Status
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Gene information
General information
TC033447
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014921,TC014922
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8032869..8047804
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033447
    iBeetle screen iB_02352

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    iBeetle screen iB_05723

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033447 RA
      METDEGESS SSGPLSSFN SLFSFTSPA VKKLLGWKQ 
      GDEEEKWAE KAVDSLVKK LKKRKGAIE ELERALSCP 
      GTQSNCVTI PRSLDGRLQ VSHRKGLPH VIYCRVWRW 
      PDLQSHHEL KPLEHCKFP FSAKQKEVC INPYHYKRV 
      ESQVLPPVL VPRHSDFVP GHSLLPFQQ VVEPNMPQN 
      ISYNSNGFN SNHLSPSSP LSSVSSPGS VSSNNPQSP 
      YSNLAETPP PAYSPTDEN TNNNTSPQP SLNTDVQPV 
      AYQEQPYWA SIAYYELNC RVGEVFHCH STSVIVDGF 
      TNPSNNSDR FCLGQLSNV NRNSTIENT RRHIGKGVH 
      LYYVNGEVY AECLSDSPI FVQSRNCNH HHGFHPSTV 
      CKIPAGCSL RIFNNAEFA QLLSQCVNH GFEAVYELT 
      KMCTIRMSF VKGWGAEYH RQDVTSTPC WIEVHLNGP 
      LQWLDKVLT QMGSPHNAI SSVSDFKPS AALTELFII 
      QKRRRKQKM VDSCNIGDG EITMVDGNP FKTVKGNIT 
      VVKIEDKFQ IEVNSGGNR QLVIPCEEV ISRSIQNET 
      VILQFKEDN LFEKGICKF YDNRQAIVF NSKLPSIMK 
      RTSTNHNSS TSSDSDFND SGNGKTEGL NNNSFNADT 
      ASDEMEEL
    CDS sequence
    • >TC033447 RA
      atggagacc gacgagggg gagtcctcc agcagcggc 
      cccttgtcc agcttcaac agcctcttc tccttcacc 
      agccccgcg gtgaagaag ctcctcggc tggaagcag 
      ggcgacgag gaggagaag tgggcagag aaggcagtg 
      gacagcctc gtcaagaag ctgaagaag cgcaagggg 
      gccattgag gagctcgag cgcgcgctt tcctgcccc 
      gggacgcag agcaactgc gtgacgata ccccgcagc 
      ctcgacggc cgcctccag gtgtcccac cgcaaaggc 
      ctcccccac gtgatctac tgccgggtc tggcgctgg 
      cccgacctt cagagccac cacgagctg aaacctctg 
      gagcactgc aagttcccg ttttcggcc aagcagaag 
      gaggtctgc atcaacccc taccactac aaaagagtg 
      gaaagtcaa gttttgccg ccggtcttg gtcccccga 
      cacagcgat tttgtccca gggcactcg ctcctaccg 
      tttcagcaa gtggtggag cccaacatg ccccagaat 
      attagttat aattccaat ggatttaat tccaatcat 
      ttgagtccg agttcgcca ctgtcgagt gtgtctagt 
      ccggggagt gtttcgtcg aataatccg cagagtccg 
      tattcgaat ttggcggag actccgccc ccggcctat 
      agccccacc gacgagaat acgaataat aatacgagt 
      ccgcaaccg tcgcttaat acagatgtg caaccggtg 
      gcctatcag gagcagccc tattgggct tctatcgcc 
      tactatgag ttgaattgt cgcgtcggg gaagttttc 
      cattgccat tcgacgtcc gttattgtt gatgggttc 
      accaatccc agcaacaac agcgacaga ttttgctta 
      gggcagcta agcaacgta aaccgcaac tcaaccatc 
      gaaaacacc cgccgtcac atcggcaaa ggggtccac 
      ctatactac gtcaacggc gaagtttac gccgaatgt 
      ctctccgat tcgccaatt ttcgtccaa tcccgcaac 
      tgtaaccac caccatggg tttcaccca tccaccgtg 
      tgtaaaatc ccggctggt tgttcgttg cgtattttc 
      aacaacgcc gaatttgcc caactcctc tcccaatgc 
      gtgaatcat ggttttgag gccgtttac gaactcaca 
      aaaatgtgt acgatacgt atgagcttc gtcaagggt 
      tggggcgcg gagtaccat cgtcaggac gtcacaagc 
      accccctgc tggatcgag gtgcatctc aacggcccc 
      ctccagtgg ctcgacaag gtgctgacc caaatgggg 
      tcccctcac aatgccata agttcggtc tctgatttc 
      aagccatca gctgcgtta accgaactt ttcattatc 
      caaaagaga agaagaaaa cagaaaatg gttgattca 
      tgtaacatt ggagatggt gaaataacc atggttgat 
      ggaaatcca ttcaaaaca gttaaagga aatataaca 
      gttgtgaaa attgaagat aaatttcaa attgaagtc 
      aattctggt ggcaacaga caactagtt attccatgt 
      gaagaagtc atcagtcgc agcatacag aacgaaact 
      gtgatttta caatttaaa gaggataat ctcttcgaa 
      aaaggcatt tgtaaattt tatgataat agacaagca 
      attgttttc aattccaaa ctgccttct attatgaaa 
      cgaacatcc acaaaccac aattcgtct actagttct 
      gactcagac tttaatgac tcaggaaac ggaaaaact 
      gaaggatta aacaataac tcattcaac gccgatact 
      gcatcagat gaaatggaa gagttataa
    mRNA sequence
    • >TC033447 RA
      catcgccac gaactccgt gttgaacca ttacgagag 
      gacagcgtc ctaaaattt gtccacaat atttacaat 
      aataatagt ttatatttc ccgtttttc ctgctcgtc 
      gtccgttta ttaactccg atagcatgg agaccgacg 
      agggggagt cctccagca gcggcccct tgtccagct 
      tcaacagcc tcttctcct tcaccagcc ccgcggtga 
      agaagctcc tcggctgga agcagggcg acgaggagg 
      agaagtggg cagagaagg cagtggaca gcctcgtca 
      agaagctga agaagcgca agggggcca ttgaggagc 
      tcgagcgcg cgctttcct gccccggga cgcagagca 
      actgcgtga cgatacccc gcagcctcg acggccgcc 
      tccaggtgt cccaccgca aaggcctcc cccacgtga 
      tctactgcc gggtctggc gctggcccg accttcaga 
      gccaccacg agctgaaac ctctggagc actgcaagt 
      tcccgtttt cggccaagc agaaggagg tctgcatca 
      acccctacc actacaaaa gagtggaaa gtcaagttt 
      tgccgccgg tcttggtcc cccgacaca gcgattttg 
      tcccagggc actcgctcc taccgtttc agcaagtgg 
      tggagccca acatgcccc agaatatta gttataatt 
      ccaatggat ttaattcca atcatttga gtccgagtt 
      cgccactgt cgagtgtgt ctagtccgg ggagtgttt 
      cgtcgaata atccgcaga gtccgtatt cgaatttgg 
      cggagactc cgcccccgg cctatagcc ccaccgacg 
      agaatacga ataataata cgagtccgc aaccgtcgc 
      ttaatacag atgtgcaac cggtggcct atcaggagc 
      agccctatt gggcttcta tcgcctact atgagttga 
      attgtcgcg tcggggaag ttttccatt gccattcga 
      cgtccgtta ttgttgatg ggttcacca atcccagca 
      acaacagcg acagatttt gcttagggc agctaagca 
      acgtaaacc gcaactcaa ccatcgaaa acacccgcc 
      gtcacatcg gcaaagggg tccacctat actacgtca 
      acggcgaag tttacgccg aatgtctct ccgattcgc 
      caattttcg tccaatccc gcaactgta accaccacc 
      atgggtttc acccatcca ccgtgtgta aaatcccgg 
      ctggttgtt cgttgcgta ttttcaaca acgccgaat 
      ttgcccaac tcctctccc aatgcgtga atcatggtt 
      ttgaggccg tttacgaac tcacaaaaa tgtgtacga 
      tacgtatga gcttcgtca agggttggg gcgcggagt 
      accatcgtc aggacgtca caagcaccc cctgctgga 
      tcgaggtgc atctcaacg gccccctcc agtggctcg 
      acaaggtgc tgacccaaa tggggtccc ctcacaatg 
      ccataagtt cggtctctg atttcaagc catcagctg 
      cgttaaccg aacttttca ttatccaaa agagaagaa 
      gaaaacaga aaatggttg attcatgta acattggag 
      atggtgaaa taaccatgg ttgatggaa atccattca 
      aaacagtta aaggaaata taacagttg tgaaaattg 
      aagataaat ttcaaattg aagtcaatt ctggtggca 
      acagacaac tagttattc catgtgaag aagtcatca 
      gtcgcagca tacagaacg aaactgtga ttttacaat 
      ttaaagagg ataatctct tcgaaaaag gcatttgta 
      aattttatg ataatagac aagcaattg ttttcaatt 
      ccaaactgc cttctatta tgaaacgaa catccacaa 
      accacaatt cgtctacta gttctgact cagacttta 
      atgactcag gaaacggaa aaactgaag gattaaaca 
      ataactcat tcaacgccg atactgcat cagatgaaa 
      tggaagagt tataaagtt agtcattta taaacagtt 
      ttttgaata aattttttt tcaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY