Status
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Gene information
General information
TC014966 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5815698..5820745
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014966
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02366

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014966 RA
      MDDDLVEDD SPDLFINFT PEVQKAIAE VLPSKDPLD 
EPDFNSIDY INSLFPTEQ SLSNIDEVV SRMETKIQS 
IDNEISTVV RGQITASQE GRQSLDEAQ RVIKQLFTH 
IKTIKEKAE KSEEMVREI TRDIKQLDC AKRNLTLAI 
TTLNHLHML VGGVDTLKL LTQRRLYGE IALPLQAIS 
EVMTHFETY SDIPQIKSL SDQVKSIHV ELAEQITHD 
FKEAFSGTN KTTIPNKQL AQACLVVST LEPKVKKDL 
LKWFVNLQL QEYNHLFQE TEDTAWLDK IDKRYAWIK 
RHLLEFEDR LGNLFPQNW EVSERIAVQ FCQNTREEL 
VKIMAKRRS EIDVKLLLY AIQKTATFE NLLSRRFTG 
VTLRDDESE PDFGAKPPF TGLIGVCFI PHLEIYIES 
IDRNLADLI ERFVQDSKQ SQPTEATDT QAAILPSCP 
DLFVFYKKS MIQCTQLDN GQSMLSLTK TFQKYLHEY 
SERILQNNL PKIESQSIG TSVQLLTRD LQKISTSGI 
IQNFSSLLK EGEITRFNK DELAKICCI LSTAEYCLE 
TTQQLSDKL KEKIEPGLA DQIDLSKEQ DNFHKVISN 
SIQILVQDL ENACEPALT AMSKIQWQN VDTVGDQSP 
YITALTTHF KTTIPIIRD NLSHTRKYF TQFCIKFAN 
SFIPKFIQN IYKCKPINT EGAEQLLLD THMLKTVLL 
NLPSIASQI NRQAPASYT KVVTKGMTK AEMILKVVM 
TPVDPSKAF VEQYKKLLP DCQLNDFYK ILDMKSVKR 
QEQAVLVEV FKSYKT
    CDS sequence
    • >TC014966 RA
      atggacgac gacctggtg gaggacgac agccccgac 
ctgttcatc aacttcacc cccgaggtg cagaaggcc 
atcgccgag gtgctaccc agcaaggac cccctcgac 
gagcccgac ttcaacagt attgactac atcaactcg 
ctgttcccc accgaacag tccctctcg aatatcgac 
gaagtcgtg tcccggatg gagaccaaa atccagtcc 
atcgacaac gagatcagc acggtggtc cgcggccag 
atcaccgcc agtcaagag gggcgccag tcgctggac 
gaggcccaa agagtcatc aaacaactc ttcacccac 
atcaaaaca atcaaggaa aaggcggaa aagtcggaa 
gagatggtg cgcgaaatc acgcgcgat atcaaacag 
ctcgactgc gccaaacgc aacctgact ttggccata 
accaccctc aaccacctc catatgctc gtggggggc 
gtggacact ttgaaactc ctgacccag agacggctc 
tacggggaa atcgccctg cctttgcag gccataagc 
gaggtcatg acccacttt gagacctac tcggacatc 
ccccaaatc aagagtctg agcgaccag gtcaagtcg 
atccacgtg gaactggcc gagcaaatc acacacgac 
ttcaaggag gcgttttcg gggacgaac aagaccacc 
atccccaac aagcaactc gcacaagcg tgtctcgtg 
gtttccaca ctcgagccc aaagtcaag aaagacctc 
ctcaagtgg ttcgtcaat ttgcaattg caggagtac 
aaccacttg tttcaagag accgaagac accgcctgg 
ttggacaaa atcgacaag agatacgcc tggatcaag 
cggcacttg ctcgagttt gaggaccgt ctggggaac 
ttattcccg caaaactgg gaggtctcc gagcgcatt 
gccgtgcag ttttgccaa aacacgcgg gaagagctc 
gttaagatc atggcgaag cgcaggagc gaaatcgac 
gtgaagttg ctcctctac gccattcag aaaaccgcg 
acttttgag aacttgctc tcgaggcgc tttacaggc 
gttacgttg agggacgac gagagtgag cccgatttt 
ggggcgaaa ccgcctttt accggcctg ataggggtc 
tgttttatc ccacacttg gagatctac atcgaaagt 
atcgaccga aacttggcc gatttgatc gaacggttc 
gttcaagac tccaaacag agccaacca acggaagcg 
accgacacc caagcggcg attttaccg tcatgtccc 
gacttattc gtcttttac aagaaaagt atgatccag 
tgtacgcaa ctcgacaac ggacagtca atgctaagt 
ctcactaag acgttccaa aagtacttg cacgagtac 
tccgaacgc atccttcaa aacaatttg cccaaaatc 
gagagtcag agcattggg acaagtgtc cagttgctc 
acacgtgac ttgcaaaaa atatccacc tcggggatt 
atccaaaac ttttcctcc ttgttgaaa gaaggcgag 
attacgcgg tttaacaag gatgagctg gcgaagatt 
tgttgtatt ttatcaacg gcggagtat tgtcttgag 
accacgcag caattgagt gacaaactt aaggagaaa 
atcgagcca gggttggcc gaccaaatc gatttgtcc 
aaagaacaa gacaatttt cacaaggtt atttcgaac 
agtattcaa atcctggtg caggacttg gaaaatgcc 
tgcgagccc gctttgact gcgatgagt aagatccag 
tggcagaac gtggacacc gtaggtgac caaagcccc 
tacattacg gccctaaca acccacttc aagaccacc 
attcccata attcgggac aacttatcc cacactcgc 
aaatacttc acccagttt tgcataaaa ttcgccaac 
tcgttcatc ccgaaattc atccaaaac atttacaaa 
tgcaaaccg ataaacacc gaaggtgcc gaacaactt 
ctcctcgac acgcacatg ttgaaaaca gttttacta 
aacttacct tcgatcgcg tcccaaatc aaccgacaa 
gcaccagcc tcgtacacc aaagtggtc accaaaggc 
atgacaaaa gccgagatg atactcaag gtcgtcatg 
acaccagtt gacccctcc aaagccttc gtcgaacaa 
tacaagaaa ttgttgccc gattgccag ttgaacgac 
ttttacaaa attctggac atgaagagt gtgaaaagg 
caggaacag gcggttttg gtggaggtt ttcaaaagt 
tacaagact tga
    mRNA sequence
    • >TC014966 RA
      taagaccaa taaaccaaa gtatcacta tctggggcg 
ctccttaca ctagcacta ctcgtattc gtgtcccca 
atcgtgtgg tttgtggtg ccagcgcac ctaacctca 
aaatggacg acgacctgg tggaggacg acagccccg 
acctgttca tcaacttca cccccgagg tgcagaagg 
ccatcgccg aggtgctac ccagcaagg accccctcg 
acgagcccg acttcaaca gtattgact acatcaact 
cgctgttcc ccaccgaac agtccctct cgaatatcg 
acgaagtcg tgtcccgga tggagacca aaatccagt 
ccatcgaca acgagatca gcacggtgg tccgcggcc 
agatcaccg ccagtcaag aggggcgcc agtcgctgg 
acgaggccc aaagagtca tcaaacaac tcttcaccc 
acatcaaaa caatcaagg aaaaggcgg aaaagtcgg 
aagagatgg tgcgcgaaa tcacgcgcg atatcaaac 
agctcgact gcgccaaac gcaacctga ctttggcca 
taaccaccc tcaaccacc tccatatgc tcgtggggg 
gcgtggaca ctttgaaac tcctgaccc agagacggc 
tctacgggg aaatcgccc tgcctttgc aggccataa 
gcgaggtca tgacccact ttgagacct actcggaca 
tcccccaaa tcaagagtc tgagcgacc aggtcaagt 
cgatccacg tggaactgg ccgagcaaa tcacacacg 
acttcaagg aggcgtttt cggggacga acaagacca 
ccatcccca acaagcaac tcgcacaag cgtgtctcg 
tggtttcca cactcgagc ccaaagtca agaaagacc 
tcctcaagt ggttcgtca atttgcaat tgcaggagt 
acaaccact tgtttcaag agaccgaag acaccgcct 
ggttggaca aaatcgaca agagatacg cctggatca 
agcggcact tgctcgagt ttgaggacc gtctgggga 
acttattcc cgcaaaact gggaggtct ccgagcgca 
ttgccgtgc agttttgcc aaaacacgc gggaagagc 
tcgttaaga tcatggcga agcgcagga gcgaaatcg 
acgtgaagt tgctcctct acgccattc agaaaaccg 
cgacttttg agaacttgc tctcgaggc gctttacag 
gcgttacgt tgagggacg acgagagtg agcccgatt 
ttggggcga aaccgcctt ttaccggcc tgatagggg 
tctgtttta tcccacact tggagatct acatcgaaa 
gtatcgacc gaaacttgg ccgatttga tcgaacggt 
tcgttcaag actccaaac agagccaac caacggaag 
cgaccgaca cccaagcgg cgattttac cgtcatgtc 
ccgacttat tcgtctttt acaagaaaa gtatgatcc 
agtgtacgc aactcgaca acggacagt caatgctaa 
gtctcacta agacgttcc aaaagtact tgcacgagt 
actccgaac gcatccttc aaaacaatt tgcccaaaa 
tcgagagtc agagcattg ggacaagtg tccagttgc 
tcacacgtg acttgcaaa aaatatcca cctcgggga 
ttatccaaa acttttcct ccttgttga aagaaggcg 
agattacgc ggtttaaca aggatgagc tggcgaaga 
tttgttgta ttttatcaa cggcggagt attgtcttg 
agaccacgc agcaattga gtgacaaac ttaaggaga 
aaatcgagc cagggttgg ccgaccaaa tcgatttgt 
ccaaagaac aagacaatt ttcacaagg ttatttcga 
acagtattc aaatcctgg tgcaggact tggaaaatg 
cctgcgagc ccgctttga ctgcgatga gtaagatcc 
agtggcaga acgtggaca ccgtaggtg accaaagcc 
cctacatta cggccctaa caacccact tcaagacca 
ccattccca taattcggg acaacttat cccacactc 
gcaaatact tcacccagt tttgcataa aattcgcca 
actcgttca tcccgaaat tcatccaaa acatttaca 
aatgcaaac cgataaaca ccgaaggtg ccgaacaac 
ttctcctcg acacgcaca tgttgaaaa cagttttac 
taaacttac cttcgatcg cgtcccaaa tcaaccgac 
aagcaccag cctcgtaca ccaaagtgg tcaccaaag 
gcatgacaa aagccgaga tgatactca aggtcgtca 
tgacaccag ttgacccct ccaaagcct tcgtcgaac 
aatacaaga aattgttgc ccgattgcc agttgaacg 
acttttaca aaattctgg acatgaaga gtgtgaaaa 
ggcaggaac aggcggttt tggtggagg ttttcaaaa 
gttacaaga cttgaattt gtaataacg tacttaaac 
attccatgt
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