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Gene information
General information
TC014950 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7477920..7485217
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014950
    iBeetle screen iB_02360

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 2
    wings not closed - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014950 RA
      MSRRRQQNR DDEDNGFAE WGGYMEAKK AKLREQYKE 
      TTAKEVEKL SDIFTGVSI LVNGLTNPS SDQLKLLMA 
      QHGGTFHLY QSSTTTHII ASNLPNVKI NKLGSVPIV 
      KPSWITDSI ALNKLLDYK RYLLYTNQT TTQPPLDFP 
      VVAKTASDP HFLEEFYSN SRLHLISTL GAEYKQFIS 
      DLREKPRDF PGRVKLSHV PKTGAVPQT PVVMHIDMD 
      CFFVSVGLR TRPELRGLP VAVTHARRA QLNPNKERE 
      ETVTLNMDR MPEGVELRM IDGRSSMSE VASCSYEAR 
      KCGVKNGMF LGAAAKLCP QIKTIPYDF EGYKEVSMV 
      LYKTLASYT LNIEAVSCD EMYVEVSDL LKSYDLSVE 
      DWANHIRDE IKTATGCPC STGFGSNRL QARLATKKA 
      KPDGSYYLK PEDVESYLA EIALADLPG VGYATLSKL 
      KSLGLETCG DVQVASLGV LQRELGAKM GETLLEQAK 
      GIDRKPLNY THERKSVSA EINYGIRFK TINECYNFL 
      ESLGNEVWS RLNEIKMRA KTVTLKLMI RAADAPLET 
      AKFLGHGLC DSVSKSQTL AHHISDVKI LHREVRTLY 
      DKMNVNYAE LRGLGVQLT KLEKLAPIN PTLSNFLQQ 
      ESKKIQPTG DKTASRGRE LVRPPSGEK VATRGRPRK 
      NSKGQKNSL SNFFKNKKE ITGQNKSVE IKKAEVDLN 
      VLQELPPEI RAEIIKEYN LETPAPKPP LESPETAPP 
      AKKSPFSGL NWPQLKPIL YKWLQSEQK PDNYDVVLL 
      GEHFRQSAI TRDIDWLPT AMNFLYRNF AQMNCCWHK 
      AYHKIVDLV QQGMVARYG GTLLIKRDF DCLNCF
    CDS sequence
    • >TC014950 RA
      atgagtcgc agacgccag caaaaccgt gacgatgaa 
      gacaacggt ttcgcagaa tgggggggt tacatggag 
      gcaaaaaaa gccaaactt agggaacaa tacaaggaa 
      accacagcc aaagaagtc gagaaatta tcggacatt 
      ttcaccgga gtctcaatc ctcgtcaac ggcttgaca 
      aaccccagt tccgaccaa ttaaaatta ttgatggcc 
      caacatggg ggcaccttc cacttgtac caaagctcc 
      acaaccacg cacataatt gcctcgaat ttacccaac 
      gtcaagatt aataagttg ggttcggtc ccaatcgtt 
      aaaccctcg tggatcacc gacagcata gccctcaat 
      aaactcctg gactacaaa cgctacctc ctctacacc 
      aatcaaaca acgacacag ccccccttg gacttcccc 
      gtggtagct aaaacagcc tccgacccc cactttctc 
      gaagaattc tactccaat tctcgccta cacctcatc 
      tcaactttg ggggccgaa tacaaacag tttatctcg 
      gacttgcgc gagaaaccc cgggacttc cccggccgt 
      gtcaaactg tcacacgtc cccaaaacg ggggctgtg 
      ccccaaacc cccgtcgtg atgcacatc gacatggac 
      tgtttcttc gtctcggtg gggctgcgc acgcgaccg 
      gagctccgg gggctcccc gtggccgtg acccacgcc 
      cgaagggct caactcaac cccaacaaa gaacgggag 
      gaaactgta accttgaac atggacaga atgccagaa 
      ggggtggaa ttaaggatg atagacggg aggtcgtcc 
      atgtcagaa gttgcaagt tgtagttac gaggccaga 
      aaatgtggg gttaaaaac gggatgttt ctgggggct 
      gcggccaag ctgtgcccc cagattaag acaattccg 
      tacgatttt gagggctat aaggaggtc tcgatggta 
      ctttacaag accttggcc tcctacacc ctcaatatt 
      gaggccgtg agttgtgac gagatgtac gtcgaggtc 
      agtgacctc ctcaaaagc tacgatttg tcggtggaa 
      gactgggcc aatcacatt cgagacgag ataaagacg 
      gcaacaggt tgtccctgt tcgactggg ttcggcagc 
      aatcggctc caagcccga ttggctacg aaaaaagcc 
      aaacctgac ggttcttac tacctcaag cctgaagat 
      gtcgagtcg tatttggcc gaaatcgct ctcgctgat 
      ttaccaggg gtcggttat gcaaccctt agtaaactc 
      aaaagtttg ggtttggag acgtgtggc gacgtccaa 
      gtggcgtcc ttgggggtt ttacagcgt gaattgggg 
      gccaaaatg ggggaaacc ctcctggaa caagcgaag 
      gggattgac cggaaaccc ctcaattac acacacgaa 
      cggaagtca gtctctgcc gaaatcaac tacggaatc 
      cgcttcaaa acaatcaac gagtgttac aattttttg 
      gaaagtttg ggtaatgaa gtctggagc cgtttgaac 
      gaaatcaaa atgcgggcg aaaaccgtg accttgaaa 
      ttgatgatt cgggcggcc gatgcccct ttggaaact 
      gcaaaattt ctcggtcat ggcttgtgc gattccgtg 
      agtaaaagt caaacactt gcgcatcac atctccgat 
      gttaaaata ctccatcgt gaagttaga acactttac 
      gataaaatg aatgttaat tatgccgaa ttgcgcgga 
      cttggggtt cagttaaca aagttggag aagttggcg 
      ccgattaat ccgacgttg agcaatttt ttgcaacaa 
      gaatcgaag aaaattcaa ccaactggt gataaaacc 
      gccagtaga ggacgcgaa cttgtcaga cccccaagt 
      ggggagaaa gtggccacg agggggcgt cctcgaaaa 
      aattcaaag ggacagaag aacagtctt agtaatttt 
      tttaagaat aagaaggag ataacgggg caaaataag 
      tcagtggag atcaagaag gccgaggtg gacctgaac 
      gtcctccag gaactgccg cccgaaatc cgggccgaa 
      atcatcaaa gagtacaat ctggaaacc ccagccccc 
      aagccaccg cttgagagt cctgagaca gccccacca 
      gccaaaaag tcacctttt agtggcctc aattggccc 
      caactcaaa ccaatcctt tacaagtgg ttacaatca 
      gaacaaaaa cccgacaat tatgacgtt gtgctcctt 
      ggcgaacac ttccgacag tcggcgatt acccgcgat 
      attgattgg ttgcccaca gctatgaat tttttgtac 
      cgaaatttc gctcaaatg aactgttgt tggcataaa 
      gcgtatcat aaaatcgtt gatttggtg caacaggga 
      atggtggcg aggtatgga gggactttg ttgattaaa 
      agagatttc gattgttta aactgtttt taa
    mRNA sequence
    • >TC014950 RA
      ttcatttgt acgctgctg tcttattgt taaccggac 
      caaactcct aaaaaaatt gaaattgcc gccaaaatt 
      aaacatgca tggatctgt agaacctgc attgattaa 
      tttaaatta ttaagtacc acacgtgat ttaaaataa 
      ggatacaat aaaaagaat aatcctaga acctagacg 
      aataaatta ttaaaaact gtgttaaaa attgaattt 
      gaattaaaa acaatgtag agggcgcca cgtgtggta 
      aacaatgtt cttaaaaaa accaaaatt ttacgcttt 
      aaattaatt aacaagggc aaaaaatga gtcgcagac 
      gccagcaaa accgtgacg atgaagaca acggtttcg 
      cagaatggg ggggttaca tggaggcaa aaaaagcca 
      aacttaggg aacaataca aggaaacca cagccaaag 
      aagtcgaga aattatcgg acattttca ccggagtct 
      caatcctcg tcaacggct tgacaaacc ccagttccg 
      accaattaa aattattga tggcccaac atgggggca 
      ccttccact tgtaccaaa gctccacaa ccacgcaca 
      taattgcct cgaatttac ccaacgtca agattaata 
      agttgggtt cggtcccaa tcgttaaac cctcgtgga 
      tcaccgaca gcatagccc tcaataaac tcctggact 
      acaaacgct acctcctct acaccaatc aaacaacga 
      cacagcccc ccttggact tccccgtgg tagctaaaa 
      cagcctccg acccccact ttctcgaag aattctact 
      ccaattctc gcctacacc tcatctcaa ctttggggg 
      ccgaataca aacagttta tctcggact tgcgcgaga 
      aaccccggg acttccccg gccgtgtca aactgtcac 
      acgtcccca aaacggggg ctgtgcccc aaacccccg 
      tcgtgatgc acatcgaca tggactgtt tcttcgtct 
      cggtggggc tgcgcacgc gaccggagc tccgggggc 
      tccccgtgg ccgtgaccc acgcccgaa gggctcaac 
      tcaacccca acaaagaac gggaggaaa ctgtaacct 
      tgaacatgg acagaatgc cagaagggg tggaattaa 
      ggatgatag acgggaggt cgtccatgt cagaagttg 
      caagttgta gttacgagg ccagaaaat gtggggtta 
      aaaacggga tgtttctgg gggctgcgg ccaagctgt 
      gcccccaga ttaagacaa ttccgtacg attttgagg 
      gctataagg aggtctcga tggtacttt acaagacct 
      tggcctcct acaccctca atattgagg ccgtgagtt 
      gtgacgaga tgtacgtcg aggtcagtg acctcctca 
      aaagctacg atttgtcgg tggaagact gggccaatc 
      acattcgag acgagataa agacggcaa caggttgtc 
      cctgttcga ctgggttcg gcagcaatc ggctccaag 
      cccgattgg ctacgaaaa aagccaaac ctgacggtt 
      cttactacc tcaagcctg aagatgtcg agtcgtatt 
      tggccgaaa tcgctctcg ctgatttac caggggtcg 
      gttatgcaa cccttagta aactcaaaa gtttgggtt 
      tggagacgt gtggcgacg tccaagtgg cgtccttgg 
      gggttttac agcgtgaat tgggggcca aaatggggg 
      aaaccctcc tggaacaag cgaagggga ttgaccgga 
      aacccctca attacacac acgaacgga agtcagtct 
      ctgccgaaa tcaactacg gaatccgct tcaaaacaa 
      tcaacgagt gttacaatt ttttggaaa gtttgggta 
      atgaagtct ggagccgtt tgaacgaaa tcaaaatgc 
      gggcgaaaa ccgtgacct tgaaattga tgattcggg 
      cggccgatg cccctttgg aaactgcaa aatttctcg 
      gtcatggct tgtgcgatt ccgtgagta aaagtcaaa 
      cacttgcgc atcacatct ccgatgtta aaatactcc 
      atcgtgaag ttagaacac tttacgata aaatgaatg 
      ttaattatg ccgaattgc gcggacttg gggttcagt 
      taacaaagt tggagaagt tggcgccga ttaatccga 
      cgttgagca attttttgc aacaagaat cgaagaaaa 
      ttcaaccaa ctggtgata aaaccgcca gtagaggac 
      gcgaacttg tcagacccc caagtgggg agaaagtgg 
      ccacgaggg ggcgtcctc gaaaaaatt caaagggac 
      agaagaaca gtcttagta attttttta agaataaga 
      aggagataa cggggcaaa ataagtcag tggagatca 
      agaaggccg aggtggacc tgaacgtcc tccaggaac 
      tgccgcccg aaatccggg ccgaaatca tcaaagagt 
      acaatctgg aaaccccag cccccaagc caccgcttg 
      agagtcctg agacagccc caccagcca aaaagtcac 
      cttttagtg gcctcaatt ggccccaac tcaaaccaa 
      tcctttaca agtggttac aatcagaac aaaaacccg 
      acaattatg acgttgtgc tccttggcg aacacttcc 
      gacagtcgg cgattaccc gcgatattg attggttgc 
      ccacagcta tgaattttt tgtaccgaa atttcgctc 
      aaatgaact gttgttggc ataaagcgt atcataaaa 
      tcgttgatt tggtgcaac agggaatgg tggcgaggt 
      atggaggga ctttgttga ttaaaagag atttcgatt 
      gtttaaact gtttttaat aaatacatt tcataattt 
      cattattta atcacactt atctccgga ttccttttc 
      tcccggttt ttcgctacg ttggcaact gaggtacga 
      gttcatgtc gtaaaacgc aaagtttgt gtaagagtg 
      gtattcttt gtttttgta atcttcgcc aaatgtttc 
      ctcagcgtt ggcgagttt tgtcgggtc taggacgtc 
      aatctcgtc caaaatgtt cggtaattc taaaaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY