General information | |||
TC014966 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 5815698..5820745 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014966 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02366 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014966 RA
MDDDLVEDD SPDLFINFT PEVQKAIAE VLPSKDPLD EPDFNSIDY INSLFPTEQ SLSNIDEVV SRMETKIQS IDNEISTVV RGQITASQE GRQSLDEAQ RVIKQLFTH IKTIKEKAE KSEEMVREI TRDIKQLDC AKRNLTLAI TTLNHLHML VGGVDTLKL LTQRRLYGE IALPLQAIS EVMTHFETY SDIPQIKSL SDQVKSIHV ELAEQITHD FKEAFSGTN KTTIPNKQL AQACLVVST LEPKVKKDL LKWFVNLQL QEYNHLFQE TEDTAWLDK IDKRYAWIK RHLLEFEDR LGNLFPQNW EVSERIAVQ FCQNTREEL VKIMAKRRS EIDVKLLLY AIQKTATFE NLLSRRFTG VTLRDDESE PDFGAKPPF TGLIGVCFI PHLEIYIES IDRNLADLI ERFVQDSKQ SQPTEATDT QAAILPSCP DLFVFYKKS MIQCTQLDN GQSMLSLTK TFQKYLHEY SERILQNNL PKIESQSIG TSVQLLTRD LQKISTSGI IQNFSSLLK EGEITRFNK DELAKICCI LSTAEYCLE TTQQLSDKL KEKIEPGLA DQIDLSKEQ DNFHKVISN SIQILVQDL ENACEPALT AMSKIQWQN VDTVGDQSP YITALTTHF KTTIPIIRD NLSHTRKYF TQFCIKFAN SFIPKFIQN IYKCKPINT EGAEQLLLD THMLKTVLL NLPSIASQI NRQAPASYT KVVTKGMTK AEMILKVVM TPVDPSKAF VEQYKKLLP DCQLNDFYK ILDMKSVKR QEQAVLVEV FKSYKT
- >TC014966 RA
atggacgac gacctggtg gaggacgac agccccgac ctgttcatc aacttcacc cccgaggtg cagaaggcc atcgccgag gtgctaccc agcaaggac cccctcgac gagcccgac ttcaacagt attgactac atcaactcg ctgttcccc accgaacag tccctctcg aatatcgac gaagtcgtg tcccggatg gagaccaaa atccagtcc atcgacaac gagatcagc acggtggtc cgcggccag atcaccgcc agtcaagag gggcgccag tcgctggac gaggcccaa agagtcatc aaacaactc ttcacccac atcaaaaca atcaaggaa aaggcggaa aagtcggaa gagatggtg cgcgaaatc acgcgcgat atcaaacag ctcgactgc gccaaacgc aacctgact ttggccata accaccctc aaccacctc catatgctc gtggggggc gtggacact ttgaaactc ctgacccag agacggctc tacggggaa atcgccctg cctttgcag gccataagc gaggtcatg acccacttt gagacctac tcggacatc ccccaaatc aagagtctg agcgaccag gtcaagtcg atccacgtg gaactggcc gagcaaatc acacacgac ttcaaggag gcgttttcg gggacgaac aagaccacc atccccaac aagcaactc gcacaagcg tgtctcgtg gtttccaca ctcgagccc aaagtcaag aaagacctc ctcaagtgg ttcgtcaat ttgcaattg caggagtac aaccacttg tttcaagag accgaagac accgcctgg ttggacaaa atcgacaag agatacgcc tggatcaag cggcacttg ctcgagttt gaggaccgt ctggggaac ttattcccg caaaactgg gaggtctcc gagcgcatt gccgtgcag ttttgccaa aacacgcgg gaagagctc gttaagatc atggcgaag cgcaggagc gaaatcgac gtgaagttg ctcctctac gccattcag aaaaccgcg acttttgag aacttgctc tcgaggcgc tttacaggc gttacgttg agggacgac gagagtgag cccgatttt ggggcgaaa ccgcctttt accggcctg ataggggtc tgttttatc ccacacttg gagatctac atcgaaagt atcgaccga aacttggcc gatttgatc gaacggttc gttcaagac tccaaacag agccaacca acggaagcg accgacacc caagcggcg attttaccg tcatgtccc gacttattc gtcttttac aagaaaagt atgatccag tgtacgcaa ctcgacaac ggacagtca atgctaagt ctcactaag acgttccaa aagtacttg cacgagtac tccgaacgc atccttcaa aacaatttg cccaaaatc gagagtcag agcattggg acaagtgtc cagttgctc acacgtgac ttgcaaaaa atatccacc tcggggatt atccaaaac ttttcctcc ttgttgaaa gaaggcgag attacgcgg tttaacaag gatgagctg gcgaagatt tgttgtatt ttatcaacg gcggagtat tgtcttgag accacgcag caattgagt gacaaactt aaggagaaa atcgagcca gggttggcc gaccaaatc gatttgtcc aaagaacaa gacaatttt cacaaggtt atttcgaac agtattcaa atcctggtg caggacttg gaaaatgcc tgcgagccc gctttgact gcgatgagt aagatccag tggcagaac gtggacacc gtaggtgac caaagcccc tacattacg gccctaaca acccacttc aagaccacc attcccata attcgggac aacttatcc cacactcgc aaatacttc acccagttt tgcataaaa ttcgccaac tcgttcatc ccgaaattc atccaaaac atttacaaa tgcaaaccg ataaacacc gaaggtgcc gaacaactt ctcctcgac acgcacatg ttgaaaaca gttttacta aacttacct tcgatcgcg tcccaaatc aaccgacaa gcaccagcc tcgtacacc aaagtggtc accaaaggc atgacaaaa gccgagatg atactcaag gtcgtcatg acaccagtt gacccctcc aaagccttc gtcgaacaa tacaagaaa ttgttgccc gattgccag ttgaacgac ttttacaaa attctggac atgaagagt gtgaaaagg caggaacag gcggttttg gtggaggtt ttcaaaagt tacaagact tga
