Status
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Gene information
General information
TC014957 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7338236..7344537
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014957
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02365

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014957 RA
      MYRRFVFFL ILGFFPSLR CNPGELALK IIKSTEDSY 
      TPVLSAVVE EKAGANFSL LCDLVSSKS NSNYYDFQI 
      SWIKQSNDL RFWREHDSS DSKKMYSIL KPSEMNKAI 
      KHFYPLKSE DRGKYFCVS LKYNLHKSV EVVVRDDKP 
      PKERPFIQE TIFCSEQMF QCANGFCIF YHYACDGRP 
      DCTDGSDES DEVCHGDPC KDKLQCDDG RCIPTSWCC 
      DRHHDPNCT VTNRPKCCQ VLSDSYEEL EYGSASVTQ 
      SHNGARYLF ITICTLCIL FVILLFLVL ASRVVLMAR 
      KTDIDHSSV NQSNIESIS LHNQTALLS CDVFTYQNN 
      SRLPPSNNH IVDNCDVSD PLLFTEEDQ PPSYIDVIR 
      AQNSGLNLN EPPPPYTSR EVLNVPDDK SETEN
    CDS sequence
    • >TC014957 RA
      atgtatcgc cgtttcgtg tttttccta attttaggt 
      ttttttcct agtttaaga tgtaatcct ggcgaactc 
      gcgttaaaa ataatcaaa agcaccgaa gattcctac 
      actcctgtc ctctcggcg gtcgtggag gagaaggcc 
      ggtgctaat ttttctcta ctctgtgat ttagtgtct 
      tccaaaagc aacagcaac tactatgac tttcaaatc 
      tcctggatt aagcaatcg aacgatttg aggttttgg 
      cgcgagcat gactcctcc gacagcaaa aaaatgtac 
      tcaatcctc aagcccagt gaaatgaat aaagcaatc 
      aagcacttc taccccctc aagtcggag gacaggggc 
      aagtatttc tgcgtcagt ctcaagtac aacctgcac 
      aagagcgtc gaggtggtg gtgcgcgac gacaagccc 
      cccaaggag cggccgttt atccaggaa acgatcttc 
      tgtagcgaa cagatgttt cagtgtgcg aatgggttt 
      tgcattttt tatcactat gcgtgcgac gggaggccc 
      gattgcacc gacggctcg gacgagagc gacgaggtg 
      tgccatggg gacccctgc aaagacaag ctgcagtgc 
      gatgacggc cgctgtata ccgacaagt tggtgttgt 
      gacaggcat catgacccc aactgtacg gtgacaaac 
      cggccgaaa tgttgccag gttttgtcg gattcgtat 
      gaggaattg gaatatggg tcggcttca gtgacgcag 
      agccataat ggggcccga tatctcttt ataacgata 
      tgtactctt tgcatactc ttcgtcatt ttgctcttc 
      ctggtgctc gcctctcgt gtggtcctc atggcccgc 
      aaaaccgac atcgatcac tcaagcgtc aatcagtcg 
      aacatcgaa agcatcagt ttacataat cagacagct 
      ctcctatct tgtgatgtt tttacatat caaaataat 
      tcgcgttta ccgccttct aataaccat atcgtcgac 
      aattgtgat gtttccgat cctttgttg ttcaccgaa 
      gaggatcag ccgccgtcg tacatcgac gttattcgt 
      gctcaaaat tccggattg aatttaaat gaaccgcca 
      ccaccttac accagcaga gaggtgttg aacgttcca 
      gacgataag agcgaaaca gagaactga
    mRNA sequence
    • >TC014957 RA
      tcaacgcca tagaatatg gctgctttt aaataatca 
      aaaaaatgc gttaaaatg tatcgccgt ttcgtgttt 
      ttcctaatt ttaggtttt tttcctagt ttaagatgt 
      aatcctggc gaactcgcg ttaaaaata atcaaaagc 
      accgaagat tcctacact cctgtcctc tcggcggtc 
      gtggaggag aaggccggt gctaatttt tctctactc 
      tgtgattta gtgtcttcc aaaagcaac agcaactac 
      tatgacttt caaatctcc tggattaag caatcgaac 
      gatttgagg ttttggcgc gagcatgac tcctccgac 
      agcaaaaaa atgtactca atcctcaag cccagtgaa 
      atgaataaa gcaatcaag cacttctac cccctcaag 
      tcggaggac aggggcaag tatttctgc gtcagtctc 
      aagtacaac ctgcacaag agcgtcgag gtggtggtg 
      cgcgacgac aagcccccc aaggagcgg ccgtttatc 
      caggaaacg atcttctgt agcgaacag atgtttcag 
      tgtgcgaat gggttttgc attttttat cactatgcg 
      tgcgacggg aggcccgat tgcaccgac ggctcggac 
      gagagcgac gaggtgtgc catggggac ccctgcaaa 
      gacaagctg cagtgcgat gacggccgc tgtataccg 
      acaagttgg tgttgtgac aggcatcat gaccccaac 
      tgtacggtg acaaaccgg ccgaaatgt tgccaggtt 
      ttgtcggat tcgtatgag gaattggaa tatgggtcg 
      gcttcagtg acgcagagc cataatggg gcccgatat 
      ctctttata acgatatgt actctttgc atactcttc 
      gtcattttg ctcttcctg gtgctcgcc tctcgtgtg 
      gtcctcatg gcccgcaaa accgacatc gatcactca 
      agcgtcaat cagtcgaac atcgaaagc atcagttta 
      cataatcag acagctctc ctatcttgt gatgttttt 
      acatatcaa aataattcg cgtttaccg ccttctaat 
      aaccatatc gtcgacaat tgtgatgtt tccgatcct 
      ttgttgttc accgaagag gatcagccg ccgtcgtac 
      atcgacgtt attcgtgct caaaattcc ggattgaat 
      ttaaatgaa ccgccacca ccttacacc agcagagag 
      gtgttgaac gttccagac gataagagc gaaacagag 
      aactgatca cacattcct cctctctgc tgtctcttc 
      ttcctgttg atattaccg tttttccgt tctcgtaat 
      ctccggttt tccatcctg attatccta agtattttt 
      tgtcattga agtatttgt gtttgtact atattttaa 
      tccatgtat ataggaaga ctacgtttt agggagcga 
      tatcgaagc gacaatact agactgtat tttaaacta 
      gtacaaact gattttaat caaaattat atattttag 
      tgaataaaa aatgcaaac aacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY