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Gene information
General information
TC014942 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7599037..7714995
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014942
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02358

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014942 RA
      MDSSALFVF LIGLVVAQG AQVVVEPRE VLVLPGSNV 
      TVMCRVEVP LQYCRVEIP GMQPLNLNS KLSNSEVAY 
      YGGGLPAGQ CGFRINRVT ERNNGVIKC TLGIETEPT 
      ESIGMVQLI VAKAPRYPE LDLSRGTDS VNIYKINDV 
      LQASCIVKD GRPVANISW YLDNEPLYS NDLSMPTVI 
      DLAKENLQS KVQNLTRVL QASDNGKHL RCVAFHPAY 
      PDGRAETSR QLDVKFAPL PQGEIDKFG YIIGEAGLI 
      TVVVEANPK PIIEWDIGG ERIREREND HTGRLEAEP 
      IKDMGNGRY DVSLRIAAI NKHDTEKTY YLRTYNDMG 
      STDYTIKIS TSPEPEGLE LGIGGIIGI IVSILIILL 
      VVFLLVFAK VTGRWCFSD GATVIDYTT GDSGHHVTV 
      DGVSGDGVD NPHHQVSQE YINGNDVKK DEKKIDTAV
    CDS sequence
    • >TC014942 RA
      atggattcc agcgcactc ttcgttttt ttgatcgga 
      ctggtcgtg gcgcaaggt gcccaagtt gtcgtagag 
      ccccgggaa gtgctagtc ctccctgga agcaatgtc 
      accgtcatg tgtcgagtg gaagtaccc ctccagtac 
      tgtcgtgtg gaaatcccc gggatgcaa cctctaaat 
      ctcaactca aaactgtca aatagtgag gttgcctac 
      tatggggga ggcctgcct gctggacaa tgcgggttt 
      cggataaac agagtgact gagagaaat aacggggtg 
      attaaatgt actttgggg attgaaacg gaaccgacg 
      gagtctatt ggcatggtc caactcatc gtagcgaaa 
      gccccaagg tatccagaa ctggactta agccgaggc 
      actgactcg gtcaacatt tacaaaatc aacgacgtc 
      ctccaggcc tcctgtata gtcaaagat ggccggcct 
      gtagccaac atttcatgg tatcttgat aatgagccg 
      ctctacagc aacgatctc agtatgcca actgtgatc 
      gatttggcc aaagagaat ctccaatca aaagtccag 
      aatttgaca cgtgtgcta caggcgtcc gacaatggc 
      aaacattta cggtgtgtg gctttccat cccgcctac 
      cctgatggt agggccgaa acgtcgcgc caacttgat 
      gttaaattc gccccgttg cctcaaggg gagattgac 
      aagtttggg tatatcata ggggaggct gggcttatc 
      acggtggtg gtcgaggcc aaccctaag cccattatc 
      gagtgggat atcggggga gagaggatt agggagagg 
      gagaatgat cacacgggg cgcttggag gctgagccc 
      atcaaagat atgggaaat ggacgctac gatgttagc 
      ttaaggatc gcagctata aacaaacac gacactgag 
      aagacctac tatttgagg acttacaac gacatggga 
      agtaccgat tacacgatc aaaatttcc acaagtcca 
      gaacccgaa ggtctcgag ctcggcatt ggaggaatc 
      atcggcatc atcgtctcc atcctcatc atcctcctc 
      gttgttttc ctcctggtc ttcgccaaa gtgaccggc 
      aggtggtgc ttcagcgat ggcgccacg gtgatcgac 
      tacacgacg ggcgacagc gggcatcat gtgaccgtg 
      gatggagtg agtggggat ggagttgat aatccgcac 
      caccaggtg tcgcaagag tacatcaac gggaacgat 
      gtgaagaaa gatgagaaa aagattgat acggcggtg 
      taa
    mRNA sequence
    • >TC014942 RA
      tactctatt aaggtggat cgtagtaac ctgtgccgt 
      ggtgacgcg tcaaacaat cgacagtta tactataga 
      tatatattt ttttttcga aaaagtggt gacaagtgt 
      gcatcacaa tgttttgat ttgtttatt gtttggtgg 
      tgtttgtta atgatggat tccagcgca ctcttcgtt 
      tttttgatc ggactggtc gtggcgcaa ggtgcccaa 
      gttgtcgta gagccccgg gaagtgcta gtcctccct 
      ggaagcaat gtcaccgtc atgtgtcga gtggaagta 
      cccctccag tactgtcgt gtggaaatc cccgggatg 
      caacctcta aatctcaac tcaaaactg tcaaatagt 
      gaggttgcc tactatggg ggaggcctg cctgctgga 
      caatgcggg tttcggata aacagagtg actgagaga 
      aataacggg gtgattaaa tgtactttg gggattgaa 
      acggaaccg acggagtct attggcatg gtccaactc 
      atcgtagcg aaagcccca aggtatcca gaactggac 
      ttaagccga ggcactgac tcggtcaac atttacaaa 
      atcaacgac gtcctccag gcctcctgt atagtcaaa 
      gatggccgg cctgtagcc aacatttca tggtatctt 
      gataatgag ccgctctac agcaacgat ctcagtatg 
      ccaactgtg atcgatttg gccaaagag aatctccaa 
      tcaaaagtc cagaatttg acacgtgtg ctacaggcg 
      tccgacaat ggcaaacat ttacggtgt gtggctttc 
      catcccgcc taccctgat ggtagggcc gaaacgtcg 
      cgccaactt gatgttaaa ttcgccccg ttgcctcaa 
      ggggagatt gacaagttt gggtatatc ataggggag 
      gctgggctt atcacggtg gtggtcgag gccaaccct 
      aagcccatt atcgagtgg gatatcggg ggagagagg 
      attagggag agggagaat gatcacacg gggcgcttg 
      gaggctgag cccatcaaa gatatggga aatggacgc 
      tacgatgtt agcttaagg atcgcagct ataaacaaa 
      cacgacact gagaagacc tactatttg aggacttac 
      aacgacatg ggaagtacc gattacacg atcaaaatt 
      tccacaagt ccagaaccc gaaggtctc gagctcggc 
      attggagga atcatcggc atcatcgtc tccatcctc 
      atcatcctc ctcgttgtt ttcctcctg gtcttcgcc 
      aaagtgacc ggcaggtgg tgcttcagc gatggcgcc 
      acggtgatc gactacacg acgggcgac agcgggcat 
      catgtgacc gtggatgga gtgagtggg gatggagtt 
      gataatccg caccaccag gtgtcgcaa gagtacatc 
      aacgggaac gatgtgaag aaagatgag aaaaagatt 
      gatacggcg gtgtaatct agggacttt aatcgaatt 
      ttgtgttgg tacgactgc tgtaggtta gatttcctc 
      ccctgattc tcaatgtgt aaatatcgt cgtgatttt 
      tgtgacaat ggggcgctc tatgccctg taataatac 
      tcaattgtt tcaattttt actgccata ttgcaatca 
      aaaatgagg ggtgagtgc tacaaggtt ggcaacgct 
      gcgcgaaac attccatat atttttcta attttagtg 
      tatatttta ttgtaatat aagacaata attagttta 
      tttttttac gtaacttaa ttctcatca cgtctattt 
      attcgccca ttctcggat tatttatac acatttctt 
      agcaattcg ttcaagtgt taagaacgc tgtttaaaa 
      caattatat atttaagag atgaaaatg atattaatg 
      taaaaattt tgtatgtta tatgtactt aaatattta 
      gaaataagt tgaccgata attataaat tgttttata 
      aataaaagt atatgagag aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY