Status
Please wait ...
Gene information
General information
TC014936 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7872801..7878256
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014936
    iBeetle screen iB_02356

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head appendages random position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014936 RA
      MASPMFSSS SKKHKKHKS ERKERPDTY TEKPPGLKL 
      ILKVGNQAT PEHTTDYYN LSSYSIHAG DDQIVDQND 
      QCLALGRSH HKKSKKKKK KKDKSKDKD RKHRHHHKE 
      KKRKREEVD YDEENRDFS VGSPSLHRE PRTCVLRQR 
      QERTPVQRM LDQLLGLLE KKDPQQFFA WPVTDNIAP 
      GYSSIITQP MDFSTMRQK IEDNQYDNL QDFNADFKL 
      MCTNAMKYN HVDTIYYKA SKKLLHAGS KMMQPEKLG 
      WILNLIPEL TSEDMGFEI TPELRQNKG GHEDHYDSD 
      HVVEGKKRM PTSKFEAIQ DDLTPEEIL NKSQIAARE 
      ARVKLIARR GAPTMGFLK QRKDGTTNL NILVGGEGV 
      IPGTKKRPV LLGQLCGKL TDGTGQIQG FREDRRNVT 
      KPVKPLYYG AFGSYAPSY DSAFANLSK EESDLVYQT 
      YGSDTAVQY AESVLDFVK DSDYATHLV DSLLDLLTG 
      GDHSKTRRV LDENKKLRE EEEAVKTML EMKPIDAIK 
      VDVDELRSL QDLGIDINF LDNMEEEIK ISEERHELQ 
      QKLDSMCQL LQKLQQTQH QRLSQPPPV HLSQCVPPS 
      DEEMQVAEN ITENLTEIA KRVNPGEVV PVGGVRKAL 
      GVALPDVEV STEVGDLES ELRQFLESE PALTQSPLR 
      DDKTIEEIL ME
    CDS sequence
    • >TC014936 RA
      atggcatcg ccgatgttt tcttcaagc tccaagaag 
      cacaaaaag cacaaatcc gagcgcaag gagcgcccc 
      gacacctac acggagaag ccccccggt ttgaaactc 
      atcctgaaa gtgggcaac caggcgact cctgaacac 
      accaccgat tattacaat ttgagttcg tacagtatt 
      cacgctggt gacgatcaa atcgtcgac cagaacgac 
      cagtgcctg gccttgggg cgcagccac cacaaaaaa 
      agcaaaaag aagaagaag aaaaaagac aagagtaaa 
      gacaaggac cggaaacac cgccaccac cacaaagag 
      aagaaacgg aaacgcgaa gaagtggat tatgacgag 
      gaaaatcgt gatttttca gtaggtagt ccgagtttg 
      catcgggag ccacggacc tgtgtcttg aggcagagg 
      caagagagg acgcccgtt caaaggatg ctagaccag 
      ttgttgggg ctcctggag aaaaaggat cctcagcag 
      ttttttgca tggcctgta actgataat atcgcacct 
      ggttattct tcgattatt acacaaccg atggatttt 
      agcaccatg aggcaaaaa atcgaggat aatcaatac 
      gataattta caagatttt aacgccgat tttaagctg 
      atgtgtacc aacgcaatg aagtacaac cacgtcgat 
      acgatttat tacaaagct agtaaaaaa ttgttacac 
      gctggctcg aagatgatg cagcccgaa aagttgggt 
      tggattttg aatctaatt ccagaactg accagtgag 
      gacatgggt tttgagata acgcctgag ttgaggcag 
      aataaaggg gggcatgag gaccattac gacagtgac 
      catgtggtt gagggtaaa aagcgaatg ccaacgtcg 
      aaatttgag gccattcag gacgacctc actccagag 
      gagatcctc aacaaatcg caaatcgca gccagggag 
      gcgagggtt aaattgatt gcgcgtcgt ggtgccccc 
      acaatgggg ttcctcaag cagcgcaag gacggcacc 
      accaatctc aatatactc gtggggggc gaaggtgta 
      atccctgga acaaaaaaa cgcccagtt cttttgggt 
      caactttgc gggaaatta accgatgga actggtcaa 
      attcagggt ttccgtgaa gacagacgt aatgtcacc 
      aaaccggtg aaacccttg tattatggg gctttcggt 
      tcgtacgcc cccagttat gactcagct tttgccaac 
      ttgagtaaa gaagaaagc gatcttgtt taccaaact 
      tatgggtcc gatacggcc gttcagtac gctgaaagt 
      gtgttagat tttgtcaaa gacagtgat tacgccacc 
      catttggtc gacagtttg cttgattta ctgacaggg 
      ggcgaccac agtaaaacc agacgggtt ttggacgag 
      aataaaaaa ttacgagag gaggaagaa gcggttaaa 
      acgatgctt gaaatgaaa ccgattgat gcgattaaa 
      gtggacgtt gacgaattg aggagtctc caagacttg 
      gggattgac atcaacttc ttggacaat atggaagaa 
      gagattaaa atttcggaa gagcgacac gaattgcaa 
      cagaaactt gactcaatg tgtcagttg ttgcaaaag 
      ttgcagcag acgcaacat cagagattg tcgcagcct 
      ccgcccgtt catttgagt cagtgtgtg cccccttca 
      gatgaggaa atgcaagtg gcggaaaat atcaccgaa 
      aatctgacc gaaatcgcc aagagggtc aatccgggg 
      gaggttgtg ccggtcgga ggggtgagg aaagcccta 
      ggggtggcg ctacccgat gttgaagtc agtacagaa 
      gtgggggat ttggagtcc gaattgagg cagtttttg 
      gagagtgag ccggccctg actcagagt ccgttaagg 
      gatgataaa acgatagag gagatttta atggaatag
    mRNA sequence
    • >TC014936 RA
      aattaatta ttattatca gcttgagtt ggtattgcc 
      agctgtcaa caaaaacaa gtccaccct aacctcact 
      tattcccct tctgttttt gcgttctat gagtgtttt 
      cgagtgttt ggtgcctca aaatggcat cgccgatgt 
      tttcttcaa gctccaaga agcacaaaa agcacaaat 
      ccgagcgca aggagcgcc ccgacacct acacggaga 
      agccccccg gtttgaaac tcatcctga aagtgggca 
      accaggcga ctcctgaac acaccaccg attattaca 
      atttgagtt cgtacagta ttcacgctg gtgacgatc 
      aaatcgtcg accagaacg accagtgcc tggccttgg 
      ggcgcagcc accacaaaa aaagcaaaa agaagaaga 
      agaaaaaag acaagagta aagacaagg accggaaac 
      accgccacc accacaaag agaagaaac ggaaacgcg 
      aagaagtgg attatgacg aggaaaatc gtgattttt 
      cagtaggta gtccgagtt tgcatcggg agccacgga 
      cctgtgtct tgaggcaga ggcaagaga ggacgcccg 
      ttcaaagga tgctagacc agttgttgg ggctcctgg 
      agaaaaagg atcctcagc agttttttg catggcctg 
      taactgata atatcgcac ctggttatt cttcgatta 
      ttacacaac cgatggatt ttagcacca tgaggcaaa 
      aaatcgagg ataatcaat acgataatt tacaagatt 
      ttaacgccg attttaagc tgatgtgta ccaacgcaa 
      tgaagtaca accacgtcg atacgattt attacaaag 
      ctagtaaaa aattgttac acgctggct cgaagatga 
      tgcagcccg aaaagttgg gttggattt tgaatctaa 
      ttccagaac tgaccagtg aggacatgg gttttgaga 
      taacgcctg agttgaggc agaataaag gggggcatg 
      aggaccatt acgacagtg accatgtgg ttgagggta 
      aaaagcgaa tgccaacgt cgaaatttg aggccattc 
      aggacgacc tcactccag aggagatcc tcaacaaat 
      cgcaaatcg cagccaggg aggcgaggg ttaaattga 
      ttgcgcgtc gtggtgccc ccacaatgg ggttcctca 
      agcagcgca aggacggca ccaccaatc tcaatatac 
      tcgtggggg gcgaaggtg taatccctg gaacaaaaa 
      aacgcccag ttcttttgg gtcaacttt gcgggaaat 
      taaccgatg gaactggtc aaattcagg gtttccgtg 
      aagacagac gtaatgtca ccaaaccgg tgaaaccct 
      tgtattatg gggctttcg gttcgtacg cccccagtt 
      atgactcag cttttgcca acttgagta aagaagaaa 
      gcgatcttg tttaccaaa cttatgggt ccgatacgg 
      ccgttcagt acgctgaaa gtgtgttag attttgtca 
      aagacagtg attacgcca cccatttgg tcgacagtt 
      tgcttgatt tactgacag ggggcgacc acagtaaaa 
      ccagacggg ttttggacg agaataaaa aattacgag 
      aggaggaag aagcggtta aaacgatgc ttgaaatga 
      aaccgattg atgcgatta aagtggacg ttgacgaat 
      tgaggagtc tccaagact tggggattg acatcaact 
      tcttggaca atatggaag aagagatta aaatttcgg 
      aagagcgac acgaattgc aacagaaac ttgactcaa 
      tgtgtcagt tgttgcaaa agttgcagc agacgcaac 
      atcagagat tgtcgcagc ctccgcccg ttcatttga 
      gtcagtgtg tgccccctt cagatgagg aaatgcaag 
      tggcggaaa atatcaccg aaaatctga ccgaaatcg 
      ccaagaggg tcaatccgg gggaggttg tgccggtcg 
      gaggggtga ggaaagccc taggggtgg cgctacccg 
      atgttgaag tcagtacag aagtggggg atttggagt 
      ccgaattga ggcagtttt tggagagtg agccggccc 
      tgactcaga gtccgttaa gggatgata aaacgatag 
      aggagattt taatggaat aggttttgt gtggataat 
      aaatggtct atgcctga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY