Status
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Gene information
General information
TC033444
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014933,TC014932
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 7970910..7975601
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033444
    iBeetle screen iB_02355

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033444 RA
      MCEKTKVQT FILTQNEFG QKVYKSQSD KSKSFVIDD 
      EGNMVQYQP PGSFRSVKL ESAGDDFDE ITTDDDSVH 
      LGMINSLES ASDFDDHLM QELDSDLMN VEDTHSSLI 
      MQHMDIQEP LLKLRQLLE QRLGLSLAD YSFYLQGTQ 
      MLENDKNLV EQCVQGQGL VQINVQLQA NLKRINILD 
      VLKPAEDYI HVDELNQSE RCQAPPPKA VVQWQVDLA 
      YKREQDRLK IPHDPMEWS QVHVRHWVQ WAVRQFNLP 
      SIKLSDWSM TGRELYNLT VSDFQKIVP YDPGDIFWT 
      HLELLRKMK VVATKKEDR EEPHVMKQK RLNRVMNNY 
      PSAVTYFEN KAGNNGQIQ LWQFLLELL TSKEYKSVI 
      HWTGNDGEF KLRNPQLVA QLWGERKNK PAMNYEKLS 
      RALRYYYDG DMISKVHGE RFVYKHIKG AKDAARSLL 
      FTKLSRQMK VAVQEGGSA DITKNSKLE NVIEQAKRA 
      NMPTATIQS VLRSCEQDK SNSKSHLIE IKGPGGCII 
      LCEVFTSNL HALKQNITT ILKKHKSKY SDGGGMHLF 
      EEKGVIVVE VDGKGESLL EEATDHAIE AGAEDVKDV 
      GGCLEFTCG ATTLSKVVS GLEKFNYKI QSASVEFIP 
      LKVQELDDE DMKTCASLY EKLEGLPDV VRLSDNVA
    CDS sequence
    • >TC033444 RA
      atgtgcgag aagacaaaa gtgcaaaca ttcatcttg 
      actcagaac gagttcggc cagaaagtc tacaagtca 
      cagtcggac aagtcgaag agttttgtg attgacgat 
      gagggcaat atggtgcag taccagccc cctgggtcg 
      tttcgtagc gtcaaattg gagtcagcg ggtgacgat 
      ttcgacgaa atcacaact gatgacgat tcggttcat 
      ttgggaatg atcaattca cttgaaagt gcgagcgat 
      ttcgacgac catttgatg caagaattg gactcggat 
      ttgatgaac gtcgaggac acacactcg agtctcata 
      atgcaacat atggacata caggagccg ctcctcaaa 
      ctgcgccag ttgctggag caaaggctc ggcttgagc 
      ctcgccgat tattcgttc tatttgcag gggacacaa 
      atgctggaa aatgacaaa aatctcgtg gaacagtgt 
      gtgcaaggc caaggtttg gtccaaatc aatgtccag 
      ttacaagcg aatttgaaa cgaattaat attttggac 
      gtccttaag ccagcagaa gactatatt cacgtcgat 
      gagttgaat caaagtgag cggtgccaa gccccgccc 
      ccgaaagcc gtcgtccaa tggcaggtc gatctggcc 
      tacaagcgt gaacaagac cgccttaaa atccctcac 
      gatccaatg gaatggtca caagtgcac gtccgtcat 
      tgggtgcaa tgggcggtc cgtcaattc aacttgcct 
      agcattaaa ttatctgat tggtcaatg acggggcgt 
      gaattatac aatttgacc gtcagcgat ttccagaaa 
      attgttcca tacgatcct ggtgacatc ttctggacg 
      catcttgaa ttgctacga aaaatgaaa gtcgttgcg 
      acaaaaaaa gaagaccgt gaggaacca catgtcatg 
      aaacaaaaa cgactcaat cgtgtcatg aacaattac 
      ccatcggcg gttacatat ttcgaaaat aaggcggga 
      aataacggc cagattcaa ctgtggcag tttttgtta 
      gagttgttg acgagtaaa gaatataag tcggtgata 
      cattggacg gggaatgac ggagaattt aaattaagg 
      aacccacaa ttagtggcg caattgtgg ggcgagagg 
      aaaaacaag ccagcgatg aattacgag aaattgtca 
      cgcgctttg aggtattat tacgatggg gacatgatt 
      tcaaaagtc catggagag agatttgtt tataagcac 
      attaagggg gctaaagac gccgcacgc tctctcctt 
      tttacgaaa ctaagtcgc cagatgaag gtcgcagtc 
      caagagggt ggttcagcc gatataaca aaaaactca 
      aaactggaa aatgtgatc gaacaagcg aaacgtgcc 
      aatatgccc acggccact attcaaagc gtgttgcga 
      agctgcgaa caagacaag tccaacagc aaaagccat 
      ttaattgag atcaagggc cctgggggt tgtataatc 
      ctgtgtgag gtgtttacg agcaatttg cacgctttg 
      aagcagaac attacgacg attttgaaa aaacacaaa 
      agtaaatac agtgatggg ggcggtatg catttgttt 
      gaggagaag ggggttatt gtggtcgag gtcgatggt 
      aagggggag agtttgttg gaggaggcc acggaccat 
      gccattgaa gctggggct gaggacgtt aaggacgtg 
      gggggttgt ctggaattc acatgtggg gccacaacc 
      cttagtaag gtcgtctcc ggtttggag aaatttaat 
      tataaaatt cagagtgcc agtgttgag tttataccg 
      ctcaaagtg caagaattg gacgatgaa gatatgaaa 
      acttgcgcc agtttgtac gaaaagtta gaggggctg 
      cctgacgta gtgagactc tcagataat gttgcgtaa
    mRNA sequence
    • >TC033444 RA
      acatgagaa taaaaaatg cagaaatga aatgcagtt 
      aagttttaa tttctaata gaaacacat tagattggt 
      caggagatg ttctacaca tgtgatatg gtcagtgta 
      acagcttat tgatgtttt tataaagat ttacaatta 
      ttttaataa tttttcctg gtgtgaact tggctgtag 
      aaaacgaga atggtggag aaatggtcg actgtaatg 
      ttcatggtg tcagtgctg ccctcaccg acatttgtt 
      gacttgtca tttaccaat aataataat aatatgaaa 
      ataattgaa taatcctta ataatgtgc gagaagaca 
      aaagtgcaa acattcatc ttgactcag aacgagttc 
      ggccagaaa gtctacaag tcacagtcg gacaagtcg 
      aagagtttt gtgattgac gatgagggc aatatggtg 
      cagtaccag ccccctggg tcgtttcgt agcgtcaaa 
      ttggagtca gcgggtgac gatttcgac gaaatcaca 
      actgatgac gattcggtt catttggga atgatcaat 
      tcacttgaa agtgcgagc gatttcgac gaccatttg 
      atgcaagaa ttggactcg gatttgatg aacgtcgag 
      gacacacac tcgagtctc ataatgcaa catatggac 
      atacaggag ccgctcctc aaactgcgc cagttgctg 
      gagcaaagg ctcggcttg agcctcgcc gattattcg 
      ttctatttg caggggaca caaatgctg gaaaatgac 
      aaaaatctc gtggaacag tgtgtgcaa ggccaaggt 
      ttggtccaa atcaatgtc cagttacaa gcgaatttg 
      aaacgaatt aatattttg gacgtcctt aagccagca 
      gaagactat attcacgtc gatgagttg aatcaaagt 
      gagcggtgc caagccccg cccccgaaa gccgtcgtc 
      caatggcag gtcgatctg gcctacaag cgtgaacaa 
      gaccgcctt aaaatccct cacgatcca atggaatgg 
      tcacaagtg cacgtccgt cattgggtg caatgggcg 
      gtccgtcaa ttcaacttg cctagcatt aaattatct 
      gattggtca atgacgggg cgtgaatta tacaatttg 
      accgtcagc gatttccag aaaattgtt ccatacgat 
      cctggtgac atcttctgg acgcatctt gaattgcta 
      cgaaaaatg aaagtcgtt gcgacaaaa aaagaagac 
      cgtgaggaa ccacatgtc atgaaacaa aaacgactc 
      aatcgtgtc atgaacaat tacccatcg gcggttaca 
      tatttcgaa aataaggcg ggaaataac ggccagatt 
      caactgtgg cagtttttg ttagagttg ttgacgagt 
      aaagaatat aagtcggtg atacattgg acggggaat 
      gacggagaa tttaaatta aggaaccca caattagtg 
      gcgcaattg tggggcgag aggaaaaac aagccagcg 
      atgaattac gagaaattg tcacgcgct ttgaggtat 
      tattacgat ggggacatg atttcaaaa gtccatgga 
      gagagattt gtttataag cacattaag ggggctaaa 
      gacgccgca cgctctctc ctttttacg aaactaagt 
      cgccagatg aaggtcgca gtccaagag ggtggttca 
      gccgatata acaaaaaac tcaaaactg gaaaatgtg 
      atcgaacaa gcgaaacgt gccaatatg cccacggcc 
      actattcaa agcgtgttg cgaagctgc gaacaagac 
      aagtccaac agcaaaagc catttaatt gagatcaag 
      ggccctggg ggttgtata atcctgtgt gaggtgttt 
      acgagcaat ttgcacgct ttgaagcag aacattacg 
      acgattttg aaaaaacac aaaagtaaa tacagtgat 
      gggggcggt atgcatttg tttgaggag aagggggtt 
      attgtggtc gaggtcgat ggtaagggg gagagtttg 
      ttggaggag gccacggac catgccatt gaagctggg 
      gctgaggac gttaaggac gtggggggt tgtctggaa 
      ttcacatgt ggggccaca acccttagt aaggtcgtc 
      tccggtttg gagaaattt aattataaa attcagagt 
      gccagtgtt gagtttata ccgctcaaa gtgcaagaa 
      ttggacgat gaagatatg aaaacttgc gccagtttg 
      tacgaaaag ttagagggg ctgcctgac gtagtgaga 
      ctctcagat aatgttgcg taaaattat ttattttcc 
      acacaaagt acattaagg atcacagat ttcttaaac 
      aatgtaatt attgtaaat ataaaaaat acacttatt 
      gttactat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY