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Gene information
General information
TC014954 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid43: 2124739..2138105
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014954
    iBeetle screen iB_02364
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014954 RA
      MKGREKLCA KLSSAFLRK WWFVVAASV GIILFGVIV 
      IIFFSSWVN LVINHEVTL RNGSQSFGW WAKPPVVPM 
      IKVYIYNVT NADEFLNNG SKPVVDELG PYVYVEKWE 
      KKNIKFHDN GTVSFNQEK IYTFDESLS AGLEDDVVV 
      VPNIPMLSA TSQSKHAAR FLRLAMASI MDILKIKPF 
      VEVSVGQLL WGYEDPLLK LAKDVVPKE QKLPYEEFG 
      LMYNKNGTS KDTVTLFTG AADITKYGL IDKFNGQSH 
      LTHYTSDKC NSLMGSDGS IFPPHLTKN STIYVYDKD 
      LCRILPLVY EKTVKGSDD IPAFRFTPP LNVFGDVDE 
      NPENLCFCP HGPPCAPSG FFNVSLCQY DSPILLSFP 
      HFYLANDSY RTALDGITP PEPEKHKFY IDVQPVMGT 
      AMSAKARVQ INLAVSQVV DIKQVATFP DIIFPIMWF 
      EEGLDGLPK EMTGLMGLA TTVPPIARA ALAGVLFVM 
      GAVLLLIAV WRLVRGANR LSSLHLAPG HVGPPTNKN 
      KDVPMGNVP RN
    CDS sequence
    • >TC014954 RA
      atgaaagga agagagaaa ctttgtgca aaactcagc 
      agcgctttt ctgcgaaag tggtggttc gtagtggcg 
      gcatctgtt gggattata ctgtttggg gtcattgtt 
      attattttc ttctcgagt tgggtgaat ttagtcata 
      aatcacgaa gtcacgttg cggaatggt tctcaatct 
      tttggatgg tgggcaaag ccacccgtt gtcccaatg 
      atcaaagtc tacatttac aacgtcacc aacgctgat 
      gagttcctc aacaatggg tcgaaacct gtcgtggac 
      gagttggga ccttacgtt tacgtcgag aaatgggag 
      aagaaaaac atcaaattc cacgataac ggcactgtt 
      tccttcaac caggaaaaa atctacacg ttcgacgag 
      tctttatcc gctggcctt gaagatgac gtcgtcgtc 
      gtacccaac atacccatg ctgagtgct acatctcaa 
      tcgaagcat gccgcgaga ttccttcga ctggcaatg 
      gccagtatc atggacatc ttgaagatc aaacctttc 
      gtcgaggtg tcagtgggg caactgttg tggggctac 
      gaggacccc cttctcaaa ctggccaaa gacgtggtc 
      cccaaagag caaaaactg ccctacgag gagttcggt 
      cttatgtac aataaaaat gggacgtcc aaagataca 
      gtgaccctg tttacgggc gcggccgac atcacaaaa 
      tacggactg attgataaa tttaatgga cagtcccat 
      ctgacccat tacacgtcc gataagtgt aacagttta 
      atgggttcc gatgggtcg attttcccg ccacatttg 
      accaaaaac agcaccatt tacgtgtac gataaggac 
      ttgtgccgg attttgcct ctggtatat gagaaaacg 
      gtcaaaggc agcgacgac atccccgcc ttccgcttc 
      accccgccc ctcaacgtc ttcggggac gtggacgaa 
      aaccccgaa aatctgtgt ttctgtcca catggaccg 
      ccctgcgcc ccctccggc tttttcaac gtgtcgctt 
      tgccaatac gattcgccc attttactc tccttcccc 
      catttctac ctagccaat gacagctat cgcaccgcc 
      ctcgacggc atcaccccg cccgagccg gaaaaacac 
      aagttttac atcgacgtg caaccggtc atgggtact 
      gcgatgagc gccaaagct cgcgtccaa atcaactta 
      gccgtgtcg caagtggtt gatatcaaa caagtggcc 
      actttcccc gacattatt ttccccatt atgtggttt 
      gaggagggg cttgacggt ttaccgaag gaaatgacc 
      ggtttgatg ggcttggct acgacggtc ccgcccatt 
      gcgagggcg gcgcttgct ggcgttttg tttgttatg 
      ggggcggtg cttctccta atcgccgtc tggaggtta 
      gtccgaggg gccaatcgg ctcagctcg ttgcatcta 
      gcaccaggt cacgtggga ccgcccacc aataaaaac 
      aaagacgtt cccatggga aacgtacct aggaattaa
    mRNA sequence
    • >TC014954 RA
      ttcagcttt cactccatc atgtcgaaa ccctatccg 
      aaattccac aaggtcaca acttgtgaa aaatccacg 
      ttgtttgag gagggggtc cccccttgc gaaaacaac 
      caagggggg atctagacg gcatacctg aagacttga 
      tcaggtgta acgtgccgt tagcctctc agttggccg 
      tggttactc gatacgcgc gttgcgctt ttatttcac 
      tacacggca tcgcctccc gtttttttt ttcgtgtgt 
      ttccaccac tgacaagtg acgaccgaa gtggatgac 
      gaaaacggg ccaacccgg attaaacaa aacatgatg 
      gtgaagtta taagtggca gtgttaccc ttaagtgta 
      gtgacatca catcaccta cttgatagt ctagacttt 
      taaaaaaat taaaaacaa aatgaaagg aagagagaa 
      actttgtgc aaaactcag cagcgcttt tctgcgaaa 
      gtggtggtt cgtagtggc ggcatctgt tgggattat 
      actgtttgg ggtcattgt tattatttt cttctcgag 
      ttgggtgaa tttagtcat aaatcacga agtcacgtt 
      gcggaatgg ttctcaatc ttttggatg gtgggcaaa 
      gccacccgt tgtcccaat gatcaaagt ctacattta 
      caacgtcac caacgctga tgagttcct caacaatgg 
      gtcgaaacc tgtcgtgga cgagttggg accttacgt 
      ttacgtcga gaaatggga gaagaaaaa catcaaatt 
      ccacgataa cggcactgt ttccttcaa ccaggaaaa 
      aatctacac gttcgacga gtctttatc cgctggcct 
      tgaagatga cgtcgtcgt cgtacccaa catacccat 
      gctgagtgc tacatctca atcgaagca tgccgcgag 
      attccttcg actggcaat ggccagtat catggacat 
      cttgaagat caaaccttt cgtcgaggt gtcagtggg 
      gcaactgtt gtggggcta cgaggaccc ccttctcaa 
      actggccaa agacgtggt ccccaaaga gcaaaaact 
      gccctacga ggagttcgg tcttatgta caataaaaa 
      tgggacgtc caaagatac agtgaccct gtttacggg 
      cgcggccga catcacaaa atacggact gattgataa 
      atttaatgg acagtccca tctgaccca ttacacgtc 
      cgataagtg taacagttt aatgggttc cgatgggtc 
      gattttccc gccacattt gaccaaaaa cagcaccat 
      ttacgtgta cgataagga cttgtgccg gattttgcc 
      tctggtata tgagaaaac ggtcaaagg cagcgacga 
      catccccgc cttccgctt caccccgcc cctcaacgt 
      cttcgggga cgtggacga aaaccccga aaatctgtg 
      tttctgtcc acatggacc gccctgcgc cccctccgg 
      ctttttcaa cgtgtcgct ttgccaata cgattcgcc 
      cattttact ctccttccc ccatttcta cctagccaa 
      tgacagcta tcgcaccgc cctcgacgg catcacccc 
      gcccgagcc ggaaaaaca caagtttta catcgacgt 
      gcaaccggt catgggtac tgcgatgag cgccaaagc 
      tcgcgtcca aatcaactt agccgtgtc gcaagtggt 
      tgatatcaa acaagtggc cactttccc cgacattat 
      tttccccat tatgtggtt tgaggaggg gcttgacgg 
      tttaccgaa ggaaatgac cggtttgat gggcttggc 
      tacgacggt cccgcccat tgcgagggc ggcgcttgc 
      tggcgtttt gtttgttat gggggcggt gcttctcct 
      aatcgccgt ctggaggtt agtccgagg ggccaatcg 
      gctcagctc gttgcatct agcaccagg tcacgtggg 
      accgcccac caataaaaa caaagacgt tcccatggg 
      aaacgtacc taggaatta attcgagag gtgattgaa 
      cgaacaacg aagcaatat tttcttgtt ataggttag 
      gacaatttg ctcattacg gttaagtta gttaggtgt 
      tgtatattc tagtacaaa aatttgttg cttggtcga 
      aattgtgcc ttacacgtg atttaaaaa gacaaaatg 
      gcggttttt tgtacattg ttattgtat aaataattt 
      tagaattga ttgttgtag ggaaagtga tttttttat 
      ttgtttaga tttttctat tgagtccgt cccttttaa 
      ctgtatagt ttcgatagt catttttta tttcaactg 
      tattttatt ggttatgtg tatatttgg acaataaaa 
      tgtattttc tacccgagc ttttgaatt tggcgctac 
      agcggtggc gccatctac tgtttaatt aaccggttt 
      attaaaaaa atatataaa ttgaagatt t