- >TC014966 RA
taagaccaa taaaccaaa gtatcacta tctggggcg ctccttaca ctagcacta ctcgtattc gtgtcccca atcgtgtgg tttgtggtg ccagcgcac ctaacctca aaatggacg acgacctgg tggaggacg acagccccg acctgttca tcaacttca cccccgagg tgcagaagg ccatcgccg aggtgctac ccagcaagg accccctcg acgagcccg acttcaaca gtattgact acatcaact cgctgttcc ccaccgaac agtccctct cgaatatcg acgaagtcg tgtcccgga tggagacca aaatccagt ccatcgaca acgagatca gcacggtgg tccgcggcc agatcaccg ccagtcaag aggggcgcc agtcgctgg acgaggccc aaagagtca tcaaacaac tcttcaccc acatcaaaa caatcaagg aaaaggcgg aaaagtcgg aagagatgg tgcgcgaaa tcacgcgcg atatcaaac agctcgact gcgccaaac gcaacctga ctttggcca taaccaccc tcaaccacc tccatatgc tcgtggggg gcgtggaca ctttgaaac tcctgaccc agagacggc tctacgggg aaatcgccc tgcctttgc aggccataa gcgaggtca tgacccact ttgagacct actcggaca tcccccaaa tcaagagtc tgagcgacc aggtcaagt cgatccacg tggaactgg ccgagcaaa tcacacacg acttcaagg aggcgtttt cggggacga acaagacca ccatcccca acaagcaac tcgcacaag cgtgtctcg tggtttcca cactcgagc ccaaagtca agaaagacc tcctcaagt ggttcgtca atttgcaat tgcaggagt acaaccact tgtttcaag agaccgaag acaccgcct ggttggaca aaatcgaca agagatacg cctggatca agcggcact tgctcgagt ttgaggacc gtctgggga acttattcc cgcaaaact gggaggtct ccgagcgca ttgccgtgc agttttgcc aaaacacgc gggaagagc tcgttaaga tcatggcga agcgcagga gcgaaatcg acgtgaagt tgctcctct acgccattc agaaaaccg cgacttttg agaacttgc tctcgaggc gctttacag gcgttacgt tgagggacg acgagagtg agcccgatt ttggggcga aaccgcctt ttaccggcc tgatagggg tctgtttta tcccacact tggagatct acatcgaaa gtatcgacc gaaacttgg ccgatttga tcgaacggt tcgttcaag actccaaac agagccaac caacggaag cgaccgaca cccaagcgg cgattttac cgtcatgtc ccgacttat tcgtctttt acaagaaaa gtatgatcc agtgtacgc aactcgaca acggacagt caatgctaa gtctcacta agacgttcc aaaagtact tgcacgagt actccgaac gcatccttc aaaacaatt tgcccaaaa tcgagagtc agagcattg ggacaagtg tccagttgc tcacacgtg acttgcaaa aaatatcca cctcgggga ttatccaaa acttttcct ccttgttga aagaaggcg agattacgc ggtttaaca aggatgagc tggcgaaga tttgttgta ttttatcaa cggcggagt attgtcttg agaccacgc agcaattga gtgacaaac ttaaggaga aaatcgagc cagggttgg ccgaccaaa tcgatttgt ccaaagaac aagacaatt ttcacaagg ttatttcga acagtattc aaatcctgg tgcaggact tggaaaatg cctgcgagc ccgctttga ctgcgatga gtaagatcc agtggcaga acgtggaca ccgtaggtg accaaagcc cctacatta cggccctaa caacccact tcaagacca ccattccca taattcggg acaacttat cccacactc gcaaatact tcacccagt tttgcataa aattcgcca actcgttca tcccgaaat tcatccaaa acatttaca aatgcaaac cgataaaca ccgaaggtg ccgaacaac ttctcctcg acacgcaca tgttgaaaa cagttttac taaacttac cttcgatcg cgtcccaaa tcaaccgac aagcaccag cctcgtaca ccaaagtgg tcaccaaag gcatgacaa aagccgaga tgatactca aggtcgtca tgacaccag ttgacccct ccaaagcct tcgtcgaac aatacaaga aattgttgc ccgattgcc agttgaacg acttttaca aaattctgg acatgaaga gtgtgaaaa ggcaggaac aggcggttt tggtggagg ttttcaaaa gttacaaga cttgaattt gtaataacg tacttaaac attccatgt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
