| General information | |||
| TC014950 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 7477920..7485217 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014950 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02360 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
- >TC014950 RA
MSRRRQQNR DDEDNGFAE WGGYMEAKK AKLREQYKE TTAKEVEKL SDIFTGVSI LVNGLTNPS SDQLKLLMA QHGGTFHLY QSSTTTHII ASNLPNVKI NKLGSVPIV KPSWITDSI ALNKLLDYK RYLLYTNQT TTQPPLDFP VVAKTASDP HFLEEFYSN SRLHLISTL GAEYKQFIS DLREKPRDF PGRVKLSHV PKTGAVPQT PVVMHIDMD CFFVSVGLR TRPELRGLP VAVTHARRA QLNPNKERE ETVTLNMDR MPEGVELRM IDGRSSMSE VASCSYEAR KCGVKNGMF LGAAAKLCP QIKTIPYDF EGYKEVSMV LYKTLASYT LNIEAVSCD EMYVEVSDL LKSYDLSVE DWANHIRDE IKTATGCPC STGFGSNRL QARLATKKA KPDGSYYLK PEDVESYLA EIALADLPG VGYATLSKL KSLGLETCG DVQVASLGV LQRELGAKM GETLLEQAK GIDRKPLNY THERKSVSA EINYGIRFK TINECYNFL ESLGNEVWS RLNEIKMRA KTVTLKLMI RAADAPLET AKFLGHGLC DSVSKSQTL AHHISDVKI LHREVRTLY DKMNVNYAE LRGLGVQLT KLEKLAPIN PTLSNFLQQ ESKKIQPTG DKTASRGRE LVRPPSGEK VATRGRPRK NSKGQKNSL SNFFKNKKE ITGQNKSVE IKKAEVDLN VLQELPPEI RAEIIKEYN LETPAPKPP LESPETAPP AKKSPFSGL NWPQLKPIL YKWLQSEQK PDNYDVVLL GEHFRQSAI TRDIDWLPT AMNFLYRNF AQMNCCWHK AYHKIVDLV QQGMVARYG GTLLIKRDF DCLNCF
- >TC014950 RA
atgagtcgc agacgccag caaaaccgt gacgatgaa gacaacggt ttcgcagaa tgggggggt tacatggag gcaaaaaaa gccaaactt agggaacaa tacaaggaa accacagcc aaagaagtc gagaaatta tcggacatt ttcaccgga gtctcaatc ctcgtcaac ggcttgaca aaccccagt tccgaccaa ttaaaatta ttgatggcc caacatggg ggcaccttc cacttgtac caaagctcc acaaccacg cacataatt gcctcgaat ttacccaac gtcaagatt aataagttg ggttcggtc ccaatcgtt aaaccctcg tggatcacc gacagcata gccctcaat aaactcctg gactacaaa cgctacctc ctctacacc aatcaaaca acgacacag ccccccttg gacttcccc gtggtagct aaaacagcc tccgacccc cactttctc gaagaattc tactccaat tctcgccta cacctcatc tcaactttg ggggccgaa tacaaacag tttatctcg gacttgcgc gagaaaccc cgggacttc cccggccgt gtcaaactg tcacacgtc cccaaaacg ggggctgtg ccccaaacc cccgtcgtg atgcacatc gacatggac tgtttcttc gtctcggtg gggctgcgc acgcgaccg gagctccgg gggctcccc gtggccgtg acccacgcc cgaagggct caactcaac cccaacaaa gaacgggag gaaactgta accttgaac atggacaga atgccagaa ggggtggaa ttaaggatg atagacggg aggtcgtcc atgtcagaa gttgcaagt tgtagttac gaggccaga aaatgtggg gttaaaaac gggatgttt ctgggggct gcggccaag ctgtgcccc cagattaag acaattccg tacgatttt gagggctat aaggaggtc tcgatggta ctttacaag accttggcc tcctacacc ctcaatatt gaggccgtg agttgtgac gagatgtac gtcgaggtc agtgacctc ctcaaaagc tacgatttg tcggtggaa gactgggcc aatcacatt cgagacgag ataaagacg gcaacaggt tgtccctgt tcgactggg ttcggcagc aatcggctc caagcccga ttggctacg aaaaaagcc aaacctgac ggttcttac tacctcaag cctgaagat gtcgagtcg tatttggcc gaaatcgct ctcgctgat ttaccaggg gtcggttat gcaaccctt agtaaactc aaaagtttg ggtttggag acgtgtggc gacgtccaa gtggcgtcc ttgggggtt ttacagcgt gaattgggg gccaaaatg ggggaaacc ctcctggaa caagcgaag gggattgac cggaaaccc ctcaattac acacacgaa cggaagtca gtctctgcc gaaatcaac tacggaatc cgcttcaaa acaatcaac gagtgttac aattttttg gaaagtttg ggtaatgaa gtctggagc cgtttgaac gaaatcaaa atgcgggcg aaaaccgtg accttgaaa ttgatgatt cgggcggcc gatgcccct ttggaaact gcaaaattt ctcggtcat ggcttgtgc gattccgtg agtaaaagt caaacactt gcgcatcac atctccgat gttaaaata ctccatcgt gaagttaga acactttac gataaaatg aatgttaat tatgccgaa ttgcgcgga cttggggtt cagttaaca aagttggag aagttggcg ccgattaat ccgacgttg agcaatttt ttgcaacaa gaatcgaag aaaattcaa ccaactggt gataaaacc gccagtaga ggacgcgaa cttgtcaga cccccaagt ggggagaaa gtggccacg agggggcgt cctcgaaaa aattcaaag ggacagaag aacagtctt agtaatttt tttaagaat aagaaggag ataacgggg caaaataag tcagtggag atcaagaag gccgaggtg gacctgaac gtcctccag gaactgccg cccgaaatc cgggccgaa atcatcaaa gagtacaat ctggaaacc ccagccccc aagccaccg cttgagagt cctgagaca gccccacca gccaaaaag tcacctttt agtggcctc aattggccc caactcaaa ccaatcctt tacaagtgg ttacaatca gaacaaaaa cccgacaat tatgacgtt gtgctcctt ggcgaacac ttccgacag tcggcgatt acccgcgat attgattgg ttgcccaca gctatgaat tttttgtac cgaaatttc gctcaaatg aactgttgt tggcataaa gcgtatcat aaaatcgtt gatttggtg caacaggga atggtggcg aggtatgga gggactttg ttgattaaa agagatttc gattgttta aactgtttt taa
- >TC014950 RA
ttcatttgt acgctgctg tcttattgt taaccggac caaactcct aaaaaaatt gaaattgcc gccaaaatt aaacatgca tggatctgt agaacctgc attgattaa tttaaatta ttaagtacc acacgtgat ttaaaataa ggatacaat aaaaagaat aatcctaga acctagacg aataaatta ttaaaaact gtgttaaaa attgaattt gaattaaaa acaatgtag agggcgcca cgtgtggta aacaatgtt cttaaaaaa accaaaatt ttacgcttt aaattaatt aacaagggc aaaaaatga gtcgcagac gccagcaaa accgtgacg atgaagaca acggtttcg cagaatggg ggggttaca tggaggcaa aaaaagcca aacttaggg aacaataca aggaaacca cagccaaag aagtcgaga aattatcgg acattttca ccggagtct caatcctcg tcaacggct tgacaaacc ccagttccg accaattaa aattattga tggcccaac atgggggca ccttccact tgtaccaaa gctccacaa ccacgcaca taattgcct cgaatttac ccaacgtca agattaata agttgggtt cggtcccaa tcgttaaac cctcgtgga tcaccgaca gcatagccc tcaataaac tcctggact acaaacgct acctcctct acaccaatc aaacaacga cacagcccc ccttggact tccccgtgg tagctaaaa cagcctccg acccccact ttctcgaag aattctact ccaattctc gcctacacc tcatctcaa ctttggggg ccgaataca aacagttta tctcggact tgcgcgaga aaccccggg acttccccg gccgtgtca aactgtcac acgtcccca aaacggggg ctgtgcccc aaacccccg tcgtgatgc acatcgaca tggactgtt tcttcgtct cggtggggc tgcgcacgc gaccggagc tccgggggc tccccgtgg ccgtgaccc acgcccgaa gggctcaac tcaacccca acaaagaac gggaggaaa ctgtaacct tgaacatgg acagaatgc cagaagggg tggaattaa ggatgatag acgggaggt cgtccatgt cagaagttg caagttgta gttacgagg ccagaaaat gtggggtta aaaacggga tgtttctgg gggctgcgg ccaagctgt gcccccaga ttaagacaa ttccgtacg attttgagg gctataagg aggtctcga tggtacttt acaagacct tggcctcct acaccctca atattgagg ccgtgagtt gtgacgaga tgtacgtcg aggtcagtg acctcctca aaagctacg atttgtcgg tggaagact gggccaatc acattcgag acgagataa agacggcaa caggttgtc cctgttcga ctgggttcg gcagcaatc ggctccaag cccgattgg ctacgaaaa aagccaaac ctgacggtt cttactacc tcaagcctg aagatgtcg agtcgtatt tggccgaaa tcgctctcg ctgatttac caggggtcg gttatgcaa cccttagta aactcaaaa gtttgggtt tggagacgt gtggcgacg tccaagtgg cgtccttgg gggttttac agcgtgaat tgggggcca aaatggggg aaaccctcc tggaacaag cgaagggga ttgaccgga aacccctca attacacac acgaacgga agtcagtct ctgccgaaa tcaactacg gaatccgct tcaaaacaa tcaacgagt gttacaatt ttttggaaa gtttgggta atgaagtct ggagccgtt tgaacgaaa tcaaaatgc gggcgaaaa ccgtgacct tgaaattga tgattcggg cggccgatg cccctttgg aaactgcaa aatttctcg gtcatggct tgtgcgatt ccgtgagta aaagtcaaa cacttgcgc atcacatct ccgatgtta aaatactcc atcgtgaag ttagaacac tttacgata aaatgaatg ttaattatg ccgaattgc gcggacttg gggttcagt taacaaagt tggagaagt tggcgccga ttaatccga cgttgagca attttttgc aacaagaat cgaagaaaa ttcaaccaa ctggtgata aaaccgcca gtagaggac gcgaacttg tcagacccc caagtgggg agaaagtgg ccacgaggg ggcgtcctc gaaaaaatt caaagggac agaagaaca gtcttagta attttttta agaataaga aggagataa cggggcaaa ataagtcag tggagatca agaaggccg aggtggacc tgaacgtcc tccaggaac tgccgcccg aaatccggg ccgaaatca tcaaagagt acaatctgg aaaccccag cccccaagc caccgcttg agagtcctg agacagccc caccagcca aaaagtcac cttttagtg gcctcaatt ggccccaac tcaaaccaa tcctttaca agtggttac aatcagaac aaaaacccg acaattatg acgttgtgc tccttggcg aacacttcc gacagtcgg cgattaccc gcgatattg attggttgc ccacagcta tgaattttt tgtaccgaa atttcgctc aaatgaact gttgttggc ataaagcgt atcataaaa tcgttgatt tggtgcaac agggaatgg tggcgaggt atggaggga ctttgttga ttaaaagag atttcgatt gtttaaact gtttttaat aaatacatt tcataattt cattattta atcacactt atctccgga ttccttttc tcccggttt ttcgctacg ttggcaact gaggtacga gttcatgtc gtaaaacgc aaagtttgt gtaagagtg gtattcttt gtttttgta atcttcgcc aaatgtttc ctcagcgtt ggcgagttt tgtcgggtc taggacgtc aatctcgtc caaaatgtt cggtaattc taaaaaaat
