Status
Please wait ...
Gene information
General information
TC014940 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7757633..7758775
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC014940
      iBeetle screen iB_02357

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014940 RA
        MNFLIEKQV EEKSRIQNE IERLSFKLS 
LLTKSLGQR VLAKNNYDK TIKEIEENY 
SSLVEESGK LLSTITKEV HELEEIMDK 
KIGTEQKLE EKRRSMDEG LEKLEEASN 
GSPANASAE LEENEDQAE ESKDTTDEI 
SMKSEEEIE QKLSPSKID KPPPIKSLT 
NGVKNFNPS IDVELQSYR EAYGRQHTT 
PSPPHSING IPGSPNKRG SLPSKSSRR 
RSGPR
      CDS sequence
      • >TC014940 RA
        atgaatttt ttaatagaa aagcaggta gaagagaaa 
agtcggata caaaacgag atcgaacga ttatcgttt 
aaactgtcg ttgttgacg aaaagtctg ggtcagaga 
gttctagct aagaataac tacgacaag acgatcaaa 
gaaatcgaa gaaaactac tcaagtcta gtcgaagag 
tcgggaaaa ctcctctcg accatcacc aaagaggtg 
cacgagctg gaggagatc atggacaag aaaatcggg 
accgaacaa aagcttgag gagaaacga cggtcgatg 
gatgaaggg ctcgaaaaa cttgaagaa gcttccaac 
ggctccccc gccaatgcc tcagccgaa cttgaagaa 
aacgaagac caggctgaa gaatcaaaa gacacaacc 
gacgaaatc tcgatgaaa agcgaggaa gaaatcgag 
caaaaactc tccccctca aaaatcgac aaaccgccc 
ccaatcaaa tcgctcaca aacggcgtc aagaacttc 
aacccgagc atcgacgtc gagctgcag agctacagg 
gaggcctac ggccgccag cacaccacc ccctcgccc 
ccacactca atcaacggg atcccaggc agccccaac 
aaacggggc tccctgccc agcaagagc agtcgccgc 
agatcgggc ccccgataa
      mRNA sequence
      • >TC014940 RA
        aacgaaacg aaatgaatt ttttaatag aaaagcagg 
tagaagaga aaagtcgga tacaaaacg agatcgaac 
gattatcgt ttaaactgt cgttgttga cgaaaagtc 
tgggtcaga gagttctag ctaagaata actacgaca 
agacgatca aagaaatcg aagaaaact actcaagtc 
tagtcgaag agtcgggaa aactcctct cgaccatca 
ccaaagagg tgcacgagc tggaggaga tcatggaca 
agaaaatcg ggaccgaac aaaagcttg aggagaaac 
gacggtcga tggatgaag ggctcgaaa aacttgaag 
aagcttcca acggctccc ccgccaatg cctcagccg 
aacttgaag aaaacgaag accaggctg aagaatcaa 
aagacacaa ccgacgaaa tctcgatga aaagcgagg 
aagaaatcg agcaaaaac tctccccct caaaaatcg 
acaaaccgc ccccaatca aatcgctca caaacggcg 
tcaagaact tcaacccga gcatcgacg tcgagctgc 
agagctaca gggaggcct acggccgcc agcacacca 
ccccctcgc ccccacact caatcaacg ggatcccag 
gcagcccca acaaacggg gctccctgc ccagcaaga 
gcagtcgcc gcagatcgg gcccccgat aattacctt 
gcaacagca cgatgtcat cacgcccgt cctgcccct 
aaaacacca aattaaacc cactttcaa tttaccccc 
aatacaaac cttgaaaaa gtcggtgat tgtgttagt 
ctcgagtgt gattttggg accgcgacg tttcgggtg 
tggagagca gcaatcgtc aggacgggt gcatgcgaa 
aacggcctt cacgcgacg acctacaac caaaagtgc 
tcagttgct tgtcttgtc atttttgag gttatgtcg 
aataaaatt gtgcttgtc tt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY