
      tacaagact tga
    mRNA sequence
    • >TC014966 RA
      taagaccaa taaaccaaa gtatcacta tctggggcg 
      ctccttaca ctagcacta ctcgtattc gtgtcccca 
      atcgtgtgg tttgtggtg ccagcgcac ctaacctca 
      aaatggacg acgacctgg tggaggacg acagccccg 
      acctgttca tcaacttca cccccgagg tgcagaagg 
      ccatcgccg aggtgctac ccagcaagg accccctcg 
      acgagcccg acttcaaca gtattgact acatcaact 
      cgctgttcc ccaccgaac agtccctct cgaatatcg 
      acgaagtcg tgtcccgga tggagacca aaatccagt 
      ccatcgaca acgagatca gcacggtgg tccgcggcc 
      agatcaccg ccagtcaag aggggcgcc agtcgctgg 
      acgaggccc aaagagtca tcaaacaac tcttcaccc 
      acatcaaaa caatcaagg aaaaggcgg aaaagtcgg 
      aagagatgg tgcgcgaaa tcacgcgcg atatcaaac 
      agctcgact gcgccaaac gcaacctga ctttggcca 
      taaccaccc tcaaccacc tccatatgc tcgtggggg 
      gcgtggaca ctttgaaac tcctgaccc agagacggc 
      tctacgggg aaatcgccc tgcctttgc aggccataa 
      gcgaggtca tgacccact ttgagacct actcggaca 
      tcccccaaa tcaagagtc tgagcgacc aggtcaagt 
      cgatccacg tggaactgg ccgagcaaa tcacacacg 
      acttcaagg aggcgtttt cggggacga acaagacca 
      ccatcccca acaagcaac tcgcacaag cgtgtctcg 
      tggtttcca cactcgagc ccaaagtca agaaagacc 
      tcctcaagt ggttcgtca atttgcaat tgcaggagt 
      acaaccact tgtttcaag agaccgaag acaccgcct 
      ggttggaca aaatcgaca agagatacg cctggatca 
      agcggcact tgctcgagt ttgaggacc gtctgggga 
      acttattcc cgcaaaact gggaggtct ccgagcgca 
      ttgccgtgc agttttgcc aaaacacgc gggaagagc 
      tcgttaaga tcatggcga agcgcagga gcgaaatcg 
      acgtgaagt tgctcctct acgccattc agaaaaccg 
      cgacttttg agaacttgc tctcgaggc gctttacag 
      gcgttacgt tgagggacg acgagagtg agcccgatt 
      ttggggcga aaccgcctt ttaccggcc tgatagggg 
      tctgtttta tcccacact tggagatct acatcgaaa 
      gtatcgacc gaaacttgg ccgatttga tcgaacggt 
      tcgttcaag actccaaac agagccaac caacggaag 
      cgaccgaca cccaagcgg cgattttac cgtcatgtc 
      ccgacttat tcgtctttt acaagaaaa gtatgatcc 
      agtgtacgc aactcgaca acggacagt caatgctaa 
      gtctcacta agacgttcc aaaagtact tgcacgagt 
      actccgaac gcatccttc aaaacaatt tgcccaaaa 
      tcgagagtc agagcattg ggacaagtg tccagttgc 
      tcacacgtg acttgcaaa aaatatcca cctcgggga 
      ttatccaaa acttttcct ccttgttga aagaaggcg 
      agattacgc ggtttaaca aggatgagc tggcgaaga 
      tttgttgta ttttatcaa cggcggagt attgtcttg 
      agaccacgc agcaattga gtgacaaac ttaaggaga 
      aaatcgagc cagggttgg ccgaccaaa tcgatttgt 
      ccaaagaac aagacaatt ttcacaagg ttatttcga 
      acagtattc aaatcctgg tgcaggact tggaaaatg 
      cctgcgagc ccgctttga ctgcgatga gtaagatcc 
      agtggcaga acgtggaca ccgtaggtg accaaagcc 
      cctacatta cggccctaa caacccact tcaagacca 
      ccattccca taattcggg acaacttat cccacactc 
      gcaaatact tcacccagt tttgcataa aattcgcca 
      actcgttca tcccgaaat tcatccaaa acatttaca 
      aatgcaaac cgataaaca ccgaaggtg ccgaacaac 
      ttctcctcg acacgcaca tgttgaaaa cagttttac 
      taaacttac cttcgatcg cgtcccaaa tcaaccgac 
      aagcaccag cctcgtaca ccaaagtgg tcaccaaag 
      gcatgacaa aagccgaga tgatactca aggtcgtca 
      tgacaccag ttgacccct ccaaagcct tcgtcgaac 
      aatacaaga aattgttgc ccgattgcc agttgaacg 
      acttttaca aaattctgg acatgaaga gtgtgaaaa 
      ggcaggaac aggcggttt tggtggagg ttttcaaaa 
      gttacaaga cttgaattt gtaataacg tacttaaac 
      attccatgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY